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The map label for this gene is mltB [H]

Identifier: 146338190

GI number: 146338190

Start: 1155776

End: 1157203

Strand: Reverse

Name: mltB [H]

Synonym: BRADO1088

Alternate gene names: 146338190

Gene position: 1157203-1155776 (Counterclockwise)

Preceding gene: 146338192

Following gene: 146338189

Centisome position: 15.52

GC content: 68.0

Gene sequence:

>1428_bases
GTGGCCCACCCTGAGATGGACCTATTGAAGATGCGACGCAACGTCATGCTGGCAGCCTTGAGGACCGGAATGCGGATGGC
GGTGGTGGCCGCGGCCCTGGCGACGGTGTCCGGTTCCGCCAACGCGCAATCCGGCAATCCGCTGAACTTCCTCGACAGCC
TGTTCACGGGTACCCTTTCGAAGGACAATCCCGGCGCCGCCGCGCCCGCGCAGGCGCCCTCCGTCTCGGGGCCGCAGCCC
TGGAGCGGCGAGGACGGTGCCTCCGGCCATCCGCTGATGACGGCGGCGGCGATCCGCGAGGCCGCCGGCAATTTCGACGC
CTGCGTCGCAGGCATGTGGCCAGATGCCGCACGGCGCAACATCACGCAGGACAATTTCCAGCGCTTCACGGCGGGCCTCA
CGCCCGACCTGCGCATCATGGATCTGCTCGACGCCCAGCCGGAGTTCACCAAGTCGATCTGGGACTATCTCGACATCCTG
GTGAACGACAATCGCCTGGCCAAGGGCCGCGAGGTGCTGGCCAAATACAAGCCGCAGTTCGACGCCACCGAGCGCGCCTA
TGGCGTCGACCGCTTCATTATCGCGGCGATCTGGGGCATCGAATCCAACTATTCGACCCAGATCGGCGACCGCAGCGTGC
TGCAGTCGACCGCGACGCTCGCCTGCATCGGCCGCCGCCAAGCCTATTTCAAGGATGAGTTCCTGTCGGCCCTGGAGATC
CTCAACCGCGGCGATCTCAGGCCCGAACAGTTGCGCGGCTCCTGGGCCGGCGCCTTCGGCCCGACCCAGTTCATGCCGAC
TGCCTTCAAGCGCTTCGCCGTGGACGCCGACGGCGACGGCCGCCGCGACGTCGTCGACAATCCCGCCGACCTGATCGCCT
CGACCGCCAACAATCTGAAGAAGGACGGCTGGCAGAGCGGCCAGAGCTGGGGCTACGAGGTCGTGCTGCCGCAGGGCTTC
AACTTCATGCTGGCCGACCGCGCCAAGACCATGAGCTTCGCCCAATGGGAACAGCTCGGCCTGCGCCGCGCCAACGGCCA
GCCGTTCCCGGCGACCACCGAGAAGGCCTATCTGCTGGCGCCGGCGGGCGCCCAGGGCCCGGGTTTCCTGATGCTGCAGA
ATTTCCGCGTCATCATGAAATACAATCCGGCCGAGGCCTATGCGCTGGCGATCGGCCACTTCGCCGACCGGCTGCGCGGC
GCCCCGCCCTTCGTGCAGGCCTGGCCGCGCGAGGAGCGCACCCTGTCCCGGGCGGAGCGGCTGGAGCTGCAACAATTGCT
GGCGAACCGCGGATTCTACCGCGGAACGCCGGACGGCCAGTTCGGCGGCCAGACCCGCGAGGCGCTGCGGAACTTCCAGG
CCTCGATCGGGGCGCCGGCGGACGGTTTTGCCACCACGGACGTGCTGGAACGGCTGCGTGGCCGCTGA

Upstream 100 bases:

>100_bases
GGTCGACTGGTTCCGTTCGAGGCGCGATGGCACGGCCATATTTCGCTGCTGAGAAACGATTAACCTTGTTAATCTCCCGT
TAAGCAGGTCCGGGCCTCCT

Downstream 100 bases:

>100_bases
TCGCCCGCGATGCGCCGGCGCGCGGTAACCTTCCACGGCAAATACCGCGACCCGTCAACACCAACAGGTGAATGCCCGTT
TGCGGCCTGATTCCGGTCCT

Product: putative membrane-bound lytic murein transglycosylase signal peptide

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MAHPEMDLLKMRRNVMLAALRTGMRMAVVAAALATVSGSANAQSGNPLNFLDSLFTGTLSKDNPGAAAPAQAPSVSGPQP
WSGEDGASGHPLMTAAAIREAAGNFDACVAGMWPDAARRNITQDNFQRFTAGLTPDLRIMDLLDAQPEFTKSIWDYLDIL
VNDNRLAKGREVLAKYKPQFDATERAYGVDRFIIAAIWGIESNYSTQIGDRSVLQSTATLACIGRRQAYFKDEFLSALEI
LNRGDLRPEQLRGSWAGAFGPTQFMPTAFKRFAVDADGDGRRDVVDNPADLIASTANNLKKDGWQSGQSWGYEVVLPQGF
NFMLADRAKTMSFAQWEQLGLRRANGQPFPATTEKAYLLAPAGAQGPGFLMLQNFRVIMKYNPAEAYALAIGHFADRLRG
APPFVQAWPREERTLSRAERLELQQLLANRGFYRGTPDGQFGGQTREALRNFQASIGAPADGFATTDVLERLRGR

Sequences:

>Translated_475_residues
MAHPEMDLLKMRRNVMLAALRTGMRMAVVAAALATVSGSANAQSGNPLNFLDSLFTGTLSKDNPGAAAPAQAPSVSGPQP
WSGEDGASGHPLMTAAAIREAAGNFDACVAGMWPDAARRNITQDNFQRFTAGLTPDLRIMDLLDAQPEFTKSIWDYLDIL
VNDNRLAKGREVLAKYKPQFDATERAYGVDRFIIAAIWGIESNYSTQIGDRSVLQSTATLACIGRRQAYFKDEFLSALEI
LNRGDLRPEQLRGSWAGAFGPTQFMPTAFKRFAVDADGDGRRDVVDNPADLIASTANNLKKDGWQSGQSWGYEVVLPQGF
NFMLADRAKTMSFAQWEQLGLRRANGQPFPATTEKAYLLAPAGAQGPGFLMLQNFRVIMKYNPAEAYALAIGHFADRLRG
APPFVQAWPREERTLSRAERLELQQLLANRGFYRGTPDGQFGGQTREALRNFQASIGAPADGFATTDVLERLRGR
>Mature_474_residues
AHPEMDLLKMRRNVMLAALRTGMRMAVVAAALATVSGSANAQSGNPLNFLDSLFTGTLSKDNPGAAAPAQAPSVSGPQPW
SGEDGASGHPLMTAAAIREAAGNFDACVAGMWPDAARRNITQDNFQRFTAGLTPDLRIMDLLDAQPEFTKSIWDYLDILV
NDNRLAKGREVLAKYKPQFDATERAYGVDRFIIAAIWGIESNYSTQIGDRSVLQSTATLACIGRRQAYFKDEFLSALEIL
NRGDLRPEQLRGSWAGAFGPTQFMPTAFKRFAVDADGDGRRDVVDNPADLIASTANNLKKDGWQSGQSWGYEVVLPQGFN
FMLADRAKTMSFAQWEQLGLRRANGQPFPATTEKAYLLAPAGAQGPGFLMLQNFRVIMKYNPAEAYALAIGHFADRLRGA
PPFVQAWPREERTLSRAERLELQQLLANRGFYRGTPDGQFGGQTREALRNFQASIGAPADGFATTDVLERLRGR

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=242, Percent_Identity=30.5785123966942, Blast_Score=95, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 52008; Mature: 51877

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
AHPEMDLLKMRRNVMLAALRTGMRMAVVAAALATVSGSANAQSGNPLNFLDSLFTGTLS
CCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCC
KDNPGAAAPAQAPSVSGPQPWSGEDGASGHPLMTAAAIREAAGNFDACVAGMWPDAARRN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHCCCCCHHHHCC
ITQDNFQRFTAGLTPDLRIMDLLDAQPEFTKSIWDYLDILVNDNRLAKGREVLAKYKPQF
CCHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCC
DATERAYGVDRFIIAAIWGIESNYSTQIGDRSVLQSTATLACIGRRQAYFKDEFLSALEI
CCHHHHHCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNRGDLRPEQLRGSWAGAFGPTQFMPTAFKRFAVDADGDGRRDVVDNPADLIASTANNLK
HHCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHH
KDGWQSGQSWGYEVVLPQGFNFMLADRAKTMSFAQWEQLGLRRANGQPFPATTEKAYLLA
HHCCCCCCCCCEEEEECCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEE
PAGAQGPGFLMLQNFRVIMKYNPAEAYALAIGHFADRLRGAPPFVQAWPREERTLSRAER
CCCCCCCCEEEEECCEEEEEECCHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHH
LELQQLLANRGFYRGTPDGQFGGQTREALRNFQASIGAPADGFATTDVLERLRGR
HHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]