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The map label for this gene is cysT [H]

Identifier: 146338173

GI number: 146338173

Start: 1138627

End: 1139460

Strand: Direct

Name: cysT [H]

Synonym: BRADO1071

Alternate gene names: 146338173

Gene position: 1138627-1139460 (Clockwise)

Preceding gene: 146338172

Following gene: 146338174

Centisome position: 15.27

GC content: 67.15

Gene sequence:

>834_bases
TTGAGCGTCCTTGCACCGCGACGCAGCACCTTGCCGGGCTTCGGTCTCACCATGGGACTGACGCTGACCTGGCTGTCCCT
GATCGTCCTCATTCCCCTGGCCGGGCTGTTCGTCCGCTCGACGGATCTCAGCCTGGCGCAGTTCTGGGACATCGTCACCA
GCCGGCGCACCCTGAGCGCGCTCAAGATCTCGTTCGGGCTGTCGTTCGCGGCGGCGCTCGTCAATCTGGTGATGGGCACC
ATCGTCGTCTGGGCGCTGGTGCGCTACCGCTTCCCCGGCCGGCGGCTGTTCGATGCCATCGTCGACGTGCCGTTCGCGCT
GCCGACCGCGGTTGCCGGCGTGGCGCTGACCGCGCTGTTCGCGCAGAAGGGCTGGCTCGGCGCGCCGCTGGCGGCGGCCG
GCATCAGGGTGGCGTTCACGCCGCTCGGCATCTTCATCGCGATGATCTTCATCGGCATCCCCTTCGTGGTCCGCACCGTG
CAGCCGGTGCTGATCGATCTCGATCCCGAGATCGAGGAAGCGGCGGCGAGCCTCGGCGCCAGCCGTTGGCACATCGTCAC
CAAGGTCATCCTGCCGAGCCTGACACCGGCGCTGCTGACCGGCTTCGCGCTCGCCTTTGCCCGGGCCGTCGGCGAGTACG
GCTCGGTGATCTTCATCGCCGGCAATCTCCCGAATGTCTCCGAGATCGCCCCCCTCCTGATCGTGATCCGCCTGTCCGAG
TTCCGCTATGCCGACGCCACGGCCATTGCAGTGGTGATGCTTGTGGTGGCATTCGTGATCATCTTCGCGGTGAATCGCCT
GCAGCGCTGGGCTCAGACCCAGGCGTCCACCTGA

Upstream 100 bases:

>100_bases
CGCAGAAGGAACACTTCGGCGAAGGTGGCGTGTTCGATCAGATCTACAAGAACTGATGACGAGCAGGAAGTAGGGACATC
GGAATGAGCCAGGTGGCGTC

Downstream 100 bases:

>100_bases
GGCCGCGACATGAGCATCGAGATGACCCTGCAACGGACGCCGGATGCCTCGGCGCGCAGCCTGGCCGAAAGCGCCGCCCG
ACGCGATCCGCGCGCCGAGC

Product: sulfate/thiosulfate ABC transporter membrane protein

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MSVLAPRRSTLPGFGLTMGLTLTWLSLIVLIPLAGLFVRSTDLSLAQFWDIVTSRRTLSALKISFGLSFAAALVNLVMGT
IVVWALVRYRFPGRRLFDAIVDVPFALPTAVAGVALTALFAQKGWLGAPLAAAGIRVAFTPLGIFIAMIFIGIPFVVRTV
QPVLIDLDPEIEEAAASLGASRWHIVTKVILPSLTPALLTGFALAFARAVGEYGSVIFIAGNLPNVSEIAPLLIVIRLSE
FRYADATAIAVVMLVVAFVIIFAVNRLQRWAQTQAST

Sequences:

>Translated_277_residues
MSVLAPRRSTLPGFGLTMGLTLTWLSLIVLIPLAGLFVRSTDLSLAQFWDIVTSRRTLSALKISFGLSFAAALVNLVMGT
IVVWALVRYRFPGRRLFDAIVDVPFALPTAVAGVALTALFAQKGWLGAPLAAAGIRVAFTPLGIFIAMIFIGIPFVVRTV
QPVLIDLDPEIEEAAASLGASRWHIVTKVILPSLTPALLTGFALAFARAVGEYGSVIFIAGNLPNVSEIAPLLIVIRLSE
FRYADATAIAVVMLVVAFVIIFAVNRLQRWAQTQAST
>Mature_276_residues
SVLAPRRSTLPGFGLTMGLTLTWLSLIVLIPLAGLFVRSTDLSLAQFWDIVTSRRTLSALKISFGLSFAAALVNLVMGTI
VVWALVRYRFPGRRLFDAIVDVPFALPTAVAGVALTALFAQKGWLGAPLAAAGIRVAFTPLGIFIAMIFIGIPFVVRTVQ
PVLIDLDPEIEEAAASLGASRWHIVTKVILPSLTPALLTGFALAFARAVGEYGSVIFIAGNLPNVSEIAPLLIVIRLSEF
RYADATAIAVVMLVVAFVIIFAVNRLQRWAQTQAST

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=261, Percent_Identity=49.8084291187739, Blast_Score=235, Evalue=2e-63,
Organism=Escherichia coli, GI87082099, Length=251, Percent_Identity=30.2788844621514, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1786980, Length=174, Percent_Identity=34.4827586206897, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1786254, Length=212, Percent_Identity=26.8867924528302, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1787368, Length=209, Percent_Identity=30.622009569378, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787369, Length=155, Percent_Identity=29.0322580645161, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29703; Mature: 29572

Theoretical pI: Translated: 11.27; Mature: 11.27

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SVLAPRRSTLPGFGLTMGLTLTWLSLIVLIPLAGLFVRSTDLSLAQFWDIVTSRRTLSA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LKISFGLSFAAALVNLVMGTIVVWALVRYRFPGRRLFDAIVDVPFALPTAVAGVALTALF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHH
AQKGWLGAPLAAAGIRVAFTPLGIFIAMIFIGIPFVVRTVQPVLIDLDPEIEEAAASLGA
HHCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHCC
SRWHIVTKVILPSLTPALLTGFALAFARAVGEYGSVIFIAGNLPNVSEIAPLLIVIRLSE
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHH
FRYADATAIAVVMLVVAFVIIFAVNRLQRWAQTQAST
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503; 2188959 [H]