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The map label for this gene is bfmBB [H]

Identifier: 146302715

GI number: 146302715

Start: 6023954

End: 6025309

Strand: Reverse

Name: bfmBB [H]

Synonym: Fjoh_4988

Alternate gene names: 146302715

Gene position: 6025309-6023954 (Counterclockwise)

Preceding gene: 146302716

Following gene: 146302714

Centisome position: 98.83

GC content: 38.64

Gene sequence:

>1356_bases
ATGGCAAGATTTGAATTAAAGCTTCCAAAAATGGGAGAGAGCGTCGCTGAGGCAACCATTACTAACTGGTTGAAAGAAGT
GGGAGACAAAATTGAAGCTGATGAAGCTGTACTGGAAATTGCAACTGATAAAGTTGACAGCGAAGTGCCAAGTGAAGTAT
CAGGAATTTTAGTTGAACAATTATTTGGCAAAGATGATTTAGTTCAGGTAGGGCAGACTATTGCAATTATCGAAACAGAA
GGTGGTGATGCGCCTGCAGTAACTCCAGTTGTCGAAGTTTCTGTACCTGCAGAAGCAGTAGAAATAGAAAAAACTATCGA
AGCAGTAAAAGAAACCGTAACTGCTCCGCAGGATTTTGCAGGATCAGATAAATTCTTTTCTCCTCTGGTAAAAAATATTG
CAAAAGAAGAAGGTATTTCTGTTGCAGAACTAGATAGTATTCAAGGTTCAGGAAAAGATGGCCGAGTAACAAAAGATGAT
ATTTTTAAATATATAGAAGACCGCAAGTCAGGGGTTGTGCAAGCTCCAAAAGCAGTTGAAGAAGCTCCAAAAGCAGTGGT
TGAAACACCAAAGGCTGTTGTTGAAACTCCAAAAGCAGAACCAGTCGCTCAAAAAAGCCAGCAGGCTGTTCCGGTTTCTG
TAAACGGTGGTGACGAAATTGTGGAAATGGACAGAATGCGTAAACTGATTTCAGGTTACATGACAGCTTCTGTACAAACT
TCGGCTCACGTTCAATCTTTTATTGAGGTTGACGTAACCAACATTGTAAAATGGAGAGATAAAGTTAAAACCGCTTTCGA
AAAAAGAGAAGGTGAAAAATTGACTTTTACACCAATTATGATGGAAGCAGTTGCAAAAGCGTTAAAAGATTTCCCTGGAA
TGAATATTTCTGTTGATGGTGATTATATCATCAAAAAGAAAAACATCAATTTAGGAATGGCAGCAGCATTGCCAAACGGA
AATTTAATTGTTCCTGTAATTAAAAATGCAGATCAGTTAAACCTTGTTGGAATGGCAAAAGCAGTTAACGATTTAGGAAA
CCGTGCAAAAGCAGGAAAACTAAAACCAGATGATACACAAGGCGGAACTTACACTGTTACAAATGTTGGAACTTTTGGAA
GTGTTTTTGGAACGCCTATTATTAATCAGCCACAGGTTGGAATTTTAGCTCTTGGTGCGATTCGTAAAGTTCCTGCGGTT
ATCGAAACTCCAGAAGGAGATTTTATCGGAATCCGTCAAAAAATGTTCCTGTCTCACTCTTACGATCATAGAGTGGTAGA
TGGCGCATTAGGAGGAAGTTTTGTGAAAAGAGTAGCAGAATATTTAGAAGCTTTTGATGTAGAAAGAGATTTCTAG

Upstream 100 bases:

>100_bases
TCATTAAAATTGAGTAAAAATTATATTAAGTGTAATTTATATTTAAAAATTTCAGTAATTTCGCAATCAGAAAATCAAAA
TCACCCTTTAGAATAATAAT

Downstream 100 bases:

>100_bases
TCGATTTAAAATAAAATTTGAAAACCCGAAAAATTTAAAAGTTTTTCGGGTTTTTTCGTTTATAAAAAATGGAGAAAAAC
AACTCGTTTTGGTTTGATTT

Product: dehydrogenase catalytic domain-containing protein

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase complex component E2; BCKAD-E2; BCKADE2; Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex; Dihydrolipoamide branched chain transacylase; Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase [H]

Number of amino acids: Translated: 451; Mature: 450

Protein sequence:

>451_residues
MARFELKLPKMGESVAEATITNWLKEVGDKIEADEAVLEIATDKVDSEVPSEVSGILVEQLFGKDDLVQVGQTIAIIETE
GGDAPAVTPVVEVSVPAEAVEIEKTIEAVKETVTAPQDFAGSDKFFSPLVKNIAKEEGISVAELDSIQGSGKDGRVTKDD
IFKYIEDRKSGVVQAPKAVEEAPKAVVETPKAVVETPKAEPVAQKSQQAVPVSVNGGDEIVEMDRMRKLISGYMTASVQT
SAHVQSFIEVDVTNIVKWRDKVKTAFEKREGEKLTFTPIMMEAVAKALKDFPGMNISVDGDYIIKKKNINLGMAAALPNG
NLIVPVIKNADQLNLVGMAKAVNDLGNRAKAGKLKPDDTQGGTYTVTNVGTFGSVFGTPIINQPQVGILALGAIRKVPAV
IETPEGDFIGIRQKMFLSHSYDHRVVDGALGGSFVKRVAEYLEAFDVERDF

Sequences:

>Translated_451_residues
MARFELKLPKMGESVAEATITNWLKEVGDKIEADEAVLEIATDKVDSEVPSEVSGILVEQLFGKDDLVQVGQTIAIIETE
GGDAPAVTPVVEVSVPAEAVEIEKTIEAVKETVTAPQDFAGSDKFFSPLVKNIAKEEGISVAELDSIQGSGKDGRVTKDD
IFKYIEDRKSGVVQAPKAVEEAPKAVVETPKAVVETPKAEPVAQKSQQAVPVSVNGGDEIVEMDRMRKLISGYMTASVQT
SAHVQSFIEVDVTNIVKWRDKVKTAFEKREGEKLTFTPIMMEAVAKALKDFPGMNISVDGDYIIKKKNINLGMAAALPNG
NLIVPVIKNADQLNLVGMAKAVNDLGNRAKAGKLKPDDTQGGTYTVTNVGTFGSVFGTPIINQPQVGILALGAIRKVPAV
IETPEGDFIGIRQKMFLSHSYDHRVVDGALGGSFVKRVAEYLEAFDVERDF
>Mature_450_residues
ARFELKLPKMGESVAEATITNWLKEVGDKIEADEAVLEIATDKVDSEVPSEVSGILVEQLFGKDDLVQVGQTIAIIETEG
GDAPAVTPVVEVSVPAEAVEIEKTIEAVKETVTAPQDFAGSDKFFSPLVKNIAKEEGISVAELDSIQGSGKDGRVTKDDI
FKYIEDRKSGVVQAPKAVEEAPKAVVETPKAVVETPKAEPVAQKSQQAVPVSVNGGDEIVEMDRMRKLISGYMTASVQTS
AHVQSFIEVDVTNIVKWRDKVKTAFEKREGEKLTFTPIMMEAVAKALKDFPGMNISVDGDYIIKKKNINLGMAAALPNGN
LIVPVIKNADQLNLVGMAKAVNDLGNRAKAGKLKPDDTQGGTYTVTNVGTFGSVFGTPIINQPQVGILALGAIRKVPAVI
ETPEGDFIGIRQKMFLSHSYDHRVVDGALGGSFVKRVAEYLEAFDVERDF

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=442, Percent_Identity=30.0904977375566, Blast_Score=175, Evalue=7e-44,
Organism=Homo sapiens, GI19923748, Length=255, Percent_Identity=33.7254901960784, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI203098753, Length=454, Percent_Identity=28.1938325991189, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI203098816, Length=454, Percent_Identity=28.1938325991189, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI31711992, Length=462, Percent_Identity=28.3549783549784, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI260898739, Length=206, Percent_Identity=32.0388349514563, Blast_Score=89, Evalue=6e-18,
Organism=Escherichia coli, GI1786946, Length=443, Percent_Identity=30.2483069977427, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI1786305, Length=450, Percent_Identity=29.3333333333333, Blast_Score=164, Evalue=8e-42,
Organism=Caenorhabditis elegans, GI17537937, Length=457, Percent_Identity=29.7592997811816, Blast_Score=166, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI25146366, Length=228, Percent_Identity=34.6491228070175, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17560088, Length=468, Percent_Identity=28.2051282051282, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17538894, Length=346, Percent_Identity=26.3005780346821, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6320352, Length=444, Percent_Identity=30.8558558558559, Blast_Score=194, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6324258, Length=467, Percent_Identity=28.051391862955, Blast_Score=132, Evalue=1e-31,
Organism=Drosophila melanogaster, GI18859875, Length=441, Percent_Identity=27.2108843537415, Blast_Score=144, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24645909, Length=223, Percent_Identity=34.9775784753363, Blast_Score=142, Evalue=6e-34,
Organism=Drosophila melanogaster, GI24582497, Length=227, Percent_Identity=30.3964757709251, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI20129315, Length=227, Percent_Identity=30.3964757709251, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.168 [H]

Molecular weight: Translated: 48556; Mature: 48425

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARFELKLPKMGESVAEATITNWLKEVGDKIEADEAVLEIATDKVDSEVPSEVSGILVEQ
CCCEEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHH
LFGKDDLVQVGQTIAIIETEGGDAPAVTPVVEVSVPAEAVEIEKTIEAVKETVTAPQDFA
HHCCHHHHHCCCEEEEEEECCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCC
GSDKFFSPLVKNIAKEEGISVAELDSIQGSGKDGRVTKDDIFKYIEDRKSGVVQAPKAVE
CCHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHH
EAPKAVVETPKAVVETPKAEPVAQKSQQAVPVSVNGGDEIVEMDRMRKLISGYMTASVQT
HHHHHHHHCCHHHHCCCCCCHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCHH
SAHVQSFIEVDVTNIVKWRDKVKTAFEKREGEKLTFTPIMMEAVAKALKDFPGMNISVDG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCCCEEEECC
DYIIKKKNINLGMAAALPNGNLIVPVIKNADQLNLVGMAKAVNDLGNRAKAGKLKPDDTQ
CEEEEECCCCEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCC
GGTYTVTNVGTFGSVFGTPIINQPQVGILALGAIRKVPAVIETPEGDFIGIRQKMFLSHS
CCEEEEEECCCHHHHHCCCCCCCCCCCCEEHHHHHHCCCEEECCCCCEEHHHHHHHHHCC
YDHRVVDGALGGSFVKRVAEYLEAFDVERDF
CCCHHHHHHCCHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
ARFELKLPKMGESVAEATITNWLKEVGDKIEADEAVLEIATDKVDSEVPSEVSGILVEQ
CCEEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHH
LFGKDDLVQVGQTIAIIETEGGDAPAVTPVVEVSVPAEAVEIEKTIEAVKETVTAPQDFA
HHCCHHHHHCCCEEEEEEECCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCC
GSDKFFSPLVKNIAKEEGISVAELDSIQGSGKDGRVTKDDIFKYIEDRKSGVVQAPKAVE
CCHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHH
EAPKAVVETPKAVVETPKAEPVAQKSQQAVPVSVNGGDEIVEMDRMRKLISGYMTASVQT
HHHHHHHHCCHHHHCCCCCCHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCHH
SAHVQSFIEVDVTNIVKWRDKVKTAFEKREGEKLTFTPIMMEAVAKALKDFPGMNISVDG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCCCEEEECC
DYIIKKKNINLGMAAALPNGNLIVPVIKNADQLNLVGMAKAVNDLGNRAKAGKLKPDDTQ
CEEEEECCCCEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCC
GGTYTVTNVGTFGSVFGTPIINQPQVGILALGAIRKVPAVIETPEGDFIGIRQKMFLSHS
CCEEEEEECCCHHHHHCCCCCCCCCCCCEEHHHHHHCCCEEECCCCCEEHHHHHHHHHCC
YDHRVVDGALGGSFVKRVAEYLEAFDVERDF
CCCHHHHHHCCHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8504804; 8969508; 9384377; 7961792 [H]