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The map label for this gene is dut

Identifier: 146337251

GI number: 146337251

Start: 88564

End: 89022

Strand: Direct

Name: dut

Synonym: BRADO0078

Alternate gene names: 146337251

Gene position: 88564-89022 (Clockwise)

Preceding gene: 146337250

Following gene: 146337252

Centisome position: 1.19

GC content: 70.81

Gene sequence:

>459_bases
GTGACACAGACGATCGCGATCGACGTTCGCATCCTGCCGCATGGCGCGGGCCTGCCGCTGCCGGCCTATCAGACCGCGCA
TGCCGCCGGGATGGACCTGCTCGCCGCCGTCGCGCCCGATGCGCCTGTCGTCCTCGCGCCAGGCAGCCACGCCATGGTGC
CAACGGGTTTGTCGATCGCGCTGCCGGACGGCTTCGAGGCGCAGGTGCGGCCGCGCTCCGGACTCGCTGCGCGGCACGGC
GTCACTGTGCTCAATTCGCCCGGCACCATCGACGCCGACTATCGCGGCGAGGTCTGCGTGCTCCTGATCAACCACGGCAA
GGAGCCGTTTACGATCCAGCGCGGCGAGCGCATCGCGCAGATGGTGATCGCGTCGGTCGTGCGCGCCGAGCTCGCGATCA
CCACGACCTTGTCGGAGACCGCGCGCGGCAGCGGCGGCTTCGGCTCGACAGGCCGGTGA

Upstream 100 bases:

>100_bases
ACGCCGACGGCGTCCATGTCGCGTCGTGGCCGATCATGACCAAGGACGAGGTGGCGAACGCGCTCGTCGCCGAGATCGCC
CGAACCGTAGGACAATCAGC

Downstream 100 bases:

>100_bases
ACTACGGGACTTCGCCACAGTTCCGACTCCGCTATGCCCATCGATGAACGCATTTTCGGTCGGTGCAACCATCGTTCACG
GTCTGGACTCTTACCGCTGG

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase

Number of amino acids: Translated: 152; Mature: 151

Protein sequence:

>152_residues
MTQTIAIDVRILPHGAGLPLPAYQTAHAAGMDLLAAVAPDAPVVLAPGSHAMVPTGLSIALPDGFEAQVRPRSGLAARHG
VTVLNSPGTIDADYRGEVCVLLINHGKEPFTIQRGERIAQMVIASVVRAELAITTTLSETARGSGGFGSTGR

Sequences:

>Translated_152_residues
MTQTIAIDVRILPHGAGLPLPAYQTAHAAGMDLLAAVAPDAPVVLAPGSHAMVPTGLSIALPDGFEAQVRPRSGLAARHG
VTVLNSPGTIDADYRGEVCVLLINHGKEPFTIQRGERIAQMVIASVVRAELAITTTLSETARGSGGFGSTGR
>Mature_151_residues
TQTIAIDVRILPHGAGLPLPAYQTAHAAGMDLLAAVAPDAPVVLAPGSHAMVPTGLSIALPDGFEAQVRPRSGLAARHGV
TVLNSPGTIDADYRGEVCVLLINHGKEPFTIQRGERIAQMVIASVVRAELAITTTLSETARGSGGFGSTGR

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family

Homologues:

Organism=Homo sapiens, GI70906444, Length=132, Percent_Identity=46.2121212121212, Blast_Score=112, Evalue=2e-25,
Organism=Homo sapiens, GI4503423, Length=132, Percent_Identity=46.2121212121212, Blast_Score=111, Evalue=3e-25,
Organism=Homo sapiens, GI70906441, Length=132, Percent_Identity=45.4545454545455, Blast_Score=109, Evalue=1e-24,
Organism=Escherichia coli, GI1790071, Length=149, Percent_Identity=46.3087248322148, Blast_Score=125, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI71988561, Length=151, Percent_Identity=40.3973509933775, Blast_Score=112, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6319729, Length=153, Percent_Identity=37.2549019607843, Blast_Score=101, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24583610, Length=131, Percent_Identity=38.9312977099237, Blast_Score=94, Evalue=4e-20,
Organism=Drosophila melanogaster, GI19921126, Length=131, Percent_Identity=38.9312977099237, Blast_Score=94, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUT_BRASO (A4YJH3)

Other databases:

- EMBL:   CU234118
- RefSeq:   YP_001202299.1
- ProteinModelPortal:   A4YJH3
- SMR:   A4YJH3
- STRING:   A4YJH3
- GeneID:   5115935
- GenomeReviews:   CU234118_GR
- KEGG:   bra:BRADO0078
- eggNOG:   COG0756
- HOGENOM:   HBG436079
- OMA:   GTIDEGY
- ProtClustDB:   PRK00601
- BioCyc:   BSP376:BRADO0078-MONOMER
- HAMAP:   MF_00116
- InterPro:   IPR008180
- InterPro:   IPR008181
- TIGRFAMs:   TIGR00576

Pfam domain/function: PF00692 dUTPase

EC number: =3.6.1.23

Molecular weight: Translated: 15640; Mature: 15509

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: NA

Important sites: BINDING 85-85

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQTIAIDVRILPHGAGLPLPAYQTAHAAGMDLLAAVAPDAPVVLAPGSHAMVPTGLSIA
CCCEEEEEEEEEECCCCCCCCCCCCHHHCCHHHEECCCCCCCEEECCCCCCCCCCCCEEE
LPDGFEAQVRPRSGLAARHGVTVLNSPGTIDADYRGEVCVLLINHGKEPFTIQRGERIAQ
CCCCCCEEECCCCCCCCCCCEEEECCCCCCCCCCCCCEEEEEEECCCCCEEECCHHHHHH
MVIASVVRAELAITTTLSETARGSGGFGSTGR
HHHHHHHHHHHEEEEEHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TQTIAIDVRILPHGAGLPLPAYQTAHAAGMDLLAAVAPDAPVVLAPGSHAMVPTGLSIA
CCEEEEEEEEEECCCCCCCCCCCCHHHCCHHHEECCCCCCCEEECCCCCCCCCCCCEEE
LPDGFEAQVRPRSGLAARHGVTVLNSPGTIDADYRGEVCVLLINHGKEPFTIQRGERIAQ
CCCCCCEEECCCCCCCCCCCEEEECCCCCCCCCCCCCEEEEEEECCCCCEEECCHHHHHH
MVIASVVRAELAITTTLSETARGSGGFGSTGR
HHHHHHHHHHHEEEEEHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA