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The map label for this gene is potD [C]

Identifier: 146338614

GI number: 146338614

Start: 1650038

End: 1651069

Strand: Direct

Name: potD [C]

Synonym: BRADO1542

Alternate gene names: 146338614

Gene position: 1650038-1651069 (Clockwise)

Preceding gene: 146338613

Following gene: 146338615

Centisome position: 22.13

GC content: 64.92

Gene sequence:

>1032_bases
ATGATTTCGCGACGTCACTTTCTCTCCGCCAGCGCCGGCATGGCCGCGCTGGCTTCCGGCATGCCGACGGCCCGCGCCGA
GGGCGGACAGGTCGTGGTCGGCACCTGGGGCGGCGACTATGGCCAGTTGCTGTCGGATATCATCGACAAGCCTATCATGA
CGCCGAAGGGTTTTGAGATCGTGCAGGACGTCGGCAATGCCGATCCGCGCAAGACCAAGCTGCTCGCCGAGCGGCAGAGC
CGGCGCGGCTCGATGGACGTCTCCTGCCTGTCCGACAACGACGCCTACATCGTGTCGCAGTCCGACGTGTTCGAGAAGAT
CGACGCGGCCCAGGTGAAGCGGCTCGACAAGGTGTTCCCGGAGCTGAAGAGCGAGATCGCGATCCCGCACATCTATTCGG
CGCAGGTCATCCTCTACAACACCAACCAGGTGAAGACGCCGCCGCAATCCTTCGCGGATCTCTGGGATCCGAAATGGCGC
GGCAAGATCGGTCTCGCCGACATTCTCTATCCCAACAACACGCTGGCCGCGGCGCTCGCCGGCGGCGGCGGCGTCAGCAA
TCTCGATCCGGCCGAGAAGAAGCTGATGGAATGGCGCTCGCTCGACGTGAAGATCTATCCGTCGAACGAGGCGCTGGCGG
CGGCGCTGAAGTCGGAGGAGGTGTGGCTGACCACGATGTGGCTCGCGCGCGGCTTCATGTGGAAGAAGTCCGGCATTCCG
CTCGCCCACGTCGTGCCGAAGGAAGGCACGCCCGCGATCGTGTTCCAGGCCTCGGTGCCGAAGAACGCCAAGAACAAGGC
CGGCGGCTTCGCCTATCTCGACGCGATGCTCGACGCCAGGGCGCAAGGCGGCTTCGCCGACAAGATGGGCTATGTCCCGA
CCGTCACCGACGCGCCGCTGCCTGAAGATCTTGCCAAGCAGGTCAGCCTGACCGAGGCCGAGCGCGCGCGGCTCTTGAAA
CCGGACTACGCCTATGCCGCCGGGCGCGCGCAGCGCACGCTCGATTTCTGGAACAAGGAGTTCAAGGCCTGA

Upstream 100 bases:

>100_bases
GCCCGACGATACCGTGTCGCTGCAATGGGACCGCAAGGTCCCGCGTCTCGTGCCGCAAACATCCAAGTAGAAGTATTCAT
CCCAAGCGAGAGGTTCTCCC

Downstream 100 bases:

>100_bases
GCCTTGCCTCATCGCTCCTGCGGCCTCGGCCGCAGGAGCACGCGGCCGATCGGCCGCTCATCTGCAATCGGAGCTCGCGC
GATGGCGCTGCTGGTCGTTC

Product: ABC transporter substrate-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: ABC Transporter; Periplasmic Component Of Efflux System

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MISRRHFLSASAGMAALASGMPTARAEGGQVVVGTWGGDYGQLLSDIIDKPIMTPKGFEIVQDVGNADPRKTKLLAERQS
RRGSMDVSCLSDNDAYIVSQSDVFEKIDAAQVKRLDKVFPELKSEIAIPHIYSAQVILYNTNQVKTPPQSFADLWDPKWR
GKIGLADILYPNNTLAAALAGGGGVSNLDPAEKKLMEWRSLDVKIYPSNEALAAALKSEEVWLTTMWLARGFMWKKSGIP
LAHVVPKEGTPAIVFQASVPKNAKNKAGGFAYLDAMLDARAQGGFADKMGYVPTVTDAPLPEDLAKQVSLTEAERARLLK
PDYAYAAGRAQRTLDFWNKEFKA

Sequences:

>Translated_343_residues
MISRRHFLSASAGMAALASGMPTARAEGGQVVVGTWGGDYGQLLSDIIDKPIMTPKGFEIVQDVGNADPRKTKLLAERQS
RRGSMDVSCLSDNDAYIVSQSDVFEKIDAAQVKRLDKVFPELKSEIAIPHIYSAQVILYNTNQVKTPPQSFADLWDPKWR
GKIGLADILYPNNTLAAALAGGGGVSNLDPAEKKLMEWRSLDVKIYPSNEALAAALKSEEVWLTTMWLARGFMWKKSGIP
LAHVVPKEGTPAIVFQASVPKNAKNKAGGFAYLDAMLDARAQGGFADKMGYVPTVTDAPLPEDLAKQVSLTEAERARLLK
PDYAYAAGRAQRTLDFWNKEFKA
>Mature_343_residues
MISRRHFLSASAGMAALASGMPTARAEGGQVVVGTWGGDYGQLLSDIIDKPIMTPKGFEIVQDVGNADPRKTKLLAERQS
RRGSMDVSCLSDNDAYIVSQSDVFEKIDAAQVKRLDKVFPELKSEIAIPHIYSAQVILYNTNQVKTPPQSFADLWDPKWR
GKIGLADILYPNNTLAAALAGGGGVSNLDPAEKKLMEWRSLDVKIYPSNEALAAALKSEEVWLTTMWLARGFMWKKSGIP
LAHVVPKEGTPAIVFQASVPKNAKNKAGGFAYLDAMLDARAQGGFADKMGYVPTVTDAPLPEDLAKQVSLTEAERARLLK
PDYAYAAGRAQRTLDFWNKEFKA

Specific function: Required For The Activity Of The Bacterial Periplasmic Transport System Of Putrescine And Spermidine. Polyamine Binding Protein. [C]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787367, Length=261, Percent_Identity=24.904214559387, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 37418; Mature: 37418

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISRRHFLSASAGMAALASGMPTARAEGGQVVVGTWGGDYGQLLSDIIDKPIMTPKGFEI
CCCCCCHHCCCCCHHHHHCCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCHHH
VQDVGNADPRKTKLLAERQSRRGSMDVSCLSDNDAYIVSQSDVFEKIDAAQVKRLDKVFP
HHHHCCCCHHHHHHHHHHHHCCCCCEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHH
ELKSEIAIPHIYSAQVILYNTNQVKTPPQSFADLWDPKWRGKIGLADILYPNNTLAAALA
HHHHHCCCCEEEECEEEEEECCCCCCCHHHHHHHCCCCCCCCCCEEEEECCCCCEEEEEE
GGGGVSNLDPAEKKLMEWRSLDVKIYPSNEALAAALKSEEVWLTTMWLARGFMWKKSGIP
CCCCCCCCCHHHHHHHHHHCCCEEEECCCHHHHHHHCCCCEEEHHHHHHHCCEEECCCCC
LAHVVPKEGTPAIVFQASVPKNAKNKAGGFAYLDAMLDARAQGGFADKMGYVPTVTDAPL
EEEECCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCC
PEDLAKQVSLTEAERARLLKPDYAYAAGRAQRTLDFWNKEFKA
CHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MISRRHFLSASAGMAALASGMPTARAEGGQVVVGTWGGDYGQLLSDIIDKPIMTPKGFEI
CCCCCCHHCCCCCHHHHHCCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCHHH
VQDVGNADPRKTKLLAERQSRRGSMDVSCLSDNDAYIVSQSDVFEKIDAAQVKRLDKVFP
HHHHCCCCHHHHHHHHHHHHCCCCCEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHH
ELKSEIAIPHIYSAQVILYNTNQVKTPPQSFADLWDPKWRGKIGLADILYPNNTLAAALA
HHHHHCCCCEEEECEEEEEECCCCCCCHHHHHHHCCCCCCCCCCEEEEECCCCCEEEEEE
GGGGVSNLDPAEKKLMEWRSLDVKIYPSNEALAAALKSEEVWLTTMWLARGFMWKKSGIP
CCCCCCCCCHHHHHHHHHHCCCEEEECCCHHHHHHHCCCCEEEHHHHHHHCCEEECCCCC
LAHVVPKEGTPAIVFQASVPKNAKNKAGGFAYLDAMLDARAQGGFADKMGYVPTVTDAPL
EEEECCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCC
PEDLAKQVSLTEAERARLLKPDYAYAAGRAQRTLDFWNKEFKA
CHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA