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The map label for this gene is cysW [H]

Identifier: 146338174

GI number: 146338174

Start: 1139470

End: 1140372

Strand: Direct

Name: cysW [H]

Synonym: BRADO1072

Alternate gene names: 146338174

Gene position: 1139470-1140372 (Clockwise)

Preceding gene: 146338173

Following gene: 146338175

Centisome position: 15.28

GC content: 64.45

Gene sequence:

>903_bases
ATGAGCATCGAGATGACCCTGCAACGGACGCCGGATGCCTCGGCGCGCAGCCTGGCCGAAAGCGCCGCCCGACGCGATCC
GCGCGCCGAGCCGACCGTCGTTCGGCTGGCGATCATCACGCTCGCGATCGCCTTCCTGTCCATCTTTGTCGTGCTGCCGC
TGGTCGTCGTCTTCGTCGAGGCGTTCGGCAAGGGTGTCGTGGCCTATTTTTCAGCTCTGGCCGACCCCGAGGCCTGGTCC
GCCATTCGTCTCACGCTGGGTGTCGCGGCGGTGTCCGTGGGCACCAACCTGCTGTTCGGCTTGGCCGCCGCCTGGGCGAT
CGCGAAGTTCGACTTCCCCGGCAAGACCTTCCTGATCACGCTGATCGACCTGCCGTTCTCGGTCAGCCCGGTGATCTCGG
GTCTGGTGTTCGTGCTGCTGTTCGGTGCGCAGGGCTTCTTCGGACCCTGGCTGCAGGCGCACGGTCTACAGATCCTGTTC
GCCTTCCCGGGCATCGCGCTCGCCACCATCTTCGTTACGTTTCCCTTCGTCGCGCGCGCGCTGATTCCGCTGATGCAGGA
GCAGGGCACGCAGGAAGAGGAGGCGGCGATCTCGCTTGGCGCCTCGGGGCTGCAGACCTTCTTCCGCGTCACGCTGCCCA
ACATCAAATGGGGCGTGCTGTACGGCGTCCTGCTGTGCAATGCGCGGGCGATGGGTGAGTTCGGCGCGGTGTCGGTGGTG
TCGGGTCATGTCCGCGGCGAGACCAACACGATGCCGCTGCTGGTCGAGATTCTCTACAACGAGTACCAGATGGTGGCCTC
GTTCGCGATTGCCTCGCTGCTCGCGATGCTGGCGCTGATCACGCTGATTGCCAAGACCATTCTCGAACGTAACATCGACG
AGGACATGCCTCATGACCATTGA

Upstream 100 bases:

>100_bases
CATTGCAGTGGTGATGCTTGTGGTGGCATTCGTGATCATCTTCGCGGTGAATCGCCTGCAGCGCTGGGCTCAGACCCAGG
CGTCCACCTGAGGCCGCGAC

Downstream 100 bases:

>100_bases
AGCGAGAGGCGTCGTCAAGACCTTCGGGACCTTCAAGGCGCTCGACGGCGTCGACCTCAAGGTCGCCAATGGCGAGCTGC
TGGCGCTGCTCGGGCCGTCC

Product: sulfate/thiosulfate permease W

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 299

Protein sequence:

>300_residues
MSIEMTLQRTPDASARSLAESAARRDPRAEPTVVRLAIITLAIAFLSIFVVLPLVVVFVEAFGKGVVAYFSALADPEAWS
AIRLTLGVAAVSVGTNLLFGLAAAWAIAKFDFPGKTFLITLIDLPFSVSPVISGLVFVLLFGAQGFFGPWLQAHGLQILF
AFPGIALATIFVTFPFVARALIPLMQEQGTQEEEAAISLGASGLQTFFRVTLPNIKWGVLYGVLLCNARAMGEFGAVSVV
SGHVRGETNTMPLLVEILYNEYQMVASFAIASLLAMLALITLIAKTILERNIDEDMPHDH

Sequences:

>Translated_300_residues
MSIEMTLQRTPDASARSLAESAARRDPRAEPTVVRLAIITLAIAFLSIFVVLPLVVVFVEAFGKGVVAYFSALADPEAWS
AIRLTLGVAAVSVGTNLLFGLAAAWAIAKFDFPGKTFLITLIDLPFSVSPVISGLVFVLLFGAQGFFGPWLQAHGLQILF
AFPGIALATIFVTFPFVARALIPLMQEQGTQEEEAAISLGASGLQTFFRVTLPNIKWGVLYGVLLCNARAMGEFGAVSVV
SGHVRGETNTMPLLVEILYNEYQMVASFAIASLLAMLALITLIAKTILERNIDEDMPHDH
>Mature_299_residues
SIEMTLQRTPDASARSLAESAARRDPRAEPTVVRLAIITLAIAFLSIFVVLPLVVVFVEAFGKGVVAYFSALADPEAWSA
IRLTLGVAAVSVGTNLLFGLAAAWAIAKFDFPGKTFLITLIDLPFSVSPVISGLVFVLLFGAQGFFGPWLQAHGLQILFA
FPGIALATIFVTFPFVARALIPLMQEQGTQEEEAAISLGASGLQTFFRVTLPNIKWGVLYGVLLCNARAMGEFGAVSVVS
GHVRGETNTMPLLVEILYNEYQMVASFAIASLLAMLALITLIAKTILERNIDEDMPHDH

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4208

COG function: function code P; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87082099, Length=264, Percent_Identity=51.5151515151515, Blast_Score=281, Evalue=5e-77,
Organism=Escherichia coli, GI1788764, Length=206, Percent_Identity=36.4077669902913, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI1786980, Length=227, Percent_Identity=33.4801762114537, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1787369, Length=178, Percent_Identity=25.8426966292135, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011866
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32272; Mature: 32141

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIEMTLQRTPDASARSLAESAARRDPRAEPTVVRLAIITLAIAFLSIFVVLPLVVVFVE
CCEEEEECCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFGKGVVAYFSALADPEAWSAIRLTLGVAAVSVGTNLLFGLAAAWAIAKFDFPGKTFLIT
HHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
LIDLPFSVSPVISGLVFVLLFGAQGFFGPWLQAHGLQILFAFPGIALATIFVTFPFVARA
EECCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHH
LIPLMQEQGTQEEEAAISLGASGLQTFFRVTLPNIKWGVLYGVLLCNARAMGEFGAVSVV
HHHHHHHCCCCHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHCCCCCCHHHH
SGHVRGETNTMPLLVEILYNEYQMVASFAIASLLAMLALITLIAKTILERNIDEDMPHDH
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SIEMTLQRTPDASARSLAESAARRDPRAEPTVVRLAIITLAIAFLSIFVVLPLVVVFVE
CEEEEECCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFGKGVVAYFSALADPEAWSAIRLTLGVAAVSVGTNLLFGLAAAWAIAKFDFPGKTFLIT
HHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
LIDLPFSVSPVISGLVFVLLFGAQGFFGPWLQAHGLQILFAFPGIALATIFVTFPFVARA
EECCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHH
LIPLMQEQGTQEEEAAISLGASGLQTFFRVTLPNIKWGVLYGVLLCNARAMGEFGAVSVV
HHHHHHHCCCCHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHCCCCCCHHHH
SGHVRGETNTMPLLVEILYNEYQMVASFAIASLLAMLALITLIAKTILERNIDEDMPHDH
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]