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The map label for this gene is yieH [H]

Identifier: 146337214

GI number: 146337214

Start: 45763

End: 46482

Strand: Direct

Name: yieH [H]

Synonym: BRADO0040

Alternate gene names: 146337214

Gene position: 45763-46482 (Clockwise)

Preceding gene: 146337213

Following gene: 146337215

Centisome position: 0.61

GC content: 66.39

Gene sequence:

>720_bases
ATGGCTGACGCGGCTCCGCCTTCCCCGCTCGATCTCGTCATTTTTGATTGCGACGGCGTGCTGGTCGACAGCGAGGTGAT
CTCCTGTCAGGCGCACGCCGACGTGCTTAGCAGTTGCGGCTATCCGATCACTGCGAATCAGGTGTTCGACCGTTTCCTCG
GCCGTTCCTCCAAACAGGCGACGCTGGAGGTGGAGGCCGAGCTCGGCCGCAGCTTGCCCGCCGACTTCAATGCGCGGTTG
CAGGACCGGCTGTTCCGCGCCTTCGAGCAGGACCTTCATCCCGTCCCCGGAATCGTCGAGACACTCGACGCGCTTCAGCT
CCCGGTCTGCGTGGCGTCGTCCGGCTCGCATCAGCGCATGCGGGTCAGCCTCGGCGCGACCCGCCTTTATGACCGTCTTG
CCCCGCACATCTTCTCGTCGTCGCAGGTCGAGAACGGCAAGCCGGCGCCCGATCTGTTCCTGTTCGCGGCGGCCCAGATG
AGGGCTCGTCCCGCTGCCTGCGTGGTGGTCGAGGACAGCCTCGCCGGTATCCGGGGCGGCGTCGCCGCCGGAATGACCGT
GCTCGGGTTCCATGGCGGCAGTCATTGCCGGCCCGGTCACGCGGAAAGCCTGCGCGCGGCCGGCGCAGAGCTGGTGTTCG
ACGATATGCGCCAGCTGCCAATGATTCTCAGCCGGCTGGCTGAGAAAACGGGGCCACTCGCTGGATTTTCGGCGGTCTAG

Upstream 100 bases:

>100_bases
GAGATCGAGAAGCTGCAGGCCGAGATCGCCCGCAGGCTCGGCTACAAGCTGGTCGACCACCGGCTCGAGCTGTATTGCGT
GCCGCTCGACGAAGAGACGA

Downstream 100 bases:

>100_bases
CCTATATCTGAGGCCCGCGCCGAGTTCGGCGCGCCTCCGACCGCTGCAAGGGTCCATGACGCCGCCGCGCAAGCTGCACA
TCAAATCCTATGGCTGCCAG

Product: putative haloacid dehalogenase-like hydrolase phosphatase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 239; Mature: 238

Protein sequence:

>239_residues
MADAAPPSPLDLVIFDCDGVLVDSEVISCQAHADVLSSCGYPITANQVFDRFLGRSSKQATLEVEAELGRSLPADFNARL
QDRLFRAFEQDLHPVPGIVETLDALQLPVCVASSGSHQRMRVSLGATRLYDRLAPHIFSSSQVENGKPAPDLFLFAAAQM
RARPAACVVVEDSLAGIRGGVAAGMTVLGFHGGSHCRPGHAESLRAAGAELVFDDMRQLPMILSRLAEKTGPLAGFSAV

Sequences:

>Translated_239_residues
MADAAPPSPLDLVIFDCDGVLVDSEVISCQAHADVLSSCGYPITANQVFDRFLGRSSKQATLEVEAELGRSLPADFNARL
QDRLFRAFEQDLHPVPGIVETLDALQLPVCVASSGSHQRMRVSLGATRLYDRLAPHIFSSSQVENGKPAPDLFLFAAAQM
RARPAACVVVEDSLAGIRGGVAAGMTVLGFHGGSHCRPGHAESLRAAGAELVFDDMRQLPMILSRLAEKTGPLAGFSAV
>Mature_238_residues
ADAAPPSPLDLVIFDCDGVLVDSEVISCQAHADVLSSCGYPITANQVFDRFLGRSSKQATLEVEAELGRSLPADFNARLQ
DRLFRAFEQDLHPVPGIVETLDALQLPVCVASSGSHQRMRVSLGATRLYDRLAPHIFSSSQVENGKPAPDLFLFAAAQMR
ARPAACVVVEDSLAGIRGGVAAGMTVLGFHGGSHCRPGHAESLRAAGAELVFDDMRQLPMILSRLAEKTGPLAGFSAV

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrates phosphoenolpyruvate and AMP [H]

COG id: COG0637

COG function: function code R; Predicted phosphatase/phosphohexomutase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1790151, Length=192, Percent_Identity=32.2916666666667, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1787576, Length=201, Percent_Identity=31.3432835820896, Blast_Score=64, Evalue=7e-12,
Organism=Drosophila melanogaster, GI20129151, Length=192, Percent_Identity=27.6041666666667, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI45550911, Length=191, Percent_Identity=30.8900523560209, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI116008157, Length=190, Percent_Identity=30.5263157894737, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 25395; Mature: 25264

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADAAPPSPLDLVIFDCDGVLVDSEVISCQAHADVLSSCGYPITANQVFDRFLGRSSKQA
CCCCCCCCCCEEEEECCCCEEECHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCE
TLEVEAELGRSLPADFNARLQDRLFRAFEQDLHPVPGIVETLDALQLPVCVASSGSHQRM
EEEEEHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEECCCCCCEE
RVSLGATRLYDRLAPHIFSSSQVENGKPAPDLFLFAAAQMRARPAACVVVEDSLAGIRGG
EEHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCHHHHHCC
VAAGMTVLGFHGGSHCRPGHAESLRAAGAELVFDDMRQLPMILSRLAEKTGPLAGFSAV
HHHCEEEEEECCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
ADAAPPSPLDLVIFDCDGVLVDSEVISCQAHADVLSSCGYPITANQVFDRFLGRSSKQA
CCCCCCCCCEEEEECCCCEEECHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCE
TLEVEAELGRSLPADFNARLQDRLFRAFEQDLHPVPGIVETLDALQLPVCVASSGSHQRM
EEEEEHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEECCCCCCEE
RVSLGATRLYDRLAPHIFSSSQVENGKPAPDLFLFAAAQMRARPAACVVVEDSLAGIRGG
EEHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCHHHHHCC
VAAGMTVLGFHGGSHCRPGHAESLRAAGAELVFDDMRQLPMILSRLAEKTGPLAGFSAV
HHHCEEEEEECCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]