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The map label for this gene is appA [H]

Identifier: 146338194

GI number: 146338194

Start: 1160451

End: 1162166

Strand: Direct

Name: appA [H]

Synonym: BRADO1092

Alternate gene names: 146338194

Gene position: 1160451-1162166 (Clockwise)

Preceding gene: 146338193

Following gene: 146338195

Centisome position: 15.56

GC content: 63.11

Gene sequence:

>1716_bases
GTGCTCGCGGCCGGGCTGACGGCACCGCTGGCCGGCATGATGCTGGCGCATTCAGGGGTGGCGATGGCCGCGCAGGCGTT
TCCGTACAAGCCGACCAAGGCCGGCGGCGGCGGTCCGCTGCGCATCCTGTTCTGGCAGGCGCCGACCCTGCTCAATCCGC
ATTTCGCGATCGGCTCCAAGGACCAGGAAGCCTCGCGCGCCTTCTACGAGCCGCTGGCGGGATGGGATGCCGACGGCAAT
CTCGTGCCGGTGCTCGCCGCGGAAATCCCAACTCTCGCGAACGGCACGCTTGCGGCGGACGGCACCTCTGTCGTCTGGAA
GCTGAAGCCGGGGGTGAAATGGCATGACGGCCAGCCTTTCACGGCCGACGACGTGATCTTCAACTGGCAGTACGCGACAC
ATCCCGAGACCTCGGCGGTCACCATCGCCGACTACAAGGACATCAAGACGATCGAGAGGATCGACGACCTTACGGTTCGC
CTCGTATTCGCAAAGCCGACGCCGCAATGGTTCACGCCGTTCGTCAGCGGCCGTGGCGCGATCATCCCGAAACATTTGTT
CGCCGACTATATCGGCGCCAAGTCCCGCGAGGCGCCGAACAACCTCAAACCGGTCGGTACCGGCGCCTATCTGTTCGTCG
ACTTCAAGCCCGGCGACATGCTCACGGGCAAGCTCAATCCCGACTACCACGTGCCCAACCGTCCGTATTTCGACACGCTG
GAGATCAAGGGCGGCGGCGATGCGGTGTCGGCGGCGCGCGCGGTGCTGCAGACCGGCGAATTCGACTACGCCTGGAATCT
GCAGGTCGAGGACGAGATCCTCGTCAAGCTGGAGAGCGGCGGGCCAGGCGCGGTCGAGATCGTGCCAACCGGCAATCTGG
AATTCATCCTGCTCAACGCCACGGATCCCTGGACCGAGGTCGACGGCGAGCGCGCGAGCGTCAAGACCGGGCATCCCTTG
TTCTCGGATCCCGCGGTGCGCGAGGCGATCAACCTGCTGATCGACCGCGCTTCGATTCAGAAGTTCATCTATGGTCGCGG
CGGCATCGCCACGGCGAACGCCGTCAACAATCCCGAGCGCTATCGCTCGCCCAACATGCGTTTCGAGTTCTCGGTCGAGA
AGGCCAACCAGATCCTGGAGAACGCCGGCTGGAAGAAGGGCGCGAACGGCATCCGCGAGAAGGACGGCAAGCCGCTGAAG
ATCGTATTCCAGACCTCGACCAATGGGCCACGCCAGAAGAACCAGGCGATCATCAAGCAGGCCTGCCAGAAAGCCGGCAT
CGAGATGGAGTTGAAATCGGTGGTCGGCTCCGTGTTCTTCTCCAGCGACGTCGCCAATCCCGACACCTATGCGCATTTTT
ACTGCGACATGGAAATGTATCAGATGACGATGGCCTCGCCCGAGCCGCAGGCCTTCATGAGCCAGTACGTATCGTGGGAA
GCGGCGAGCAAGGCCAACAAATGGCAGGGCCGCAACATCGCGCGCTGGCAGAACAAGGCGTATGACGATCTCTACAACGC
GCTGACGGTCGAGCTTGATCCGGTCAAGCGCGCAGCGCTCTACATCAAGCTCAACGATATCGTCTGCTCGGAACGCCACG
TGCTTCCGGAGCTGAACCGGCCGCGCATCTCCGGCATCCGCAAGGGGCTGCAGACGCACTCCTCGGGCTGGGACAACGAC
CTCTGGCAATTGCCGAACTGGTATCGCGAGAGCTGA

Upstream 100 bases:

>100_bases
ACAATTTGGAGGAGAGACAGTCATGGACGAACAGGACCTGCGCCGCGAGATCGCTGCGGTCAAAGAAGGGCGGCTGTCAC
GCCGCGATTTCGTTCAGCGG

Downstream 100 bases:

>100_bases
GCACATTCGCTGGGCTGTATCCGAACACCCCGGCTGTGGCTGCCACCATACCGGGGTGAGTTCCAGTTTCCGGAATTCCG
GAGGGCTGCTCTTGCTGCAG

Product: oligopeptide ABC transporter periplasmic substrate-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 571; Mature: 571

Protein sequence:

>571_residues
MLAAGLTAPLAGMMLAHSGVAMAAQAFPYKPTKAGGGGPLRILFWQAPTLLNPHFAIGSKDQEASRAFYEPLAGWDADGN
LVPVLAAEIPTLANGTLAADGTSVVWKLKPGVKWHDGQPFTADDVIFNWQYATHPETSAVTIADYKDIKTIERIDDLTVR
LVFAKPTPQWFTPFVSGRGAIIPKHLFADYIGAKSREAPNNLKPVGTGAYLFVDFKPGDMLTGKLNPDYHVPNRPYFDTL
EIKGGGDAVSAARAVLQTGEFDYAWNLQVEDEILVKLESGGPGAVEIVPTGNLEFILLNATDPWTEVDGERASVKTGHPL
FSDPAVREAINLLIDRASIQKFIYGRGGIATANAVNNPERYRSPNMRFEFSVEKANQILENAGWKKGANGIREKDGKPLK
IVFQTSTNGPRQKNQAIIKQACQKAGIEMELKSVVGSVFFSSDVANPDTYAHFYCDMEMYQMTMASPEPQAFMSQYVSWE
AASKANKWQGRNIARWQNKAYDDLYNALTVELDPVKRAALYIKLNDIVCSERHVLPELNRPRISGIRKGLQTHSSGWDND
LWQLPNWYRES

Sequences:

>Translated_571_residues
MLAAGLTAPLAGMMLAHSGVAMAAQAFPYKPTKAGGGGPLRILFWQAPTLLNPHFAIGSKDQEASRAFYEPLAGWDADGN
LVPVLAAEIPTLANGTLAADGTSVVWKLKPGVKWHDGQPFTADDVIFNWQYATHPETSAVTIADYKDIKTIERIDDLTVR
LVFAKPTPQWFTPFVSGRGAIIPKHLFADYIGAKSREAPNNLKPVGTGAYLFVDFKPGDMLTGKLNPDYHVPNRPYFDTL
EIKGGGDAVSAARAVLQTGEFDYAWNLQVEDEILVKLESGGPGAVEIVPTGNLEFILLNATDPWTEVDGERASVKTGHPL
FSDPAVREAINLLIDRASIQKFIYGRGGIATANAVNNPERYRSPNMRFEFSVEKANQILENAGWKKGANGIREKDGKPLK
IVFQTSTNGPRQKNQAIIKQACQKAGIEMELKSVVGSVFFSSDVANPDTYAHFYCDMEMYQMTMASPEPQAFMSQYVSWE
AASKANKWQGRNIARWQNKAYDDLYNALTVELDPVKRAALYIKLNDIVCSERHVLPELNRPRISGIRKGLQTHSSGWDND
LWQLPNWYRES
>Mature_571_residues
MLAAGLTAPLAGMMLAHSGVAMAAQAFPYKPTKAGGGGPLRILFWQAPTLLNPHFAIGSKDQEASRAFYEPLAGWDADGN
LVPVLAAEIPTLANGTLAADGTSVVWKLKPGVKWHDGQPFTADDVIFNWQYATHPETSAVTIADYKDIKTIERIDDLTVR
LVFAKPTPQWFTPFVSGRGAIIPKHLFADYIGAKSREAPNNLKPVGTGAYLFVDFKPGDMLTGKLNPDYHVPNRPYFDTL
EIKGGGDAVSAARAVLQTGEFDYAWNLQVEDEILVKLESGGPGAVEIVPTGNLEFILLNATDPWTEVDGERASVKTGHPL
FSDPAVREAINLLIDRASIQKFIYGRGGIATANAVNNPERYRSPNMRFEFSVEKANQILENAGWKKGANGIREKDGKPLK
IVFQTSTNGPRQKNQAIIKQACQKAGIEMELKSVVGSVFFSSDVANPDTYAHFYCDMEMYQMTMASPEPQAFMSQYVSWE
AASKANKWQGRNIARWQNKAYDDLYNALTVELDPVKRAALYIKLNDIVCSERHVLPELNRPRISGIRKGLQTHSSGWDND
LWQLPNWYRES

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789397, Length=531, Percent_Identity=21.6572504708098, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1787052, Length=515, Percent_Identity=23.1067961165049, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1787495, Length=512, Percent_Identity=21.6796875, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 63051; Mature: 63051

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAAGLTAPLAGMMLAHSGVAMAAQAFPYKPTKAGGGGPLRILFWQAPTLLNPHFAIGSK
CCCCCCHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCEECCCC
DQEASRAFYEPLAGWDADGNLVPVLAAEIPTLANGTLAADGTSVVWKLKPGVKWHDGQPF
CHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCEEECCCCEEEEEECCCCEECCCCCC
TADDVIFNWQYATHPETSAVTIADYKDIKTIERIDDLTVRLVFAKPTPQWFTPFVSGRGA
CCCCEEEEEEECCCCCCCEEEEECCHHHHHHHHHCCEEEEEEEECCCCHHHHHHHCCCCC
IIPKHLFADYIGAKSREAPNNLKPVGTGAYLFVDFKPGDMLTGKLNPDYHVPNRPYFDTL
CCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEECCCCCCCCCCCCCEEEE
EIKGGGDAVSAARAVLQTGEFDYAWNLQVEDEILVKLESGGPGAVEIVPTGNLEFILLNA
EECCCCCHHHHHHHHHHCCCCCEEEEEEECCEEEEEEECCCCCEEEEEECCCEEEEEEEC
TDPWTEVDGERASVKTGHPLFSDPAVREAINLLIDRASIQKFIYGRGGIATANAVNNPER
CCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHH
YRSPNMRFEFSVEKANQILENAGWKKGANGIREKDGKPLKIVFQTSTNGPRQKNQAIIKQ
HCCCCCEEEEEHHHHHHHHHHCCCCCCCCCCHHCCCCCEEEEEEECCCCCCHHHHHHHHH
ACQKAGIEMELKSVVGSVFFSSDVANPDTYAHFYCDMEMYQMTMASPEPQAFMSQYVSWE
HHHHCCCCEEHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHHHCCCCHHHHHHHHHHHH
AASKANKWQGRNIARWQNKAYDDLYNALTVELDPVKRAALYIKLNDIVCSERHVLPELNR
HHHCCCCCCCCCHHHHHHHHHHHHHHEEEEEECCCCEEEEEEEEECEEECCCCCCCCCCC
PRISGIRKGLQTHSSGWDNDLWQLPNWYRES
CHHHHHHHHHHHCCCCCCCCHHHCCHHHCCC
>Mature Secondary Structure
MLAAGLTAPLAGMMLAHSGVAMAAQAFPYKPTKAGGGGPLRILFWQAPTLLNPHFAIGSK
CCCCCCHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCEECCCC
DQEASRAFYEPLAGWDADGNLVPVLAAEIPTLANGTLAADGTSVVWKLKPGVKWHDGQPF
CHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCEEECCCCEEEEEECCCCEECCCCCC
TADDVIFNWQYATHPETSAVTIADYKDIKTIERIDDLTVRLVFAKPTPQWFTPFVSGRGA
CCCCEEEEEEECCCCCCCEEEEECCHHHHHHHHHCCEEEEEEEECCCCHHHHHHHCCCCC
IIPKHLFADYIGAKSREAPNNLKPVGTGAYLFVDFKPGDMLTGKLNPDYHVPNRPYFDTL
CCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEECCCCCCCCCCCCCEEEE
EIKGGGDAVSAARAVLQTGEFDYAWNLQVEDEILVKLESGGPGAVEIVPTGNLEFILLNA
EECCCCCHHHHHHHHHHCCCCCEEEEEEECCEEEEEEECCCCCEEEEEECCCEEEEEEEC
TDPWTEVDGERASVKTGHPLFSDPAVREAINLLIDRASIQKFIYGRGGIATANAVNNPER
CCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHH
YRSPNMRFEFSVEKANQILENAGWKKGANGIREKDGKPLKIVFQTSTNGPRQKNQAIIKQ
HCCCCCEEEEEHHHHHHHHHHCCCCCCCCCCHHCCCCCEEEEEEECCCCCCHHHHHHHHH
ACQKAGIEMELKSVVGSVFFSSDVANPDTYAHFYCDMEMYQMTMASPEPQAFMSQYVSWE
HHHHCCCCEEHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHHHCCCCHHHHHHHHHHHH
AASKANKWQGRNIARWQNKAYDDLYNALTVELDPVKRAALYIKLNDIVCSERHVLPELNR
HHHCCCCCCCCCHHHHHHHHHHHHHHEEEEEECCCCEEEEEEEEECEEECCCCCCCCCCC
PRISGIRKGLQTHSSGWDNDLWQLPNWYRES
CHHHHHHHHHHHCCCCCCCCHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]