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The map label for this gene is nfnB [C]

Identifier: 146302215

GI number: 146302215

Start: 5411257

End: 5411889

Strand: Direct

Name: nfnB [C]

Synonym: Fjoh_4487

Alternate gene names: 146302215

Gene position: 5411257-5411889 (Clockwise)

Preceding gene: 146302214

Following gene: 146302216

Centisome position: 88.75

GC content: 36.81

Gene sequence:

>633_bases
ATGAAATTACTAGATGCTCTAAAATGGCGCTATGCAGCCAAAAAAATGAACGGACAAGTTGTACCGCAGGAAAAAATCGA
TTATATACTAGAAGCTGCAAGATTAGCTCCTTCTTCATCTGGTTTACAGCCATATAAAATTTTTGTAATTACGAATAAGG
AATTACAGGAAAAAATAAAAACTGTAGGTTTTGAACAAAGCCAAATTGTGGATGCTTCTCACGTATTGGTTTTTGCGGCA
TGGGATGGATACTCTTTAGAAAAAATATCAGAAGTTTTTGAAAGAACAACGGCTGAAAGAGGAATTCCGGCAAGTGCTAT
GGATGAATATAAAGAAAGACTTTGGGGAATGTATAAAGATCTTGGTCAGGAATGGCATGCCAATCATGCAGCAAAACAAG
CGTACATTTCGTTTGGTTTAGCTATTGCTGCAGCTGCAGAACAAGAGGTTGATGCTACACCAATGGAAGGTTTTATCCCA
GCCGAGGTAGATAAATTATTAGGTTTAAACGAGCTTGGTCTTAAAAGTGTATTAGTACTGCCTTTGGGTTACAGAGATGC
TTCTAACGATTGGTTAGTGAACCTAAAAAAAGTAAGAACTCCGCAAAGCGAATTCATTACTGAAATAAAATAA

Upstream 100 bases:

>100_bases
GGATAAGATTATCTTTATATTAACATTAGATTTTCTCTTGAGATTATAAAAATATATTTAAAGTCTTTACTTTTGAATAG
AATTAAAATTGATACAGAAA

Downstream 100 bases:

>100_bases
TTTCATTTCAAACATACCATAAACCAATAATTGAATCACTGCCGCATGAACGGTATATCTTTTTTGGCTTGAAATGTGGA
TTTACCCATTTCAGCTTATT

Product: nitroreductase

Products: NAD(P)(+); @TETR01.txt*5,6,7,8-Tetrahydropteridine!. [C]

Alternate protein names: NA

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MKLLDALKWRYAAKKMNGQVVPQEKIDYILEAARLAPSSSGLQPYKIFVITNKELQEKIKTVGFEQSQIVDASHVLVFAA
WDGYSLEKISEVFERTTAERGIPASAMDEYKERLWGMYKDLGQEWHANHAAKQAYISFGLAIAAAAEQEVDATPMEGFIP
AEVDKLLGLNELGLKSVLVLPLGYRDASNDWLVNLKKVRTPQSEFITEIK

Sequences:

>Translated_210_residues
MKLLDALKWRYAAKKMNGQVVPQEKIDYILEAARLAPSSSGLQPYKIFVITNKELQEKIKTVGFEQSQIVDASHVLVFAA
WDGYSLEKISEVFERTTAERGIPASAMDEYKERLWGMYKDLGQEWHANHAAKQAYISFGLAIAAAAEQEVDATPMEGFIP
AEVDKLLGLNELGLKSVLVLPLGYRDASNDWLVNLKKVRTPQSEFITEIK
>Mature_210_residues
MKLLDALKWRYAAKKMNGQVVPQEKIDYILEAARLAPSSSGLQPYKIFVITNKELQEKIKTVGFEQSQIVDASHVLVFAA
WDGYSLEKISEVFERTTAERGIPASAMDEYKERLWGMYKDLGQEWHANHAAKQAYISFGLAIAAAAEQEVDATPMEGFIP
AEVDKLLGLNELGLKSVLVLPLGYRDASNDWLVNLKKVRTPQSEFITEIK

Specific function: Reduction Of A Variety Of Nitroaromatic Compounds Using NADH (And To Lesser Extent Nadph) As Source Of Reducing Equivalents; Two Electrons Are Transferred. Capable Of Reducing Nitrofurazone, Quinones And The Anti-Tumor Agent Cb1954 (5- (Aziridin-1-Yl)-2,4

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family [H]

Homologues:

Organism=Escherichia coli, GI1786792, Length=220, Percent_Identity=29.5454545454545, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 23591; Mature: 23591

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLDALKWRYAAKKMNGQVVPQEKIDYILEAARLAPSSSGLQPYKIFVITNKELQEKIK
CCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEEECHHHHHHHH
TVGFEQSQIVDASHVLVFAAWDGYSLEKISEVFERTTAERGIPASAMDEYKERLWGMYKD
HCCCCHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LGQEWHANHAAKQAYISFGLAIAAAAEQEVDATPMEGFIPAEVDKLLGLNELGLKSVLVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCHHCCCEEEEE
PLGYRDASNDWLVNLKKVRTPQSEFITEIK
ECCCCCCCCCCEEEHHHHCCCHHHHHHCCC
>Mature Secondary Structure
MKLLDALKWRYAAKKMNGQVVPQEKIDYILEAARLAPSSSGLQPYKIFVITNKELQEKIK
CCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEEECHHHHHHHH
TVGFEQSQIVDASHVLVFAAWDGYSLEKISEVFERTTAERGIPASAMDEYKERLWGMYKD
HCCCCHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LGQEWHANHAAKQAYISFGLAIAAAAEQEVDATPMEGFIPAEVDKLLGLNELGLKSVLVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCHHCCCEEEEE
PLGYRDASNDWLVNLKKVRTPQSEFITEIK
ECCCCCCCCCCEEEHHHHCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H; 6,7-Dihydropteridine [C]

Specific reaction: NAD(P)H + 6,7-Dihydropteridine = NAD(P)(+) + @TETR01.txt*5,6,7,8-Tetrahydropteridine!. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA