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The map label for this gene is nasA [H]

Identifier: 146302356

GI number: 146302356

Start: 5595358

End: 5596842

Strand: Direct

Name: nasA [H]

Synonym: Fjoh_4629

Alternate gene names: 146302356

Gene position: 5595358-5596842 (Clockwise)

Preceding gene: 146302355

Following gene: 146302357

Centisome position: 91.77

GC content: 37.44

Gene sequence:

>1485_bases
ATGAAAAATACAAACTCACTATCGCAATCACACAAAATTTTAGTTTTGAACACATTGGCTTTTACAGTGTGTTTTGCCTG
CTGGACATTAAACGGGGTTCTGGTAACTTTTTTAGTCGATAACGGAATTTTCCATTGGGATGTTATTCAAGTCGGATGGC
TTTTGGGTATTCCAATTTTAACCGGATCAATAATGCGTCTGCCAATTGGCATCTTAACCGATAAATTTGGCGGAAAATAT
GTTTTTTCACTTTTACTGCTTCTAAGTTCAATTCCGCTGTTCCTCCTTCCCTACGCTGATAGTTTCGCCATGTTTGCTTT
ACTAAGTTTTTTATTCGGAATGGTCGGAACGAGTTTTGCTGTCGGAATTGGTTACACTTCTATCTGGTATCCAAAAGAAT
GGCAGGGACGCGCGCTCGGAATATTCGGAATGGGAAATGCTGGAGCGGCAATAACTACTTTTCTTGCTCCTTCCTTATTA
AATCATTTCTCTATTGATGATCCGCAAAACGGCTGGAAAGTGCTTCCTGTTATTTATGCAGTTTCATTAGTCGTAATTGG
TATTGCGTTTTTAATTTTCGCTCAAAATAAAAAAATAGAAAGCAGCGGTAAAGCAATTTCGCAAATGCTTACACCTTTAA
AATCACAAAGAGTTTGGCGTTTTGGAGCTTATTATTTCTTAGTTTTTGGATGCTTTGTAGCATATTCGCAATGGTTGCTG
CCAAATTTCATGAACGTTTACCAAACGAGTTTAGTTATGGGAGGTTTGTTTGCCACGATGTTCAGTTTACCTTCTGGTGT
TATTAGAGCTTTTGGAGGTTATTTATCAGATAAATTCGGAGCGAGAAAAGTAATGTACTGGGTTTTGGGTTCTTCAGTAA
TTTTAAGTGCTTTTTTATCGATTCCAAAAATGGAAATAACTACTTCTGGTCCGGGAATTCTGGCTGCTAAAAAAGGAGTC
GTAAACGAAATCAGTGACAAAACCGTAAAAGTTGGCGATAAAGAATATTCAATTGCACAAAAAACAGCCAATACAGCCGA
AAATGCCATTTTCCCTACTACTTCGAGCTGGCAGGATGTAGTTGTTGCTCACAATCAAAGTGTTGCTAAAAAAGAATTAA
TTGCCAAAGGCGTAACGGTTATTAAGTTTGATGCCAATATGTGGGTTTTCTTAGTCCTAGTTATTCTAATCGGGATTTCC
TGGGGAATTGGGAAAGCGGCAGTTTACAAACACATTCCTGAATATTTTCCTAATGAAGTTGGCGTTGTAGGCGGCATGGT
TGGAATGATTGGCGGTTTAGGCGGATTCTTTGGCCCAATTATATTTGGATATTTACTAACAGCAACCGGAATCTGGTCAA
GTTCATGGATTTTTATTTTAATCTTTTCAACTGTATGTTTGGTTTGGATGCATTATACTATAACACAACTATCTAGAGAG
AAACAAGTTAAAGTAACTAAAGCAAAATTAGAACCAGCATATTAA

Upstream 100 bases:

>100_bases
TAAAAAATACGTATAAATACGTAATTATTTTTCAAAAACTGCGTTTTAGCAGGTTTTAACTAAGTTATAGTACTTATTTT
TACATAAAAATATAAGTGCT

Downstream 100 bases:

>100_bases
ACTTAGATTAAAAAAAGATTAGAAATATGACTGCGATCATAGTATTCGACGGCATTTTCTTATAAATTTACCCTCGATAA
AAATTTACCAATTTTATGAA

Product: major facilitator transporter

Products: Proton [Cytoplasm]; nitrite [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MKNTNSLSQSHKILVLNTLAFTVCFACWTLNGVLVTFLVDNGIFHWDVIQVGWLLGIPILTGSIMRLPIGILTDKFGGKY
VFSLLLLLSSIPLFLLPYADSFAMFALLSFLFGMVGTSFAVGIGYTSIWYPKEWQGRALGIFGMGNAGAAITTFLAPSLL
NHFSIDDPQNGWKVLPVIYAVSLVVIGIAFLIFAQNKKIESSGKAISQMLTPLKSQRVWRFGAYYFLVFGCFVAYSQWLL
PNFMNVYQTSLVMGGLFATMFSLPSGVIRAFGGYLSDKFGARKVMYWVLGSSVILSAFLSIPKMEITTSGPGILAAKKGV
VNEISDKTVKVGDKEYSIAQKTANTAENAIFPTTSSWQDVVVAHNQSVAKKELIAKGVTVIKFDANMWVFLVLVILIGIS
WGIGKAAVYKHIPEYFPNEVGVVGGMVGMIGGLGGFFGPIIFGYLLTATGIWSSSWIFILIFSTVCLVWMHYTITQLSRE
KQVKVTKAKLEPAY

Sequences:

>Translated_494_residues
MKNTNSLSQSHKILVLNTLAFTVCFACWTLNGVLVTFLVDNGIFHWDVIQVGWLLGIPILTGSIMRLPIGILTDKFGGKY
VFSLLLLLSSIPLFLLPYADSFAMFALLSFLFGMVGTSFAVGIGYTSIWYPKEWQGRALGIFGMGNAGAAITTFLAPSLL
NHFSIDDPQNGWKVLPVIYAVSLVVIGIAFLIFAQNKKIESSGKAISQMLTPLKSQRVWRFGAYYFLVFGCFVAYSQWLL
PNFMNVYQTSLVMGGLFATMFSLPSGVIRAFGGYLSDKFGARKVMYWVLGSSVILSAFLSIPKMEITTSGPGILAAKKGV
VNEISDKTVKVGDKEYSIAQKTANTAENAIFPTTSSWQDVVVAHNQSVAKKELIAKGVTVIKFDANMWVFLVLVILIGIS
WGIGKAAVYKHIPEYFPNEVGVVGGMVGMIGGLGGFFGPIIFGYLLTATGIWSSSWIFILIFSTVCLVWMHYTITQLSRE
KQVKVTKAKLEPAY
>Mature_494_residues
MKNTNSLSQSHKILVLNTLAFTVCFACWTLNGVLVTFLVDNGIFHWDVIQVGWLLGIPILTGSIMRLPIGILTDKFGGKY
VFSLLLLLSSIPLFLLPYADSFAMFALLSFLFGMVGTSFAVGIGYTSIWYPKEWQGRALGIFGMGNAGAAITTFLAPSLL
NHFSIDDPQNGWKVLPVIYAVSLVVIGIAFLIFAQNKKIESSGKAISQMLTPLKSQRVWRFGAYYFLVFGCFVAYSQWLL
PNFMNVYQTSLVMGGLFATMFSLPSGVIRAFGGYLSDKFGARKVMYWVLGSSVILSAFLSIPKMEITTSGPGILAAKKGV
VNEISDKTVKVGDKEYSIAQKTANTAENAIFPTTSSWQDVVVAHNQSVAKKELIAKGVTVIKFDANMWVFLVLVILIGIS
WGIGKAAVYKHIPEYFPNEVGVVGGMVGMIGGLGGFFGPIIFGYLLTATGIWSSSWIFILIFSTVCLVWMHYTITQLSRE
KQVKVTKAKLEPAY

Specific function: May function as a nitrate transporter [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 54127; Mature: 54127

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNTNSLSQSHKILVLNTLAFTVCFACWTLNGVLVTFLVDNGIFHWDVIQVGWLLGIPIL
CCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHH
TGSIMRLPIGILTDKFGGKYVFSLLLLLSSIPLFLLPYADSFAMFALLSFLFGMVGTSFA
HCHHHHCCHHHHHCCCCHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHH
VGIGYTSIWYPKEWQGRALGIFGMGNAGAAITTFLAPSLLNHFSIDDPQNGWKVLPVIYA
HHCCCCEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
VSLVVIGIAFLIFAQNKKIESSGKAISQMLTPLKSQRVWRFGAYYFLVFGCFVAYSQWLL
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PNFMNVYQTSLVMGGLFATMFSLPSGVIRAFGGYLSDKFGARKVMYWVLGSSVILSAFLS
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHC
IPKMEITTSGPGILAAKKGVVNEISDKTVKVGDKEYSIAQKTANTAENAIFPTTSSWQDV
CCCEEEECCCCCEEEECCCHHHHHCCCEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCE
VVAHNQSVAKKELIAKGVTVIKFDANMWVFLVLVILIGISWGIGKAAVYKHIPEYFPNEV
EEECCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHH
GVVGGMVGMIGGLGGFFGPIIFGYLLTATGIWSSSWIFILIFSTVCLVWMHYTITQLSRE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KQVKVTKAKLEPAY
HHHHHHHHHCCCCC
>Mature Secondary Structure
MKNTNSLSQSHKILVLNTLAFTVCFACWTLNGVLVTFLVDNGIFHWDVIQVGWLLGIPIL
CCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHH
TGSIMRLPIGILTDKFGGKYVFSLLLLLSSIPLFLLPYADSFAMFALLSFLFGMVGTSFA
HCHHHHCCHHHHHCCCCHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHH
VGIGYTSIWYPKEWQGRALGIFGMGNAGAAITTFLAPSLLNHFSIDDPQNGWKVLPVIYA
HHCCCCEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
VSLVVIGIAFLIFAQNKKIESSGKAISQMLTPLKSQRVWRFGAYYFLVFGCFVAYSQWLL
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PNFMNVYQTSLVMGGLFATMFSLPSGVIRAFGGYLSDKFGARKVMYWVLGSSVILSAFLS
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHC
IPKMEITTSGPGILAAKKGVVNEISDKTVKVGDKEYSIAQKTANTAENAIFPTTSSWQDV
CCCEEEECCCCCEEEECCCHHHHHCCCEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCE
VVAHNQSVAKKELIAKGVTVIKFDANMWVFLVLVILIGISWGIGKAAVYKHIPEYFPNEV
EEECCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHH
GVVGGMVGMIGGLGGFFGPIIFGYLLTATGIWSSSWIFILIFSTVCLVWMHYTITQLSRE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KQVKVTKAKLEPAY
HHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: nitrite [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: nitrite [Cytoplasm] + Proton [Periplasm] = Proton [Cytoplasm] + nitrite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]