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The map label for this gene is pgdS [H]

Identifier: 146302693

GI number: 146302693

Start: 6002947

End: 6003456

Strand: Direct

Name: pgdS [H]

Synonym: Fjoh_4966

Alternate gene names: 146302693

Gene position: 6002947-6003456 (Clockwise)

Preceding gene: 146302689

Following gene: 146302696

Centisome position: 98.46

GC content: 31.37

Gene sequence:

>510_bases
ATGAGGTCATATCTTTTCACAGCTTTATTATCTATTACGCTATTTTCTTCTTTTACCGTAAAAAAGAGCGAAAAGCAGAC
TTTAATTGATAATCGAAAAATTCAAACCGAAATCAATAGAGATTCTATCATCGAATATGCTAAAAAGTATTTAGATACGC
CTTATAAATATGCCAGCAGTGATCCTAAAAAAGGATTTGACTGTTCTGGTTTTGTGAGTTATGTTTTTAAAAATTTTGGC
ATGAATCTGCCAAGAAGTTCCGGCAGTTACAAAAACTTAGGAACTGCCTTAAAACCTGAAGATTTTAAAGTTGGAGATAT
TTTAGTTTTTTACGGATATAAAGACAGAACAGTTATAGGCCATTTGGGAATAATCTGTGAAGCTGACGGAATGCATTCTA
AATTCATTCATGCTTCATCAGGAAAAGCACAAAAAGTAACTATTACCGCATTAGACACAGAACATTATACCAAACGTTTT
TATAAATGTATTAATGTTTTACCTGAATAA

Upstream 100 bases:

>100_bases
TAAAACTGTTAATTCTTACAATTAGTAACGAGGTATTCTTTTGATTTACAAAATTTAGAGTCTATCTTTATCGATTCAAT
ATTTTCTTACTTACAAAAAA

Downstream 100 bases:

>100_bases
TTTTTCAGCCACAAATTAAACAGATTAACACAGAAATTTTATAATCTCTTAAATCCTTTAATCCGTGGCAGTAGACAATA
AACTTTTTTTGAAAATTTGG

Product: NLP/P60 protein

Products: NA

Alternate protein names: Poly-gamma-glutamate depolymerase; PGA depolymerase [H]

Number of amino acids: Translated: 169; Mature: 169

Protein sequence:

>169_residues
MRSYLFTALLSITLFSSFTVKKSEKQTLIDNRKIQTEINRDSIIEYAKKYLDTPYKYASSDPKKGFDCSGFVSYVFKNFG
MNLPRSSGSYKNLGTALKPEDFKVGDILVFYGYKDRTVIGHLGIICEADGMHSKFIHASSGKAQKVTITALDTEHYTKRF
YKCINVLPE

Sequences:

>Translated_169_residues
MRSYLFTALLSITLFSSFTVKKSEKQTLIDNRKIQTEINRDSIIEYAKKYLDTPYKYASSDPKKGFDCSGFVSYVFKNFG
MNLPRSSGSYKNLGTALKPEDFKVGDILVFYGYKDRTVIGHLGIICEADGMHSKFIHASSGKAQKVTITALDTEHYTKRF
YKCINVLPE
>Mature_169_residues
MRSYLFTALLSITLFSSFTVKKSEKQTLIDNRKIQTEINRDSIIEYAKKYLDTPYKYASSDPKKGFDCSGFVSYVFKNFG
MNLPRSSGSYKNLGTALKPEDFKVGDILVFYGYKDRTVIGHLGIICEADGMHSKFIHASSGKAQKVTITALDTEHYTKRF
YKCINVLPE

Specific function: Cleaves, in an endo-type manner, the gamma-glutamyl bond between D-glutamate and L-glutamate of poly-gamma-glutamate (PGA) [H]

COG id: COG0791

COG function: function code M; Cell wall-associated hydrolases (invasion-associated proteins)

Gene ontology:

Cell location: Secreted. Secreted, cell wall. Note=Cell wall localization shown in PubMed:11987133 [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nlpC/p60 family [H]

Homologues:

Organism=Escherichia coli, GI1787944, Length=145, Percent_Identity=30.3448275862069, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000064 [H]

Pfam domain/function: PF00877 NLPC_P60 [H]

EC number: NA

Molecular weight: Translated: 19203; Mature: 19203

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSYLFTALLSITLFSSFTVKKSEKQTLIDNRKIQTEINRDSIIEYAKKYLDTPYKYASS
CCHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCHHEECCCHHHHHHHHHHHHCCCCCCCCC
DPKKGFDCSGFVSYVFKNFGMNLPRSSGSYKNLGTALKPEDFKVGDILVFYGYKDRTVIG
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEE
HLGIICEADGMHSKFIHASSGKAQKVTITALDTEHYTKRFYKCINVLPE
EEEEEEECCCCCCCEEECCCCCCEEEEEEEECCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRSYLFTALLSITLFSSFTVKKSEKQTLIDNRKIQTEINRDSIIEYAKKYLDTPYKYASS
CCHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCHHEECCCHHHHHHHHHHHHCCCCCCCCC
DPKKGFDCSGFVSYVFKNFGMNLPRSSGSYKNLGTALKPEDFKVGDILVFYGYKDRTVIG
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEE
HLGIICEADGMHSKFIHASSGKAQKVTITALDTEHYTKRFYKCINVLPE
EEEEEEECCCCCCCEEECCCCCCEEEEEEEECCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12073126; 12644511; 9353933; 9384377; 10658653 [H]