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The map label for this gene is paaF [C]

Identifier: 146338067

GI number: 146338067

Start: 1020488

End: 1021288

Strand: Direct

Name: paaF [C]

Synonym: BRADO0962

Alternate gene names: 146338067

Gene position: 1020488-1021288 (Clockwise)

Preceding gene: 146338066

Following gene: 146338068

Centisome position: 13.69

GC content: 66.29

Gene sequence:

>801_bases
ATGGGAAACGCCGACACCACGCCAGCGGGCGATCCGTCGCTGCTGCAGATCGAACAGCAGGGCGCCGTCCTGACCATCGG
GCTCAACCGGCCCGCCAAGCGCAACGCGCTGAATGACGGCATCATCCTCGCGATCGGCGAGTGCTTCACCAGCCTGCCCG
ATGATATCCGCTGCGTCGTGATCCACGGCCTCGGCGACAATTTCTCCTCCGGCCTCGATCTCTCCGAACTGCGCGAGCGC
GACGCCACCGAAGGCCTGGTGCATTCGCAGATGTGGCACCGCGTGTTCGACCGCATCCAATATTGCCGTGTGCCGGTGAT
CGCCGCGCTGAAGGGCGCCGTCATCGGCGGCGGCCTCGAGCTCGCCTGCGCCGCGCATATCCGCGTCGCCGAGCCCTCGG
CCTATTTCGCGCTGCCCGAGGGCCAGCGCGGCATCTTCGTCGGCGGTGGCGGCTCGGTGCGGCTGCCGCGCCTGATCGGC
GTCGCCCGCATGATGGACATGATGCTGACCGGCCGCGTCTATTCCGCGACCGAAGGCTCGTCCTATGGCTTCGCGCAATA
CCTCGTCGAAGCCGGCGCGGCGACGACCAAGGCGCTGGAACTCGCGGCCAAGATCGCCAATAACGCGCCGCTGACGAATT
TCGCAGTGCTGCAGGCGCTGCCGATGATTGCGGAAGCCAATCCGCAGACCGGTCTCTTGATGGAATCGCTGATGGCCACC
GTCGCGCAGAGCGACAAGGAGGCCAAGGGCCGCATCCGCGCCTTTCTCGATCACAAGACAGCCAAAGTGAAGCCGACGTG
A

Upstream 100 bases:

>100_bases
GCTCATTTTGCATTGCACAACACGTTGTCCGGCGCCGCTTATTGACTCCTGGCAAGATCGTTATAAAACATAACTATTCT
GAACAGGACACAATCGAACG

Downstream 100 bases:

>100_bases
CATGACCGCGCAAACAACAAAAATCACACAGGGAGGAACGGACAGCACGGCGCGGAGCGCCAGTGTTCATCCGTTGCGCG
CCATCTCCTTCAATGATCCC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 266; Mature: 265

Protein sequence:

>266_residues
MGNADTTPAGDPSLLQIEQQGAVLTIGLNRPAKRNALNDGIILAIGECFTSLPDDIRCVVIHGLGDNFSSGLDLSELRER
DATEGLVHSQMWHRVFDRIQYCRVPVIAALKGAVIGGGLELACAAHIRVAEPSAYFALPEGQRGIFVGGGGSVRLPRLIG
VARMMDMMLTGRVYSATEGSSYGFAQYLVEAGAATTKALELAAKIANNAPLTNFAVLQALPMIAEANPQTGLLMESLMAT
VAQSDKEAKGRIRAFLDHKTAKVKPT

Sequences:

>Translated_266_residues
MGNADTTPAGDPSLLQIEQQGAVLTIGLNRPAKRNALNDGIILAIGECFTSLPDDIRCVVIHGLGDNFSSGLDLSELRER
DATEGLVHSQMWHRVFDRIQYCRVPVIAALKGAVIGGGLELACAAHIRVAEPSAYFALPEGQRGIFVGGGGSVRLPRLIG
VARMMDMMLTGRVYSATEGSSYGFAQYLVEAGAATTKALELAAKIANNAPLTNFAVLQALPMIAEANPQTGLLMESLMAT
VAQSDKEAKGRIRAFLDHKTAKVKPT
>Mature_265_residues
GNADTTPAGDPSLLQIEQQGAVLTIGLNRPAKRNALNDGIILAIGECFTSLPDDIRCVVIHGLGDNFSSGLDLSELRERD
ATEGLVHSQMWHRVFDRIQYCRVPVIAALKGAVIGGGLELACAAHIRVAEPSAYFALPEGQRGIFVGGGGSVRLPRLIGV
ARMMDMMLTGRVYSATEGSSYGFAQYLVEAGAATTKALELAAKIANNAPLTNFAVLQALPMIAEANPQTGLLMESLMATV
AQSDKEAKGRIRAFLDHKTAKVKPT

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=254, Percent_Identity=29.5275590551181, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI70995211, Length=271, Percent_Identity=31.7343173431734, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI20127408, Length=178, Percent_Identity=30.8988764044944, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI4502327, Length=252, Percent_Identity=30.952380952381, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI68989263, Length=193, Percent_Identity=32.1243523316062, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI31542718, Length=203, Percent_Identity=28.0788177339901, Blast_Score=72, Evalue=6e-13,
Organism=Escherichia coli, GI1787659, Length=256, Percent_Identity=34.375, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI221142681, Length=200, Percent_Identity=35.5, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1788682, Length=149, Percent_Identity=36.9127516778523, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790281, Length=187, Percent_Identity=28.8770053475936, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787660, Length=251, Percent_Identity=28.2868525896414, Blast_Score=76, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17560910, Length=211, Percent_Identity=31.7535545023697, Blast_Score=114, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17540714, Length=210, Percent_Identity=33.8095238095238, Blast_Score=102, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17534483, Length=213, Percent_Identity=31.4553990610329, Blast_Score=97, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17554946, Length=255, Percent_Identity=29.8039215686275, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI25145438, Length=244, Percent_Identity=29.0983606557377, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI25144276, Length=163, Percent_Identity=34.3558282208589, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17508951, Length=163, Percent_Identity=34.3558282208589, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17508953, Length=163, Percent_Identity=34.3558282208589, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17558304, Length=185, Percent_Identity=28.6486486486486, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17536985, Length=225, Percent_Identity=26.6666666666667, Blast_Score=77, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24653139, Length=223, Percent_Identity=32.2869955156951, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI21357171, Length=202, Percent_Identity=32.1782178217822, Blast_Score=98, Evalue=6e-21,
Organism=Drosophila melanogaster, GI20129971, Length=245, Percent_Identity=31.8367346938775, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24653477, Length=245, Percent_Identity=31.8367346938775, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI19920382, Length=226, Percent_Identity=30.9734513274336, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24650670, Length=194, Percent_Identity=30.4123711340206, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24654903, Length=182, Percent_Identity=27.4725274725275, Blast_Score=77, Evalue=9e-15,
Organism=Drosophila melanogaster, GI19921000, Length=175, Percent_Identity=34.8571428571429, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24583077, Length=175, Percent_Identity=34.8571428571429, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24583079, Length=175, Percent_Identity=34.8571428571429, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28216; Mature: 28085

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNADTTPAGDPSLLQIEQQGAVLTIGLNRPAKRNALNDGIILAIGECFTSLPDDIRCVV
CCCCCCCCCCCCCEEEEECCCCEEEEECCCCHHHCCCCCCEEEEEHHHHHCCCCCEEEEE
IHGLGDNFSSGLDLSELRERDATEGLVHSQMWHRVFDRIQYCRVPVIAALKGAVIGGGLE
EEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHEECCCEE
LACAAHIRVAEPSAYFALPEGQRGIFVGGGGSVRLPRLIGVARMMDMMLTGRVYSATEGS
EEEEEEEEEECCCEEEECCCCCCEEEEECCCCEEHHHHHHHHHHHHHHHCCCEEECCCCC
SYGFAQYLVEAGAATTKALELAAKIANNAPLTNFAVLQALPMIAEANPQTGLLMESLMAT
CCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
VAQSDKEAKGRIRAFLDHKTAKVKPT
HHHCCHHHHHHHHHHHCCCCEECCCC
>Mature Secondary Structure 
GNADTTPAGDPSLLQIEQQGAVLTIGLNRPAKRNALNDGIILAIGECFTSLPDDIRCVV
CCCCCCCCCCCCEEEEECCCCEEEEECCCCHHHCCCCCCEEEEEHHHHHCCCCCEEEEE
IHGLGDNFSSGLDLSELRERDATEGLVHSQMWHRVFDRIQYCRVPVIAALKGAVIGGGLE
EEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHEECCCEE
LACAAHIRVAEPSAYFALPEGQRGIFVGGGGSVRLPRLIGVARMMDMMLTGRVYSATEGS
EEEEEEEEEECCCEEEECCCCCCEEEEECCCCEEHHHHHHHHHHHHHHHCCCEEECCCCC
SYGFAQYLVEAGAATTKALELAAKIANNAPLTNFAVLQALPMIAEANPQTGLLMESLMAT
CCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
VAQSDKEAKGRIRAFLDHKTAKVKPT
HHHCCHHHHHHHHHHHCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA