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The map label for this gene is hupS [H]

Identifier: 146338750

GI number: 146338750

Start: 1801818

End: 1802915

Strand: Direct

Name: hupS [H]

Synonym: BRADO1684

Alternate gene names: 146338750

Gene position: 1801818-1802915 (Clockwise)

Preceding gene: 146338749

Following gene: 146338751

Centisome position: 24.16

GC content: 64.75

Gene sequence:

>1098_bases
ATGGGCGCGACGACGGAAACCTTTTACAGCGTGATCCGGCGCCAGGGCATCACGCGGCGCAGCTTCCATAAGTTCTGCAG
TCTCACTGCGACGAGCCTCGGGCTCGGGCCGCTGGCGTCGGCGCGCATTGCCAATGCGCTGGAAACCAAGCCGCGCACGC
CGGTCATCTGGATGCATGGGCTGGAATGCACCTGCTGCTCGGAGAGCTTCATCCGCTCGGCGCATCCGCTGGTGAAGGAT
GCCGTGCTGTCGATGATCTCGCTCGACTATGACGACACGATCATGGCGGCGGCTGGACAGCAGGCCGAGGAGATCCTCGA
AGAGACCCGCGCGAAGTACAAAGGCCAGTACATCCTCGCCGTCGAAGGCAATCCGCCGCTGAATGAAGGCGGCATGTTCT
GCATCGACGGCGGCAAGCCGTTCGTCGAGAAGTTGAAGGTGATGGCCGAGGATGCGATGGCGATCATCGCCTGGGGCGCG
TGCGCGTCCTGGGGCTGCGTGCAGGCGGCGAAGCCCAATCCGACGCAGGCGACGCCGATCGACAAGGTCATCACCAACAA
GCCGATCATCAAGGTGCCCGGCTGCCCGCCGATCGCGGAAGTGATGACCGGCGTCGTCACCTTCATCACCACCTTCGGAA
AATTGCCCGAGCTCGACCGCCAGGGCCGGCCGAAGATGTTCTACTCGCAGCGCATCCACGACAAATGCTATCGCCGGCCG
CATTTCGACGCCGGCCAGTTCGTCGAGGAGTGGGACGACGAGGCCGCGCGCAAGGGCTACTGCCTGTACAAGATGGGCTG
CAAGGGCCCGACCACCTACAATGCCTGTTCGACCGTGCGCTGGAACGGTGGCGTGTCGTTCCCGATCCAGTCGGGCCATG
GCTGCATCGGCTGCTCCGAGGATGGCTTCTGGGACAAGGGCTCGTTCTACGACCGGCTCACCACGATCAAGCAGTTCGGC
ATCGAGCGCAACGCGGACGAGGTCGGCATGGCCGCCGCGGGAACGGTGGGCGTCGCGGTCGCGGCGCATGCCGCGGTCAC
CGCCGTCAAGCGGCTCGCGACCAAGCGCGACGACGCCAGCGCCAAGCAAGATCATTAA

Upstream 100 bases:

>100_bases
TAAGATTCGAATCCGGCTTTGATGCTGGCCCACATCTTGCTGTCCCCTCTCGCCAGATCGCGATCGGATGATCGCCAACG
CGATAGCAGGGGGAGGACTC

Downstream 100 bases:

>100_bases
TTCAGTTCAGGGCAGGCAACGATGGGCATCCAGACTCCCAACGGCTTCAATCTCGACAATTCCGGTAAGCGCGTCGTCGT
CGATCCGCTCACGCGCATCG

Product: uptake hydrogenase small subunit

Products: NA

Alternate protein names: Hydrogenlyase; Membrane-bound hydrogenase small subunit [H]

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MGATTETFYSVIRRQGITRRSFHKFCSLTATSLGLGPLASARIANALETKPRTPVIWMHGLECTCCSESFIRSAHPLVKD
AVLSMISLDYDDTIMAAAGQQAEEILEETRAKYKGQYILAVEGNPPLNEGGMFCIDGGKPFVEKLKVMAEDAMAIIAWGA
CASWGCVQAAKPNPTQATPIDKVITNKPIIKVPGCPPIAEVMTGVVTFITTFGKLPELDRQGRPKMFYSQRIHDKCYRRP
HFDAGQFVEEWDDEAARKGYCLYKMGCKGPTTYNACSTVRWNGGVSFPIQSGHGCIGCSEDGFWDKGSFYDRLTTIKQFG
IERNADEVGMAAAGTVGVAVAAHAAVTAVKRLATKRDDASAKQDH

Sequences:

>Translated_365_residues
MGATTETFYSVIRRQGITRRSFHKFCSLTATSLGLGPLASARIANALETKPRTPVIWMHGLECTCCSESFIRSAHPLVKD
AVLSMISLDYDDTIMAAAGQQAEEILEETRAKYKGQYILAVEGNPPLNEGGMFCIDGGKPFVEKLKVMAEDAMAIIAWGA
CASWGCVQAAKPNPTQATPIDKVITNKPIIKVPGCPPIAEVMTGVVTFITTFGKLPELDRQGRPKMFYSQRIHDKCYRRP
HFDAGQFVEEWDDEAARKGYCLYKMGCKGPTTYNACSTVRWNGGVSFPIQSGHGCIGCSEDGFWDKGSFYDRLTTIKQFG
IERNADEVGMAAAGTVGVAVAAHAAVTAVKRLATKRDDASAKQDH
>Mature_364_residues
GATTETFYSVIRRQGITRRSFHKFCSLTATSLGLGPLASARIANALETKPRTPVIWMHGLECTCCSESFIRSAHPLVKDA
VLSMISLDYDDTIMAAAGQQAEEILEETRAKYKGQYILAVEGNPPLNEGGMFCIDGGKPFVEKLKVMAEDAMAIIAWGAC
ASWGCVQAAKPNPTQATPIDKVITNKPIIKVPGCPPIAEVMTGVVTFITTFGKLPELDRQGRPKMFYSQRIHDKCYRRPH
FDAGQFVEEWDDEAARKGYCLYKMGCKGPTTYNACSTVRWNGGVSFPIQSGHGCIGCSEDGFWDKGSFYDRLTTIKQFGI
ERNADEVGMAAAGTVGVAVAAHAAVTAVKRLATKRDDASAKQDH

Specific function: This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions [H]

COG id: COG1740

COG function: function code C; Ni,Fe-hydrogenase I small subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787206, Length=329, Percent_Identity=72.6443768996961, Blast_Score=523, Evalue=1e-150,
Organism=Escherichia coli, GI1789371, Length=362, Percent_Identity=40.8839779005525, Blast_Score=272, Evalue=2e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR001821
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 39753; Mature: 39622

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGATTETFYSVIRRQGITRRSFHKFCSLTATSLGLGPLASARIANALETKPRTPVIWMHG
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEECC
LECTCCSESFIRSAHPLVKDAVLSMISLDYDDTIMAAAGQQAEEILEETRAKYKGQYILA
CCEEEHHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHCCCEEEE
VEGNPPLNEGGMFCIDGGKPFVEKLKVMAEDAMAIIAWGACASWGCVQAAKPNPTQATPI
ECCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCCH
DKVITNKPIIKVPGCPPIAEVMTGVVTFITTFGKLPELDRQGRPKMFYSQRIHDKCYRRP
HHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCC
HFDAGQFVEEWDDEAARKGYCLYKMGCKGPTTYNACSTVRWNGGVSFPIQSGHGCIGCSE
CCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCEEEECCCEECEECCCCCEEECCC
DGFWDKGSFYDRLTTIKQFGIERNADEVGMAAAGTVGVAVAAHAAVTAVKRLATKRDDAS
CCCCCCCHHHHHHHHHHHHCCCCCCHHHCCHHCCHHHHHHHHHHHHHHHHHHHHCCCCCC
AKQDH
CCCCC
>Mature Secondary Structure 
GATTETFYSVIRRQGITRRSFHKFCSLTATSLGLGPLASARIANALETKPRTPVIWMHG
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEECC
LECTCCSESFIRSAHPLVKDAVLSMISLDYDDTIMAAAGQQAEEILEETRAKYKGQYILA
CCEEEHHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHCCCEEEE
VEGNPPLNEGGMFCIDGGKPFVEKLKVMAEDAMAIIAWGACASWGCVQAAKPNPTQATPI
ECCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCCH
DKVITNKPIIKVPGCPPIAEVMTGVVTFITTFGKLPELDRQGRPKMFYSQRIHDKCYRRP
HHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCC
HFDAGQFVEEWDDEAARKGYCLYKMGCKGPTTYNACSTVRWNGGVSFPIQSGHGCIGCSE
CCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCEEEECCCEECEECCCCCEEECCC
DGFWDKGSFYDRLTTIKQFGIERNADEVGMAAAGTVGVAVAAHAAVTAVKRLATKRDDAS
CCCCCCCHHHHHHHHHHHHCCCCCCHHHCCHHCCHHHHHHHHHHHHHHHHHHHHCCCCCC
AKQDH
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 3054886; 12597275 [H]