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The map label for this gene is manA [H]

Identifier: 146302680

GI number: 146302680

Start: 5985582

End: 5986712

Strand: Direct

Name: manA [H]

Synonym: Fjoh_4953

Alternate gene names: 146302680

Gene position: 5985582-5986712 (Clockwise)

Preceding gene: 146302671

Following gene: 146302681

Centisome position: 98.17

GC content: 35.19

Gene sequence:

>1131_bases
ATGAAAAAATACTTTTACACGCTTTTAATCACTGCTTTTATAGGAACTTCCTGTTCTGCCCAGCATAACAATAGTAATAC
GAATTTGTCACTTTCAGATAAAAAAGCAACAGCCGAAACCGTTCTTCTATATAAAAACCTAAACAAACTGGTACAAAAAG
GATATCTTTTTGGTCATCAGGATGACCTTGCTTATGGTGTAAACTGGAAATATGAAAACAACCGAAGCGATATTAAAGAT
GTTGCCGGAGATTATCCCGCAGTTTACGGCTGGGACATTGCTGGTTTAGAAAAAGATGATGCCAATAATATAGACGGCGT
TCCTTTCGAAAAAATGAAGCAATATATTATCGAAGCCAATGAAAGAGGCGGTATTTCAACCATAAGCTGGCATTTTGATA
ATCCCGCAACAGGAAAAAATGCCTGGGACAATACTCCAAATTCACTAAAAACAATTTTACCAGGAGGCGAAAACCATCAA
AAATTTACTTCATGGCTTGATAAAGCCGCCAATTTCTTTCTTTCGTTAAAAGACAAAAAAGGAAAAAACATTCCAATTCT
TTTTAGACCTTATCATGAACTTACCGGAGGCTGGTTTTGGTGGGGAAAAGGAAATTGCACTCCCGAAGAATTTAAAACAG
CATGGAAATTTACTTTTGATTACCTTCAGAAAAAAGGCATTCACAACTTAATTTATGTATACAACACCAGCAGTTTTAAT
ACAAAAGAAGATTTTCTGGCAAATTATCCCGGCGATGATTTTGCCGATATTATAAGCTTTGATTCTTATCAAAACAATAA
TGATAAAGACGGACAAAAATTTATTGAAGAAGTTCAAAAACAATTGAAAATCATCGACGAAATTGGTCATGAAAAACATA
AATTAACAGCCATTGCCGAAGCAGGTTACGAAGCTGTGCCAGATCCAAAATGGTGGACAGGAACTCTCTCTAAAGCCATT
GGCGACTATAAAATTTCGTATGTTCTATTGTGGAGAAACCACGGCTGGCAGGAAAACGAAAAGAAAATGCATTATTATGC
TCCCTTTAAAGGACAAGTAAGCGAAAAAGACTTTATTGAATATTACAATCTCGACAAAACCCTTTTTGAAAAAGACATAA
AAAAGCTTTAA

Upstream 100 bases:

>100_bases
AAACAATACTGAATTACAAATAGACAAAAACAGCTTCAGCAGCTATCTGCAAATTTCCAGCAAATAGTAGAAATACGCTG
AAAAACAATATTTTCACGAA

Downstream 100 bases:

>100_bases
AATAATTACCAGACTCAAAAATCAAACCTTAAAATAACCACTCAATGCACGACAAAATTAGTTTAAAAGAAAAAATTGGT
TACGGACTTGGAGACGCCGC

Product: glycoside hydrolase family protein

Products: NA

Alternate protein names: Mannanase A [H]

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MKKYFYTLLITAFIGTSCSAQHNNSNTNLSLSDKKATAETVLLYKNLNKLVQKGYLFGHQDDLAYGVNWKYENNRSDIKD
VAGDYPAVYGWDIAGLEKDDANNIDGVPFEKMKQYIIEANERGGISTISWHFDNPATGKNAWDNTPNSLKTILPGGENHQ
KFTSWLDKAANFFLSLKDKKGKNIPILFRPYHELTGGWFWWGKGNCTPEEFKTAWKFTFDYLQKKGIHNLIYVYNTSSFN
TKEDFLANYPGDDFADIISFDSYQNNNDKDGQKFIEEVQKQLKIIDEIGHEKHKLTAIAEAGYEAVPDPKWWTGTLSKAI
GDYKISYVLLWRNHGWQENEKKMHYYAPFKGQVSEKDFIEYYNLDKTLFEKDIKKL

Sequences:

>Translated_376_residues
MKKYFYTLLITAFIGTSCSAQHNNSNTNLSLSDKKATAETVLLYKNLNKLVQKGYLFGHQDDLAYGVNWKYENNRSDIKD
VAGDYPAVYGWDIAGLEKDDANNIDGVPFEKMKQYIIEANERGGISTISWHFDNPATGKNAWDNTPNSLKTILPGGENHQ
KFTSWLDKAANFFLSLKDKKGKNIPILFRPYHELTGGWFWWGKGNCTPEEFKTAWKFTFDYLQKKGIHNLIYVYNTSSFN
TKEDFLANYPGDDFADIISFDSYQNNNDKDGQKFIEEVQKQLKIIDEIGHEKHKLTAIAEAGYEAVPDPKWWTGTLSKAI
GDYKISYVLLWRNHGWQENEKKMHYYAPFKGQVSEKDFIEYYNLDKTLFEKDIKKL
>Mature_376_residues
MKKYFYTLLITAFIGTSCSAQHNNSNTNLSLSDKKATAETVLLYKNLNKLVQKGYLFGHQDDLAYGVNWKYENNRSDIKD
VAGDYPAVYGWDIAGLEKDDANNIDGVPFEKMKQYIIEANERGGISTISWHFDNPATGKNAWDNTPNSLKTILPGGENHQ
KFTSWLDKAANFFLSLKDKKGKNIPILFRPYHELTGGWFWWGKGNCTPEEFKTAWKFTFDYLQKKGIHNLIYVYNTSSFN
TKEDFLANYPGDDFADIISFDSYQNNNDKDGQKFIEEVQKQLKIIDEIGHEKHKLTAIAEAGYEAVPDPKWWTGTLSKAI
GDYKISYVLLWRNHGWQENEKKMHYYAPFKGQVSEKDFIEYYNLDKTLFEKDIKKL

Specific function: Hydrolyzes mannan and galactomannan, but displays little activity towards other polysaccharides located in the plant cell wall. Appears to act in synergy with alpha-galactosidase (AgaA) to elicit hydrolysis of galactomannan. Preferentially hydrolyzes the

COG id: COG4124

COG function: function code G; Beta-mannanase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 26 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022790
- InterPro:   IPR000805
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF02156 Glyco_hydro_26 [H]

EC number: =3.2.1.78 [H]

Molecular weight: Translated: 43483; Mature: 43483

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKYFYTLLITAFIGTSCSAQHNNSNTNLSLSDKKATAETVLLYKNLNKLVQKGYLFGHQ
CCHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCC
DDLAYGVNWKYENNRSDIKDVAGDYPAVYGWDIAGLEKDDANNIDGVPFEKMKQYIIEAN
CCCEECCEEEECCCCHHHHHHCCCCCCEECCEECCCCCCCCCCCCCCCHHHHHHHHHHCC
ERGGISTISWHFDNPATGKNAWDNTPNSLKTILPGGENHQKFTSWLDKAANFFLSLKDKK
CCCCCEEEEEECCCCCCCCCCCCCCCCHHHEECCCCCCHHHHHHHHHHHHHEEEEEECCC
GKNIPILFRPYHELTGGWFWWGKGNCTPEEFKTAWKFTFDYLQKKGIHNLIYVYNTSSFN
CCCCCEEEECHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCC
TKEDFLANYPGDDFADIISFDSYQNNNDKDGQKFIEEVQKQLKIIDEIGHEKHKLTAIAE
CHHHHHHCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
AGYEAVPDPKWWTGTLSKAIGDYKISYVLLWRNHGWQENEKKMHYYAPFKGQVSEKDFIE
CCCCCCCCCCCCHHHHHHHHCCEEEEEEEEEECCCCCCCCCCEEEECCCCCCCCCHHHHH
YYNLDKTLFEKDIKKL
HHCCCHHHHHHHHHCC
>Mature Secondary Structure
MKKYFYTLLITAFIGTSCSAQHNNSNTNLSLSDKKATAETVLLYKNLNKLVQKGYLFGHQ
CCHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCC
DDLAYGVNWKYENNRSDIKDVAGDYPAVYGWDIAGLEKDDANNIDGVPFEKMKQYIIEAN
CCCEECCEEEECCCCHHHHHHCCCCCCEECCEECCCCCCCCCCCCCCCHHHHHHHHHHCC
ERGGISTISWHFDNPATGKNAWDNTPNSLKTILPGGENHQKFTSWLDKAANFFLSLKDKK
CCCCCEEEEEECCCCCCCCCCCCCCCCHHHEECCCCCCHHHHHHHHHHHHHEEEEEECCC
GKNIPILFRPYHELTGGWFWWGKGNCTPEEFKTAWKFTFDYLQKKGIHNLIYVYNTSSFN
CCCCCEEEECHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCC
TKEDFLANYPGDDFADIISFDSYQNNNDKDGQKFIEEVQKQLKIIDEIGHEKHKLTAIAE
CHHHHHHCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
AGYEAVPDPKWWTGTLSKAIGDYKISYVLLWRNHGWQENEKKMHYYAPFKGQVSEKDFIE
CCCCCCCCCCCCHHHHHHHHCCEEEEEEEEEECCCCCCCCCCEEEECCCCCCCCCHHHHH
YYNLDKTLFEKDIKKL
HHCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7848261 [H]