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The map label for this gene is nirK [H]

Identifier: 146338322

GI number: 146338322

Start: 1305001

End: 1306095

Strand: Reverse

Name: nirK [H]

Synonym: BRADO1227

Alternate gene names: 146338322

Gene position: 1306095-1305001 (Counterclockwise)

Preceding gene: 146338323

Following gene: 146338321

Centisome position: 17.52

GC content: 65.21

Gene sequence:

>1095_bases
ATGTTGACCCGCCGCACCGCTTTATTCAGTGCAGCCATCGCCGCGATGATGCTTGCGGCTCCCGCTGCTGCCGACGACCT
CAAGCTGCCGCGCCAGCGGGTCGATCTGGTCGCGCCGCCCTTCGTGCATGCGCATGAGCAGGCGACCAAGCAAGGGCCGA
AGATCATGGAGTTCAGGCTCGTGGTCCAGGAGAAGAAGATGGTGATCGACGAGAAAGGCACCACCTTCCAGGCCATGACG
TTCAACGGCTCGATGCCTGGCCCGCTGATGGTCGTGCATGAGGGCGACTATGTCGAGGTGACGCTGGTCAATCCGGCGAC
CAACACCATGCCGCACAACATCGACTTCCACGCCGCGACCGGCGCGCTCGGTGGCGGCGCCCTGACGCTGATCAACCCGG
GCGAGCAGGTCGTGCTGCGCTGGAAGGCCACCCGCACCGGCGTCTTCGTCTATCACTGCGCCCCGGGCGGCCCGATGATC
CCGTGGCACGTCGTCTCCGGCATGAACGGCGCCGTGATGGTGCTTCCGCGCGACGGTCTCAATGACGGCCATGGCCACGC
GCTGCGCTACGACCGCATCTACTACATCGGCGAGCAGGACCTCTATGTCCCGCGCGACGAGAAGGGCAATTTCAAGTCCT
ACGACTCGCCCGGCGAGGCCTACAGCGACACCGAGGAGGTGATGCGCAAGCTGACACCGACGCACGTCGTGTTCAACGGC
AGGGCGGGTGCGCTCACCGGCAAGAATGCGCTCACCGCGAATGTCGGCGAGAACGTCCTGATCGTGCACTCGCAGGCCAA
CCGCGACAGCCGCCCGCATCTGATCGGCGGCCATGGCGACTATGTCTGGGAGACCGGCAAGTTCTCCAACGCGCCGGAGA
CCGGGCTCGAGACCTGGTTCATCCGCGGCGGCTCGGCCGGTGCTGCGCTCTACAAATTCCTGCAGCCCGGCATCTACGCC
TATGTCACCCACAACCTGATCGAGGCCGCCGATCTCGGCGCCACAGCGCATTTCAAGGTCGAAGGCAAGTGGAACGACGA
CCTGATGACGCAGGTCAGGGCGCCGGCCGACATTCCGACCGGCTCGACCAACTGA

Upstream 100 bases:

>100_bases
CCGCAAATGCTCAGTTGCTTGTTGCAGCACAAACAAGCCCGGCTCCGTCGAGCGCATGCTGTTTCGCGGCACACCAACAG
ATCCAACGAAGGAGCGATCC

Downstream 100 bases:

>100_bases
TCGGATCAGGGGGCCGGCGCGACCGGCCCCCGCACTTCTCGATGTGCTGCCATGCTGCTCGCCTTCAAGATCAAGATCGC
CCTCGCCTGCCTCGTCGGCA

Product: copper-containing nitrite reductase (NO-forming) nirK

Products: NA

Alternate protein names: Cu-NIR [H]

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MLTRRTALFSAAIAAMMLAAPAAADDLKLPRQRVDLVAPPFVHAHEQATKQGPKIMEFRLVVQEKKMVIDEKGTTFQAMT
FNGSMPGPLMVVHEGDYVEVTLVNPATNTMPHNIDFHAATGALGGGALTLINPGEQVVLRWKATRTGVFVYHCAPGGPMI
PWHVVSGMNGAVMVLPRDGLNDGHGHALRYDRIYYIGEQDLYVPRDEKGNFKSYDSPGEAYSDTEEVMRKLTPTHVVFNG
RAGALTGKNALTANVGENVLIVHSQANRDSRPHLIGGHGDYVWETGKFSNAPETGLETWFIRGGSAGAALYKFLQPGIYA
YVTHNLIEAADLGATAHFKVEGKWNDDLMTQVRAPADIPTGSTN

Sequences:

>Translated_364_residues
MLTRRTALFSAAIAAMMLAAPAAADDLKLPRQRVDLVAPPFVHAHEQATKQGPKIMEFRLVVQEKKMVIDEKGTTFQAMT
FNGSMPGPLMVVHEGDYVEVTLVNPATNTMPHNIDFHAATGALGGGALTLINPGEQVVLRWKATRTGVFVYHCAPGGPMI
PWHVVSGMNGAVMVLPRDGLNDGHGHALRYDRIYYIGEQDLYVPRDEKGNFKSYDSPGEAYSDTEEVMRKLTPTHVVFNG
RAGALTGKNALTANVGENVLIVHSQANRDSRPHLIGGHGDYVWETGKFSNAPETGLETWFIRGGSAGAALYKFLQPGIYA
YVTHNLIEAADLGATAHFKVEGKWNDDLMTQVRAPADIPTGSTN
>Mature_364_residues
MLTRRTALFSAAIAAMMLAAPAAADDLKLPRQRVDLVAPPFVHAHEQATKQGPKIMEFRLVVQEKKMVIDEKGTTFQAMT
FNGSMPGPLMVVHEGDYVEVTLVNPATNTMPHNIDFHAATGALGGGALTLINPGEQVVLRWKATRTGVFVYHCAPGGPMI
PWHVVSGMNGAVMVLPRDGLNDGHGHALRYDRIYYIGEQDLYVPRDEKGNFKSYDSPGEAYSDTEEVMRKLTPTHVVFNG
RAGALTGKNALTANVGENVLIVHSQANRDSRPHLIGGHGDYVWETGKFSNAPETGLETWFIRGGSAGAALYKFLQPGIYA
YVTHNLIEAADLGATAHFKVEGKWNDDLMTQVRAPADIPTGSTN

Specific function: Unknown

COG id: COG2132

COG function: function code Q; Putative multicopper oxidases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 2 plastocyanin-like domains [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001117
- InterPro:   IPR011707
- InterPro:   IPR008972
- InterPro:   IPR001287
- InterPro:   IPR006311 [H]

Pfam domain/function: PF00394 Cu-oxidase; PF07732 Cu-oxidase_3 [H]

EC number: =1.7.2.1 [H]

Molecular weight: Translated: 39531; Mature: 39531

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTRRTALFSAAIAAMMLAAPAAADDLKLPRQRVDLVAPPFVHAHEQATKQGPKIMEFRL
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHCCCCEEEEEE
VVQEKKMVIDEKGTTFQAMTFNGSMPGPLMVVHEGDYVEVTLVNPATNTMPHNIDFHAAT
EEECCEEEEECCCCEEEEEEECCCCCCCEEEEECCCEEEEEEECCCCCCCCCCCEEEEEC
GALGGGALTLINPGEQVVLRWKATRTGVFVYHCAPGGPMIPWHVVSGMNGAVMVLPRDGL
CCCCCCEEEEECCCCEEEEEEEECCCEEEEEEECCCCCCCCHHEECCCCCEEEEEECCCC
NDGHGHALRYDRIYYIGEQDLYVPRDEKGNFKSYDSPGEAYSDTEEVMRKLTPTHVVFNG
CCCCCCEEEEEEEEEECCCCEECCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEEECC
RAGALTGKNALTANVGENVLIVHSQANRDSRPHLIGGHGDYVWETGKFSNAPETGLETWF
CCCEEECCCCEEECCCCCEEEEEECCCCCCCCCEECCCCCEEEECCCCCCCCCCCCEEEE
IRGGSAGAALYKFLQPGIYAYVTHNLIEAADLGATAHFKVEGKWNDDLMTQVRAPADIPT
EECCCCHHHHHHHHCCCEEEEEHHHHHHHHHCCCEEEEEEECCCCHHHHHHHCCCCCCCC
GSTN
CCCC
>Mature Secondary Structure
MLTRRTALFSAAIAAMMLAAPAAADDLKLPRQRVDLVAPPFVHAHEQATKQGPKIMEFRL
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHCCCCEEEEEE
VVQEKKMVIDEKGTTFQAMTFNGSMPGPLMVVHEGDYVEVTLVNPATNTMPHNIDFHAAT
EEECCEEEEECCCCEEEEEEECCCCCCCEEEEECCCEEEEEEECCCCCCCCCCCEEEEEC
GALGGGALTLINPGEQVVLRWKATRTGVFVYHCAPGGPMIPWHVVSGMNGAVMVLPRDGL
CCCCCCEEEEECCCCEEEEEEEECCCEEEEEEECCCCCCCCHHEECCCCCEEEEEECCCC
NDGHGHALRYDRIYYIGEQDLYVPRDEKGNFKSYDSPGEAYSDTEEVMRKLTPTHVVFNG
CCCCCCEEEEEEEEEECCCCEECCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEEECC
RAGALTGKNALTANVGENVLIVHSQANRDSRPHLIGGHGDYVWETGKFSNAPETGLETWF
CCCEEECCCCEEECCCCCEEEEEECCCCCCCCCEECCCCCEEEECCCCCCCCCCCCEEEE
IRGGSAGAALYKFLQPGIYAYVTHNLIEAADLGATAHFKVEGKWNDDLMTQVRAPADIPT
EECCCCHHHHHHHHCCCEEEEEHHHHHHHHHCCCEEEEEEECCCCHHHHHHHCCCCCCCC
GSTN
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8899992 [H]