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The map label for this gene is phoB [H]

Identifier: 146338745

GI number: 146338745

Start: 1795715

End: 1796422

Strand: Direct

Name: phoB [H]

Synonym: BRADO1679

Alternate gene names: 146338745

Gene position: 1795715-1796422 (Clockwise)

Preceding gene: 146338744

Following gene: 146338746

Centisome position: 24.08

GC content: 69.07

Gene sequence:

>708_bases
ATGGGCGCGCGAATTCTGGTGGTTGAGGACGAGGAGGCGCTGACGACGCTGCTGCGCTACAACCTCGAAGCCGAAGGCTA
CGAGGTCGAGACCGTGGCGCGCGGCGACGAGGCCGACACGCGGCTGAAGGAGCGCATTCCCGATCTGGTCGTGCTCGACT
GGATGCTGCCGGGCCTCTCAGGGATCGAGCTGTGCCGGCGGCTGCGGGCGCGGTCCGAGACCAAGCAGCTGCCGATCATC
ATGCTGACGGCGCGCGGCGAGGAGAGCGAGCGCGTGCGCGGTCTTGCGACCGGCGCCGATGACTACATCGTCAAGCCGTT
CTCGGTGCCGGAGCTCTTGGCGCGTGTGAAGGGCCTGCTGCGGCGCGCCAGCCCGGAGCGGCTGGCGACCGTGCTGTCCT
ATGGCGACATCGAGCTCGATCGCGAGAAGCGCCGCGTGGCGCGGTCGGGCCGGCCGATCGATCTCGGCCCGACCGAATAT
CGCCTGCTCGAGTTCTTCCTGGAGCATCCCGGCCGCGTGTTCAGCCGCGAGCAGCTGCTCGACAGCGTCTGGGGCCGCGA
CATCTATATCGACGAGCGCACGGTCGACGTCCACATCGGCCGGCTGCGCAAGCTGCTCAATCCCGGCCGCGAGCAGGATC
CGATCCGCACCGTCCGCGGCGCCGGCTATGCGCTCGACGACCGGTTCGCCAAGTCTGAGCAGGCCTGA

Upstream 100 bases:

>100_bases
TATATGATCGAGGGCCAGCAGATCATGGACAAGCGCCCCAAGGGCGACATGACCAGTTTTGCGACCACGATCCCCGGCAA
CTGAGAAAGACGATTCCGAC

Downstream 100 bases:

>100_bases
TCGCGCGGTCTGCGCGGTCACGCCGCTGCCGTCTTGATCATGTGTGATGGCGTTCCCATCATCATGGTCGGGCCTCGGCA
GACACAATGATCAAGACGCT

Product: two-component regulatory system response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 235; Mature: 234

Protein sequence:

>235_residues
MGARILVVEDEEALTTLLRYNLEAEGYEVETVARGDEADTRLKERIPDLVVLDWMLPGLSGIELCRRLRARSETKQLPII
MLTARGEESERVRGLATGADDYIVKPFSVPELLARVKGLLRRASPERLATVLSYGDIELDREKRRVARSGRPIDLGPTEY
RLLEFFLEHPGRVFSREQLLDSVWGRDIYIDERTVDVHIGRLRKLLNPGREQDPIRTVRGAGYALDDRFAKSEQA

Sequences:

>Translated_235_residues
MGARILVVEDEEALTTLLRYNLEAEGYEVETVARGDEADTRLKERIPDLVVLDWMLPGLSGIELCRRLRARSETKQLPII
MLTARGEESERVRGLATGADDYIVKPFSVPELLARVKGLLRRASPERLATVLSYGDIELDREKRRVARSGRPIDLGPTEY
RLLEFFLEHPGRVFSREQLLDSVWGRDIYIDERTVDVHIGRLRKLLNPGREQDPIRTVRGAGYALDDRFAKSEQA
>Mature_234_residues
GARILVVEDEEALTTLLRYNLEAEGYEVETVARGDEADTRLKERIPDLVVLDWMLPGLSGIELCRRLRARSETKQLPIIM
LTARGEESERVRGLATGADDYIVKPFSVPELLARVKGLLRRASPERLATVLSYGDIELDREKRRVARSGRPIDLGPTEYR
LLEFFLEHPGRVFSREQLLDSVWGRDIYIDERTVDVHIGRLRKLLNPGREQDPIRTVRGAGYALDDRFAKSEQA

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=229, Percent_Identity=50.6550218340611, Blast_Score=238, Evalue=2e-64,
Organism=Escherichia coli, GI1790860, Length=229, Percent_Identity=42.7947598253275, Blast_Score=163, Evalue=1e-41,
Organism=Escherichia coli, GI1788394, Length=222, Percent_Identity=43.2432432432432, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI1789809, Length=233, Percent_Identity=39.9141630901288, Blast_Score=158, Evalue=2e-40,
Organism=Escherichia coli, GI1786784, Length=223, Percent_Identity=39.9103139013453, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI1786911, Length=229, Percent_Identity=39.7379912663755, Blast_Score=137, Evalue=4e-34,
Organism=Escherichia coli, GI1787375, Length=226, Percent_Identity=36.7256637168142, Blast_Score=137, Evalue=6e-34,
Organism=Escherichia coli, GI1789402, Length=225, Percent_Identity=35.1111111111111, Blast_Score=134, Evalue=6e-33,
Organism=Escherichia coli, GI87082012, Length=223, Percent_Identity=36.322869955157, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI2367329, Length=230, Percent_Identity=37.8260869565217, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1790552, Length=222, Percent_Identity=36.9369369369369, Blast_Score=124, Evalue=4e-30,
Organism=Escherichia coli, GI1790863, Length=229, Percent_Identity=37.117903930131, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI1787229, Length=232, Percent_Identity=36.6379310344828, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI145693140, Length=234, Percent_Identity=33.3333333333333, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1788550, Length=142, Percent_Identity=30.2816901408451, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1788191, Length=120, Percent_Identity=28.3333333333333, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1790299, Length=113, Percent_Identity=30.0884955752212, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26811; Mature: 26680

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGARILVVEDEEALTTLLRYNLEAEGYEVETVARGDEADTRLKERIPDLVVLDWMLPGLS
CCCEEEEEECHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHCCCEEEHHHHHCCCC
GIELCRRLRARSETKQLPIIMLTARGEESERVRGLATGADDYIVKPFSVPELLARVKGLL
HHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHH
RRASPERLATVLSYGDIELDREKRRVARSGRPIDLGPTEYRLLEFFLEHPGRVFSREQLL
HHCCHHHHHHHHHHCCCEECHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHH
DSVWGRDIYIDERTVDVHIGRLRKLLNPGREQDPIRTVRGAGYALDDRFAKSEQA
HHHCCCEEEEECCEEEHHHHHHHHHHCCCCCCCHHHHHHCCCCCHHHHHHHCCCC
>Mature Secondary Structure 
GARILVVEDEEALTTLLRYNLEAEGYEVETVARGDEADTRLKERIPDLVVLDWMLPGLS
CCEEEEEECHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHCCCEEEHHHHHCCCC
GIELCRRLRARSETKQLPIIMLTARGEESERVRGLATGADDYIVKPFSVPELLARVKGLL
HHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHH
RRASPERLATVLSYGDIELDREKRRVARSGRPIDLGPTEYRLLEFFLEHPGRVFSREQLL
HHCCHHHHHHHHHHCCCEECHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHH
DSVWGRDIYIDERTVDVHIGRLRKLLNPGREQDPIRTVRGAGYALDDRFAKSEQA
HHHCCCEEEEECCEEEHHHHHHHHHHCCCCCCCHHHHHHCCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]