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The map label for this gene is livM [H]

Identifier: 146338136

GI number: 146338136

Start: 1102766

End: 1103929

Strand: Direct

Name: livM [H]

Synonym: BRADO1033

Alternate gene names: 146338136

Gene position: 1102766-1103929 (Clockwise)

Preceding gene: 146338135

Following gene: 146338137

Centisome position: 14.79

GC content: 65.12

Gene sequence:

>1164_bases
ATGATGCCGCATCTGCTGACCCGCTCGCTCGACCGCGGCGCGTCGATCTTTCTCCTGATCGTCGCCGCCTGCGGCATCCT
GATCCCGCTGTCCAACCTGCTGCTGCCGGCGGGTTCGGCGCTGCAGGTGCCGACCTATCTGGTCGCGCTGTGGGGCAAAT
ATGTCTGCTACGCCATCCTGGCGCTCTCGATCGATCTGATCTGGGGCTATTGCGGCATTCTCTCGCTCGGTCATGGCGCC
TTTTTCGCGCTCGGCGGCTACGCCATGGGCATGTATCTGATGCGCCAGATCGGCACCCGCGGCGTCTACGGCAACCCGAT
CCTGCCGGACTTCATGGTGTTCCTGAACTACTCCAAGCTGCCCTGGTACTGGCACGGCTTCGACATGTTCTGGTTCGCCG
CGCTGATGGTGCTGTTGGTGCCCGGCCTGCTCGCCTTCTGCTTCGGCTGGCTCGCCTTCCGCTCCCGCGTCACCGGCGTC
TATCTCTCGATCATCACCCAGGCGATGACCTACGCGCTGCTGCTCGCGTTCTTCCGCAACGATTTCGGCTTCGGCGGCAA
CAACGGCCTGACCGACTTCAAGGACATCCTCGGCTTCAACGTCCAGGCGGACGGCACCCGCGCGGCCCTGTTCGCGCTCA
GCTGCCTCGCGTTGATCCTCGCCTTCCTGATCTGCCGTGCAGTGGTGTCGTCGAAGCTCGGCAAGGTGCTGATCGCGATC
CGCGACGCCGAATCGCGCACGCGCTTCCTCGGCTACCGGGTGGAGTCCTACAAGCTGTTCGTCTTCACGCTGTCGGCCTG
CATGGCCGGCGTCGCCGGCGCGCTCTATGTGCCGCAGGTCGGCATCATCAACCCATCCGAGTTTGCGCCGGGCAATTCGA
TCGAGGCGGTGATCTGGGTTGCGGTCGGAGGCCGCGGCACGCTGGTCGGGGCGGCGCTCGGTGCCGTCGTGGTCAACTAC
GCCAAGACGTTCTTCACCTCGGGCGTGCTGGCGCCTTACTGGCTGTTCATGCTCGGCGCGCTGTTCGTCATGGTCACCTT
GCTGCTGCCGAAGGGCATTCTCGGCACGTTCAATGCGTGGTGGGACCAGCTCGGCACCAAGACGCCGAACGCCGACAGCG
CCGCCCGCGAGGACGGCGTCACCGAACCCAAGATGGCGGAGTGA

Upstream 100 bases:

>100_bases
GCGCGGTGCTCGGCAAGATCGCGATCCTGGTGCTGATCATCCTTTTCATTCAGAAGCGCCCGCGCGGGCTGTTCGCGCTC
AAGGGCCGGGCGGTGGAAGC

Downstream 100 bases:

>100_bases
GCCCGTGAACGTGATGGACACCCGCGCGACCTCGTCGCTGCTCTATCTCGATGGCGTGCACGTCTCCTTCGACGGTTTCC
ATGCCATCAACAATCTCTCG

Product: branched chain amino acid ABC transporter permease

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: LIV-I protein M [H]

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MMPHLLTRSLDRGASIFLLIVAACGILIPLSNLLLPAGSALQVPTYLVALWGKYVCYAILALSIDLIWGYCGILSLGHGA
FFALGGYAMGMYLMRQIGTRGVYGNPILPDFMVFLNYSKLPWYWHGFDMFWFAALMVLLVPGLLAFCFGWLAFRSRVTGV
YLSIITQAMTYALLLAFFRNDFGFGGNNGLTDFKDILGFNVQADGTRAALFALSCLALILAFLICRAVVSSKLGKVLIAI
RDAESRTRFLGYRVESYKLFVFTLSACMAGVAGALYVPQVGIINPSEFAPGNSIEAVIWVAVGGRGTLVGAALGAVVVNY
AKTFFTSGVLAPYWLFMLGALFVMVTLLLPKGILGTFNAWWDQLGTKTPNADSAAREDGVTEPKMAE

Sequences:

>Translated_387_residues
MMPHLLTRSLDRGASIFLLIVAACGILIPLSNLLLPAGSALQVPTYLVALWGKYVCYAILALSIDLIWGYCGILSLGHGA
FFALGGYAMGMYLMRQIGTRGVYGNPILPDFMVFLNYSKLPWYWHGFDMFWFAALMVLLVPGLLAFCFGWLAFRSRVTGV
YLSIITQAMTYALLLAFFRNDFGFGGNNGLTDFKDILGFNVQADGTRAALFALSCLALILAFLICRAVVSSKLGKVLIAI
RDAESRTRFLGYRVESYKLFVFTLSACMAGVAGALYVPQVGIINPSEFAPGNSIEAVIWVAVGGRGTLVGAALGAVVVNY
AKTFFTSGVLAPYWLFMLGALFVMVTLLLPKGILGTFNAWWDQLGTKTPNADSAAREDGVTEPKMAE
>Mature_387_residues
MMPHLLTRSLDRGASIFLLIVAACGILIPLSNLLLPAGSALQVPTYLVALWGKYVCYAILALSIDLIWGYCGILSLGHGA
FFALGGYAMGMYLMRQIGTRGVYGNPILPDFMVFLNYSKLPWYWHGFDMFWFAALMVLLVPGLLAFCFGWLAFRSRVTGV
YLSIITQAMTYALLLAFFRNDFGFGGNNGLTDFKDILGFNVQADGTRAALFALSCLALILAFLICRAVVSSKLGKVLIAI
RDAESRTRFLGYRVESYKLFVFTLSACMAGVAGALYVPQVGIINPSEFAPGNSIEAVIWVAVGGRGTLVGAALGAVVVNY
AKTFFTSGVLAPYWLFMLGALFVMVTLLLPKGILGTFNAWWDQLGTKTPNADSAAREDGVTEPKMAE

Specific function: Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=322, Percent_Identity=24.223602484472, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 41994; Mature: 41994

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMPHLLTRSLDRGASIFLLIVAACGILIPLSNLLLPAGSALQVPTYLVALWGKYVCYAIL
CCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
ALSIDLIWGYCGILSLGHGAFFALGGYAMGMYLMRQIGTRGVYGNPILPDFMVFLNYSKL
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCC
PWYWHGFDMFWFAALMVLLVPGLLAFCFGWLAFRSRVTGVYLSIITQAMTYALLLAFFRN
CCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DFGFGGNNGLTDFKDILGFNVQADGTRAALFALSCLALILAFLICRAVVSSKLGKVLIAI
CCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEE
RDAESRTRFLGYRVESYKLFVFTLSACMAGVAGALYVPQVGIINPSEFAPGNSIEAVIWV
ECCHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEE
AVGGRGTLVGAALGAVVVNYAKTFFTSGVLAPYWLFMLGALFVMVTLLLPKGILGTFNAW
EECCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
WDQLGTKTPNADSAAREDGVTEPKMAE
HHHHCCCCCCCCCHHHHCCCCCCCCCC
>Mature Secondary Structure
MMPHLLTRSLDRGASIFLLIVAACGILIPLSNLLLPAGSALQVPTYLVALWGKYVCYAIL
CCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
ALSIDLIWGYCGILSLGHGAFFALGGYAMGMYLMRQIGTRGVYGNPILPDFMVFLNYSKL
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCC
PWYWHGFDMFWFAALMVLLVPGLLAFCFGWLAFRSRVTGVYLSIITQAMTYALLLAFFRN
CCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DFGFGGNNGLTDFKDILGFNVQADGTRAALFALSCLALILAFLICRAVVSSKLGKVLIAI
CCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEE
RDAESRTRFLGYRVESYKLFVFTLSACMAGVAGALYVPQVGIINPSEFAPGNSIEAVIWV
ECCHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEE
AVGGRGTLVGAALGAVVVNYAKTFFTSGVLAPYWLFMLGALFVMVTLLLPKGILGTFNAW
EECCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
WDQLGTKTPNADSAAREDGVTEPKMAE
HHHHCCCCCCCCCHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2195019; 8041620; 9278503 [H]