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The map label for this gene is purB [H]

Identifier: 146302742

GI number: 146302742

Start: 6052158

End: 6053504

Strand: Direct

Name: purB [H]

Synonym: Fjoh_5015

Alternate gene names: 146302742

Gene position: 6052158-6053504 (Clockwise)

Preceding gene: 146302741

Following gene: 146302743

Centisome position: 99.27

GC content: 34.22

Gene sequence:

>1347_bases
ATGACTACTCTAAACGAATTGAATGCTATATCGCCAATTGACGGAAGATATAGAAATAAAACTCAAAATTTAGCACCTTT
CTTTTCTGAAGAAGCTTTAATAAAATACCGTGTATTAGTTGAAATTGAATATTTCATCGCTTTATGCGAAATTCCTTTAC
CACAGCTTTCTGGCGTAAATTCAGATTTATTCGACAGTTTGAGAAACATCTACAAAAATTTCTCTACTGAAGATGCGCTT
TGGATTAAAGAAACAGAAAAAGTAACCAACCACGACGTAAAAGCGGTTGAATACTTTATTAAAGATGCTTTCGAAAAATT
AGGTTTGTCTCAATACAAAGAGTTCATTCACTTCGGATTAACATCTCAGGATATTAACAATACTGCAATTCCTCTTTCTA
CAAAAGAAGCTTTTGAACAAGTTTATATGCCGTCTTTAGTAGCTTTAATTGCTAAACTAAAAGAATTAAGCGTTGAATGG
AAAGATATTCCGATGCTTGCCCGTACACATGGACAACCGGCTTCTCCTACTCGTTTAGGAAAAGAAATTCTTGTTTTTGT
AGAGCGTTTAGAAGAGCAGATGCGTTTGTTATTCAATATTCCGTTTGCTGCTAAATTTGGCGGTGCAACCGGAAACTTTA
ATGCACACCATGTTGCATACCCGCAGATTGACTGGAAAAAATTTGGTGATAAATTTGTTGAAACTGATTTAGGCTTACAC
CACTCTTTTCCAACAACTCAAATTGAGCATTACGATCATTTTGCTGCTTTTTTTGATGGTTTAAAAAGGATCAACAATAT
CATTATCGATTTAGACCGTGATATCTGGACGTATGTTTCAATGGATTATTTTAAACAAAAAATCAAAGCAGGAGAAATTG
GTTCATCTGCAATGCCTCATAAAGTAAACCCAATTGATTTTGAAAATTCTGAAGGAAACTTAGGAATTGCTAATGCTATT
TTCGAACATTTAGCGGCTAAACTTCCAATTTCAAGATTACAGCGTGATTTAACTGACAGTACAGTTTTGCGTAATGTTGG
TGTACCGGTTGGACATACTATTATTGCTTTTGAAGCTACTCTTAAAGGTTTAAACAAATTACTTTTAAACGAAAGCAAGT
TTGCTGAAGACTTAGAAAAAAACTGGGCAGTTGTGGCAGAAGCTATTCAAACAATCTTACGTCGTGAAGCTTATCCAAAC
CCATACGAAGCTCTAAAAGGTTTAACAAGAACTAATGAAGCAATTGATAAAAATGCGATTCATAATTTCATTGCAACTTT
AGAAGTTTCTGACGCAGTTAGAGCAGAATTAATGCAGATAACTCCTAGCAATTACACAGGAATTTAA

Upstream 100 bases:

>100_bases
CCATGATTTAAATTGAAAGTTAAGGCATAATAAAGCTTCAGTAATTTTCAGAAGTTTTAACAATCACTTATATTTGCACC
TTTCGAAAAACAACATAACA

Downstream 100 bases:

>100_bases
AGAAAACCTAGAATATTTTATCAAAAAAAGCTATCTTTATCGAGATAGCTTTTTTTATTTAACCGGAACAGTTCAGTGAA
TCTATTTGAGATTAAAAACT

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MTTLNELNAISPIDGRYRNKTQNLAPFFSEEALIKYRVLVEIEYFIALCEIPLPQLSGVNSDLFDSLRNIYKNFSTEDAL
WIKETEKVTNHDVKAVEYFIKDAFEKLGLSQYKEFIHFGLTSQDINNTAIPLSTKEAFEQVYMPSLVALIAKLKELSVEW
KDIPMLARTHGQPASPTRLGKEILVFVERLEEQMRLLFNIPFAAKFGGATGNFNAHHVAYPQIDWKKFGDKFVETDLGLH
HSFPTTQIEHYDHFAAFFDGLKRINNIIIDLDRDIWTYVSMDYFKQKIKAGEIGSSAMPHKVNPIDFENSEGNLGIANAI
FEHLAAKLPISRLQRDLTDSTVLRNVGVPVGHTIIAFEATLKGLNKLLLNESKFAEDLEKNWAVVAEAIQTILRREAYPN
PYEALKGLTRTNEAIDKNAIHNFIATLEVSDAVRAELMQITPSNYTGI

Sequences:

>Translated_448_residues
MTTLNELNAISPIDGRYRNKTQNLAPFFSEEALIKYRVLVEIEYFIALCEIPLPQLSGVNSDLFDSLRNIYKNFSTEDAL
WIKETEKVTNHDVKAVEYFIKDAFEKLGLSQYKEFIHFGLTSQDINNTAIPLSTKEAFEQVYMPSLVALIAKLKELSVEW
KDIPMLARTHGQPASPTRLGKEILVFVERLEEQMRLLFNIPFAAKFGGATGNFNAHHVAYPQIDWKKFGDKFVETDLGLH
HSFPTTQIEHYDHFAAFFDGLKRINNIIIDLDRDIWTYVSMDYFKQKIKAGEIGSSAMPHKVNPIDFENSEGNLGIANAI
FEHLAAKLPISRLQRDLTDSTVLRNVGVPVGHTIIAFEATLKGLNKLLLNESKFAEDLEKNWAVVAEAIQTILRREAYPN
PYEALKGLTRTNEAIDKNAIHNFIATLEVSDAVRAELMQITPSNYTGI
>Mature_447_residues
TTLNELNAISPIDGRYRNKTQNLAPFFSEEALIKYRVLVEIEYFIALCEIPLPQLSGVNSDLFDSLRNIYKNFSTEDALW
IKETEKVTNHDVKAVEYFIKDAFEKLGLSQYKEFIHFGLTSQDINNTAIPLSTKEAFEQVYMPSLVALIAKLKELSVEWK
DIPMLARTHGQPASPTRLGKEILVFVERLEEQMRLLFNIPFAAKFGGATGNFNAHHVAYPQIDWKKFGDKFVETDLGLHH
SFPTTQIEHYDHFAAFFDGLKRINNIIIDLDRDIWTYVSMDYFKQKIKAGEIGSSAMPHKVNPIDFENSEGNLGIANAIF
EHLAAKLPISRLQRDLTDSTVLRNVGVPVGHTIIAFEATLKGLNKLLLNESKFAEDLEKNWAVVAEAIQTILRREAYPNP
YEALKGLTRTNEAIDKNAIHNFIATLEVSDAVRAELMQITPSNYTGI

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787376, Length=446, Percent_Identity=48.6547085201794, Blast_Score=438, Evalue=1e-124,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013539
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF08328 ASL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 50814; Mature: 50682

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLNELNAISPIDGRYRNKTQNLAPFFSEEALIKYRVLVEIEYFIALCEIPLPQLSGVN
CCCHHHCCCCCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCC
SDLFDSLRNIYKNFSTEDALWIKETEKVTNHDVKAVEYFIKDAFEKLGLSQYKEFIHFGL
HHHHHHHHHHHHCCCCCCCEEEHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCC
TSQDINNTAIPLSTKEAFEQVYMPSLVALIAKLKELSVEWKDIPMLARTHGQPASPTRLG
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHCCCCCCCHHHHH
KEILVFVERLEEQMRLLFNIPFAAKFGGATGNFNAHHVAYPQIDWKKFGDKFVETDLGLH
HHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCCC
HSFPTTQIEHYDHFAAFFDGLKRINNIIIDLDRDIWTYVSMDYFKQKIKAGEIGSSAMPH
CCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCC
KVNPIDFENSEGNLGIANAIFEHLAAKLPISRLQRDLTDSTVLRNVGVPVGHTIIAFEAT
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
LKGLNKLLLNESKFAEDLEKNWAVVAEAIQTILRREAYPNPYEALKGLTRTNEAIDKNAI
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
HNFIATLEVSDAVRAELMQITPSNYTGI
HHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TTLNELNAISPIDGRYRNKTQNLAPFFSEEALIKYRVLVEIEYFIALCEIPLPQLSGVN
CCHHHCCCCCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCC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HHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]