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The map label for this gene is spoT [H]

Identifier: 146338142

GI number: 146338142

Start: 1107095

End: 1107622

Strand: Direct

Name: spoT [H]

Synonym: BRADO1039

Alternate gene names: 146338142

Gene position: 1107095-1107622 (Clockwise)

Preceding gene: 146338141

Following gene: 146338143

Centisome position: 14.85

GC content: 67.23

Gene sequence:

>528_bases
TTGCGCCTCGTCTCCGAAGCGGCCGAGCTGGCGGCACGCCGCCACGCCGGCCAGCAGCGCAAGGGCCGCGACGAGGAGCC
CTATGTCAATCATCTCGCCGAGGTGGCAAACATCCTTGCCGCCACGACTGACGGTGCCGACGCCGAGCTGGTCGCGGCCG
GCTGGCTTCACGACACGATCGAGGATACCGACACCACGCGGGATGAGCTCGCGCAGCGCTTCGGGGCGCGCGTGGCCGGC
CTCGTGGAGGAGGTGACCGACGACATGACGTTGCCGAAGAGCGAACGGCGCGCCGTCCAGGTCATTGCCGCGCCGGCCAA
ATCCGACGGCGCCAAGCAGATCAAGATCGCCGACAAGATCAGCAACATCCGTGCGCGTGTCTTTTTCGAGCCGGATCTCG
AGCAGCAGCTCGAACTGATGGAGTATGTCGGCTGGGCCGAGCAGGTCGTCGCCGGCTGCCGCGGCGTCAACGCGCGGCTG
GATGCGCTGTTCGACGAGACCGTTTCGAATGCGAGAGGGACGCTGTGA

Upstream 100 bases:

>100_bases
CAGACCCGCGACGTGCAGCTGGTGGCGCTCGCCGGCAAGCGCATGGTCTATGGCTTCCGCGGCGACGTCATGGGCAAGCT
GTAGACCGATGTTGACCGGG

Downstream 100 bases:

>100_bases
GCGGCTTGGCGCGTATGTCCTCGGCGGCCGTCTACAGCCTGTGCGCGGCATTGGCAATTGCAGGGCTTACTGCTGCCTCG
GCAGCGGACGACCGGCGCTG

Product: putative metal-dependent phosphohydrolase

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 175; Mature: 175

Protein sequence:

>175_residues
MRLVSEAAELAARRHAGQQRKGRDEEPYVNHLAEVANILAATTDGADAELVAAGWLHDTIEDTDTTRDELAQRFGARVAG
LVEEVTDDMTLPKSERRAVQVIAAPAKSDGAKQIKIADKISNIRARVFFEPDLEQQLELMEYVGWAEQVVAGCRGVNARL
DALFDETVSNARGTL

Sequences:

>Translated_175_residues
MRLVSEAAELAARRHAGQQRKGRDEEPYVNHLAEVANILAATTDGADAELVAAGWLHDTIEDTDTTRDELAQRFGARVAG
LVEEVTDDMTLPKSERRAVQVIAAPAKSDGAKQIKIADKISNIRARVFFEPDLEQQLELMEYVGWAEQVVAGCRGVNARL
DALFDETVSNARGTL
>Mature_175_residues
MRLVSEAAELAARRHAGQQRKGRDEEPYVNHLAEVANILAATTDGADAELVAAGWLHDTIEDTDTTRDELAQRFGARVAG
LVEEVTDDMTLPKSERRAVQVIAAPAKSDGAKQIKIADKISNIRARVFFEPDLEQQLELMEYVGWAEQVVAGCRGVNARL
DALFDETVSNARGTL

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Homo sapiens, GI38348360, Length=119, Percent_Identity=53.781512605042, Blast_Score=120, Evalue=4e-28,
Organism=Escherichia coli, GI1790082, Length=132, Percent_Identity=37.1212121212121, Blast_Score=60, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI25143118, Length=174, Percent_Identity=36.2068965517241, Blast_Score=105, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24650996, Length=170, Percent_Identity=40.5882352941176, Blast_Score=117, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 19157; Mature: 19157

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLVSEAAELAARRHAGQQRKGRDEEPYVNHLAEVANILAATTDGADAELVAAGWLHDTI
CCHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
EDTDTTRDELAQRFGARVAGLVEEVTDDMTLPKSERRAVQVIAAPAKSDGAKQIKIADKI
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHH
SNIRARVFFEPDLEQQLELMEYVGWAEQVVAGCRGVNARLDALFDETVSNARGTL
HHHHEEEEECCCHHHHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRLVSEAAELAARRHAGQQRKGRDEEPYVNHLAEVANILAATTDGADAELVAAGWLHDTI
CCHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
EDTDTTRDELAQRFGARVAGLVEEVTDDMTLPKSERRAVQVIAAPAKSDGAKQIKIADKI
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHH
SNIRARVFFEPDLEQQLELMEYVGWAEQVVAGCRGVNARLDALFDETVSNARGTL
HHHHEEEEECCCHHHHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]