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The map label for this gene is recF [H]

Identifier: 146337178

GI number: 146337178

Start: 3998

End: 5134

Strand: Direct

Name: recF [H]

Synonym: BRADO0003

Alternate gene names: 146337178

Gene position: 3998-5134 (Clockwise)

Preceding gene: 146337177

Following gene: 146337179

Centisome position: 0.05

GC content: 70.54

Gene sequence:

>1137_bases
ATGACCCCCTCCCGCATCAATCGCCTGTCGCTGACGCATTTCCGCAGCTATCGCGCGGCTGGTGTCAGCGTGCAGGCGGA
CATGGTGGCGCTGGTCGGGGCCAATGGCGCCGGGAAGACCAATTGCCTCGAGGCGATCTCGTTCTTCGCGCCCGGCCGCG
GCCTGCGGCGCGCCACGCTCGAGGACGTCGCCGACAACCAGGGCGACGGCTCCTGGGCGATCTCGGCGGAGATCGAGGGC
GCTCTGGGACTCGCCACCTTCGGCACTGGCATCGAGCCGCCGCGGGGCGATGCCAGCACGACGCGGCGCTGCCGCATCGA
CCGCGAGCCGGTCGGATCGGCCGCGGCGTTCGGCGATCACATCCGCATGGTGTGGCTGACGCCTTCGATGGACGGGCTGT
TCATGGGCGCCGCCTCCGAGCGCCGCCGCTTCTTCGACCGGCTGGTGCTCGCGATCGACAGCGAGCATTCCAGCCGCGTC
TCGGCGCTGGAGCGCTCGTTGCGCTCGCGCAACCGGCTGCTCGAGGTGCGCAATTTCGACGACCATTGGTGTGACGCCAT
CGAGCGCGAGACCGCCGAGCTCGCGGTCGCCGTCGCCGCGAGCCGCGGCCAGACCGCGGTGAAGCTCGCCGCGATGCTGC
GCCAGCGCGGGGCCGCCTCCGCCTTCCCGTCGGCCGAGATCGCGCTCGACGGCTGGATGGAGAATGCGCTGCTCACCGAG
CCCGCGCTCGCCGTCGAGGACCGCTACCGCGCGCTGCTGCGCGACAATCGTGCGCGCGATGCTGCAGCAGGGCGTACTCT
CGACGGCCCGCATCTCACCGACCTGCACGTCATCTACGCGCCGAAGAACATGCCGGCGCGCGACGCCTCCACCGGCGAGC
AGAAGGCGCTGCTGATCGGCCTGATCCTCGCGCATGCAACCCTGGTCGCCGAGACCACCGGCATCGTGCCGATGCTGCTG
CTCGACGAGATCGTCGCCCATCTCGATCCCGGAAGGCGCACCGCGTTGTTCGCCGAGCTCGGCACGCTCGGTGCTCAGGT
GTGGCTGACGGGCGCCGACCCCGCAGCCTTCGCCGAATTGCGCGAGCTCGGCGAGATCTTCGATGTCGAAGGCGGCCGGA
TCACCGCAAGACGGTAG

Upstream 100 bases:

>100_bases
GAGCGGAGCGACGAAGAAACCCGGGCCTTGAGGCCCTTCGTTCCGAGGCAGTCCTGGATTGCTTCGCTTCGCTCGCAATG
ACGGATCGATCTCTCGTCCA

Downstream 100 bases:

>100_bases
AGGCGCAGCACTGCGGTTCCCACACGTCGCCATCGAACGCCGGACCGATAGTCCTCGCGCGAGCGAGGACGGGGCCAAAA
CGGACCCCGGCGGGATACGA

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 377

Protein sequence:

>378_residues
MTPSRINRLSLTHFRSYRAAGVSVQADMVALVGANGAGKTNCLEAISFFAPGRGLRRATLEDVADNQGDGSWAISAEIEG
ALGLATFGTGIEPPRGDASTTRRCRIDREPVGSAAAFGDHIRMVWLTPSMDGLFMGAASERRRFFDRLVLAIDSEHSSRV
SALERSLRSRNRLLEVRNFDDHWCDAIERETAELAVAVAASRGQTAVKLAAMLRQRGAASAFPSAEIALDGWMENALLTE
PALAVEDRYRALLRDNRARDAAAGRTLDGPHLTDLHVIYAPKNMPARDASTGEQKALLIGLILAHATLVAETTGIVPMLL
LDEIVAHLDPGRRTALFAELGTLGAQVWLTGADPAAFAELRELGEIFDVEGGRITARR

Sequences:

>Translated_378_residues
MTPSRINRLSLTHFRSYRAAGVSVQADMVALVGANGAGKTNCLEAISFFAPGRGLRRATLEDVADNQGDGSWAISAEIEG
ALGLATFGTGIEPPRGDASTTRRCRIDREPVGSAAAFGDHIRMVWLTPSMDGLFMGAASERRRFFDRLVLAIDSEHSSRV
SALERSLRSRNRLLEVRNFDDHWCDAIERETAELAVAVAASRGQTAVKLAAMLRQRGAASAFPSAEIALDGWMENALLTE
PALAVEDRYRALLRDNRARDAAAGRTLDGPHLTDLHVIYAPKNMPARDASTGEQKALLIGLILAHATLVAETTGIVPMLL
LDEIVAHLDPGRRTALFAELGTLGAQVWLTGADPAAFAELRELGEIFDVEGGRITARR
>Mature_377_residues
TPSRINRLSLTHFRSYRAAGVSVQADMVALVGANGAGKTNCLEAISFFAPGRGLRRATLEDVADNQGDGSWAISAEIEGA
LGLATFGTGIEPPRGDASTTRRCRIDREPVGSAAAFGDHIRMVWLTPSMDGLFMGAASERRRFFDRLVLAIDSEHSSRVS
ALERSLRSRNRLLEVRNFDDHWCDAIERETAELAVAVAASRGQTAVKLAAMLRQRGAASAFPSAEIALDGWMENALLTEP
ALAVEDRYRALLRDNRARDAAAGRTLDGPHLTDLHVIYAPKNMPARDASTGEQKALLIGLILAHATLVAETTGIVPMLLL
DEIVAHLDPGRRTALFAELGTLGAQVWLTGADPAAFAELRELGEIFDVEGGRITARR

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP [H]

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family [H]

Homologues:

Organism=Escherichia coli, GI1790135, Length=381, Percent_Identity=25.4593175853018, Blast_Score=86, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- InterPro:   IPR007406 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 40761; Mature: 40630

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS00617 RECF_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPSRINRLSLTHFRSYRAAGVSVQADMVALVGANGAGKTNCLEAISFFAPGRGLRRATL
CCCCHHHHHHHHHHHHHHHCCCEEEECEEEEEECCCCCCHHHHHHHHHHCCCCCCHHHHH
EDVADNQGDGSWAISAEIEGALGLATFGTGIEPPRGDASTTRRCRIDREPVGSAAAFGDH
HHHHCCCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCCCCHHEECCCCCCCCHHHCCCC
IRMVWLTPSMDGLFMGAASERRRFFDRLVLAIDSEHSSRVSALERSLRSRNRLLEVRNFD
EEEEEECCCCCCEEECCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEEEECCCC
DHWCDAIERETAELAVAVAASRGQTAVKLAAMLRQRGAASAFPSAEIALDGWMENALLTE
HHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHCHHHCC
PALAVEDRYRALLRDNRARDAAAGRTLDGPHLTDLHVIYAPKNMPARDASTGEQKALLIG
CCHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHH
LILAHATLVAETTGIVPMLLLDEIVAHLDPGRRTALFAELGTLGAQVWLTGADPAAFAEL
HHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEEECCCHHHHHHH
RELGEIFDVEGGRITARR
HHHHHHHCCCCCEEECCC
>Mature Secondary Structure 
TPSRINRLSLTHFRSYRAAGVSVQADMVALVGANGAGKTNCLEAISFFAPGRGLRRATL
CCCHHHHHHHHHHHHHHHCCCEEEECEEEEEECCCCCCHHHHHHHHHHCCCCCCHHHHH
EDVADNQGDGSWAISAEIEGALGLATFGTGIEPPRGDASTTRRCRIDREPVGSAAAFGDH
HHHHCCCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCCCCHHEECCCCCCCCHHHCCCC
IRMVWLTPSMDGLFMGAASERRRFFDRLVLAIDSEHSSRVSALERSLRSRNRLLEVRNFD
EEEEEECCCCCCEEECCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEEEECCCC
DHWCDAIERETAELAVAVAASRGQTAVKLAAMLRQRGAASAFPSAEIALDGWMENALLTE
HHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHCHHHCC
PALAVEDRYRALLRDNRARDAAAGRTLDGPHLTDLHVIYAPKNMPARDASTGEQKALLIG
CCHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHH
LILAHATLVAETTGIVPMLLLDEIVAHLDPGRRTALFAELGTLGAQVWLTGADPAAFAEL
HHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEEECCCHHHHHHH
RELGEIFDVEGGRITARR
HHHHHHHCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]