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The map label for this gene is pilC [H]

Identifier: 146338382

GI number: 146338382

Start: 1386919

End: 1388088

Strand: Direct

Name: pilC [H]

Synonym: BRADO1294

Alternate gene names: 146338382

Gene position: 1386919-1388088 (Clockwise)

Preceding gene: 146338381

Following gene: 146338383

Centisome position: 18.6

GC content: 66.24

Gene sequence:

>1170_bases
GTGCATGGGACGATCTCGGCCGCGACAGCGAAGGATGTCATCGACCGGATCGAATATCTGCGTCTGTTGCCGATCGAGAC
GATCGAAGAGAAGATCGGCAGCTCGGTCAGACGTGACATGGCGGCCTGGCTTGGCGGCCCTGGCCCGGGCGGTGTCACGA
CGTTCACGCGCGACCTGGCGCTGCTCCTGAAAGCCGGCGCACGGCTGGAGGACGCGCTCGAGCTTCTGGCCGGCGACGCC
GATATCGGGCGGCTGCGTGCGATCGTCAGCCGGCTGCGATCCAGCATTCTTGCGGGCGAGAGCTTTGCCGATGCGCTGGC
GCATCATCCGACGCAGTTTCCTCCGATGTATCTGGCGCTGGTGCGGGTCGGCGAGGCCTCGGGCAGCCTCGATCACGTGC
TCGACGTGCTCGGCGCGGAGCGGGCGCGGGCGGAGGCGATGCGTCGCAAGCTCGGAGATGCGCTGCAATATCCGCTATTC
GTCCTGGTCGCCGCCTTCCTGGTGATGCTGTTCTTCTTCATCGTCGTGCTGCCGCAGTTCTCGACCGTGCTGCGCGATTT
CGGCGCGAGGACGGACGCGGCCATCACGACGTTTCTCGACATCTCCGAATTCCTCCGCAGCAACGGCGCGGCGATCGCGT
TATTGGGAAGCGGAATCGTCGCCGCGGGTATCCTGCTGCTCGGGCGTGCAAGCATTCGAGGCGCGGTGATCGCGCGTCTG
TGCCGCCTGCCGGGTCTTGCGGCCGTGTTCGGGTCGTATCGCACCAGCGTGTTCTGCCGCAATCTGGCAATCCTGCTCGG
TAGCGGCCTGACCCTGACGGCGACCTTGCGCATCCTGGTCGACATCATGTCGGTCACCGGCGACGGCGCGGTTTGGCGCG
CCGCCGCTGACCGGGTGCGGCAAGGCGGCAAGCTGTCGGAGGCGCTGACGTCGGCGGACATCCTGCCGGCGATGGCCACG
CGGATGATCCGGATCGGCGAGGAGACCGGGCAATTGCCGGTTCTCGCGGGACGGATCGCTGAATTCTACGAGGCCAAGCT
GCAGCGCAGCCTCGACCGTATCGTCGGTATCGTCGGCCCCGCGGCCATCATACTCATCAGTACCGTCGTCGGCGGCCTCA
TCGTATCGGTGATGACCGCGCTGTTGTCGGTCACTCAACTCGTCGGGTAG

Upstream 100 bases:

>100_bases
GCCGGCGCGACGTCGGTCGCGGAAGTCTTGCGCGTCACGACGCTGCGGTGAGCCGATGAGCACATTTCGATATCGTGCAC
TGACCGAGAGTGGCGACGTC

Downstream 100 bases:

>100_bases
CGGAAGATGAAGCGTCGGATGACACAGGCAGGACTGAGCGGCGCCGCGCGGCAGGCGCGCAGCGACAGAGATGATCATGA
GAGCGGCTTCACGCTCGTCG

Product: putative general secretion pathway protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MHGTISAATAKDVIDRIEYLRLLPIETIEEKIGSSVRRDMAAWLGGPGPGGVTTFTRDLALLLKAGARLEDALELLAGDA
DIGRLRAIVSRLRSSILAGESFADALAHHPTQFPPMYLALVRVGEASGSLDHVLDVLGAERARAEAMRRKLGDALQYPLF
VLVAAFLVMLFFFIVVLPQFSTVLRDFGARTDAAITTFLDISEFLRSNGAAIALLGSGIVAAGILLLGRASIRGAVIARL
CRLPGLAAVFGSYRTSVFCRNLAILLGSGLTLTATLRILVDIMSVTGDGAVWRAAADRVRQGGKLSEALTSADILPAMAT
RMIRIGEETGQLPVLAGRIAEFYEAKLQRSLDRIVGIVGPAAIILISTVVGGLIVSVMTALLSVTQLVG

Sequences:

>Translated_389_residues
MHGTISAATAKDVIDRIEYLRLLPIETIEEKIGSSVRRDMAAWLGGPGPGGVTTFTRDLALLLKAGARLEDALELLAGDA
DIGRLRAIVSRLRSSILAGESFADALAHHPTQFPPMYLALVRVGEASGSLDHVLDVLGAERARAEAMRRKLGDALQYPLF
VLVAAFLVMLFFFIVVLPQFSTVLRDFGARTDAAITTFLDISEFLRSNGAAIALLGSGIVAAGILLLGRASIRGAVIARL
CRLPGLAAVFGSYRTSVFCRNLAILLGSGLTLTATLRILVDIMSVTGDGAVWRAAADRVRQGGKLSEALTSADILPAMAT
RMIRIGEETGQLPVLAGRIAEFYEAKLQRSLDRIVGIVGPAAIILISTVVGGLIVSVMTALLSVTQLVG
>Mature_389_residues
MHGTISAATAKDVIDRIEYLRLLPIETIEEKIGSSVRRDMAAWLGGPGPGGVTTFTRDLALLLKAGARLEDALELLAGDA
DIGRLRAIVSRLRSSILAGESFADALAHHPTQFPPMYLALVRVGEASGSLDHVLDVLGAERARAEAMRRKLGDALQYPLF
VLVAAFLVMLFFFIVVLPQFSTVLRDFGARTDAAITTFLDISEFLRSNGAAIALLGSGIVAAGILLLGRASIRGAVIARL
CRLPGLAAVFGSYRTSVFCRNLAILLGSGLTLTATLRILVDIMSVTGDGAVWRAAADRVRQGGKLSEALTSADILPAMAT
RMIRIGEETGQLPVLAGRIAEFYEAKLQRSLDRIVGIVGPAAIILISTVVGGLIVSVMTALLSVTQLVG

Specific function: Involved in the translocation of the type IV pilin (pilA) [H]

COG id: COG1459

COG function: function code NU; Type II secretory pathway, component PulF

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP F family [H]

Homologues:

Organism=Escherichia coli, GI1789724, Length=339, Percent_Identity=29.7935103244838, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1786295, Length=322, Percent_Identity=24.223602484472, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003004
- InterPro:   IPR018076
- InterPro:   IPR001992 [H]

Pfam domain/function: PF00482 GSPII_F [H]

EC number: NA

Molecular weight: Translated: 41168; Mature: 41168

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHGTISAATAKDVIDRIEYLRLLPIETIEEKIGSSVRRDMAAWLGGPGPGGVTTFTRDLA
CCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
LLLKAGARLEDALELLAGDADIGRLRAIVSRLRSSILAGESFADALAHHPTQFPPMYLAL
HHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHH
VRVGEASGSLDHVLDVLGAERARAEAMRRKLGDALQYPLFVLVAAFLVMLFFFIVVLPQF
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
STVLRDFGARTDAAITTFLDISEFLRSNGAAIALLGSGIVAAGILLLGRASIRGAVIARL
HHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
CRLPGLAAVFGSYRTSVFCRNLAILLGSGLTLTATLRILVDIMSVTGDGAVWRAAADRVR
HHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
QGGKLSEALTSADILPAMATRMIRIGEETGQLPVLAGRIAEFYEAKLQRSLDRIVGIVGP
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAIILISTVVGGLIVSVMTALLSVTQLVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MHGTISAATAKDVIDRIEYLRLLPIETIEEKIGSSVRRDMAAWLGGPGPGGVTTFTRDLA
CCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
LLLKAGARLEDALELLAGDADIGRLRAIVSRLRSSILAGESFADALAHHPTQFPPMYLAL
HHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHH
VRVGEASGSLDHVLDVLGAERARAEAMRRKLGDALQYPLFVLVAAFLVMLFFFIVVLPQF
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
STVLRDFGARTDAAITTFLDISEFLRSNGAAIALLGSGIVAAGILLLGRASIRGAVIARL
HHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
CRLPGLAAVFGSYRTSVFCRNLAILLGSGLTLTATLRILVDIMSVTGDGAVWRAAADRVR
HHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
QGGKLSEALTSADILPAMATRMIRIGEETGQLPVLAGRIAEFYEAKLQRSLDRIVGIVGP
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAIILISTVVGGLIVSVMTALLSVTQLVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1971619; 10984043 [H]