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The map label for this gene is miaB

Identifier: 146337215

GI number: 146337215

Start: 46538

End: 47941

Strand: Direct

Name: miaB

Synonym: BRADO0041

Alternate gene names: 146337215

Gene position: 46538-47941 (Clockwise)

Preceding gene: 146337214

Following gene: 146337216

Centisome position: 0.62

GC content: 65.88

Gene sequence:

>1404_bases
ATGACGCCGCCGCGCAAGCTGCACATCAAATCCTATGGCTGCCAGATGAATGTCTACGATGCCCAGCGCATGGTGGACAC
GCTGGGGGCTGAGGGATTCGTCGAAACGGCCGACGCGGGCGACGCCGATCTCGTCATCCTCAACACCTGCCACATCCGCG
AGAAGGCCTCCGAAAAGGTCTATTCGGAGCTGGGCCGGCTGCGGGTGGCGAAAGAGGAGGCCGCCCGGTCGGGCCGCGCG
ATGCAGATCGCGGTCGCCGGCTGCGTGGCCCAGGCCGAGGGTGTGGAAATCATCTCCCGCGCGCCCACCGTCGACGTCGT
GGTCGGGCCGCAGAGCTATCACCATCTGCCGCAGCTGCTGGCGCAGGCCAGCCGTGGCGAGCGCGCGATCGAGACCGAGT
TTCCCGCCGAGGACAAGTTTGGTTTCCTCGCCAAACCCAGCCGCGAGGCGATACGCGCCCGCGGCGTGTCGGCTTTCGTC
ACGGTGCAGGAGGGTTGCGACAAGTTCTGCACCTTCTGCGTGGTGCCGTACACACGCGGCGCCGAAATGTCGCGCCCGGT
GGCACGGATTGTCGACGATGTCATGCAGCTCACCGACAGCGGTGTCCGCGAGATCACGCTGATCGGCCAGAACGTCAACG
CCTATCATGGCGAGGGTCCGGACGGCCGCACCTGGACGCTCGGCCGGCTGCTCTACCGGATCGCCGAGATCCCGGGTGTG
GCCCGCATCCGCTATTCCACCAGCCATCCGAATGACGTCGACGACGGCCTGATCGCCGCGCATCGCGACCTCACCGCCGT
CATGCCGTTTGTGCATCTTCCGGTGCAGTCGGGATCCGACCGGATCCTCGCGGCCATGAACCGGAAGCACAGCGCGGCCG
ACTATCGGCGGGTTGTCGACCGTTTCCGCGGCGCGCGGGACGACATTGCATTCTCGTCCGACTTCATCGTCGGGTTCCCC
GGCGAGACCGAGGAAGATTTTCGCGCCACCCTCGCGCTGATCGATCAAATCGGTTACGCTGCGGCCTATTCTTTCAAGTA
TTCGCCGCGCCCGGGAACGCCGGCGGCGGACATGCAGGAGATGGTGTCAGCGACCGAGATGGACGAGCGATTGGAGCGCC
TCCAGAGCTTGATCGACAGCCAGCAGGCAGCCTTCAACAAGGCCGCGATTGGCTCGGTCGTCGACGTGTTGTTCGAGCGC
GAGGCCCGCAAGCCGGGTCAGCTGGTCGGACGCACGGCCTATCTGCAGCCAGCCCATGTGATGGCGTCCAGCGACATCAT
CGGTCAGGTTCTGCCGGTCAGGATCGACAGTCTCGAGCGCTACAGCCTGCTCGGCGAGCTCGTGGCGACGACAGCGGCTC
CGATTCCCGAAGCCGCCATGCCCCTCGCGTCCATCGGAGGCTGA

Upstream 100 bases:

>100_bases
CTGGCTGAGAAAACGGGGCCACTCGCTGGATTTTCGGCGGTCTAGCCTATATCTGAGGCCCGCGCCGAGTTCGGCGCGCC
TCCGACCGCTGCAAGGGTCC

Downstream 100 bases:

>100_bases
GCACTTGCCCAAGAGCGCATCGGACTCATCTTCCCTCGCTCCCAGCCGCAAGTTCGACCGCGACATGTCCATGCCCCCCG
AAACCCAGGTCGTCATCGAT

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MTPPRKLHIKSYGCQMNVYDAQRMVDTLGAEGFVETADAGDADLVILNTCHIREKASEKVYSELGRLRVAKEEAARSGRA
MQIAVAGCVAQAEGVEIISRAPTVDVVVGPQSYHHLPQLLAQASRGERAIETEFPAEDKFGFLAKPSREAIRARGVSAFV
TVQEGCDKFCTFCVVPYTRGAEMSRPVARIVDDVMQLTDSGVREITLIGQNVNAYHGEGPDGRTWTLGRLLYRIAEIPGV
ARIRYSTSHPNDVDDGLIAAHRDLTAVMPFVHLPVQSGSDRILAAMNRKHSAADYRRVVDRFRGARDDIAFSSDFIVGFP
GETEEDFRATLALIDQIGYAAAYSFKYSPRPGTPAADMQEMVSATEMDERLERLQSLIDSQQAAFNKAAIGSVVDVLFER
EARKPGQLVGRTAYLQPAHVMASSDIIGQVLPVRIDSLERYSLLGELVATTAAPIPEAAMPLASIGG

Sequences:

>Translated_467_residues
MTPPRKLHIKSYGCQMNVYDAQRMVDTLGAEGFVETADAGDADLVILNTCHIREKASEKVYSELGRLRVAKEEAARSGRA
MQIAVAGCVAQAEGVEIISRAPTVDVVVGPQSYHHLPQLLAQASRGERAIETEFPAEDKFGFLAKPSREAIRARGVSAFV
TVQEGCDKFCTFCVVPYTRGAEMSRPVARIVDDVMQLTDSGVREITLIGQNVNAYHGEGPDGRTWTLGRLLYRIAEIPGV
ARIRYSTSHPNDVDDGLIAAHRDLTAVMPFVHLPVQSGSDRILAAMNRKHSAADYRRVVDRFRGARDDIAFSSDFIVGFP
GETEEDFRATLALIDQIGYAAAYSFKYSPRPGTPAADMQEMVSATEMDERLERLQSLIDSQQAAFNKAAIGSVVDVLFER
EARKPGQLVGRTAYLQPAHVMASSDIIGQVLPVRIDSLERYSLLGELVATTAAPIPEAAMPLASIGG
>Mature_466_residues
TPPRKLHIKSYGCQMNVYDAQRMVDTLGAEGFVETADAGDADLVILNTCHIREKASEKVYSELGRLRVAKEEAARSGRAM
QIAVAGCVAQAEGVEIISRAPTVDVVVGPQSYHHLPQLLAQASRGERAIETEFPAEDKFGFLAKPSREAIRARGVSAFVT
VQEGCDKFCTFCVVPYTRGAEMSRPVARIVDDVMQLTDSGVREITLIGQNVNAYHGEGPDGRTWTLGRLLYRIAEIPGVA
RIRYSTSHPNDVDDGLIAAHRDLTAVMPFVHLPVQSGSDRILAAMNRKHSAADYRRVVDRFRGARDDIAFSSDFIVGFPG
ETEEDFRATLALIDQIGYAAAYSFKYSPRPGTPAADMQEMVSATEMDERLERLQSLIDSQQAAFNKAAIGSVVDVLFERE
ARKPGQLVGRTAYLQPAHVMASSDIIGQVLPVRIDSLERYSLLGELVATTAAPIPEAAMPLASIGG

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872782, Length=431, Percent_Identity=33.6426914153132, Blast_Score=258, Evalue=8e-69,
Organism=Homo sapiens, GI28872784, Length=431, Percent_Identity=33.6426914153132, Blast_Score=258, Evalue=8e-69,
Organism=Homo sapiens, GI93277076, Length=474, Percent_Identity=29.746835443038, Blast_Score=186, Evalue=3e-47,
Organism=Escherichia coli, GI1786882, Length=450, Percent_Identity=46, Blast_Score=397, Evalue=1e-112,
Organism=Escherichia coli, GI1787057, Length=466, Percent_Identity=27.6824034334764, Blast_Score=162, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17553146, Length=423, Percent_Identity=32.6241134751773, Blast_Score=226, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI71996771, Length=385, Percent_Identity=25.4545454545455, Blast_Score=111, Evalue=9e-25,
Organism=Drosophila melanogaster, GI21356207, Length=432, Percent_Identity=33.3333333333333, Blast_Score=246, Evalue=3e-65,
Organism=Drosophila melanogaster, GI19922432, Length=454, Percent_Identity=27.0925110132159, Blast_Score=168, Evalue=8e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_BRASO (A4YJD7)

Other databases:

- EMBL:   CU234118
- RefSeq:   YP_001202263.1
- STRING:   A4YJD7
- GeneID:   5121431
- GenomeReviews:   CU234118_GR
- KEGG:   bra:BRADO0041
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   SYHHLPQ
- ProtClustDB:   PRK14325
- BioCyc:   BSP376:BRADO0041-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 50883; Mature: 50752

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPPRKLHIKSYGCQMNVYDAQRMVDTLGAEGFVETADAGDADLVILNTCHIREKASEKV
CCCCCEEEEEECCCEEEHHHHHHHHHHHCCCCCEECCCCCCCCEEEEEEHHHHHHHHHHH
YSELGRLRVAKEEAARSGRAMQIAVAGCVAQAEGVEIISRAPTVDVVVGPQSYHHLPQLL
HHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHCCHHHHHCCCEEEEEECCHHHHHHHHHH
AQASRGERAIETEFPAEDKFGFLAKPSREAIRARGVSAFVTVQEGCDKFCTFCVVPYTRG
HHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEEEHHHHHHHHHHEEECCCCCC
AEMSRPVARIVDDVMQLTDSGVREITLIGQNVNAYHGEGPDGRTWTLGRLLYRIAEIPGV
CCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCEEHHHHHHHHHHHCCCC
ARIRYSTSHPNDVDDGLIAAHRDLTAVMPFVHLPVQSGSDRILAAMNRKHSAADYRRVVD
EEEEECCCCCCCCCCCHHHHHHHHHHHHHHHEECCCCCCCHHHHHHCCCCCHHHHHHHHH
RFRGARDDIAFSSDFIVGFPGETEEDFRATLALIDQIGYAAAYSFKYSPRPGTPAADMQE
HHCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCHHHHHH
MVSATEMDERLERLQSLIDSQQAAFNKAAIGSVVDVLFEREARKPGQLVGRTAYLQPAHV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHCCCCHHH
MASSDIIGQVLPVRIDSLERYSLLGELVATTAAPIPEAAMPLASIGG
HHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCHHHCCC
>Mature Secondary Structure 
TPPRKLHIKSYGCQMNVYDAQRMVDTLGAEGFVETADAGDADLVILNTCHIREKASEKV
CCCCEEEEEECCCEEEHHHHHHHHHHHCCCCCEECCCCCCCCEEEEEEHHHHHHHHHHH
YSELGRLRVAKEEAARSGRAMQIAVAGCVAQAEGVEIISRAPTVDVVVGPQSYHHLPQLL
HHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHCCHHHHHCCCEEEEEECCHHHHHHHHHH
AQASRGERAIETEFPAEDKFGFLAKPSREAIRARGVSAFVTVQEGCDKFCTFCVVPYTRG
HHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEEEHHHHHHHHHHEEECCCCCC
AEMSRPVARIVDDVMQLTDSGVREITLIGQNVNAYHGEGPDGRTWTLGRLLYRIAEIPGV
CCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCEEHHHHHHHHHHHCCCC
ARIRYSTSHPNDVDDGLIAAHRDLTAVMPFVHLPVQSGSDRILAAMNRKHSAADYRRVVD
EEEEECCCCCCCCCCCHHHHHHHHHHHHHHHEECCCCCCCHHHHHHCCCCCHHHHHHHHH
RFRGARDDIAFSSDFIVGFPGETEEDFRATLALIDQIGYAAAYSFKYSPRPGTPAADMQE
HHCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCHHHHHH
MVSATEMDERLERLQSLIDSQQAAFNKAAIGSVVDVLFEREARKPGQLVGRTAYLQPAHV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHCCCCHHH
MASSDIIGQVLPVRIDSLERYSLLGELVATTAAPIPEAAMPLASIGG
HHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA