Click here to switch to the map view.

The map label for this gene is hcl [H]

Identifier: 146338068

GI number: 146338068

Start: 1021290

End: 1023191

Strand: Direct

Name: hcl [H]

Synonym: BRADO0963

Alternate gene names: 146338068

Gene position: 1021290-1023191 (Clockwise)

Preceding gene: 146338067

Following gene: 146338069

Centisome position: 13.7

GC content: 65.77

Gene sequence:

>1902_bases
ATGACCGCGCAAACAACAAAAATCACACAGGGAGGAACGGACAGCACGGCGCGGAGCGCCAGTGTTCATCCGTTGCGCGC
CATCTCCTTCAATGATCCCGTCGTCGACGTCGAGCGCCGCGACGACGGCACCATCTATCTCCGGCCGACGCGTCCGCTCG
GCGACTTTCCGCAGCGGATCACCGATCGGCTGCATCACTTCGCCAAGGAAACGCCGGAGCGCGTGTTCATGGCCGAGCGC
GTCGGCCCCGAAGGCTGGCGCGAGCTGAGCTATGGCACCTTGCTGGCGGCAAGCCGGCATATCGCCTCCGGCCTGCTCGC
CCGCGGCCTGTCGCCGGACCGCCCGGTGATGATCCTGTCCGGCAATTCGATCGACCACGCGCTAGTCGCGTTCGGCTCGC
TCTATGCCGGCGTGCCGTTCTGCCCGGTGTCGCCGGCCTATTCGCTGGTGTCGCGCGATTACGGCAAGCTCGCCTACCTG
ATGAAGCTGCTGACGCCGGGTCTCGTCTTCGTCGACGACGCCGACAAGTTTGCCGACGCGCTGATCGCCAACGCGCCGGA
AGGCACCGAGATCGTCGCATCCTTCGGCGAAGTCCCGGGCCGCAAGATCACGATGCTGTCGGAGCTGATCGCCTCGCCGC
TGTTCGCCGGTCTCGATGCTGTCCATGACAAGATCGGCCCGGACACGATCGCGAAATTCCTGCTGACCTCGGGCTCGACC
GGCAATCCCAAGGCCGTCATCAACACCCAGCGCATGATCTGCGCCAACCAGGTGATGATCCGCGAGACGCTCGCCTTCCT
GAAGGACGAGCCGCCCGTCATCGTCGACTGGCTACCGTGGAACCACACCTTCGGTGGCAACCACAACATCGGTCTCACGC
TGTTCAACGGCGGCTCGATGTATCTCGACCAGGGCAAGCCGGTGCCCGGCGGCATCGAGGAGACCGTGCGCAACCTGCGG
GAGATCTCGCCGACGGTCTATTTCAACGTCCCCAAGGGCTACGAGTCGCTGCTGCCCTATTTCCGCGAGGACCCCAATCT
GCGGAAGACGTTCTTCCGCCGGCTGCATGCGATGTTCTTCTCCGGCGCCGCGCTCGCGCCGCACGTCTGGAACGAGCTCG
ACGAGCTCTCCGTGGCCGAAACGGGGTATCGCGTGCCGATGCTGACGGGACTCGGCTCGACCGAGACCGCGCCGTTCTTC
ATGTCGGTCAATCCGCGCACCAGCCGCTCCGGCCATGTCGGCCTTCCCGTGCCAGGCAACGAGGCCAAGCTCGTGCCGAA
CAACGGCAAGATGGAAGTGCGCGCCAAGGGCCCGAACGTGACGCCCGGATATTGGCGTCTTCCCGAAGTCTCGGCCGCCG
CCTTCGACTCCGAGGGCTACTACCAGATGGGCGACGCGCTGAAGCCCGCCGATCCCAACGACTTCAATGCCGGGTTCGAT
TTCGACGGCCGCGTCGCCGAGGACTTCAAGCTGGCGTCGGGCACCTGGGTGTCGGTCGGCCCCTTGCGCGCGCGCTTCGT
CGCGGCCTGCGCACCGCTGGCGCGCGACGTCATCATCGCCGGCATCAACCGCGACGAGATCGCGGCCATCGTCGTGCTCG
ACCTCGACGGCTGCCGCCTGATCAACGCGACATTGCCGTTCGATGATCTCGCCGAAACCGCGTCGGATCCGCTGATCGTG
GCCGCGTTCCGCGAGCGCTTCACGAAATTCCTGAAGACCGCGACCGGCTCCTCGACGCGGATCACGCGCGCGGTGCTGCT
CGGCCTGCCCTTGTCGATCGACAAGGGCGAGGTCACCGACAAGGGCTCGATCAACCAGCGCGCCGTGCTGGAGAACCGCA
AGGACCTGATCGAGCGCATCTACGCGTCGACACCGGACGACGACATCATCATCGCCGGCTGA

Upstream 100 bases:

>100_bases
GAATCGCTGATGGCCACCGTCGCGCAGAGCGACAAGGAGGCCAAGGGCCGCATCCGCGCCTTTCTCGATCACAAGACAGC
CAAAGTGAAGCCGACGTGAC

Downstream 100 bases:

>100_bases
TCAACGAAATAAGGGAGAGACGACATGTTGTTGAAGGATCAGGCCGCCATCGTCACCGGCGGCGCCTCGGGCCTCGGCGC
CGCCACCGCCCGCAAGCTCG

Product: feruloyl-CoA synthase

Products: NA

Alternate protein names: 3-hydroxybenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 633; Mature: 632

Protein sequence:

>633_residues
MTAQTTKITQGGTDSTARSASVHPLRAISFNDPVVDVERRDDGTIYLRPTRPLGDFPQRITDRLHHFAKETPERVFMAER
VGPEGWRELSYGTLLAASRHIASGLLARGLSPDRPVMILSGNSIDHALVAFGSLYAGVPFCPVSPAYSLVSRDYGKLAYL
MKLLTPGLVFVDDADKFADALIANAPEGTEIVASFGEVPGRKITMLSELIASPLFAGLDAVHDKIGPDTIAKFLLTSGST
GNPKAVINTQRMICANQVMIRETLAFLKDEPPVIVDWLPWNHTFGGNHNIGLTLFNGGSMYLDQGKPVPGGIEETVRNLR
EISPTVYFNVPKGYESLLPYFREDPNLRKTFFRRLHAMFFSGAALAPHVWNELDELSVAETGYRVPMLTGLGSTETAPFF
MSVNPRTSRSGHVGLPVPGNEAKLVPNNGKMEVRAKGPNVTPGYWRLPEVSAAAFDSEGYYQMGDALKPADPNDFNAGFD
FDGRVAEDFKLASGTWVSVGPLRARFVAACAPLARDVIIAGINRDEIAAIVVLDLDGCRLINATLPFDDLAETASDPLIV
AAFRERFTKFLKTATGSSTRITRAVLLGLPLSIDKGEVTDKGSINQRAVLENRKDLIERIYASTPDDDIIIAG

Sequences:

>Translated_633_residues
MTAQTTKITQGGTDSTARSASVHPLRAISFNDPVVDVERRDDGTIYLRPTRPLGDFPQRITDRLHHFAKETPERVFMAER
VGPEGWRELSYGTLLAASRHIASGLLARGLSPDRPVMILSGNSIDHALVAFGSLYAGVPFCPVSPAYSLVSRDYGKLAYL
MKLLTPGLVFVDDADKFADALIANAPEGTEIVASFGEVPGRKITMLSELIASPLFAGLDAVHDKIGPDTIAKFLLTSGST
GNPKAVINTQRMICANQVMIRETLAFLKDEPPVIVDWLPWNHTFGGNHNIGLTLFNGGSMYLDQGKPVPGGIEETVRNLR
EISPTVYFNVPKGYESLLPYFREDPNLRKTFFRRLHAMFFSGAALAPHVWNELDELSVAETGYRVPMLTGLGSTETAPFF
MSVNPRTSRSGHVGLPVPGNEAKLVPNNGKMEVRAKGPNVTPGYWRLPEVSAAAFDSEGYYQMGDALKPADPNDFNAGFD
FDGRVAEDFKLASGTWVSVGPLRARFVAACAPLARDVIIAGINRDEIAAIVVLDLDGCRLINATLPFDDLAETASDPLIV
AAFRERFTKFLKTATGSSTRITRAVLLGLPLSIDKGEVTDKGSINQRAVLENRKDLIERIYASTPDDDIIIAG
>Mature_632_residues
TAQTTKITQGGTDSTARSASVHPLRAISFNDPVVDVERRDDGTIYLRPTRPLGDFPQRITDRLHHFAKETPERVFMAERV
GPEGWRELSYGTLLAASRHIASGLLARGLSPDRPVMILSGNSIDHALVAFGSLYAGVPFCPVSPAYSLVSRDYGKLAYLM
KLLTPGLVFVDDADKFADALIANAPEGTEIVASFGEVPGRKITMLSELIASPLFAGLDAVHDKIGPDTIAKFLLTSGSTG
NPKAVINTQRMICANQVMIRETLAFLKDEPPVIVDWLPWNHTFGGNHNIGLTLFNGGSMYLDQGKPVPGGIEETVRNLRE
ISPTVYFNVPKGYESLLPYFREDPNLRKTFFRRLHAMFFSGAALAPHVWNELDELSVAETGYRVPMLTGLGSTETAPFFM
SVNPRTSRSGHVGLPVPGNEAKLVPNNGKMEVRAKGPNVTPGYWRLPEVSAAAFDSEGYYQMGDALKPADPNDFNAGFDF
DGRVAEDFKLASGTWVSVGPLRARFVAACAPLARDVIIAGINRDEIAAIVVLDLDGCRLINATLPFDDLAETASDPLIVA
AFRERFTKFLKTATGSSTRITRAVLLGLPLSIDKGEVTDKGSINQRAVLENRKDLIERIYASTPDDDIIIAG

Specific function: Catalyzes the ligation of 3-hydroxybenzoate or 4- hydroxybenzoate and CoA at the expense of ATP. The enzyme shows low activity towards benzoate, 4-aminobenzoate, 3-aminobenzoate, 3-fluorobenzoate, 4-fluorobenzoate, 3-chlorobenzoate, and 4- chlorobenzoate.

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. Benzoate-CoA ligase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788107, Length=418, Percent_Identity=25.3588516746411, Blast_Score=74, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17564090, Length=450, Percent_Identity=24.6666666666667, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873
- InterPro:   IPR011957 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.27 [H]

Molecular weight: Translated: 68862; Mature: 68731

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAQTTKITQGGTDSTARSASVHPLRAISFNDPVVDVERRDDGTIYLRPTRPLGDFPQRI
CCCCCCEEECCCCCCCCCCCCCCCEEEEECCCCEEEEEECCCCEEEEEECCCCCHHHHHH
TDRLHHFAKETPERVFMAERVGPEGWRELSYGTLLAASRHIASGLLARGLSPDRPVMILS
HHHHHHHHHCCHHHHHHHHHCCCHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
GNSIDHALVAFGSLYAGVPFCPVSPAYSLVSRDYGKLAYLMKLLTPGLVFVDDADKFADA
CCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHCCHHHHHHHHHHHCCCEEEEECHHHHHHH
LIANAPEGTEIVASFGEVPGRKITMLSELIASPLFAGLDAVHDKIGPDTIAKFLLTSGST
HHCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCC
GNPKAVINTQRMICANQVMIRETLAFLKDEPPVIVDWLPWNHTFGGNHNIGLTLFNGGSM
CCCCEEEECHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCEEEEEECCCEE
YLDQGKPVPGGIEETVRNLREISPTVYFNVPKGYESLLPYFREDPNLRKTFFRRLHAMFF
EECCCCCCCCCHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHH
SGAALAPHVWNELDELSVAETGYRVPMLTGLGSTETAPFFMSVNPRTSRSGHVGLPVPGN
CCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEEEECCCCCCCCEECCCCCCC
EAKLVPNNGKMEVRAKGPNVTPGYWRLPEVSAAAFDSEGYYQMGDALKPADPNDFNAGFD
CCEEECCCCCEEEEECCCCCCCCCEECCCCHHHHCCCCCCEECCCCCCCCCCCCCCCCCC
FDGRVAEDFKLASGTWVSVGPLRARFVAACAPLARDVIIAGINRDEIAAIVVLDLDGCRL
CCCCCCCCEEECCCCEEEECHHHHHHHHHHHHHHHCCEEECCCCCCEEEEEEEECCCCEE
INATLPFDDLAETASDPLIVAAFRERFTKFLKTATGSSTRITRAVLLGLPLSIDKGEVTD
EEECCCHHHHHHHCCCCEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCC
KGSINQRAVLENRKDLIERIYASTPDDDIIIAG
CCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEC
>Mature Secondary Structure 
TAQTTKITQGGTDSTARSASVHPLRAISFNDPVVDVERRDDGTIYLRPTRPLGDFPQRI
CCCCCEEECCCCCCCCCCCCCCCEEEEECCCCEEEEEECCCCEEEEEECCCCCHHHHHH
TDRLHHFAKETPERVFMAERVGPEGWRELSYGTLLAASRHIASGLLARGLSPDRPVMILS
HHHHHHHHHCCHHHHHHHHHCCCHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
GNSIDHALVAFGSLYAGVPFCPVSPAYSLVSRDYGKLAYLMKLLTPGLVFVDDADKFADA
CCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHCCHHHHHHHHHHHCCCEEEEECHHHHHHH
LIANAPEGTEIVASFGEVPGRKITMLSELIASPLFAGLDAVHDKIGPDTIAKFLLTSGST
HHCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCC
GNPKAVINTQRMICANQVMIRETLAFLKDEPPVIVDWLPWNHTFGGNHNIGLTLFNGGSM
CCCCEEEECHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCEEEEEECCCEE
YLDQGKPVPGGIEETVRNLREISPTVYFNVPKGYESLLPYFREDPNLRKTFFRRLHAMFF
EECCCCCCCCCHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHH
SGAALAPHVWNELDELSVAETGYRVPMLTGLGSTETAPFFMSVNPRTSRSGHVGLPVPGN
CCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEEEECCCCCCCCEECCCCCCC
EAKLVPNNGKMEVRAKGPNVTPGYWRLPEVSAAAFDSEGYYQMGDALKPADPNDFNAGFD
CCEEECCCCCEEEEECCCCCCCCCEECCCCHHHHCCCCCCEECCCCCCCCCCCCCCCCCC
FDGRVAEDFKLASGTWVSVGPLRARFVAACAPLARDVIIAGINRDEIAAIVVLDLDGCRL
CCCCCCCCEEECCCCEEEECHHHHHHHHHHHHHHHCCEEECCCCCCEEEEEEEECCCCEE
INATLPFDDLAETASDPLIVAAFRERFTKFLKTATGSSTRITRAVLLGLPLSIDKGEVTD
EEECCCHHHHHHHCCCCEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCC
KGSINQRAVLENRKDLIERIYASTPDDDIIIAG
CCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11208796 [H]