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The map label for this gene is appB [H]

Identifier: 146338196

GI number: 146338196

Start: 1164261

End: 1165220

Strand: Direct

Name: appB [H]

Synonym: BRADO1094

Alternate gene names: 146338196

Gene position: 1164261-1165220 (Clockwise)

Preceding gene: 146338195

Following gene: 146338197

Centisome position: 15.61

GC content: 63.44

Gene sequence:

>960_bases
ATGGGAAACTACCTTCTCAGGCGCCTCATCGTGGCCGTGCCGAGCCTGCTCGGCATCAGCCTGATTCTGTTCGTGCTGCT
TGCGCTCGCGCCGGGTGATCCGTTCGGCGAGCTCGCGAGCAATCCGAACATTCCCCCGGAGGTCCGCGAGGCCCTGCGCA
TCAAGTTCGGCATGGATGATCCGATCATGGTGCGCTATTTCCGCTGGCTGCTCGCCATGCTGCATGGCGACTGGGGCTTC
TCCTTCGCGAGCCGCGTCAACGTCGATACGCTGATCCTGCAGCGGATTCCGGCCACGCTCTACGTGGTCGGCACCGCGCA
GCTCTTGGCGCTGATCGTCGCGATCCCGGTCGGCGTGCTGGCGGCGCGCCGGCCCTATTCGATCTTCGACCAGATCGCCA
ACACCTTCGCCTTCATCGGTTTCTCGCTGCCGACCTTCTTCACCGGCCTGCTCCTGATCCTGGTGTTCTCGGTCAATCTC
GGCTGGCTGCCGTTCGTCTACCGCGCCGACATCCCGGCGACGGGGTGGATATGGTGGTGGGAAAATTTCAAGCAGGCGGT
GATGCCGGTCACCGTGCTCGGCCTCTACCAGGCCGCATCCTACACGCGGTATGTCCGGTCGGCCGTGCTCGACGTCATCA
AGCTCGACTACGTCACGACGGCGCGTTCCAAGGGGCTCGACGAGAGCAAGGTGATCGTCAAGCACGTGGTGCGCAATGCG
CTGATCCCGGTGGTGACCCTGGTCGCGCTGCAGATGCCCACGGTGTTCGGCGGCGCCATCGTCACCGAGCAGATCTTCCG
CATTCCCGGCATCGGCTCGCTCCTGATCAGCGCGATCCTCGCCAACGATACGCCCGTCATCATGGCGGTGACCTTCGTGT
TCGCCTGCCTCGTCGTCCTGTTCAACCTCATCGCCGATATTCTGTATGGCTGGCTTGACCCACGCATCTCCTTCCGTTGA

Upstream 100 bases:

>100_bases
AGAGCGGCTGGGACAACGACCTCTGGCAGATCGCCAACTGGTATCGCGAGACCTGATCGCGCCATCAGCATGACCAGCCA
GACATCGTAGAGGTGGAGCG

Downstream 100 bases:

>100_bases
AGCGGCGGTCGCGCCGGCTTCGAAAAAGCGCCGCTTCTCGCCCGGGCGCGATGCGGTCCGACGCTTCCTGCGCCACCGCC
TCGCGGCCGTCAGCATCGTC

Product: oligopeptide transport system permease

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MGNYLLRRLIVAVPSLLGISLILFVLLALAPGDPFGELASNPNIPPEVREALRIKFGMDDPIMVRYFRWLLAMLHGDWGF
SFASRVNVDTLILQRIPATLYVVGTAQLLALIVAIPVGVLAARRPYSIFDQIANTFAFIGFSLPTFFTGLLLILVFSVNL
GWLPFVYRADIPATGWIWWWENFKQAVMPVTVLGLYQAASYTRYVRSAVLDVIKLDYVTTARSKGLDESKVIVKHVVRNA
LIPVVTLVALQMPTVFGGAIVTEQIFRIPGIGSLLISAILANDTPVIMAVTFVFACLVVLFNLIADILYGWLDPRISFR

Sequences:

>Translated_319_residues
MGNYLLRRLIVAVPSLLGISLILFVLLALAPGDPFGELASNPNIPPEVREALRIKFGMDDPIMVRYFRWLLAMLHGDWGF
SFASRVNVDTLILQRIPATLYVVGTAQLLALIVAIPVGVLAARRPYSIFDQIANTFAFIGFSLPTFFTGLLLILVFSVNL
GWLPFVYRADIPATGWIWWWENFKQAVMPVTVLGLYQAASYTRYVRSAVLDVIKLDYVTTARSKGLDESKVIVKHVVRNA
LIPVVTLVALQMPTVFGGAIVTEQIFRIPGIGSLLISAILANDTPVIMAVTFVFACLVVLFNLIADILYGWLDPRISFR
>Mature_318_residues
GNYLLRRLIVAVPSLLGISLILFVLLALAPGDPFGELASNPNIPPEVREALRIKFGMDDPIMVRYFRWLLAMLHGDWGFS
FASRVNVDTLILQRIPATLYVVGTAQLLALIVAIPVGVLAARRPYSIFDQIANTFAFIGFSLPTFFTGLLLILVFSVNLG
WLPFVYRADIPATGWIWWWENFKQAVMPVTVLGLYQAASYTRYVRSAVLDVIKLDYVTTARSKGLDESKVIVKHVVRNAL
IPVVTLVALQMPTVFGGAIVTEQIFRIPGIGSLLISAILANDTPVIMAVTFVFACLVVLFNLIADILYGWLDPRISFR

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence, though, unlike OPP, is incapable of transportin

COG id: COG0601

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789965, Length=338, Percent_Identity=38.7573964497041, Blast_Score=218, Evalue=3e-58,
Organism=Escherichia coli, GI1787053, Length=319, Percent_Identity=36.0501567398119, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1789888, Length=318, Percent_Identity=36.4779874213836, Blast_Score=198, Evalue=5e-52,
Organism=Escherichia coli, GI1787497, Length=319, Percent_Identity=31.974921630094, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1788504, Length=364, Percent_Identity=29.6703296703297, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1787550, Length=265, Percent_Identity=32.8301886792453, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1787761, Length=318, Percent_Identity=31.1320754716981, Blast_Score=128, Evalue=4e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 35401; Mature: 35269

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNYLLRRLIVAVPSLLGISLILFVLLALAPGDPFGELASNPNIPPEVREALRIKFGMDD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCHHHHHHHHHHCCCCC
PIMVRYFRWLLAMLHGDWGFSFASRVNVDTLILQRIPATLYVVGTAQLLALIVAIPVGVL
HHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHCCHHEEEHHHHHHHHHHHHHHHHHH
AARRPYSIFDQIANTFAFIGFSLPTFFTGLLLILVFSVNLGWLPFVYRADIPATGWIWWW
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHEECCCCCCCHHHHH
ENFKQAVMPVTVLGLYQAASYTRYVRSAVLDVIKLDYVTTARSKGLDESKVIVKHVVRNA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LIPVVTLVALQMPTVFGGAIVTEQIFRIPGIGSLLISAILANDTPVIMAVTFVFACLVVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
FNLIADILYGWLDPRISFR
HHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
GNYLLRRLIVAVPSLLGISLILFVLLALAPGDPFGELASNPNIPPEVREALRIKFGMDD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCHHHHHHHHHHCCCCC
PIMVRYFRWLLAMLHGDWGFSFASRVNVDTLILQRIPATLYVVGTAQLLALIVAIPVGVL
HHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHCCHHEEEHHHHHHHHHHHHHHHHHH
AARRPYSIFDQIANTFAFIGFSLPTFFTGLLLILVFSVNLGWLPFVYRADIPATGWIWWW
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHEECCCCCCCHHHHH
ENFKQAVMPVTVLGLYQAASYTRYVRSAVLDVIKLDYVTTARSKGLDESKVIVKHVVRNA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LIPVVTLVALQMPTVFGGAIVTEQIFRIPGIGSLLISAILANDTPVIMAVTFVFACLVVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
FNLIADILYGWLDPRISFR
HHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]