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The map label for this gene is potI [H]

Identifier: 146338612

GI number: 146338612

Start: 1648098

End: 1648943

Strand: Direct

Name: potI [H]

Synonym: BRADO1540

Alternate gene names: 146338612

Gene position: 1648098-1648943 (Clockwise)

Preceding gene: 146338605

Following gene: 146338613

Centisome position: 22.1

GC content: 65.01

Gene sequence:

>846_bases
TTGTTTGTTGAGGTGTCTGCGATGCTGTTCCGTTTTACGGGACACCTGTCTATCGGCTCTTTCGCAGTGCGGACCCTGAT
CGTCGTCGCCTATGCCATCATCTTCGCGCCGGTCGTGATGATCGTGATGACCAGCTTCTTCGCGCAGGAGATCGTCAGCT
TCCCGCCGCAGGCGCTGTCACTGAAATGGTACGCGAACGCGCTCAGCAAGCCGGAATTCCTGCGCGGCCTTGTCACCAGC
TTCCAGGTGGCGCTGCTTGCGACGGCCATCGGCGTGCCGCTCGGCACCGCCGCGGCGCTTGCCATCGTGCGCGGCGAATT
CCGCGGGCGGAAGGCGCTGTCGTCGTTCCTGCTGGCGCCGCTCGCGGTGCCCGGCGTGGTCGCCGGTTCCGGGCTCTACA
TGTTTTACGTGCTGGCCGAGGATCTCCTCGACCGCGACATCAAGGCGACGACGGAGGGCCTCGTCGCCGCGCATACGCTG
TTGGCCATTCCATGGACGGTGCGCCTCGTGGTCGCCAGCCTGCAAGGCCTCGACCGCGCCGCGGAGGAGGCCGCCGCCAA
TCTCGGCGCCAGCCCGTTCACGGTGTTCCGCCGCATCACGCTGCCGATGATGCGCTCGGGGATCGTGGCGGCCGCGATGT
TTTCCTTCATTCAGAGTTTTGAGAATCTCGATCTGTCGATGCTGCTGGTCGGACCCGGACGGATCACGCTTCCCGTGGCC
ATGCTCAACTACCTCGAATTCCGCATCGATCCGACGCTCGCCGCCGTCGCCACCGTGCAGATCGCGCTGGTCGGCCTGCT
GATGGTGATCACCGACCGCTTCGTCAAGCTGTCGCGGGTGGTGTGA

Upstream 100 bases:

>100_bases
GCATGTCGCGTGCCCATGCGCATGTGCATCGCGATAAGCCCTCCCGCGGGCTTGGCGGGCGCGGAGCCGCCGACTACGAT
GCCGCCGCACAAGCTGTCCT

Downstream 100 bases:

>100_bases
TGGCGCAACTCACTCTCGAGAAGCTCACCAAGCGCTTCGGCACATCGATCGGCGTCGAGAGCCTCGATCTCTCGGTCAAC
CAGGGCGAGCTCGTTGCGCT

Product: ABC transporter permease

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MFVEVSAMLFRFTGHLSIGSFAVRTLIVVAYAIIFAPVVMIVMTSFFAQEIVSFPPQALSLKWYANALSKPEFLRGLVTS
FQVALLATAIGVPLGTAAALAIVRGEFRGRKALSSFLLAPLAVPGVVAGSGLYMFYVLAEDLLDRDIKATTEGLVAAHTL
LAIPWTVRLVVASLQGLDRAAEEAAANLGASPFTVFRRITLPMMRSGIVAAAMFSFIQSFENLDLSMLLVGPGRITLPVA
MLNYLEFRIDPTLAAVATVQIALVGLLMVITDRFVKLSRVV

Sequences:

>Translated_281_residues
MFVEVSAMLFRFTGHLSIGSFAVRTLIVVAYAIIFAPVVMIVMTSFFAQEIVSFPPQALSLKWYANALSKPEFLRGLVTS
FQVALLATAIGVPLGTAAALAIVRGEFRGRKALSSFLLAPLAVPGVVAGSGLYMFYVLAEDLLDRDIKATTEGLVAAHTL
LAIPWTVRLVVASLQGLDRAAEEAAANLGASPFTVFRRITLPMMRSGIVAAAMFSFIQSFENLDLSMLLVGPGRITLPVA
MLNYLEFRIDPTLAAVATVQIALVGLLMVITDRFVKLSRVV
>Mature_281_residues
MFVEVSAMLFRFTGHLSIGSFAVRTLIVVAYAIIFAPVVMIVMTSFFAQEIVSFPPQALSLKWYANALSKPEFLRGLVTS
FQVALLATAIGVPLGTAAALAIVRGEFRGRKALSSFLLAPLAVPGVVAGSGLYMFYVLAEDLLDRDIKATTEGLVAAHTL
LAIPWTVRLVVASLQGLDRAAEEAAANLGASPFTVFRRITLPMMRSGIVAAAMFSFIQSFENLDLSMLLVGPGRITLPVA
MLNYLEFRIDPTLAAVATVQIALVGLLMVITDRFVKLSRVV

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787081, Length=243, Percent_Identity=28.8065843621399, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1787368, Length=219, Percent_Identity=27.3972602739726, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1787714, Length=210, Percent_Identity=31.9047619047619, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1787369, Length=252, Percent_Identity=25.3968253968254, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30213; Mature: 30213

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: PS00435 PEROXIDASE_1 ; PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFVEVSAMLFRFTGHLSIGSFAVRTLIVVAYAIIFAPVVMIVMTSFFAQEIVSFPPQALS
CEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
LKWYANALSKPEFLRGLVTSFQVALLATAIGVPLGTAAALAIVRGEFRGRKALSSFLLAP
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHH
LAVPGVVAGSGLYMFYVLAEDLLDRDIKATTEGLVAAHTLLAIPWTVRLVVASLQGLDRA
HHCCCHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEEAAANLGASPFTVFRRITLPMMRSGIVAAAMFSFIQSFENLDLSMLLVGPGRITLPVA
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHH
MLNYLEFRIDPTLAAVATVQIALVGLLMVITDRFVKLSRVV
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFVEVSAMLFRFTGHLSIGSFAVRTLIVVAYAIIFAPVVMIVMTSFFAQEIVSFPPQALS
CEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
LKWYANALSKPEFLRGLVTSFQVALLATAIGVPLGTAAALAIVRGEFRGRKALSSFLLAP
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHH
LAVPGVVAGSGLYMFYVLAEDLLDRDIKATTEGLVAAHTLLAIPWTVRLVVASLQGLDRA
HHCCCHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEEAAANLGASPFTVFRRITLPMMRSGIVAAAMFSFIQSFENLDLSMLLVGPGRITLPVA
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHH
MLNYLEFRIDPTLAAVATVQIALVGLLMVITDRFVKLSRVV
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]