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The map label for this gene is potB [H]

Identifier: 146338615

GI number: 146338615

Start: 1651151

End: 1651981

Strand: Direct

Name: potB [H]

Synonym: BRADO1543

Alternate gene names: 146338615

Gene position: 1651151-1651981 (Clockwise)

Preceding gene: 146338614

Following gene: 146338616

Centisome position: 22.14

GC content: 63.9

Gene sequence:

>831_bases
ATGGCGCTGCTGGTCGTTCCTGCCTGTCTTCTGGTCCTGGCGCTCCTGATCGGGCCGTTGATCCTGATGTTCCGGATCTC
GCTCAACCAGTTCAGCCCGACCCAGCTGATGGTGCAGGCGCTGTCGCCGGACAACTACATCAAGGCTGCGACGGATCCCT
ACTACCAGCAGATCATCCTGGCGACGCTCGGCATCTCCATGCTGTGCACCTTGCTGACGCTGATCCTGGCGTTTCCGGCG
GCCTACTGGATCGGCCGCATGGAGAGCCGCTGGAAGAGCCTGGTCGTGATCGCGACCTTGTTCCCGCTGCTGGTCGGCAA
CGTCGTGCGCTCCGCCGGCTGGATGGCGCTGTTCGCGCGCGACGGCCTCGTCAACACGGGTCTCTTGAAACTCGGCCTGA
TCTCGACGCCGCTCGAGATCATGTTCACGACCAAGGCCGTCATCATCGGCATCATCGCGGTGGTGCTGCCCTACATGATC
CTGACGCTGTCGGCCGTCATCGAGAGCATACCCCGCGACCTGGAATATGCCGCCGCCAATCTCGGCGCCTCGGGCGCGCG
GGTGTTCTGGCGCGTGATTCTGCCGCTGTCCGGTCCCGGCGTCGCCGCCGGCTCGATCCTGGTGTTCGTTTTGTGCATGA
ACACCTATGCAACGGCGGTGCTTCTCGGCGGCCCGCGATTCAAGATGATGGCGCCCGCGGTGTACGATCAGTTCGTGCGC
GGTAACAACTGGCCAATGGGCGCCGCGCTCGCCTTCATGCTGCTCGCGGTGACCATGGCGTTCACGGTGTTCGGCAGCCT
AGCGTTCGCGCGGAAGTATCGGACGCAATGA

Upstream 100 bases:

>100_bases
CAAGGAGTTCAAGGCCTGAGCCTTGCCTCATCGCTCCTGCGGCCTCGGCCGCAGGAGCACGCGGCCGATCGGCCGCTCAT
CTGCAATCGGAGCTCGCGCG

Downstream 100 bases:

>100_bases
CGGCAGACGCCGTCATTGCGAGGAACGCAGCGACGAAGCAATCCAGACTTTCCTTGGAGCCCTGGATTGCTTCGCTGCGC
TCGCAATGACGAGAAGACGG

Product: ABC transporter permease

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 275

Protein sequence:

>276_residues
MALLVVPACLLVLALLIGPLILMFRISLNQFSPTQLMVQALSPDNYIKAATDPYYQQIILATLGISMLCTLLTLILAFPA
AYWIGRMESRWKSLVVIATLFPLLVGNVVRSAGWMALFARDGLVNTGLLKLGLISTPLEIMFTTKAVIIGIIAVVLPYMI
LTLSAVIESIPRDLEYAAANLGASGARVFWRVILPLSGPGVAAGSILVFVLCMNTYATAVLLGGPRFKMMAPAVYDQFVR
GNNWPMGAALAFMLLAVTMAFTVFGSLAFARKYRTQ

Sequences:

>Translated_276_residues
MALLVVPACLLVLALLIGPLILMFRISLNQFSPTQLMVQALSPDNYIKAATDPYYQQIILATLGISMLCTLLTLILAFPA
AYWIGRMESRWKSLVVIATLFPLLVGNVVRSAGWMALFARDGLVNTGLLKLGLISTPLEIMFTTKAVIIGIIAVVLPYMI
LTLSAVIESIPRDLEYAAANLGASGARVFWRVILPLSGPGVAAGSILVFVLCMNTYATAVLLGGPRFKMMAPAVYDQFVR
GNNWPMGAALAFMLLAVTMAFTVFGSLAFARKYRTQ
>Mature_275_residues
ALLVVPACLLVLALLIGPLILMFRISLNQFSPTQLMVQALSPDNYIKAATDPYYQQIILATLGISMLCTLLTLILAFPAA
YWIGRMESRWKSLVVIATLFPLLVGNVVRSAGWMALFARDGLVNTGLLKLGLISTPLEIMFTTKAVIIGIIAVVLPYMIL
TLSAVIESIPRDLEYAAANLGASGARVFWRVILPLSGPGVAAGSILVFVLCMNTYATAVLLGGPRFKMMAPAVYDQFVRG
NNWPMGAALAFMLLAVTMAFTVFGSLAFARKYRTQ

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=206, Percent_Identity=34.4660194174757, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1787080, Length=229, Percent_Identity=29.6943231441048, Blast_Score=121, Evalue=6e-29,
Organism=Escherichia coli, GI1787713, Length=232, Percent_Identity=34.051724137931, Blast_Score=102, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29883; Mature: 29751

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALLVVPACLLVLALLIGPLILMFRISLNQFSPTQLMVQALSPDNYIKAATDPYYQQIIL
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCEECCCCHHHHHHHH
ATLGISMLCTLLTLILAFPAAYWIGRMESRWKSLVVIATLFPLLVGNVVRSAGWMALFAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
DGLVNTGLLKLGLISTPLEIMFTTKAVIIGIIAVVLPYMILTLSAVIESIPRDLEYAAAN
CCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
LGASGARVFWRVILPLSGPGVAAGSILVFVLCMNTYATAVLLGGPRFKMMAPAVYDQFVR
CCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHC
GNNWPMGAALAFMLLAVTMAFTVFGSLAFARKYRTQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ALLVVPACLLVLALLIGPLILMFRISLNQFSPTQLMVQALSPDNYIKAATDPYYQQIIL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCEECCCCHHHHHHHH
ATLGISMLCTLLTLILAFPAAYWIGRMESRWKSLVVIATLFPLLVGNVVRSAGWMALFAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
DGLVNTGLLKLGLISTPLEIMFTTKAVIIGIIAVVLPYMILTLSAVIESIPRDLEYAAAN
CCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
LGASGARVFWRVILPLSGPGVAAGSILVFVLCMNTYATAVLLGGPRFKMMAPAVYDQFVR
CCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHC
GNNWPMGAALAFMLLAVTMAFTVFGSLAFARKYRTQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]