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The map label for this gene is proS

Identifier: 146302666

GI number: 146302666

Start: 5965457

End: 5966935

Strand: Reverse

Name: proS

Synonym: Fjoh_4939

Alternate gene names: 146302666

Gene position: 5966935-5965457 (Counterclockwise)

Preceding gene: 146302672

Following gene: 146302665

Centisome position: 97.87

GC content: 37.46

Gene sequence:

>1479_bases
ATGAGTAAGAACCTTACTACGCGATCAGAAGATTATTCCAAATGGTATAATGAGCTGGTTGTCAAAGCAGATTTAGCTGA
AAATTCAGGAGTTAGAGGATGTATGGTAATCAAACCATACGGCTATGCAATTTGGGAAAAGATGCAGGCAGAGTTAGATA
GAATGTTTAAAGAAACAGGACATCAAAATGCTTATTTCCCCTTATTTGTGCCGAAGAGTATGTTCGAAGCTGAAGAGAAA
AATGCTGAAGGATTTGCAAAAGAATGTGCTGTTGTAACGCATTACAGACTAAAAAATGACGAGGACAGACCAGGGAAACT
TATGGTTGATCCTAATGCGAAATTAGAAGAAGAGCTTATTGTTCGTCCAACAAGTGAGGCGATTATTTGGTCTACATATA
AAGGATGGGTGCAGTCTTATAGAGATTTGCCTTTACTTATTAATCAATGGGCAAATGTGGTTCGCTGGGAAATGCGTACA
CGTTTGTTTTTAAGAACTGCCGAATTTTTATGGCAGGAAGGGCATACGGCTCACGCTACAAAAGACGAGGCAATTGAGGA
ATCGGAGAAAATGATGAATGTTTATGCTGATTTTGCAGAAAACTTCATGGCAATTCCGGTTGTAAAAGGTTTTAAAACTG
AAACAGAGCGTTTTGCAGGAGCAGATGAAACGTATTGTATTGAAGCTTTGATGCAGGATGGAAAAGCTTTACAGGCTGGA
ACTTCACACTTTTTAGGTCAGAATTTTGCAAAAGCTTTTGATGTGAAATTTGCTAATGCAGAAGGAAAACAAGAGCACGT
TTGGGGAACGTCATGGGGAGTTTCTACTCGTTTGATGGGGGCTTTAATTATGACACATTCTGATGATCAGGGATTGGTGC
TGCCTCCAAATTTAGCTCCAATACAAGTTGTAATTGTACCTATTCATAAAACAGATGAACAATTAGCTCAAATTACAGCG
GCGGTTAATGAATTGACTGCTAAATTGAGAAAGCTTAAGATTTCTGTGAAATATGATGATAGAACAACTCAAAAACCAGG
ATTTAAATTTGCTGAATGGGAATTAAAAGGTGTTCCTGTTAGAATTGCTGTTGGGCCAAAAGATTTAGAAAATGGAACTT
TTGAGGTTGCAAGACGTGATAATTTATCAAAAGAAGTTGTGGCTGCTGAAAAAATAGTGGATCATGTGAATGATTTATTA
GAGCAGATTCAAAAGGATTTATTTGACAAAGCTTTGACTTATAGAAATACTCATATTACAGAAGTAAATAATTTTGAGGA
ATTTAAAGAAGTTTTAGAAGGTAAAGGAGGTTTTATTTCGGCTCATTGGGATGGAACTGCAGCTACTGAAGAAAAGATAA
AAGATTTGACAAAAGCGACCATTCGCTGCATTCCTTTGGATGCTGTTGAAGAGGCGGGAACCTGCGTGTTTACTGGGGAA
CCTTCTTCTAAAAGAGTGCTTTTTGCAAAGGCTTATTAA

Upstream 100 bases:

>100_bases
CTGTGGTGTTTTTTATTGTTGCACATTACAAAAATAGTTAGTTTTGTCAAACTATTTAGTTAAAATTATTTAAAACAAAA
TTTGTGCCAAACTATATAAT

Downstream 100 bases:

>100_bases
AAAAATATAGATTTTTTTGCATTAGGTGTTGCGCAAATAAAAAATAGATGTATCTTTGCATCCGCAATACAGAAGCGCGG
TCCGTTCGTCTATCGGTTAG

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 492; Mature: 491

Protein sequence:

>492_residues
MSKNLTTRSEDYSKWYNELVVKADLAENSGVRGCMVIKPYGYAIWEKMQAELDRMFKETGHQNAYFPLFVPKSMFEAEEK
NAEGFAKECAVVTHYRLKNDEDRPGKLMVDPNAKLEEELIVRPTSEAIIWSTYKGWVQSYRDLPLLINQWANVVRWEMRT
RLFLRTAEFLWQEGHTAHATKDEAIEESEKMMNVYADFAENFMAIPVVKGFKTETERFAGADETYCIEALMQDGKALQAG
TSHFLGQNFAKAFDVKFANAEGKQEHVWGTSWGVSTRLMGALIMTHSDDQGLVLPPNLAPIQVVIVPIHKTDEQLAQITA
AVNELTAKLRKLKISVKYDDRTTQKPGFKFAEWELKGVPVRIAVGPKDLENGTFEVARRDNLSKEVVAAEKIVDHVNDLL
EQIQKDLFDKALTYRNTHITEVNNFEEFKEVLEGKGGFISAHWDGTAATEEKIKDLTKATIRCIPLDAVEEAGTCVFTGE
PSSKRVLFAKAY

Sequences:

>Translated_492_residues
MSKNLTTRSEDYSKWYNELVVKADLAENSGVRGCMVIKPYGYAIWEKMQAELDRMFKETGHQNAYFPLFVPKSMFEAEEK
NAEGFAKECAVVTHYRLKNDEDRPGKLMVDPNAKLEEELIVRPTSEAIIWSTYKGWVQSYRDLPLLINQWANVVRWEMRT
RLFLRTAEFLWQEGHTAHATKDEAIEESEKMMNVYADFAENFMAIPVVKGFKTETERFAGADETYCIEALMQDGKALQAG
TSHFLGQNFAKAFDVKFANAEGKQEHVWGTSWGVSTRLMGALIMTHSDDQGLVLPPNLAPIQVVIVPIHKTDEQLAQITA
AVNELTAKLRKLKISVKYDDRTTQKPGFKFAEWELKGVPVRIAVGPKDLENGTFEVARRDNLSKEVVAAEKIVDHVNDLL
EQIQKDLFDKALTYRNTHITEVNNFEEFKEVLEGKGGFISAHWDGTAATEEKIKDLTKATIRCIPLDAVEEAGTCVFTGE
PSSKRVLFAKAY
>Mature_491_residues
SKNLTTRSEDYSKWYNELVVKADLAENSGVRGCMVIKPYGYAIWEKMQAELDRMFKETGHQNAYFPLFVPKSMFEAEEKN
AEGFAKECAVVTHYRLKNDEDRPGKLMVDPNAKLEEELIVRPTSEAIIWSTYKGWVQSYRDLPLLINQWANVVRWEMRTR
LFLRTAEFLWQEGHTAHATKDEAIEESEKMMNVYADFAENFMAIPVVKGFKTETERFAGADETYCIEALMQDGKALQAGT
SHFLGQNFAKAFDVKFANAEGKQEHVWGTSWGVSTRLMGALIMTHSDDQGLVLPPNLAPIQVVIVPIHKTDEQLAQITAA
VNELTAKLRKLKISVKYDDRTTQKPGFKFAEWELKGVPVRIAVGPKDLENGTFEVARRDNLSKEVVAAEKIVDHVNDLLE
QIQKDLFDKALTYRNTHITEVNNFEEFKEVLEGKGGFISAHWDGTAATEEKIKDLTKATIRCIPLDAVEEAGTCVFTGEP
SSKRVLFAKAY

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro)

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily

Homologues:

Organism=Homo sapiens, GI62241042, Length=509, Percent_Identity=39.0962671905697, Blast_Score=369, Evalue=1e-102,
Organism=Escherichia coli, GI1786392, Length=173, Percent_Identity=29.4797687861272, Blast_Score=79, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI71991342, Length=505, Percent_Identity=38.8118811881188, Blast_Score=367, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI71991346, Length=368, Percent_Identity=43.75, Blast_Score=327, Evalue=6e-90,
Organism=Saccharomyces cerevisiae, GI6321808, Length=511, Percent_Identity=38.747553816047, Blast_Score=362, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24649468, Length=455, Percent_Identity=40, Blast_Score=345, Evalue=3e-95,
Organism=Drosophila melanogaster, GI24649466, Length=455, Percent_Identity=40, Blast_Score=345, Evalue=6e-95,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_FLAJ1 (A5FA31)

Other databases:

- EMBL:   CP000685
- RefSeq:   YP_001197257.1
- ProteinModelPortal:   A5FA31
- SMR:   A5FA31
- STRING:   A5FA31
- GeneID:   5094136
- GenomeReviews:   CP000685_GR
- KEGG:   fjo:Fjoh_4939
- eggNOG:   COG0442
- HOGENOM:   HBG334108
- OMA:   PGWKFNE
- ProtClustDB:   PRK08661
- BioCyc:   FJOH376686:FJOH_4939-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01571
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR004499
- InterPro:   IPR017449
- InterPro:   IPR016061
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.30.110.30
- PANTHER:   PTHR11451:SF6
- SMART:   SM00946
- TIGRFAMs:   TIGR00408

Pfam domain/function: PF03129 HGTP_anticodon; PF09180 ProRS-C_1; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd; SSF64586 Pro-tRNA_synth_II_C

EC number: =6.1.1.15

Molecular weight: Translated: 55784; Mature: 55653

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKNLTTRSEDYSKWYNELVVKADLAENSGVRGCMVIKPYGYAIWEKMQAELDRMFKETG
CCCCCCCCCHHHHHHHHHHEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCC
HQNAYFPLFVPKSMFEAEEKNAEGFAKECAVVTHYRLKNDEDRPGKLMVDPNAKLEEELI
CCCCEEEEECCHHHHHHHHCCCCHHHHHHHHHEEEEECCCCCCCCEEEECCCCCCCHHHE
VRPTSEAIIWSTYKGWVQSYRDLPLLINQWANVVRWEMRTRLFLRTAEFLWQEGHTAHAT
ECCCCCEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
KDEAIEESEKMMNVYADFAENFMAIPVVKGFKTETERFAGADETYCIEALMQDGKALQAG
HHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHCCCCHHHHHHHHHHCCCHHHHH
TSHFLGQNFAKAFDVKFANAEGKQEHVWGTSWGVSTRLMGALIMTHSDDQGLVLPPNLAP
HHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCHHHHHHHHHEEEECCCCCEEECCCCCC
IQVVIVPIHKTDEQLAQITAAVNELTAKLRKLKISVKYDDRTTQKPGFKFAEWELKGVPV
EEEEEEECCCCHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCEEEEEEECCCEE
RIAVGPKDLENGTFEVARRDNLSKEVVAAEKIVDHVNDLLEQIQKDLFDKALTYRNTHIT
EEEECCCCCCCCCEEEHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
EVNNFEEFKEVLEGKGGFISAHWDGTAATEEKIKDLTKATIRCIPLDAVEEAGTCVFTGE
ECCCHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHEEEECCHHHHHCCCEEEECC
PSSKRVLFAKAY
CCCCEEEEEECC
>Mature Secondary Structure 
SKNLTTRSEDYSKWYNELVVKADLAENSGVRGCMVIKPYGYAIWEKMQAELDRMFKETG
CCCCCCCCHHHHHHHHHHEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCC
HQNAYFPLFVPKSMFEAEEKNAEGFAKECAVVTHYRLKNDEDRPGKLMVDPNAKLEEELI
CCCCEEEEECCHHHHHHHHCCCCHHHHHHHHHEEEEECCCCCCCCEEEECCCCCCCHHHE
VRPTSEAIIWSTYKGWVQSYRDLPLLINQWANVVRWEMRTRLFLRTAEFLWQEGHTAHAT
ECCCCCEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
KDEAIEESEKMMNVYADFAENFMAIPVVKGFKTETERFAGADETYCIEALMQDGKALQAG
HHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHCCCCHHHHHHHHHHCCCHHHHH
TSHFLGQNFAKAFDVKFANAEGKQEHVWGTSWGVSTRLMGALIMTHSDDQGLVLPPNLAP
HHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCHHHHHHHHHEEEECCCCCEEECCCCCC
IQVVIVPIHKTDEQLAQITAAVNELTAKLRKLKISVKYDDRTTQKPGFKFAEWELKGVPV
EEEEEEECCCCHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCEEEEEEECCCEE
RIAVGPKDLENGTFEVARRDNLSKEVVAAEKIVDHVNDLLEQIQKDLFDKALTYRNTHIT
EEEECCCCCCCCCEEEHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
EVNNFEEFKEVLEGKGGFISAHWDGTAATEEKIKDLTKATIRCIPLDAVEEAGTCVFTGE
ECCCHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHEEEECCHHHHHCCCEEEECC
PSSKRVLFAKAY
CCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA