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The map label for this gene is amiD [H]

Identifier: 146302671

GI number: 146302671

Start: 5971726

End: 5972631

Strand: Direct

Name: amiD [H]

Synonym: Fjoh_4944

Alternate gene names: 146302671

Gene position: 5971726-5972631 (Clockwise)

Preceding gene: 146302670

Following gene: 146302680

Centisome position: 97.95

GC content: 32.89

Gene sequence:

>906_bases
ATGGTAAGAAAACTTTTTTGTTATCTGATCTTAGCAATTATCATAACTTCTTGCTCAACGAATCCATATAAGACTACTGA
AAAGGCATACGACCAGCAGTTAAAGACTTTAGAAAATCAAATTACAAGCAAAGAAGCTCAGGCAATACCGTCTATAAGTC
CGGTTATTATCGACACTACTTATGCATCGCAGCTTGGTATTATAAAAGATACTTTATCAAAAACAGGTTCTACTTCTTTA
TTAAACGGAATTAGTACAGAATGGATTGGTACTGTAAACTTTAATTTAAGAAAACCAAGTTTTGTAATTATTCATCATAC
TGCACAGGATTCTTTGCAGCAAACTATCAATACATTTACAAAAACAAGAACTCAGGTAAGTTCTCACTATATTATTTCTG
AAAATGGAAAAGTAGTTCAAATGCTGAATGATTATTTAAGAGCATGGCACGCAGGAAATTCAACTTGGGGAAAAAACACT
GATTTGAATTCTTCATCTATAGGAATTGAACTTGATAATAATGGTTTTAAACCTTTTACAGAAGCTCAGATAAGCAGTTT
GGTTGCTTTGTTGACAAAACTAAAAAAAGATTACAACATACCAACTCAAAACTTTTTAGGTCATGCTGATATTGCACCAG
GAAGAAAACAAGATCCAAGTGCTTTATTTCCGTGGAAAACATTAGCCGAAAAAGGTTTCGGAATCTGGCCTGACGCTGTT
CTTGAAGAAGCACCATTTGATTTTAAAATTGAACCTGCATTAAGAATTATTGGTTATAACACTAAGAATCTTTCGGCAGC
AATTCAGGCTTTTAAACTGCATTATATTCAAACCGACACAACTGCAACGCTTGACCGCAAAACAATTGATACGATATATT
CTATATATAAAAAACAGATTCAGTAA

Upstream 100 bases:

>100_bases
AAATCTAAGAAGAAAAAAGATAAAGACAATAAAGAAGAAGAAAAACCAGCTGAAAAAATTCAGGAATAATTGTTCCAAAC
TTCATAAAATGTAAACTATC

Downstream 100 bases:

>100_bases
AGACATCAATTAATAAATATATAAACGCATTTCTAATTTTATAGGAATGCGTTTTTTTTTTTAGCCGCAGATTAAGAGGA
TTATAAAGATTATTATTCTC

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MVRKLFCYLILAIIITSCSTNPYKTTEKAYDQQLKTLENQITSKEAQAIPSISPVIIDTTYASQLGIIKDTLSKTGSTSL
LNGISTEWIGTVNFNLRKPSFVIIHHTAQDSLQQTINTFTKTRTQVSSHYIISENGKVVQMLNDYLRAWHAGNSTWGKNT
DLNSSSIGIELDNNGFKPFTEAQISSLVALLTKLKKDYNIPTQNFLGHADIAPGRKQDPSALFPWKTLAEKGFGIWPDAV
LEEAPFDFKIEPALRIIGYNTKNLSAAIQAFKLHYIQTDTTATLDRKTIDTIYSIYKKQIQ

Sequences:

>Translated_301_residues
MVRKLFCYLILAIIITSCSTNPYKTTEKAYDQQLKTLENQITSKEAQAIPSISPVIIDTTYASQLGIIKDTLSKTGSTSL
LNGISTEWIGTVNFNLRKPSFVIIHHTAQDSLQQTINTFTKTRTQVSSHYIISENGKVVQMLNDYLRAWHAGNSTWGKNT
DLNSSSIGIELDNNGFKPFTEAQISSLVALLTKLKKDYNIPTQNFLGHADIAPGRKQDPSALFPWKTLAEKGFGIWPDAV
LEEAPFDFKIEPALRIIGYNTKNLSAAIQAFKLHYIQTDTTATLDRKTIDTIYSIYKKQIQ
>Mature_301_residues
MVRKLFCYLILAIIITSCSTNPYKTTEKAYDQQLKTLENQITSKEAQAIPSISPVIIDTTYASQLGIIKDTLSKTGSTSL
LNGISTEWIGTVNFNLRKPSFVIIHHTAQDSLQQTINTFTKTRTQVSSHYIISENGKVVQMLNDYLRAWHAGNSTWGKNT
DLNSSSIGIELDNNGFKPFTEAQISSLVALLTKLKKDYNIPTQNFLGHADIAPGRKQDPSALFPWKTLAEKGFGIWPDAV
LEEAPFDFKIEPALRIIGYNTKNLSAAIQAFKLHYIQTDTTATLDRKTIDTIYSIYKKQIQ

Specific function: Unknown

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787092, Length=154, Percent_Identity=44.1558441558442, Blast_Score=124, Evalue=7e-30,
Organism=Escherichia coli, GI1786300, Length=101, Percent_Identity=40.5940594059406, Blast_Score=79, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 33700; Mature: 33700

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRKLFCYLILAIIITSCSTNPYKTTEKAYDQQLKTLENQITSKEAQAIPSISPVIIDTT
CHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECC
YASQLGIIKDTLSKTGSTSLLNGISTEWIGTVNFNLRKPSFVIIHHTAQDSLQQTINTFT
HHHHHHHHHHHHHCCCCHHHHHCCCCCEEEEEEEEECCCCEEEEECCHHHHHHHHHHHHH
KTRTQVSSHYIISENGKVVQMLNDYLRAWHAGNSTWGKNTDLNSSSIGIELDNNGFKPFT
HHHHHHHCEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCHH
EAQISSLVALLTKLKKDYNIPTQNFLGHADIAPGRKQDPSALFPWKTLAEKGFGIWPDAV
HHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCHHH
LEEAPFDFKIEPALRIIGYNTKNLSAAIQAFKLHYIQTDTTATLDRKTIDTIYSIYKKQI
HHCCCCCEEECCEEEEEECCCHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHC
Q
C
>Mature Secondary Structure
MVRKLFCYLILAIIITSCSTNPYKTTEKAYDQQLKTLENQITSKEAQAIPSISPVIIDTT
CHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECC
YASQLGIIKDTLSKTGSTSLLNGISTEWIGTVNFNLRKPSFVIIHHTAQDSLQQTINTFT
HHHHHHHHHHHHHCCCCHHHHHCCCCCEEEEEEEEECCCCEEEEECCHHHHHHHHHHHHH
KTRTQVSSHYIISENGKVVQMLNDYLRAWHAGNSTWGKNTDLNSSSIGIELDNNGFKPFT
HHHHHHHCEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCHH
EAQISSLVALLTKLKKDYNIPTQNFLGHADIAPGRKQDPSALFPWKTLAEKGFGIWPDAV
HHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCHHH
LEEAPFDFKIEPALRIIGYNTKNLSAAIQAFKLHYIQTDTTATLDRKTIDTIYSIYKKQI
HHCCCCCEEECCEEEEEECCCHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHC
Q
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]