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The map label for this gene is rimO

Identifier: 146302669

GI number: 146302669

Start: 5969791

End: 5971104

Strand: Direct

Name: rimO

Synonym: Fjoh_4942

Alternate gene names: 146302669

Gene position: 5969791-5971104 (Clockwise)

Preceding gene: 146302668

Following gene: 146302670

Centisome position: 97.92

GC content: 34.93

Gene sequence:

>1314_bases
ATGAGAACCAAGTCTTTAAAAAAGAACAAAATTAACGTAATCACTCTTGGGTGTTCAAAAAATGTATATGACAGTGAAGT
GCTTATGGGACAACTTCGTGCTAACGGAAAAGAAGTTACACACGAAGCCACTGCTAAGGATGAAGGAAACATTATTGTAA
TTAACACTTGTGGATTTATTGACAACGCGAAAGCTGAATCAGTAAACATGATTTTGGAATATGCTGATAAAAAAGACAAA
GGTTTAGTTGATAAAGTTTTCGTTACCGGATGTTTGTCTGAACGTTACAGACCTGATTTAGAAAAAGAAATTCCAAATGT
GGATCAATATTTTGGTACAACTGAGTTACCTCAATTATTGAAAGCTTTAGGAGCCGATTATAAACATGAATTATTAGGAG
AAAGATTAACTACAACTCCTAAAAATTATGCCTATTTAAAAATTGCCGAAGGATGCGACAGACCTTGCAGTTTCTGTGCA
ATTCCGTTAATGAGAGGTTCGCATGTTTCTCAGCCAATTGAAAAATTAGTTAAAGAAGCACAAGGTTTAGCAAAAAACGG
CGTTAAAGAATTAATATTAATTGCTCAGGATTTGACTTATTACGGTTTAGATCTTTATAAAAAGAGAAATCTGGCAGAAT
TATTAGAAGCTTTAGCAGCTGTTGAAGGAATTGAATGGATTCGTCTGCATTATGCTTATCCTACCGGTTTCCCAATGGAT
GTTTTGGAATTAATGAAACGCGAGCCAAAAATTTGTAATTATATTGATATTCCATTGCAGCATATTTCAGATTCAATTTT
GAAATCAATGCGTCGTGGTACAACTCAGGCTAAAACAACTCAGTTATTAAAAGATTTCCGTGCTGCAGTTCCGGGAATGG
CAATTAGAACTACTTTAATTGTAGGGTATCCAGGAGAAACGCAGGAAGATTTTGAAATTTTGAAAGAGTTTGTTCAGGAA
ATGAAGTTTGACAGAATGGGATGTTTTGCATATTCTCATGAAGAAAACACTCATGCTTACTTACTTGAAGACGATGTTCC
GGACGATGTAAAACAGGCAAGAGCAAATGAAATCATGGAGCTTCAGTCTCAAATTTCATGGGATCTAAACCAAGAGAAAG
TTGGACAAGTATTTAGATGTATCATTGACAGAAAAGAAGGTGCTCATTTTGTGGGAAGAACAGAATTTGACAGCCCGGAT
GTTGACAACGAAGTTTTAATTGACGCATCTAAGCATTATGTAAAAACAGGCGAATTTGTTAATATCAAGATAATTGAAGC
AACAGAATTTGATTTATACGGAGAACCTGCTTAA

Upstream 100 bases:

>100_bases
TGAAAATTACTAGAAACCTTTATAAAAAACATCGAAATGAAACCGAAACAAGCGGTGTTTGAATAAAAAAGTGTAATTTT
GCACTCCAATTTATAAAAGT

Downstream 100 bases:

>100_bases
ATTTACCGTAAACAATTGCTAACGCAGACAAAAATATTTTTATGAAACCTATACAAAACTTAATTATTTTAGTTTTTACT
TTATTCTGTTTCAACTCTGT

Product: MiaB-like tRNA modifying protein YliG

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MRTKSLKKNKINVITLGCSKNVYDSEVLMGQLRANGKEVTHEATAKDEGNIIVINTCGFIDNAKAESVNMILEYADKKDK
GLVDKVFVTGCLSERYRPDLEKEIPNVDQYFGTTELPQLLKALGADYKHELLGERLTTTPKNYAYLKIAEGCDRPCSFCA
IPLMRGSHVSQPIEKLVKEAQGLAKNGVKELILIAQDLTYYGLDLYKKRNLAELLEALAAVEGIEWIRLHYAYPTGFPMD
VLELMKREPKICNYIDIPLQHISDSILKSMRRGTTQAKTTQLLKDFRAAVPGMAIRTTLIVGYPGETQEDFEILKEFVQE
MKFDRMGCFAYSHEENTHAYLLEDDVPDDVKQARANEIMELQSQISWDLNQEKVGQVFRCIIDRKEGAHFVGRTEFDSPD
VDNEVLIDASKHYVKTGEFVNIKIIEATEFDLYGEPA

Sequences:

>Translated_437_residues
MRTKSLKKNKINVITLGCSKNVYDSEVLMGQLRANGKEVTHEATAKDEGNIIVINTCGFIDNAKAESVNMILEYADKKDK
GLVDKVFVTGCLSERYRPDLEKEIPNVDQYFGTTELPQLLKALGADYKHELLGERLTTTPKNYAYLKIAEGCDRPCSFCA
IPLMRGSHVSQPIEKLVKEAQGLAKNGVKELILIAQDLTYYGLDLYKKRNLAELLEALAAVEGIEWIRLHYAYPTGFPMD
VLELMKREPKICNYIDIPLQHISDSILKSMRRGTTQAKTTQLLKDFRAAVPGMAIRTTLIVGYPGETQEDFEILKEFVQE
MKFDRMGCFAYSHEENTHAYLLEDDVPDDVKQARANEIMELQSQISWDLNQEKVGQVFRCIIDRKEGAHFVGRTEFDSPD
VDNEVLIDASKHYVKTGEFVNIKIIEATEFDLYGEPA
>Mature_437_residues
MRTKSLKKNKINVITLGCSKNVYDSEVLMGQLRANGKEVTHEATAKDEGNIIVINTCGFIDNAKAESVNMILEYADKKDK
GLVDKVFVTGCLSERYRPDLEKEIPNVDQYFGTTELPQLLKALGADYKHELLGERLTTTPKNYAYLKIAEGCDRPCSFCA
IPLMRGSHVSQPIEKLVKEAQGLAKNGVKELILIAQDLTYYGLDLYKKRNLAELLEALAAVEGIEWIRLHYAYPTGFPMD
VLELMKREPKICNYIDIPLQHISDSILKSMRRGTTQAKTTQLLKDFRAAVPGMAIRTTLIVGYPGETQEDFEILKEFVQE
MKFDRMGCFAYSHEENTHAYLLEDDVPDDVKQARANEIMELQSQISWDLNQEKVGQVFRCIIDRKEGAHFVGRTEFDSPD
VDNEVLIDASKHYVKTGEFVNIKIIEATEFDLYGEPA

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872782, Length=478, Percent_Identity=25.9414225941423, Blast_Score=165, Evalue=7e-41,
Organism=Homo sapiens, GI28872784, Length=478, Percent_Identity=25.9414225941423, Blast_Score=165, Evalue=8e-41,
Organism=Homo sapiens, GI93277076, Length=449, Percent_Identity=28.0623608017817, Blast_Score=127, Evalue=1e-29,
Organism=Escherichia coli, GI1787057, Length=451, Percent_Identity=39.689578713969, Blast_Score=303, Evalue=1e-83,
Organism=Escherichia coli, GI1786882, Length=447, Percent_Identity=27.7404921700224, Blast_Score=181, Evalue=7e-47,
Organism=Caenorhabditis elegans, GI17553146, Length=469, Percent_Identity=26.0127931769723, Blast_Score=155, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI71996771, Length=302, Percent_Identity=28.8079470198675, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21356207, Length=481, Percent_Identity=25.3638253638254, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI19922432, Length=454, Percent_Identity=24.4493392070485, Blast_Score=114, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_FLAJ1 (A5FA30)

Other databases:

- EMBL:   CP000685
- RefSeq:   YP_001197260.1
- ProteinModelPortal:   A5FA30
- SMR:   A5FA30
- STRING:   A5FA30
- GeneID:   5094212
- GenomeReviews:   CP000685_GR
- KEGG:   fjo:Fjoh_4942
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   KADAPEI
- ProtClustDB:   CLSK2316065
- BioCyc:   FJOH376686:FJOH_4942-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 49497; Mature: 49497

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTKSLKKNKINVITLGCSKNVYDSEVLMGQLRANGKEVTHEATAKDEGNIIVINTCGFI
CCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCHHHHHHCCCCCCCCEEEEEECCCC
DNAKAESVNMILEYADKKDKGLVDKVFVTGCLSERYRPDLEKEIPNVDQYFGTTELPQLL
CCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCHHHHCCCCHHHHHH
KALGADYKHELLGERLTTTPKNYAYLKIAEGCDRPCSFCAIPLMRGSHVSQPIEKLVKEA
HHHCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHH
QGLAKNGVKELILIAQDLTYYGLDLYKKRNLAELLEALAAVEGIEWIRLHYAYPTGFPMD
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCHH
VLELMKREPKICNYIDIPLQHISDSILKSMRRGTTQAKTTQLLKDFRAAVPGMAIRTTLI
HHHHHHCCCCCCCEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEEE
VGYPGETQEDFEILKEFVQEMKFDRMGCFAYSHEENTHAYLLEDDVPDDVKQARANEIME
EECCCCCHHHHHHHHHHHHHHCCCHHCEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHH
LQSQISWDLNQEKVGQVFRCIIDRKEGAHFVGRTEFDSPDVDNEVLIDASKHYVKTGEFV
HHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCHHEEECCCEE
NIKIIEATEFDLYGEPA
EEEEEEEEECCCCCCCC
>Mature Secondary Structure
MRTKSLKKNKINVITLGCSKNVYDSEVLMGQLRANGKEVTHEATAKDEGNIIVINTCGFI
CCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCHHHHHHCCCCCCCCEEEEEECCCC
DNAKAESVNMILEYADKKDKGLVDKVFVTGCLSERYRPDLEKEIPNVDQYFGTTELPQLL
CCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCHHHHCCCCHHHHHH
KALGADYKHELLGERLTTTPKNYAYLKIAEGCDRPCSFCAIPLMRGSHVSQPIEKLVKEA
HHHCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHH
QGLAKNGVKELILIAQDLTYYGLDLYKKRNLAELLEALAAVEGIEWIRLHYAYPTGFPMD
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCHH
VLELMKREPKICNYIDIPLQHISDSILKSMRRGTTQAKTTQLLKDFRAAVPGMAIRTTLI
HHHHHHCCCCCCCEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEEE
VGYPGETQEDFEILKEFVQEMKFDRMGCFAYSHEENTHAYLLEDDVPDDVKQARANEIME
EECCCCCHHHHHHHHHHHHHHCCCHHCEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHH
LQSQISWDLNQEKVGQVFRCIIDRKEGAHFVGRTEFDSPDVDNEVLIDASKHYVKTGEFV
HHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCHHEEECCCEE
NIKIIEATEFDLYGEPA
EEEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA