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The map label for this gene is potB [H]

Identifier: 146337980

GI number: 146337980

Start: 924313

End: 925194

Strand: Reverse

Name: potB [H]

Synonym: BRADO0872

Alternate gene names: 146337980

Gene position: 925194-924313 (Counterclockwise)

Preceding gene: 146337981

Following gene: 146337979

Centisome position: 12.41

GC content: 65.42

Gene sequence:

>882_bases
ATGAAGAGCGCCACGACAGCACGGATGACGGCGCCTGGAGACGTGACCAGCTTCAGCCTGGGGCTTGCACTGCCCTTGGG
CCTGGTGTTCGCGATCTTCTTCGTGCTGCCGCTGGCGCAACTGCTCTATCTGTCGCTGCACAACGACACCGCGGCGACGG
TGTGGGGGCTCGGCCAGTACATCAAGTTCCTGACCGACCCCTTCAGCCTCGCCGTGCTCGGCTCGACGCTGCTGCTCGGC
GCCGAGGTGACGCTCGCCTGTCTCGTGCTCGGCTATCCCATTGCGTGGCTGTACCAGCGCTCCGGCAGCCGCGTGCAGAC
CCTGATCATCATGATCGTGCTGCTGCCGCTGCTCACCAGCGTTGTGGTCCGCACCTTCGCCTGGATCGTCATCCTGGGCC
GGCAGGGCATCATCAACTCCGTCTTGCAGTCGCTCAGCCTGATCGGCACGCCGCTGCGCATGATCTACACCCAGGGCGGA
CTGATCGCGGCGCTGGCGCAGGTGCAGATGCCCCTGATGGTGCTGCCTTTGATCACCGCGATCGGGCGCATCGATCCGAA
TCTCTCCGACGCCTCGGCCTCGCTTGGCGCCGGCAGCTGGCGGACCTTCGTCAAGGTCATGCTGCCGCTGTCGATGCCCG
GCATCATTGCCGGCTGCACCCTCACTTACGCCGCGGCGATCACCGCCTTTATCACCCAGTCGCTGGTCGGCGGCGGGCAG
ATGCTGTTCATGCCGATGTACCTCTACCAGCAAGCCTCGACCTTGCAGAACTGGCCGTTCGCGGCGGCGATCTCGATCAT
CTTCCTGTTCGCAGTGCTCGCGATCGTCGCGCTGTTCTCGACGCTCGGCCGGCGCGCCCGCGGCTATGGAGATGCGCGAT
GA

Upstream 100 bases:

>100_bases
GAGCTGAAGAAGAAGTTCGTGTTCCAGGACTGGAAGAAGATCAACGAGCAGCGCGCGTCGTGGATCGAGAAGTTCAACCG
CGACATCAAGATCTGACGCG

Downstream 100 bases:

>100_bases
GCAAGCGGCAGCGCAACTGGGAGGCGCTCAGCTTCCAGATCGTGATGACGGTGATTGCGGTGCTGGCGCTGATCCTGATC
GTCTCGCCGTCGCTGGTCGT

Product: ABC transporter permease

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MKSATTARMTAPGDVTSFSLGLALPLGLVFAIFFVLPLAQLLYLSLHNDTAATVWGLGQYIKFLTDPFSLAVLGSTLLLG
AEVTLACLVLGYPIAWLYQRSGSRVQTLIIMIVLLPLLTSVVVRTFAWIVILGRQGIINSVLQSLSLIGTPLRMIYTQGG
LIAALAQVQMPLMVLPLITAIGRIDPNLSDASASLGAGSWRTFVKVMLPLSMPGIIAGCTLTYAAAITAFITQSLVGGGQ
MLFMPMYLYQQASTLQNWPFAAAISIIFLFAVLAIVALFSTLGRRARGYGDAR

Sequences:

>Translated_293_residues
MKSATTARMTAPGDVTSFSLGLALPLGLVFAIFFVLPLAQLLYLSLHNDTAATVWGLGQYIKFLTDPFSLAVLGSTLLLG
AEVTLACLVLGYPIAWLYQRSGSRVQTLIIMIVLLPLLTSVVVRTFAWIVILGRQGIINSVLQSLSLIGTPLRMIYTQGG
LIAALAQVQMPLMVLPLITAIGRIDPNLSDASASLGAGSWRTFVKVMLPLSMPGIIAGCTLTYAAAITAFITQSLVGGGQ
MLFMPMYLYQQASTLQNWPFAAAISIIFLFAVLAIVALFSTLGRRARGYGDAR
>Mature_293_residues
MKSATTARMTAPGDVTSFSLGLALPLGLVFAIFFVLPLAQLLYLSLHNDTAATVWGLGQYIKFLTDPFSLAVLGSTLLLG
AEVTLACLVLGYPIAWLYQRSGSRVQTLIIMIVLLPLLTSVVVRTFAWIVILGRQGIINSVLQSLSLIGTPLRMIYTQGG
LIAALAQVQMPLMVLPLITAIGRIDPNLSDASASLGAGSWRTFVKVMLPLSMPGIIAGCTLTYAAAITAFITQSLVGGGQ
MLFMPMYLYQQASTLQNWPFAAAISIIFLFAVLAIVALFSTLGRRARGYGDAR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=224, Percent_Identity=35.7142857142857, Blast_Score=148, Evalue=4e-37,
Organism=Escherichia coli, GI1787080, Length=233, Percent_Identity=32.1888412017167, Blast_Score=141, Evalue=5e-35,
Organism=Escherichia coli, GI1787713, Length=237, Percent_Identity=31.2236286919831, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1786980, Length=167, Percent_Identity=34.7305389221557, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31305; Mature: 31305

Theoretical pI: Translated: 10.11; Mature: 10.11

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSATTARMTAPGDVTSFSLGLALPLGLVFAIFFVLPLAQLLYLSLHNDTAATVWGLGQY
CCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
IKFLTDPFSLAVLGSTLLLGAEVTLACLVLGYPIAWLYQRSGSRVQTLIIMIVLLPLLTS
HHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
VVVRTFAWIVILGRQGIINSVLQSLSLIGTPLRMIYTQGGLIAALAQVQMPLMVLPLITA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
IGRIDPNLSDASASLGAGSWRTFVKVMLPLSMPGIIAGCTLTYAAAITAFITQSLVGGGQ
HHCCCCCCCCHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCH
MLFMPMYLYQQASTLQNWPFAAAISIIFLFAVLAIVALFSTLGRRARGYGDAR
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKSATTARMTAPGDVTSFSLGLALPLGLVFAIFFVLPLAQLLYLSLHNDTAATVWGLGQY
CCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
IKFLTDPFSLAVLGSTLLLGAEVTLACLVLGYPIAWLYQRSGSRVQTLIIMIVLLPLLTS
HHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
VVVRTFAWIVILGRQGIINSVLQSLSLIGTPLRMIYTQGGLIAALAQVQMPLMVLPLITA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
IGRIDPNLSDASASLGAGSWRTFVKVMLPLSMPGIIAGCTLTYAAAITAFITQSLVGGGQ
HHCCCCCCCCHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCH
MLFMPMYLYQQASTLQNWPFAAAISIIFLFAVLAIVALFSTLGRRARGYGDAR
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]