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The map label for this gene is dnaN [H]

Identifier: 146337177

GI number: 146337177

Start: 2659

End: 3777

Strand: Direct

Name: dnaN [H]

Synonym: BRADO0002

Alternate gene names: 146337177

Gene position: 2659-3777 (Clockwise)

Preceding gene: 146337176

Following gene: 146337178

Centisome position: 0.04

GC content: 66.22

Gene sequence:

>1119_bases
ATGAAAGTAACCGTCGAACGCGCGCAGCTGCTGAAGTCGCTGGGCCATGTCCATCGCGTGGTCGAGCGCAGGAACACGAT
CCCGATCCTCGGCAATGTCCTGGTGCGCGCCGAGAATGCCAGGCTGTCGCTGCGCGCCACCGATCTCGACCTCGAAGTGA
CGGAAACGCTGGCCGCCGAGACCGCCACCGCCGGATCCACCACTGTTCCGGCGCACATGTTCTACGACATCGTGCGCAAG
CTGCCGGACGGCTCGCAGATCGTGCTGGAGAGCGACGGCGAGCGCGCGGTGCTCGCGATCCGCGCCGGCCGCTCCAAGTT
CACCCTGCAGACCTTGCCCGAGAGCGACTTCCCGGATCTCGCCGCCGGCGAGATGACGCATGCCTTCGGCGTTCCCGCCA
AGGACATCAAGAGCCTGATCGACCGCACGCAGTTCGCGATCTCCACCGAGGAGACGCGGTATTACCTCAACGGCATCTAT
CTCCACGCCGGCGGCACCGCCAAGCAGCCGACCTTGCGCGCCGTCGCCACCGACGGCCATCGCCTGGCGCAAGTCGATCT
GCCGCAGCCGTCCGGCGCCGCGGAGATGCCCGGCGTCATCGTGCCGCGCAAGACCGTCGGCGAAGTGCAGCGGCTGATCG
AGGACAATGACAGCGAGATCAAGATCGAGCTCTCGCAGGGCAAGATCCGCTTCACGTTGGGACAGGTCGTGCTGACCTCC
AAGCTGATCGACGGCACCTTCCCTGATTACGGCCGCGTCATTCCGCAGAACAACGACAAGGAGCTCGTCGTCGACAAGGC
CGACTTCGCCGCCGCCGTCGACCGCGTCTCCACCATCTCCAGCGAGCGCGGCCGCGCCGTGAAGCTCGCGCTGTCCGCCG
GCAAGCTGGTGCTGTCCGTGACCAACCCGGATTCCGGCAGCGCCACCGAAGAGCTTGAGGTCGAATACGCCTCCGACGCG
CTCGATATCGGCTTCAACTCGCGCTATCTGCTCGATATCGCCGCCCAGATCGAGGGCGAGGTCGCGGTGCTCAGGCTGGC
AGACCCGGGCTCGCCAACCCTGATCCAGGACCGCGACAACCGCAACGCGCTGTACGTGCTGATGCCGATGCGGGTGTGA

Upstream 100 bases:

>100_bases
AAAAAGCGGCCGTTCGGGCGCGAGGTGTCATCGGCCGCGGCGCTGCTCCAGCCCGCCGGCATTCCAGGACGAGCTCAGGT
GGGAACGGGCGGGTAGTGCA

Downstream 100 bases:

>100_bases
GGCGCGCTGCAGATCGATGTGTAGAGTGGGCAAAGCGCAGCGTGCCCACCACCTCCAACTAACAGGCGGCGGGCACGGCG
GCGCCATTGCTCAGCCCGAC

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MKVTVERAQLLKSLGHVHRVVERRNTIPILGNVLVRAENARLSLRATDLDLEVTETLAAETATAGSTTVPAHMFYDIVRK
LPDGSQIVLESDGERAVLAIRAGRSKFTLQTLPESDFPDLAAGEMTHAFGVPAKDIKSLIDRTQFAISTEETRYYLNGIY
LHAGGTAKQPTLRAVATDGHRLAQVDLPQPSGAAEMPGVIVPRKTVGEVQRLIEDNDSEIKIELSQGKIRFTLGQVVLTS
KLIDGTFPDYGRVIPQNNDKELVVDKADFAAAVDRVSTISSERGRAVKLALSAGKLVLSVTNPDSGSATEELEVEYASDA
LDIGFNSRYLLDIAAQIEGEVAVLRLADPGSPTLIQDRDNRNALYVLMPMRV

Sequences:

>Translated_372_residues
MKVTVERAQLLKSLGHVHRVVERRNTIPILGNVLVRAENARLSLRATDLDLEVTETLAAETATAGSTTVPAHMFYDIVRK
LPDGSQIVLESDGERAVLAIRAGRSKFTLQTLPESDFPDLAAGEMTHAFGVPAKDIKSLIDRTQFAISTEETRYYLNGIY
LHAGGTAKQPTLRAVATDGHRLAQVDLPQPSGAAEMPGVIVPRKTVGEVQRLIEDNDSEIKIELSQGKIRFTLGQVVLTS
KLIDGTFPDYGRVIPQNNDKELVVDKADFAAAVDRVSTISSERGRAVKLALSAGKLVLSVTNPDSGSATEELEVEYASDA
LDIGFNSRYLLDIAAQIEGEVAVLRLADPGSPTLIQDRDNRNALYVLMPMRV
>Mature_372_residues
MKVTVERAQLLKSLGHVHRVVERRNTIPILGNVLVRAENARLSLRATDLDLEVTETLAAETATAGSTTVPAHMFYDIVRK
LPDGSQIVLESDGERAVLAIRAGRSKFTLQTLPESDFPDLAAGEMTHAFGVPAKDIKSLIDRTQFAISTEETRYYLNGIY
LHAGGTAKQPTLRAVATDGHRLAQVDLPQPSGAAEMPGVIVPRKTVGEVQRLIEDNDSEIKIELSQGKIRFTLGQVVLTS
KLIDGTFPDYGRVIPQNNDKELVVDKADFAAAVDRVSTISSERGRAVKLALSAGKLVLSVTNPDSGSATEELEVEYASDA
LDIGFNSRYLLDIAAQIEGEVAVLRLADPGSPTLIQDRDNRNALYVLMPMRV

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=375, Percent_Identity=40.2666666666667, Blast_Score=277, Evalue=9e-76,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40407; Mature: 40407

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVTVERAQLLKSLGHVHRVVERRNTIPILGNVLVRAENARLSLRATDLDLEVTETLAAE
CEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCEEEEEEECCCEEHHHHHHHH
TATAGSTTVPAHMFYDIVRKLPDGSQIVLESDGERAVLAIRAGRSKFTLQTLPESDFPDL
CCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCEEEEEEECCCCEEEEEECCCCCCCCC
AAGEMTHAFGVPAKDIKSLIDRTQFAISTEETRYYLNGIYLHAGGTAKQPTLRAVATDGH
CCCCCHHHCCCCHHHHHHHHHHHHEEEECCCCEEEEEEEEEECCCCCCCCEEEEEECCCC
RLAQVDLPQPSGAAEMPGVIVPRKTVGEVQRLIEDNDSEIKIELSQGKIRFTLGQVVLTS
EEEEEECCCCCCCCCCCCEEECCHHHHHHHHHHCCCCCEEEEEEECCEEEEEEHHHHHHH
KLIDGTFPDYGRVIPQNNDKELVVDKADFAAAVDRVSTISSERGRAVKLALSAGKLVLSV
HHHCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHCCCCCEEEEEEECCEEEEEE
TNPDSGSATEELEVEYASDALDIGFNSRYLLDIAAQIEGEVAVLRLADPGSPTLIQDRDN
ECCCCCCCCCEEEEEECCCEEECCCCCEEEEEEEEECCCCEEEEEEECCCCCCEEEECCC
RNALYVLMPMRV
CCEEEEEEECCC
>Mature Secondary Structure
MKVTVERAQLLKSLGHVHRVVERRNTIPILGNVLVRAENARLSLRATDLDLEVTETLAAE
CEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCEEEEEEECCCEEHHHHHHHH
TATAGSTTVPAHMFYDIVRKLPDGSQIVLESDGERAVLAIRAGRSKFTLQTLPESDFPDL
CCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCEEEEEEECCCCEEEEEECCCCCCCCC
AAGEMTHAFGVPAKDIKSLIDRTQFAISTEETRYYLNGIYLHAGGTAKQPTLRAVATDGH
CCCCCHHHCCCCHHHHHHHHHHHHEEEECCCCEEEEEEEEEECCCCCCCCEEEEEECCCC
RLAQVDLPQPSGAAEMPGVIVPRKTVGEVQRLIEDNDSEIKIELSQGKIRFTLGQVVLTS
EEEEEECCCCCCCCCCCCEEECCHHHHHHHHHHCCCCCEEEEEEECCEEEEEEHHHHHHH
KLIDGTFPDYGRVIPQNNDKELVVDKADFAAAVDRVSTISSERGRAVKLALSAGKLVLSV
HHHCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHCCCCCEEEEEEECCEEEEEE
TNPDSGSATEELEVEYASDALDIGFNSRYLLDIAAQIEGEVAVLRLADPGSPTLIQDRDN
ECCCCCCCCCEEEEEECCCEEECCCCCEEEEEEEEECCCCEEEEEEECCCCCCEEEECCC
RNALYVLMPMRV
CCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9079919; 8226640 [H]