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The map label for this gene is trmH [C]

Identifier: 146302323

GI number: 146302323

Start: 5559410

End: 5559907

Strand: Direct

Name: trmH [C]

Synonym: Fjoh_4596

Alternate gene names: 146302323

Gene position: 5559410-5559907 (Clockwise)

Preceding gene: 146302322

Following gene: 146302324

Centisome position: 91.18

GC content: 32.13

Gene sequence:

>498_bases
ATGCAGCTGACTCACGAAGAAAATCAATTTGAGAGAAAAACATTTCCAATAACTTTGGTTTGCGATCATATTTATTTTCA
GCAGAATATTGGTTCGCTTTTTAGAATTTCAGAAGCTTTTGGTGTAGAAAATATTATATTTTTAGGAAAAGACATTCCGC
TTACGCCAAGAAAAATCAACAAAACTTCACGCAGTACACATCTCCATGTACCGCATCAAATTATTGAAGAAACAACAGAA
TTAACTGCTTATCTTCAAGAAAACAACTTCGAAATCATTGCTTTAGAAATTACAAGCAACAGTAAACCTCTGAAAGAAGT
TCTAATTCCAAAGGATAAAAAAACGGCACTTTTGATAGGAAGTGAAATTGCCGGAATTTCAGAAGAACTATTAAAAATTT
CACATCAAATTGTTCACATTAATATGTTTGGAAAAAACTCAAGCATGAATGTAGTACAAGCTGCCAGCATTGCTTTGTAT
GAGATTACTTCTTTGTAA

Upstream 100 bases:

>100_bases
AAGTGATTAGTAACTAGTAATTCATCAAATTAAAAAAGAAAATTATTTAGATTCTAAGTCACCACTAATTACTTTTCACT
AATTACTTTTCACTAAAAAA

Downstream 100 bases:

>100_bases
TTTTTTGTTAAATTTTATAGAATAATGGGTTCGATTTTCGAATAATTATATAAACAAACTTTCAAAACCTCGACAAAAAG
CAAATAACAACGTTAAAATG

Product: tRNA/rRNA methyltransferase SpoU

Products: S-adenosyl-L-homocysteine; tRNATyr Containing 2'-O-methylguanosine [C]

Alternate protein names: SpoU RRNA Methylase Family Protein; RNA MethylaU Family; TrmH Family TRNA/RRNA Methyltransferase; TRNA/RRNA MethyltransferaU; rRNA Methyltransferase; rRNA Methylases; RNA Methyltransferase TrmH Family; TrmH Family RNA Methyltransferase; TRNA/RRNA Methyltransferase Protein; RNA Methylase; RNA Methyltransferase; TRNA-Methyltransferase

Number of amino acids: Translated: 165; Mature: 165

Protein sequence:

>165_residues
MQLTHEENQFERKTFPITLVCDHIYFQQNIGSLFRISEAFGVENIIFLGKDIPLTPRKINKTSRSTHLHVPHQIIEETTE
LTAYLQENNFEIIALEITSNSKPLKEVLIPKDKKTALLIGSEIAGISEELLKISHQIVHINMFGKNSSMNVVQAASIALY
EITSL

Sequences:

>Translated_165_residues
MQLTHEENQFERKTFPITLVCDHIYFQQNIGSLFRISEAFGVENIIFLGKDIPLTPRKINKTSRSTHLHVPHQIIEETTE
LTAYLQENNFEIIALEITSNSKPLKEVLIPKDKKTALLIGSEIAGISEELLKISHQIVHINMFGKNSSMNVVQAASIALY
EITSL
>Mature_165_residues
MQLTHEENQFERKTFPITLVCDHIYFQQNIGSLFRISEAFGVENIIFLGKDIPLTPRKINKTSRSTHLHVPHQIIEETTE
LTAYLQENNFEIIALEITSNSKPLKEVLIPKDKKTALLIGSEIAGISEELLKISHQIVHINMFGKNSSMNVVQAASIALY
EITSL

Specific function: Specifically Methylates Guanosine-18 In Various tRNAs. [C]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.1.1.34 [C]

Molecular weight: Translated: 18700; Mature: 18700

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLTHEENQFERKTFPITLVCDHIYFQQNIGSLFRISEAFGVENIIFLGKDIPLTPRKIN
CCCCCCCCCCHHHCCCEEEEEEHHHHHHCCCHHHHHHHHCCCCEEEEECCCCCCCCHHCC
KTSRSTHLHVPHQIIEETTELTAYLQENNFEIIALEITSNSKPLKEVLIPKDKKTALLIG
CCCCCEEEECCHHHHHHHHHHHEEEECCCEEEEEEEECCCCCCHHHHCCCCCCCEEEEEC
SEIAGISEELLKISHQIVHINMFGKNSSMNVVQAASIALYEITSL
CCCCCHHHHHHHHHEEEEEEEEECCCCCCCHHHHHEEEEEEEECC
>Mature Secondary Structure
MQLTHEENQFERKTFPITLVCDHIYFQQNIGSLFRISEAFGVENIIFLGKDIPLTPRKIN
CCCCCCCCCCHHHCCCEEEEEEHHHHHHCCCHHHHHHHHCCCCEEEEECCCCCCCCHHCC
KTSRSTHLHVPHQIIEETTELTAYLQENNFEIIALEITSNSKPLKEVLIPKDKKTALLIG
CCCCCEEEECCHHHHHHHHHHHEEEECCCEEEEEEEECCCCCCHHHHCCCCCCCEEEEEC
SEIAGISEELLKISHQIVHINMFGKNSSMNVVQAASIALYEITSL
CCCCCHHHHHHHHHEEEEEEEEECCCCCCCHHHHHEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; tRNATyr [C]

Specific reaction: S-Adenosyl-L-methionine + tRNATyr = S-adenosyl-L-homocysteine + tRNATyr Containing 2'-O-methylguanosine [C]

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA