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The map label for this gene is hsrA [H]

Identifier: 146302532

GI number: 146302532

Start: 5806923

End: 5808329

Strand: Reverse

Name: hsrA [H]

Synonym: Fjoh_4805

Alternate gene names: 146302532

Gene position: 5808329-5806923 (Counterclockwise)

Preceding gene: 146302533

Following gene: 146302531

Centisome position: 95.27

GC content: 35.25

Gene sequence:

>1407_bases
ATGACAGTGATTACAGAGCAAAATAATGAAGCAATAAAAAAGGTATTACCCATTATTTTAGCGACTTCTATTTTTATGCA
GATGCTTGATTCGACAATTTTGAATACTTCACTGCCTTCTATTGCCAAAGATCTGCACGAATCACCGCTGGATATGCAGA
ACGCAATTATCAGTTATGTTTTGACTCTGGCGCTTTTTATGCCTGTGAGCGGTTTTTTATCAGATAAATTCGGGACTAAG
AAAGTTTTTATTGTCGCTTTGGTTTTGTTCAGTCTCGGATCCTTATTTTGTTCACTTTCTCAAAACCTGATGCATTTAGT
TTTAGCTCGAATTGTTCAGGGAGTGGGAGGAAGTTTAATGACTCCTGTAGGAAAGCTTGCTCTAATTAAAACATTTCCTA
AAAAAGAATTACTTAAAGCCATGAACTTTGCTATTATTCCCGCTTTAATTGGGCCGGTATTAGGGCCTTTGGTTGGAGGT
TATATGGTTGATTATTTATCCTGGCACTGGATTTTTTTAATAAACATTCCGTTTGGGCTTATCGGTATTCTTTTGAGTTT
AAAATATATGCCCAATTATAAATCTAAAATAATCGATTTTGATTTAAAAGGCTTTTTCATTTTTGCAGCGGCTTCGCTTT
TATTATCTATTTCATTAGAATTATTTGGCAATACCGTTCGGGTTTCACCTGTTTTAATGATTTTAATGTCGGGATTTTTA
ATGCTGTATTGGTACTATCGTCATGCATCGAAAGACAACAATCCTATTTTTCCTTTAAACCTGTTTCAGGTAAGAACATT
TCGAGTGGGTATTGTTGGTAATCTGGCTACACGTTTGGGAATCAGTTCTGTACCGTTGTTACTGCCCATGATGATCCAGA
TTGCTTATGGTCAGTCTGCAGTTGTGTCTGGGTGGATTGTAGCGCCAATGGCTTTGACGGCAATGTTTGGAAAATCAGCT
GTAATTGGGATTTTAAACACTTTTGGTTATCGAAAAACCTTAATGGTCAATACTTTTATTATTGGGTTTTTAATCTGCTG
TCTGGCTATTCCATCAATTCATACTTCAATTTATTGGTACGTTCCTATAATTTCGATTTTGGGTTTCTTTAACTCGATAC
AGTTTACCTCTATGAATTCTATTTCTATTGCCGATTTAAGACAGTATCATACCAGCAGCGGTAATTCTTTAATTTCAGTA
AATCAACAGCTGGCAATTGGTTTTGGAATCGCTTTCGGATTATTGGTTTTAAAGGTTTTTCAAGGAGATGTAAAACTTAT
TCATCACGAAATTCACAATGCATTTCGATATACTTTTCTGGTAGTGGGCTCTTTAACCATTTTATCCGGATTGGTTTTTA
GAAGATTGCATTTTAAAGACGGAGACAATTTAAAACCAGCGAATTAA

Upstream 100 bases:

>100_bases
CAAGAATGTTTTCGTACCTTTATTTTTAGGATGAAATAGAAGTTTTCGGGTCAATTTTTTTCCTGACAGAATAAAAAAAT
AAAGTCTTAAAATAAACAGG

Downstream 100 bases:

>100_bases
TTAAACCAAATCATAAATTAACTTAACCATTTCGCATAAACCTTAATAATGACTACAAACAATTCTTCAATTCCTATTTT
ACAAACCAAACAACATTTTG

Product: major facilitator transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: High-copy suppressor of rspA [H]

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MTVITEQNNEAIKKVLPIILATSIFMQMLDSTILNTSLPSIAKDLHESPLDMQNAIISYVLTLALFMPVSGFLSDKFGTK
KVFIVALVLFSLGSLFCSLSQNLMHLVLARIVQGVGGSLMTPVGKLALIKTFPKKELLKAMNFAIIPALIGPVLGPLVGG
YMVDYLSWHWIFLINIPFGLIGILLSLKYMPNYKSKIIDFDLKGFFIFAAASLLLSISLELFGNTVRVSPVLMILMSGFL
MLYWYYRHASKDNNPIFPLNLFQVRTFRVGIVGNLATRLGISSVPLLLPMMIQIAYGQSAVVSGWIVAPMALTAMFGKSA
VIGILNTFGYRKTLMVNTFIIGFLICCLAIPSIHTSIYWYVPIISILGFFNSIQFTSMNSISIADLRQYHTSSGNSLISV
NQQLAIGFGIAFGLLVLKVFQGDVKLIHHEIHNAFRYTFLVVGSLTILSGLVFRRLHFKDGDNLKPAN

Sequences:

>Translated_468_residues
MTVITEQNNEAIKKVLPIILATSIFMQMLDSTILNTSLPSIAKDLHESPLDMQNAIISYVLTLALFMPVSGFLSDKFGTK
KVFIVALVLFSLGSLFCSLSQNLMHLVLARIVQGVGGSLMTPVGKLALIKTFPKKELLKAMNFAIIPALIGPVLGPLVGG
YMVDYLSWHWIFLINIPFGLIGILLSLKYMPNYKSKIIDFDLKGFFIFAAASLLLSISLELFGNTVRVSPVLMILMSGFL
MLYWYYRHASKDNNPIFPLNLFQVRTFRVGIVGNLATRLGISSVPLLLPMMIQIAYGQSAVVSGWIVAPMALTAMFGKSA
VIGILNTFGYRKTLMVNTFIIGFLICCLAIPSIHTSIYWYVPIISILGFFNSIQFTSMNSISIADLRQYHTSSGNSLISV
NQQLAIGFGIAFGLLVLKVFQGDVKLIHHEIHNAFRYTFLVVGSLTILSGLVFRRLHFKDGDNLKPAN
>Mature_467_residues
TVITEQNNEAIKKVLPIILATSIFMQMLDSTILNTSLPSIAKDLHESPLDMQNAIISYVLTLALFMPVSGFLSDKFGTKK
VFIVALVLFSLGSLFCSLSQNLMHLVLARIVQGVGGSLMTPVGKLALIKTFPKKELLKAMNFAIIPALIGPVLGPLVGGY
MVDYLSWHWIFLINIPFGLIGILLSLKYMPNYKSKIIDFDLKGFFIFAAASLLLSISLELFGNTVRVSPVLMILMSGFLM
LYWYYRHASKDNNPIFPLNLFQVRTFRVGIVGNLATRLGISSVPLLLPMMIQIAYGQSAVVSGWIVAPMALTAMFGKSAV
IGILNTFGYRKTLMVNTFIIGFLICCLAIPSIHTSIYWYVPIISILGFFNSIQFTSMNSISIADLRQYHTSSGNSLISVN
QQLAIGFGIAFGLLVLKVFQGDVKLIHHEIHNAFRYTFLVVGSLTILSGLVFRRLHFKDGDNLKPAN

Specific function: Unknown

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=450, Percent_Identity=45.7777777777778, Blast_Score=412, Evalue=1e-116,
Organism=Escherichia coli, GI1788392, Length=450, Percent_Identity=40.4444444444444, Blast_Score=302, Evalue=3e-83,
Organism=Escherichia coli, GI1788710, Length=303, Percent_Identity=31.6831683168317, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI87081983, Length=294, Percent_Identity=28.5714285714286, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1789042, Length=308, Percent_Identity=28.2467532467532, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1788509, Length=136, Percent_Identity=27.9411764705882, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1790794, Length=243, Percent_Identity=25.1028806584362, Blast_Score=62, Evalue=1e-10,
Organism=Saccharomyces cerevisiae, GI6323735, Length=205, Percent_Identity=27.8048780487805, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6325455, Length=160, Percent_Identity=26.875, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 51710; Mature: 51579

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVITEQNNEAIKKVLPIILATSIFMQMLDSTILNTSLPSIAKDLHESPLDMQNAIISYV
CCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHH
LTLALFMPVSGFLSDKFGTKKVFIVALVLFSLGSLFCSLSQNLMHLVLARIVQGVGGSLM
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
TPVGKLALIKTFPKKELLKAMNFAIIPALIGPVLGPLVGGYMVDYLSWHWIFLINIPFGL
CCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCHHH
IGILLSLKYMPNYKSKIIDFDLKGFFIFAAASLLLSISLELFGNTVRVSPVLMILMSGFL
HHHHHHHHCCCCCHHHEEEECHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
MLYWYYRHASKDNNPIFPLNLFQVRTFRVGIVGNLATRLGISSVPLLLPMMIQIAYGQSA
HHHHHHHHCCCCCCCCCCEEHHEEHHEEEHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHH
VVSGWIVAPMALTAMFGKSAVIGILNTFGYRKTLMVNTFIIGFLICCLAIPSIHTSIYWY
HHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VPIISILGFFNSIQFTSMNSISIADLRQYHTSSGNSLISVNQQLAIGFGIAFGLLVLKVF
HHHHHHHHHHCCCEECCCCCCHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHH
QGDVKLIHHEIHNAFRYTFLVVGSLTILSGLVFRRLHFKDGDNLKPAN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TVITEQNNEAIKKVLPIILATSIFMQMLDSTILNTSLPSIAKDLHESPLDMQNAIISYV
CEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHH
LTLALFMPVSGFLSDKFGTKKVFIVALVLFSLGSLFCSLSQNLMHLVLARIVQGVGGSLM
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
TPVGKLALIKTFPKKELLKAMNFAIIPALIGPVLGPLVGGYMVDYLSWHWIFLINIPFGL
CCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCHHH
IGILLSLKYMPNYKSKIIDFDLKGFFIFAAASLLLSISLELFGNTVRVSPVLMILMSGFL
HHHHHHHHCCCCCHHHEEEECHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
MLYWYYRHASKDNNPIFPLNLFQVRTFRVGIVGNLATRLGISSVPLLLPMMIQIAYGQSA
HHHHHHHHCCCCCCCCCCEEHHEEHHEEEHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHH
VVSGWIVAPMALTAMFGKSAVIGILNTFGYRKTLMVNTFIIGFLICCLAIPSIHTSIYWY
HHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VPIISILGFFNSIQFTSMNSISIADLRQYHTSSGNSLISVNQQLAIGFGIAFGLLVLKVF
HHHHHHHHHHCCCEECCCCCCHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHH
QGDVKLIHHEIHNAFRYTFLVVGSLTILSGLVFRRLHFKDGDNLKPAN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]