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The map label for this gene is nagB [C]

Identifier: 146302539

GI number: 146302539

Start: 5820361

End: 5822289

Strand: Reverse

Name: nagB [C]

Synonym: Fjoh_4812

Alternate gene names: 146302539

Gene position: 5822289-5820361 (Counterclockwise)

Preceding gene: 146302540

Following gene: 146302538

Centisome position: 95.5

GC content: 36.5

Gene sequence:

>1929_bases
ATGAAAACCACTACTTCTTTGGCTTACGATATTCCGGGAAAATTTGAAGAAACACGATTTGAAAAAAATCATAATGTAAT
CTTTGAAAACTCTGCCGAAGCTTCAAAAAATGTCGCTCAAGAAATAGCTGAATTAATACGTTTAAAACAAACTAACAACG
AATCATGTGTACTTGGCCTTGCAACAGGCTCTTCACCAATTAAAGTATACGAAGAACTGGTTCGAATGCATAAAGAAGAA
GGTCTTAGCTTTAGTAACGTAATTACTTTTAATTTGGATGAATATTATCCAATGAATAAAGAAAACCGTCAAAGCTATCA
TTATTTCATGCATCAGCATCTTTTTAATCATATTGATATTAAACCTGAAAATGTAAATATTCCCGACGGAACAATTGCTT
TAGAACAATTAAATCAATACTGCATTGATTATGAATTGAAAATTAAACAAGCAGGCGGACTTGATTTTCAATTGCTTGGA
ATTGGGCGTACAGGACACGTTGGTTTCAATGAGCCTGGATCACACATTAATTCAGGAACCAGAATTATTACTCTGGATCA
TATTACAAGAGTAGATGCTTCATCTGACTTTAACGGAATTGACAACGTTCCAAAAAGAGCCATCACGATGGGAGTTTCTA
CCATTATGAGATCAAAAAGAATTGTATTAATGGCGTGGGGACAAAACAAAGCGTCTATTATAAAGAGAACCATTCAGGGC
GAAATAAGTTCTGATGTTCCTGCAACGTTTTTACAAAATCATCCAAACGCTACTTTCGTTTTAGACCAATCAGCAGCATC
AGAATTGACACGCTTTAAAACACCTTGGCTGGTTGGAGAATGTATCTGGACAGAAGAATTAAAGAGCAAAGCAATCGTTT
GGCTTTGTAAAAAAACAAAACAATCTATTTTAAAACTGACAGATCGTGATTACAACAATAACGGAATGTCTGATTTATTA
GCACATGGCAGTTCTGCCTACGATTTGAATATAAATATGTTCAATATTTTGCAGCATACTATTACAGGCTGGCCGGGAGG
AAAACCAAACACAGATGATTCACACCGTCCGGAAAGAGCCAATCCTGCCAAAAAACGAGTAATCCTGTTTAGTCCGCATC
CAGATGATGACGTGATTTCGATGGGAGGAACTTTTTCAAAATTGATAAAACAAGGCCACGATGTACATGTTGTGTATCAA
ACCTCCGGAAATATTGCCGTTACAGATGATGAGGCATTGAAATTTGCAGAAGTATGCAACGATTTTGTTGGTGCAGATCT
TCCAAAAGACATTAACTTCAAATCAGTGATTGAATTTTTGAATAACAAATCAGAAAATCAGATTGATTCTCTTGAAGTTC
GAAAACTAAAAGGATTAATCAGAAGAAGAGAGTCTTACGCAGCAACAAGATACATTGGACTAAAAGATGAAAATACACAC
TTTTTGGATCTTCCGTTTTATGAAACCGGACAGGTTAAAAAGAATCCGCTGGGTCAGGAAGACATCGAAATTGTAAAAGA
AATCATTGCCAGAATAAAACCGCATCAGGTTTTTGCAGCAGGAGATTTGGCTGATCCGCATGGAACACATGAAGTATGTC
TGAATGCGATTTTTGCAGCCATGAAAGAATTGAAACCAGAAAAATACATGGACGACTGCTGGTTATGGTTATACAGAGGT
GCATGGCACGAATGGGATATCCACGAAATTGATATGGCAGTGCCGCTTTCTCCATCTGAAGTATTGTTGAAACGCCACGC
CATTTTGTATCATCAATCTCAAAAAGACAGAGTAATGTTCCAGGGAAATGATTCTAGAGAATTCTGGGTTAGAGCCGAAG
ACCGAAATAAAAATACTGCAATTCTGTATGATGAATTAGGATTGGCAGAATATGAAGCTATTGAAGCTTTTAAACGTTTT
GATTATTAG

Upstream 100 bases:

>100_bases
TATTTGAATTTAATTTTGTTAAATAACGCAAAATAATGTATTTGTTATCTTTTTTGTATATATATTTGTTAAAACTAATT
TTAACTACAACAGATATATT

Downstream 100 bases:

>100_bases
TAATTAATAAAAAAAATCATTTAATACATAGAAACATAGATTTTATTGTGTGTAAAAGAATATAAAAAGAAACATGTTTC
TAACACATAGTTCTCTATGC

Product: glucosamine-6-phosphate deaminase-like protein

Products: D-fructose 6-phosphate; NH3

Alternate protein names: NA

Number of amino acids: Translated: 642; Mature: 642

Protein sequence:

>642_residues
MKTTTSLAYDIPGKFEETRFEKNHNVIFENSAEASKNVAQEIAELIRLKQTNNESCVLGLATGSSPIKVYEELVRMHKEE
GLSFSNVITFNLDEYYPMNKENRQSYHYFMHQHLFNHIDIKPENVNIPDGTIALEQLNQYCIDYELKIKQAGGLDFQLLG
IGRTGHVGFNEPGSHINSGTRIITLDHITRVDASSDFNGIDNVPKRAITMGVSTIMRSKRIVLMAWGQNKASIIKRTIQG
EISSDVPATFLQNHPNATFVLDQSAASELTRFKTPWLVGECIWTEELKSKAIVWLCKKTKQSILKLTDRDYNNNGMSDLL
AHGSSAYDLNINMFNILQHTITGWPGGKPNTDDSHRPERANPAKKRVILFSPHPDDDVISMGGTFSKLIKQGHDVHVVYQ
TSGNIAVTDDEALKFAEVCNDFVGADLPKDINFKSVIEFLNNKSENQIDSLEVRKLKGLIRRRESYAATRYIGLKDENTH
FLDLPFYETGQVKKNPLGQEDIEIVKEIIARIKPHQVFAAGDLADPHGTHEVCLNAIFAAMKELKPEKYMDDCWLWLYRG
AWHEWDIHEIDMAVPLSPSEVLLKRHAILYHQSQKDRVMFQGNDSREFWVRAEDRNKNTAILYDELGLAEYEAIEAFKRF
DY

Sequences:

>Translated_642_residues
MKTTTSLAYDIPGKFEETRFEKNHNVIFENSAEASKNVAQEIAELIRLKQTNNESCVLGLATGSSPIKVYEELVRMHKEE
GLSFSNVITFNLDEYYPMNKENRQSYHYFMHQHLFNHIDIKPENVNIPDGTIALEQLNQYCIDYELKIKQAGGLDFQLLG
IGRTGHVGFNEPGSHINSGTRIITLDHITRVDASSDFNGIDNVPKRAITMGVSTIMRSKRIVLMAWGQNKASIIKRTIQG
EISSDVPATFLQNHPNATFVLDQSAASELTRFKTPWLVGECIWTEELKSKAIVWLCKKTKQSILKLTDRDYNNNGMSDLL
AHGSSAYDLNINMFNILQHTITGWPGGKPNTDDSHRPERANPAKKRVILFSPHPDDDVISMGGTFSKLIKQGHDVHVVYQ
TSGNIAVTDDEALKFAEVCNDFVGADLPKDINFKSVIEFLNNKSENQIDSLEVRKLKGLIRRRESYAATRYIGLKDENTH
FLDLPFYETGQVKKNPLGQEDIEIVKEIIARIKPHQVFAAGDLADPHGTHEVCLNAIFAAMKELKPEKYMDDCWLWLYRG
AWHEWDIHEIDMAVPLSPSEVLLKRHAILYHQSQKDRVMFQGNDSREFWVRAEDRNKNTAILYDELGLAEYEAIEAFKRF
DY
>Mature_642_residues
MKTTTSLAYDIPGKFEETRFEKNHNVIFENSAEASKNVAQEIAELIRLKQTNNESCVLGLATGSSPIKVYEELVRMHKEE
GLSFSNVITFNLDEYYPMNKENRQSYHYFMHQHLFNHIDIKPENVNIPDGTIALEQLNQYCIDYELKIKQAGGLDFQLLG
IGRTGHVGFNEPGSHINSGTRIITLDHITRVDASSDFNGIDNVPKRAITMGVSTIMRSKRIVLMAWGQNKASIIKRTIQG
EISSDVPATFLQNHPNATFVLDQSAASELTRFKTPWLVGECIWTEELKSKAIVWLCKKTKQSILKLTDRDYNNNGMSDLL
AHGSSAYDLNINMFNILQHTITGWPGGKPNTDDSHRPERANPAKKRVILFSPHPDDDVISMGGTFSKLIKQGHDVHVVYQ
TSGNIAVTDDEALKFAEVCNDFVGADLPKDINFKSVIEFLNNKSENQIDSLEVRKLKGLIRRRESYAATRYIGLKDENTH
FLDLPFYETGQVKKNPLGQEDIEIVKEIIARIKPHQVFAAGDLADPHGTHEVCLNAIFAAMKELKPEKYMDDCWLWLYRG
AWHEWDIHEIDMAVPLSPSEVLLKRHAILYHQSQKDRVMFQGNDSREFWVRAEDRNKNTAILYDELGLAEYEAIEAFKRF
DY

Specific function: Catalyzes The Reversible Isomerization-Deamination Of Glucosamine 6-Phosphate (Glcn6p) To Form Fructose 6-Phosphate (Fru6p) And Ammonium Ion. [C]

COG id: COG0363

COG function: function code G; 6-phosphogluconolactonase/Glucosamine-6-phosphate isomerase/deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the glucosamine/galactosamine-6-phosphate isomerase family. NagB subfamily [H]

Homologues:

Organism=Homo sapiens, GI19923881, Length=251, Percent_Identity=39.0438247011952, Blast_Score=169, Evalue=1e-41,
Organism=Homo sapiens, GI13027378, Length=246, Percent_Identity=37.3983739837398, Blast_Score=165, Evalue=2e-40,
Organism=Escherichia coli, GI1786893, Length=223, Percent_Identity=39.4618834080717, Blast_Score=173, Evalue=4e-44,
Organism=Escherichia coli, GI1789530, Length=229, Percent_Identity=26.2008733624454, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI48994958, Length=241, Percent_Identity=26.5560165975104, Blast_Score=78, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17554876, Length=246, Percent_Identity=38.2113821138211, Blast_Score=173, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24581960, Length=252, Percent_Identity=39.2857142857143, Blast_Score=175, Evalue=8e-44,
Organism=Drosophila melanogaster, GI19920764, Length=252, Percent_Identity=39.2857142857143, Blast_Score=175, Evalue=8e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006148
- InterPro:   IPR003737
- InterPro:   IPR004547
- InterPro:   IPR018321 [H]

Pfam domain/function: PF01182 Glucosamine_iso; PF02585 PIG-L [H]

EC number: 3.5.99.6

Molecular weight: Translated: 73152; Mature: 73152

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTTTSLAYDIPGKFEETRFEKNHNVIFENSAEASKNVAQEIAELIRLKQTNNESCVLGL
CCCCCCEEECCCCCHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCEEEEE
ATGSSPIKVYEELVRMHKEEGLSFSNVITFNLDEYYPMNKENRQSYHYFMHQHLFNHIDI
ECCCCHHHHHHHHHHHHHHHCCCCHHEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCC
KPENVNIPDGTIALEQLNQYCIDYELKIKQAGGLDFQLLGIGRTGHVGFNEPGSHINSGT
CCCCCCCCCCHHHHHHHHHHHCCEEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCC
RIITLDHITRVDASSDFNGIDNVPKRAITMGVSTIMRSKRIVLMAWGQNKASIIKRTIQG
EEEEEECEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHC
EISSDVPATFLQNHPNATFVLDQSAASELTRFKTPWLVGECIWTEELKSKAIVWLCKKTK
CCCCCCCHHHHHCCCCEEEEECCHHHHHHHHCCCCCEEEHEEEHHHHHCCEEEEEEHHHH
QSILKLTDRDYNNNGMSDLLAHGSSAYDLNINMFNILQHTITGWPGGKPNTDDSHRPERA
HHHHHHHCCCCCCCCHHHHHHCCCCEEEEECHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
NPAKKRVILFSPHPDDDVISMGGTFSKLIKQGHDVHVVYQTSGNIAVTDDEALKFAEVCN
CCCCCEEEEECCCCCCCCEECCCHHHHHHHCCCCEEEEEEECCCEEECCHHHHHHHHHHH
DFVGADLPKDINFKSVIEFLNNKSENQIDSLEVRKLKGLIRRRESYAATRYIGLKDENTH
HHHCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCE
FLDLPFYETGQVKKNPLGQEDIEIVKEIIARIKPHQVFAAGDLADPHGTHEVCLNAIFAA
EEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHH
MKELKPEKYMDDCWLWLYRGAWHEWDIHEIDMAVPLSPSEVLLKRHAILYHQSQKDRVMF
HHHCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHEECCCCCEEEE
QGNDSREFWVRAEDRNKNTAILYDELGLAEYEAIEAFKRFDY
ECCCCCEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKTTTSLAYDIPGKFEETRFEKNHNVIFENSAEASKNVAQEIAELIRLKQTNNESCVLGL
CCCCCCEEECCCCCHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCEEEEE
ATGSSPIKVYEELVRMHKEEGLSFSNVITFNLDEYYPMNKENRQSYHYFMHQHLFNHIDI
ECCCCHHHHHHHHHHHHHHHCCCCHHEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCC
KPENVNIPDGTIALEQLNQYCIDYELKIKQAGGLDFQLLGIGRTGHVGFNEPGSHINSGT
CCCCCCCCCCHHHHHHHHHHHCCEEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCC
RIITLDHITRVDASSDFNGIDNVPKRAITMGVSTIMRSKRIVLMAWGQNKASIIKRTIQG
EEEEEECEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHC
EISSDVPATFLQNHPNATFVLDQSAASELTRFKTPWLVGECIWTEELKSKAIVWLCKKTK
CCCCCCCHHHHHCCCCEEEEECCHHHHHHHHCCCCCEEEHEEEHHHHHCCEEEEEEHHHH
QSILKLTDRDYNNNGMSDLLAHGSSAYDLNINMFNILQHTITGWPGGKPNTDDSHRPERA
HHHHHHHCCCCCCCCHHHHHHCCCCEEEEECHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
NPAKKRVILFSPHPDDDVISMGGTFSKLIKQGHDVHVVYQTSGNIAVTDDEALKFAEVCN
CCCCCEEEEECCCCCCCCEECCCHHHHHHHCCCCEEEEEEECCCEEECCHHHHHHHHHHH
DFVGADLPKDINFKSVIEFLNNKSENQIDSLEVRKLKGLIRRRESYAATRYIGLKDENTH
HHHCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCE
FLDLPFYETGQVKKNPLGQEDIEIVKEIIARIKPHQVFAAGDLADPHGTHEVCLNAIFAA
EEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHH
MKELKPEKYMDDCWLWLYRGAWHEWDIHEIDMAVPLSPSEVLLKRHAILYHQSQKDRVMF
HHHCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHEECCCCCEEEE
QGNDSREFWVRAEDRNKNTAILYDELGLAEYEAIEAFKRFDY
ECCCCCEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-glucosamine 6-phosphate; H2O

Specific reaction: D-glucosamine 6-phosphate + H2O = D-fructose 6-phosphate + NH3

General reaction: Group transfer (transmolecular Intramolecular oxidoreduction) [C]

Inhibitor: N3-(4-Methoxyfumaroyl)-L-2, 3-diaminopropanoic acid [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12663928 [H]