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The map label for this gene is btuB [H]

Identifier: 146302548

GI number: 146302548

Start: 5837252

End: 5839117

Strand: Reverse

Name: btuB [H]

Synonym: Fjoh_4821

Alternate gene names: 146302548

Gene position: 5839117-5837252 (Counterclockwise)

Preceding gene: 146302554

Following gene: 146302540

Centisome position: 95.77

GC content: 34.14

Gene sequence:

>1866_bases
ATGAAAAAAATCTATTTTGCAGTCTTTACATTAGTTTGCACAAATTTTTATGCCCAAACCAAAAGGGATTCTATAATAGA
AATTGATGAAGTGATTATTAACGAAAACCGTTTAAGCACACCAATTTCTAAACAAAACAGAAACGTATACGTTATAAACA
GCGAAACAATAAAAAAATTACCGGGCCGTACTTTACAGGAAGTATTGCAATATGCTAACGGAGTTGATATTAGACAAAGA
GGTCCTTTTGGCGGTCAGGCCGATGTAAGTGTCGACGGCGGAAGCTTTGAACAAACTGTAGTTTTATTAAACGGAGCCAA
AATTATTGATTCGCAAACGGCTCATAATATGTTAAATCTGCCTCTTCCGGTAGAAGTTATTGAAAGAATTGAAGTTTTGC
GTGGTCCGGCTGCCAGAATTTTTGGAATTAACAGTTTAACGGGAGCCATTAATATTATTACCAAAAAACCAACCGATTCA
GGTTTTATGGTAAGTACTTATGCAGGTTCTAATTTTGAAAAAGATACAGAAAACACAGGCGATACTTTTTACGGAACGGG
CATTCAGGCAGGAGCTGTTTTAGGAAAAGAAAAACAACAGCATTCGATTTTTGCATCGCATGATAAAAGCAACGGATACC
GTTACAATACGGGTTTTGAAAACAATAAATTATTGTATCAGGGAAATGTTCAAATCAATGATTCTAACGAAATTTTAGGT
TCATTTGGTTATGTTAAAAACGGATTTGGTGCCAACGGATTTTATGCTGCACCGGGCGACAGAAATTCTACTGAAATAGT
ACAGACTACTTTTGCCAACATTCAGTCGAAACATGCTATTACAGACAGATGGAAAATTATGCCGAGAGTTACCTACAGAT
ATAATTATGACGATTACAGGTATTTAGGAAACGGAAATTTAAATGCAGGAAGAAGCCAGCATTATACGAATTCTATTGCA
GCAGAATTAAACTCGTCTATTAAAACAGAAAATGGCGAAATTGGTTTTGGTGCCGAATTTAGAAATGAGGATATTCATTC
GTCTAATATTGGAGATCATGATCGCGAAAATGTTGGAATTTATGCCGAATACAGAACGTATTTGTTTGAAAAATTAAATG
TGAATGTAGGAACGTATTTAAATTACAATTCAGATTATAAATGGCAGATTTATCCTGGAATTGATGCGAGTTATGAATTA
ACGGAGGAATTTAAAATTATTGGAAACATCGGCACAAGCCAAAGAATTCCGTCTTTTACAGATTTATATCTAAACCAGCG
TCCGGGAAATATTGGAAATCCTGATCTTGATTCTGAAAATGCTTTTCAAAGTGAAGTAGGGTTTAAATTCAACAGAAAGA
ATTTGAATTTAAATGTGAATTATTTCTACAGAGAAATCAGCAATTATATCGATTGGACCCGTATGACTACAGCAGTGCCG
TGGCAGAGTCAAAACTACGGGGATTTGATTACAAACGGAATCAATATGCGCGGCAGTTACAGAATTGATTTTTCTGCAGA
TTCTAAATTAAATTTCACTTTAGGTTATTCGTATTTAGATTCTAAATTTAAAGACACTCGTTCTGAGCCATTTTCTAAAT
ATTTGATTTCTTCTTTGAAACATCAAATTACTAACACAATTGATTTCCAGCATAAAGATTTTTCAGTTTTGTTTGCCACT
CGTTTTAATGAAAGAGTAACGGGAGCTTCTTACTGGATTAATGATTTTAGAGTAAGTCAGTCAGTTAAAAAATTTACATT
TTTCGTAGATGCTCAAAACATTTTTAATACAACTTATTTTGAAGTTGGAGCATTTCCGCTGCCGTCAAGATGGTTTAGTG
CAGGTGTAAAATTTGTGAGTTTTTAA

Upstream 100 bases:

>100_bases
AGCTTTTAAAAGGAATTTGAACCGGACCACTCATAATTACAAAACTGGTATACTTTATTAGTACGGCAAGATACTACTTT
TGCAAAAATTTTAAACCAAA

Downstream 100 bases:

>100_bases
ATTATAAAGGTTAGTTATATAAAAAGGGAAGACGTTTAATGTCTTCCCTTTTTTGTTTACCATAATTTTTATTGTGAGAA
GAAACGAAATATTCTCGGTT

Product: TonB-dependent receptor

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 621; Mature: 621

Protein sequence:

>621_residues
MKKIYFAVFTLVCTNFYAQTKRDSIIEIDEVIINENRLSTPISKQNRNVYVINSETIKKLPGRTLQEVLQYANGVDIRQR
GPFGGQADVSVDGGSFEQTVVLLNGAKIIDSQTAHNMLNLPLPVEVIERIEVLRGPAARIFGINSLTGAINIITKKPTDS
GFMVSTYAGSNFEKDTENTGDTFYGTGIQAGAVLGKEKQQHSIFASHDKSNGYRYNTGFENNKLLYQGNVQINDSNEILG
SFGYVKNGFGANGFYAAPGDRNSTEIVQTTFANIQSKHAITDRWKIMPRVTYRYNYDDYRYLGNGNLNAGRSQHYTNSIA
AELNSSIKTENGEIGFGAEFRNEDIHSSNIGDHDRENVGIYAEYRTYLFEKLNVNVGTYLNYNSDYKWQIYPGIDASYEL
TEEFKIIGNIGTSQRIPSFTDLYLNQRPGNIGNPDLDSENAFQSEVGFKFNRKNLNLNVNYFYREISNYIDWTRMTTAVP
WQSQNYGDLITNGINMRGSYRIDFSADSKLNFTLGYSYLDSKFKDTRSEPFSKYLISSLKHQITNTIDFQHKDFSVLFAT
RFNERVTGASYWINDFRVSQSVKKFTFFVDAQNIFNTTYFEVGAFPLPSRWFSAGVKFVSF

Sequences:

>Translated_621_residues
MKKIYFAVFTLVCTNFYAQTKRDSIIEIDEVIINENRLSTPISKQNRNVYVINSETIKKLPGRTLQEVLQYANGVDIRQR
GPFGGQADVSVDGGSFEQTVVLLNGAKIIDSQTAHNMLNLPLPVEVIERIEVLRGPAARIFGINSLTGAINIITKKPTDS
GFMVSTYAGSNFEKDTENTGDTFYGTGIQAGAVLGKEKQQHSIFASHDKSNGYRYNTGFENNKLLYQGNVQINDSNEILG
SFGYVKNGFGANGFYAAPGDRNSTEIVQTTFANIQSKHAITDRWKIMPRVTYRYNYDDYRYLGNGNLNAGRSQHYTNSIA
AELNSSIKTENGEIGFGAEFRNEDIHSSNIGDHDRENVGIYAEYRTYLFEKLNVNVGTYLNYNSDYKWQIYPGIDASYEL
TEEFKIIGNIGTSQRIPSFTDLYLNQRPGNIGNPDLDSENAFQSEVGFKFNRKNLNLNVNYFYREISNYIDWTRMTTAVP
WQSQNYGDLITNGINMRGSYRIDFSADSKLNFTLGYSYLDSKFKDTRSEPFSKYLISSLKHQITNTIDFQHKDFSVLFAT
RFNERVTGASYWINDFRVSQSVKKFTFFVDAQNIFNTTYFEVGAFPLPSRWFSAGVKFVSF
>Mature_621_residues
MKKIYFAVFTLVCTNFYAQTKRDSIIEIDEVIINENRLSTPISKQNRNVYVINSETIKKLPGRTLQEVLQYANGVDIRQR
GPFGGQADVSVDGGSFEQTVVLLNGAKIIDSQTAHNMLNLPLPVEVIERIEVLRGPAARIFGINSLTGAINIITKKPTDS
GFMVSTYAGSNFEKDTENTGDTFYGTGIQAGAVLGKEKQQHSIFASHDKSNGYRYNTGFENNKLLYQGNVQINDSNEILG
SFGYVKNGFGANGFYAAPGDRNSTEIVQTTFANIQSKHAITDRWKIMPRVTYRYNYDDYRYLGNGNLNAGRSQHYTNSIA
AELNSSIKTENGEIGFGAEFRNEDIHSSNIGDHDRENVGIYAEYRTYLFEKLNVNVGTYLNYNSDYKWQIYPGIDASYEL
TEEFKIIGNIGTSQRIPSFTDLYLNQRPGNIGNPDLDSENAFQSEVGFKFNRKNLNLNVNYFYREISNYIDWTRMTTAVP
WQSQNYGDLITNGINMRGSYRIDFSADSKLNFTLGYSYLDSKFKDTRSEPFSKYLISSLKHQITNTIDFQHKDFSVLFAT
RFNERVTGASYWINDFRVSQSVKKFTFFVDAQNIFNTTYFEVGAFPLPSRWFSAGVKFVSF

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4771

COG function: function code P; Outer membrane receptor for ferrienterochelin and colicins

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=634, Percent_Identity=22.397476340694, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1788478, Length=471, Percent_Identity=22.5053078556263, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 70502; Mature: 70502

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIYFAVFTLVCTNFYAQTKRDSIIEIDEVIINENRLSTPISKQNRNVYVINSETIKKL
CCCHHHHHHHHHHHHHHHHCCCCCCEEEEHEEECCCCCCCCCCCCCCEEEEECCHHHHHC
PGRTLQEVLQYANGVDIRQRGPFGGQADVSVDGGSFEQTVVLLNGAKIIDSQTAHNMLNL
CCHHHHHHHHHHCCCCEEECCCCCCCEEEEECCCCCCEEEEEEECCEEECCHHHHCEECC
PLPVEVIERIEVLRGPAARIFGINSLTGAINIITKKPTDSGFMVSTYAGSNFEKDTENTG
CCCHHHHHHHHHHCCCCCEEEECCCCCCEEEEEEECCCCCCEEEEEECCCCCCCCCCCCC
DTFYGTGIQAGAVLGKEKQQHSIFASHDKSNGYRYNTGFENNKLLYQGNVQINDSNEILG
CEEEECCCCCCHHHCCCCHHCCEEEECCCCCCEEEECCCCCCEEEEECCEEECCCHHHHH
SFGYVKNGFGANGFYAAPGDRNSTEIVQTTFANIQSKHAITDRWKIMPRVTYRYNYDDYR
CCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEECCCCEE
YLGNGNLNAGRSQHYTNSIAAELNSSIKTENGEIGFGAEFRNEDIHSSNIGDHDRENVGI
EECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCE
YAEYRTYLFEKLNVNVGTYLNYNSDYKWQIYPGIDASYELTEEFKIIGNIGTSQRIPSFT
EEHHHHHHHHHHCCCEEEEEECCCCEEEEEECCCCCCEECCEEEEEEECCCCCCCCCCHH
DLYLNQRPGNIGNPDLDSENAFQSEVGFKFNRKNLNLNVNYFYREISNYIDWTRMTTAVP
HHEEECCCCCCCCCCCCCCCCHHHHCCCEEECCEEEEEEHHHHHHHHHHHHHHHHEEECC
WQSQNYGDLITNGINMRGSYRIDFSADSKLNFTLGYSYLDSKFKDTRSEPFSKYLISSLK
CCCCCCCHHHHCCCCCCEEEEEEECCCCEEEEEEEHHHHHHHHHHCCCCHHHHHHHHHHH
HQITNTIDFQHKDFSVLFATRFNERVTGASYWINDFRVSQSVKKFTFFVDAQNIFNTTYF
HHHHHCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCEEEEEEEECHHHHCCEEE
EVGAFPLPSRWFSAGVKFVSF
EECCCCCCHHHHHCCEEEECC
>Mature Secondary Structure
MKKIYFAVFTLVCTNFYAQTKRDSIIEIDEVIINENRLSTPISKQNRNVYVINSETIKKL
CCCHHHHHHHHHHHHHHHHCCCCCCEEEEHEEECCCCCCCCCCCCCCEEEEECCHHHHHC
PGRTLQEVLQYANGVDIRQRGPFGGQADVSVDGGSFEQTVVLLNGAKIIDSQTAHNMLNL
CCHHHHHHHHHHCCCCEEECCCCCCCEEEEECCCCCCEEEEEEECCEEECCHHHHCEECC
PLPVEVIERIEVLRGPAARIFGINSLTGAINIITKKPTDSGFMVSTYAGSNFEKDTENTG
CCCHHHHHHHHHHCCCCCEEEECCCCCCEEEEEEECCCCCCEEEEEECCCCCCCCCCCCC
DTFYGTGIQAGAVLGKEKQQHSIFASHDKSNGYRYNTGFENNKLLYQGNVQINDSNEILG
CEEEECCCCCCHHHCCCCHHCCEEEECCCCCCEEEECCCCCCEEEEECCEEECCCHHHHH
SFGYVKNGFGANGFYAAPGDRNSTEIVQTTFANIQSKHAITDRWKIMPRVTYRYNYDDYR
CCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEECCCCEE
YLGNGNLNAGRSQHYTNSIAAELNSSIKTENGEIGFGAEFRNEDIHSSNIGDHDRENVGI
EECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCE
YAEYRTYLFEKLNVNVGTYLNYNSDYKWQIYPGIDASYELTEEFKIIGNIGTSQRIPSFT
EEHHHHHHHHHHCCCEEEEEECCCCEEEEEECCCCCCEECCEEEEEEECCCCCCCCCCHH
DLYLNQRPGNIGNPDLDSENAFQSEVGFKFNRKNLNLNVNYFYREISNYIDWTRMTTAVP
HHEEECCCCCCCCCCCCCCCCHHHHCCCEEECCEEEEEEHHHHHHHHHHHHHHHHEEECC
WQSQNYGDLITNGINMRGSYRIDFSADSKLNFTLGYSYLDSKFKDTRSEPFSKYLISSLK
CCCCCCCHHHHCCCCCCEEEEEEECCCCEEEEEEEHHHHHHHHHHCCCCHHHHHHHHHHH
HQITNTIDFQHKDFSVLFATRFNERVTGASYWINDFRVSQSVKKFTFFVDAQNIFNTTYF
HHHHHCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCEEEEEEEECHHHHCCEEE
EVGAFPLPSRWFSAGVKFVSF
EECCCCCCHHHHHCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA