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The map label for this gene is appA [H]

Identifier: 146338195

GI number: 146338195

Start: 1162423

End: 1164216

Strand: Direct

Name: appA [H]

Synonym: BRADO1093

Alternate gene names: 146338195

Gene position: 1162423-1164216 (Clockwise)

Preceding gene: 146338194

Following gene: 146338196

Centisome position: 15.59

GC content: 62.32

Gene sequence:

>1794_bases
ATGAACGAGCGTGACCTCCGTAACCAGATCGCTGATGTCAAAGCGGGGCGCCTGTCGCGGCGCGATTTTGTGCAGCGGAT
GATGGCTGTGGGGCTGACGGCGCCGATGGCCGGCACGATGCTCGCGCATTCCGGCGTCGCCTTCGCGGCGGACTCGATGG
AGTACAAGCCGACCAAGGCCGGCGGCGGTGGTCCGCTCAAGCTCTTGTTCTGGCAGGCGCCGACGCTGCTCAATCCACAC
TTCGCCAACGGCACCAAGGATCAGGAAGCCTGCCGGCTGTTCTACGAGCCGCTCGCCGGCTGGGACGCCGACGGCAATCT
CGTGCCGATCCTCGCCGCGGAGATTCCGAGCAAGGAGAACGGCCTGCTCGGCGCCGACGGTCTCTCCGTCACCTGGAAGC
TGAAGCAGGGCGTGAAGTGGCACGACGGCAAGCCGTTCACCGCCGACGACGTCATCTTCAACTGGGAATACTCGGTCAAT
CCCGACACCGCCGCGGTCTCGGTCGGCAGCTATCGCGGTATCAAGTCGATCGACAAGATCGACGATTTCACCGTGAAGCT
GAACTTCAAGGCGCCGACGCCGTTCTGGGCCGATGCCTTCGTTGCAGCCTACGGTCTCGTCATTCCGAAGCACCTGTTCG
CTGATTATATCGGCGGCAAGTCGCGCGAGGCGCCGGCCAATCTGAAGCCGGTGGGCACCGGCCCGTACATCTATGTCGAC
TTCAAGCCGGGCGATTCGATCTCCGCCAAGCGCAACCCCGACTACCATCAGGCGAACCGGCCGTATTTCGACTCGGTCGA
GGTCAAGGGCGGCGGCGATGCCGTGTCGGCGGCCCGCGCGGTGCTGCAGACCGGCGAATACGACTACGCCTACAATCTCC
AGGTCGAGGACGAGATCCTCACCAAGCTGGAAGCCGGCGGCCAGGGCAAGGCCCAGCTCACGGTCACCGGCCACATCGAG
TACATCGCGCTCAACGTCACCGATCCCTGGACCGAGGTCGATGGCGAGCGCGCGAGCGTCAAGACCAAGCATCCGTTCTT
CACTGATCCGGCGGTGCGCAAGGCGATCAACCTCCTGATCGACCGTGCCTCGGTGCAGAAGTTCATCTATGGCCGCGCCG
GCGTCGCGACCGCGAACTTCCTCAACAACCCCGAGCGCTTCCGCTCGCCGAACAACAAGTTCGAATTCTCGATCGACAAG
GCCAACCAGATCCTGGAGGAGGCCGGCTGGAAGAAGAACGCATCAGGCATCCGCGAGAAGGACGGCAAGCCGATCAAGCT
GGTGTTCCAGACCTCGATCAACGCGCCGCGCCAGAAGAACCAGGCGATCATCAAGCAGGCGTTCCAGAAAGCCGGTATCG
AGGTCGAGCTGAAATCGGTGGTCGCCTCGGTGTTCTTCTCGTCGGATCCGGCGAACCCCGACACCTACCCGCACTTCTAT
TGCGACATGGAGATGTATCAGACCACCATGCCGCAGGCCGACCCGCAGTTCTTCATGAACCAGTTCACCTCGTGGGAGGT
CGCCAGCAAGGAGAACAAGTGGCAGGGCCGCAACATCTCGCGCTGGCAAAATAAGGAGTATGACGAGACCTACAAGCAGG
CATCGAGCGAGCTCGACCCGGTCAAGCGCGCGGCATTGTTCGTCAAGCTCAACGACCTCGTGGTGCAGGACAACTACATC
CTGCCCGAGATCAACCGGCGCAACTGCATCGGTCTGAAGAACGGCATGGTCGTGAACAAGAGCGGCTGGGACAACGACCT
CTGGCAGATCGCCAACTGGTATCGCGAGACCTGA

Upstream 100 bases:

>100_bases
TTGACAGTCGCTCAGAAGGCTGATCCAATTTTGTGCAGCTCCGCGAAGATGCTTGGCGGGGCGCTTTTTTTGGCATTCAG
CACCACACTGGAGTCGATGA

Downstream 100 bases:

>100_bases
TCGCGCCATCAGCATGACCAGCCAGACATCGTAGAGGTGGAGCGATGGGAAACTACCTTCTCAGGCGCCTCATCGTGGCC
GTGCCGAGCCTGCTCGGCAT

Product: oligopeptide ABC transporter periplasmic substrate-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 597; Mature: 597

Protein sequence:

>597_residues
MNERDLRNQIADVKAGRLSRRDFVQRMMAVGLTAPMAGTMLAHSGVAFAADSMEYKPTKAGGGGPLKLLFWQAPTLLNPH
FANGTKDQEACRLFYEPLAGWDADGNLVPILAAEIPSKENGLLGADGLSVTWKLKQGVKWHDGKPFTADDVIFNWEYSVN
PDTAAVSVGSYRGIKSIDKIDDFTVKLNFKAPTPFWADAFVAAYGLVIPKHLFADYIGGKSREAPANLKPVGTGPYIYVD
FKPGDSISAKRNPDYHQANRPYFDSVEVKGGGDAVSAARAVLQTGEYDYAYNLQVEDEILTKLEAGGQGKAQLTVTGHIE
YIALNVTDPWTEVDGERASVKTKHPFFTDPAVRKAINLLIDRASVQKFIYGRAGVATANFLNNPERFRSPNNKFEFSIDK
ANQILEEAGWKKNASGIREKDGKPIKLVFQTSINAPRQKNQAIIKQAFQKAGIEVELKSVVASVFFSSDPANPDTYPHFY
CDMEMYQTTMPQADPQFFMNQFTSWEVASKENKWQGRNISRWQNKEYDETYKQASSELDPVKRAALFVKLNDLVVQDNYI
LPEINRRNCIGLKNGMVVNKSGWDNDLWQIANWYRET

Sequences:

>Translated_597_residues
MNERDLRNQIADVKAGRLSRRDFVQRMMAVGLTAPMAGTMLAHSGVAFAADSMEYKPTKAGGGGPLKLLFWQAPTLLNPH
FANGTKDQEACRLFYEPLAGWDADGNLVPILAAEIPSKENGLLGADGLSVTWKLKQGVKWHDGKPFTADDVIFNWEYSVN
PDTAAVSVGSYRGIKSIDKIDDFTVKLNFKAPTPFWADAFVAAYGLVIPKHLFADYIGGKSREAPANLKPVGTGPYIYVD
FKPGDSISAKRNPDYHQANRPYFDSVEVKGGGDAVSAARAVLQTGEYDYAYNLQVEDEILTKLEAGGQGKAQLTVTGHIE
YIALNVTDPWTEVDGERASVKTKHPFFTDPAVRKAINLLIDRASVQKFIYGRAGVATANFLNNPERFRSPNNKFEFSIDK
ANQILEEAGWKKNASGIREKDGKPIKLVFQTSINAPRQKNQAIIKQAFQKAGIEVELKSVVASVFFSSDPANPDTYPHFY
CDMEMYQTTMPQADPQFFMNQFTSWEVASKENKWQGRNISRWQNKEYDETYKQASSELDPVKRAALFVKLNDLVVQDNYI
LPEINRRNCIGLKNGMVVNKSGWDNDLWQIANWYRET
>Mature_597_residues
MNERDLRNQIADVKAGRLSRRDFVQRMMAVGLTAPMAGTMLAHSGVAFAADSMEYKPTKAGGGGPLKLLFWQAPTLLNPH
FANGTKDQEACRLFYEPLAGWDADGNLVPILAAEIPSKENGLLGADGLSVTWKLKQGVKWHDGKPFTADDVIFNWEYSVN
PDTAAVSVGSYRGIKSIDKIDDFTVKLNFKAPTPFWADAFVAAYGLVIPKHLFADYIGGKSREAPANLKPVGTGPYIYVD
FKPGDSISAKRNPDYHQANRPYFDSVEVKGGGDAVSAARAVLQTGEYDYAYNLQVEDEILTKLEAGGQGKAQLTVTGHIE
YIALNVTDPWTEVDGERASVKTKHPFFTDPAVRKAINLLIDRASVQKFIYGRAGVATANFLNNPERFRSPNNKFEFSIDK
ANQILEEAGWKKNASGIREKDGKPIKLVFQTSINAPRQKNQAIIKQAFQKAGIEVELKSVVASVFFSSDPANPDTYPHFY
CDMEMYQTTMPQADPQFFMNQFTSWEVASKENKWQGRNISRWQNKEYDETYKQASSELDPVKRAALFVKLNDLVVQDNYI
LPEINRRNCIGLKNGMVVNKSGWDNDLWQIANWYRET

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=506, Percent_Identity=23.1225296442688, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1789397, Length=514, Percent_Identity=21.2062256809339, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1787495, Length=514, Percent_Identity=22.9571984435798, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1789966, Length=523, Percent_Identity=24.8565965583174, Blast_Score=74, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 66691; Mature: 66691

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNERDLRNQIADVKAGRLSRRDFVQRMMAVGLTAPMAGTMLAHSGVAFAADSMEYKPTKA
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEECCCCCCCCCC
GGGGPLKLLFWQAPTLLNPHFANGTKDQEACRLFYEPLAGWDADGNLVPILAAEIPSKEN
CCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCC
GLLGADGLSVTWKLKQGVKWHDGKPFTADDVIFNWEYSVNPDTAAVSVGSYRGIKSIDKI
CEEECCCCEEEEEECCCCEECCCCCCCCCCEEEEEEEECCCCCEEEECCCCCCCCHHHCC
DDFTVKLNFKAPTPFWADAFVAAYGLVIPKHLFADYIGGKSREAPANLKPVGTGPYIYVD
CCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCEEEEE
FKPGDSISAKRNPDYHQANRPYFDSVEVKGGGDAVSAARAVLQTGEYDYAYNLQVEDEIL
ECCCCCCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHCCCCCEEEEEEEHHHHH
TKLEAGGQGKAQLTVTGHIEYIALNVTDPWTEVDGERASVKTKHPFFTDPAVRKAINLLI
HHHHCCCCCCEEEEEEEEEEEEEEECCCCHHHCCCCCCCEEECCCCCCCHHHHHHHHHHH
DRASVQKFIYGRAGVATANFLNNPERFRSPNNKFEFSIDKANQILEEAGWKKNASGIREK
HHHHHHHHHHCCCCCEEHHHCCCHHHHCCCCCCEEEEEHHHHHHHHHCCCCCCCCCCHHC
DGKPIKLVFQTSINAPRQKNQAIIKQAFQKAGIEVELKSVVASVFFSSDPANPDTYPHFY
CCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHCCCCCCCCCCCEEE
CDMEMYQTTMPQADPQFFMNQFTSWEVASKENKWQGRNISRWQNKEYDETYKQASSELDP
ECHHHHHHCCCCCCHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCH
VKRAALFVKLNDLVVQDNYILPEINRRNCIGLKNGMVVNKSGWDNDLWQIANWYRET
HHHEEEEEEEEEEEEECCEECCCCCCCCEEECCCCEEEECCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MNERDLRNQIADVKAGRLSRRDFVQRMMAVGLTAPMAGTMLAHSGVAFAADSMEYKPTKA
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEECCCCCCCCCC
GGGGPLKLLFWQAPTLLNPHFANGTKDQEACRLFYEPLAGWDADGNLVPILAAEIPSKEN
CCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCC
GLLGADGLSVTWKLKQGVKWHDGKPFTADDVIFNWEYSVNPDTAAVSVGSYRGIKSIDKI
CEEECCCCEEEEEECCCCEECCCCCCCCCCEEEEEEEECCCCCEEEECCCCCCCCHHHCC
DDFTVKLNFKAPTPFWADAFVAAYGLVIPKHLFADYIGGKSREAPANLKPVGTGPYIYVD
CCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCEEEEE
FKPGDSISAKRNPDYHQANRPYFDSVEVKGGGDAVSAARAVLQTGEYDYAYNLQVEDEIL
ECCCCCCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHCCCCCEEEEEEEHHHHH
TKLEAGGQGKAQLTVTGHIEYIALNVTDPWTEVDGERASVKTKHPFFTDPAVRKAINLLI
HHHHCCCCCCEEEEEEEEEEEEEEECCCCHHHCCCCCCCEEECCCCCCCHHHHHHHHHHH
DRASVQKFIYGRAGVATANFLNNPERFRSPNNKFEFSIDKANQILEEAGWKKNASGIREK
HHHHHHHHHHCCCCCEEHHHCCCHHHHCCCCCCEEEEEHHHHHHHHHCCCCCCCCCCHHC
DGKPIKLVFQTSINAPRQKNQAIIKQAFQKAGIEVELKSVVASVFFSSDPANPDTYPHFY
CCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHCCCCCCCCCCCEEE
CDMEMYQTTMPQADPQFFMNQFTSWEVASKENKWQGRNISRWQNKEYDETYKQASSELDP
ECHHHHHHCCCCCCHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCH
VKRAALFVKLNDLVVQDNYILPEINRRNCIGLKNGMVVNKSGWDNDLWQIANWYRET
HHHEEEEEEEEEEEEECCEECCCCCCCCEEECCCCEEEECCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]