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The map label for this gene is kojP [H]

Identifier: 146302160

GI number: 146302160

Start: 5337222

End: 5339228

Strand: Reverse

Name: kojP [H]

Synonym: Fjoh_4428

Alternate gene names: 146302160

Gene position: 5339228-5337222 (Counterclockwise)

Preceding gene: 146302161

Following gene: 146302149

Centisome position: 87.57

GC content: 39.76

Gene sequence:

>2007_bases
ATGCTATGCGGCAGCAATTATTTGTATAGCCAGGATCCGTGGAAGCTTTCGGCAGATAAACCGGATTCCAATAATTATTA
TGGAGAAACGGTGGCTAATGGCATGATCGGAATTATATCGTCACCGGAGCCTTTAAAAGTGAAAGAAGTAGTATTGGCAG
GAACGTACGATATCTACAAGCGTGGAAGGGTCAGTAGTTTCATTCCAAATTACAATCTGCTGAATATGAAACTGGCTTTT
AATGGTGAATCTGTGCAGACCTATAATATTAATAATTATAAGCAGGAACTGGATATGCGCAATGGGGCTTTCACGGGGTC
TTTTCAATTTAAGGATTTAGCAACTGTAACATATTCATACTATGCTTTGCGTCATCTTCCGCATTGTATAATGATGGTTG
TCAATATAAATACGCAAAAAGATACCGAAATCAACGTAGAGAATCTTTTGGAGACTCCCTCTTCACTTAACAACCAGCAG
AATTATTTTCAGAATATTACAAATACCCATGTTAATATACCATTATTGACTTCTGTTGCATTTACGCCCACAGGAAGATC
TAAAATAGCTGTTTCAAATACATTCCTTTTTGATGAAGGGAAAAAGCTGCAACCTGAGATCCTGCACAGAATGAATGACG
CTGATATGCATGCAATGTCATTTGACAAAAAAATAAAAGCGGGCAAGACTTATTCTTTTGCTTTGATAGGCAGTCTTATA
TCATCAGATCATATAAATGATCCTTATAATGAAGCTGAAAGGCTGACTATTTATGCAGCCTTAGAAGGAAAGTCAAGGCT
TTTAAACAGACATATGCAAGAATGGAATAGCCTCTGGCAGAGTGATATCCAAGTAGAAGGAGATCCCCAAGCGCAACAGG
ATATAAGAAGCATGCTTTATCATCTCTATTCTTTCACAAGAAAAAGCACATCTCTTTCTCCATCGCCGATGGGGCTGAGC
GGGCTTGGATATAACGGACATGTGTTTTGGGATACCGAAATCTGGATGTTTCCTCCCATGCTGCTGCTTCATCCTGAAAT
AGCAAAAAGCATGATTGAATATCGTTATCAGAGGCTGGATGCAGCACGTAAAAAAGCTGCCATTTATGGATATGATGGCG
CAATGTTTCCCTGGGAAAGTGCAGATTCCGGAGCAGAAGAAACTCCGGTGAATGCTTTAACGGGAGCTTTTGAGCATCAT
GTAACAGGAGATGTGGCCATTGCTGCCTGGCAGTATTATCTTGTTACAGGTGACAAGGAATGGCTGAAAGAAAAAGGGTG
GCCGATATTAAAAGCAACTGCTGAATTTTGGGCGAGCCGAGTAGAGAAGAATGATAAAGGAGAATATGAAATAAAGAATG
TCGTTGCAGCAGATGAATGGGCTGAAAATATTGACAACAATGCTTATACGAACGGAACAGCTATCAGAAATCTGCAGTAT
GCATCTAAATGTGCAACTGTATTGGGTGTCATAGCTCCCAAAGAGTGGACTTTGATTGCAGATAAAATTCTAATTTCGAA
GATGAGTAATGGAGTAACCAGAGAGCACGATAGCTATACGGATCAGAATATCAAGCAGGCCGATGCCAATCTATTAGCAT
ATCCATTGAAGCTTATAACCGATAAAGAACAGATAGAAAGGGATTTGAAATATTATCAAACTAAAATTCCCCAGTCAGAC
ACTCCCGCTATGACACAAGCTATATTTTCATTACTCTACAGCCGCTTAGAGGATAGCGACCAGGCTTATCATTGGTTTAA
AGATGCTTATCAACCGAACCTTAACCCTCCTTTCAGGGTCATTTCAGAATGCAAGGGAGGGACAAACCCATACTTTTCGA
CAGGTGCAGGAGGTGTTCTTCAAGCCGTAATAATGGGATTTGGAGGTTTGGATATTGATGCTGCCGGAGGCATAAAACAA
GTTAAATCAGTATTGCCTAAGAACTGGAAAAAATTAACAATAACCGGAATCGGAATAGAAAAAAAGACTTTTGTACTAAC
TCATTAA

Upstream 100 bases:

>100_bases
ACAGACTGCAGCGATCTTTATGTATTATAACTAACCTCTTGAATATTTAACCTTAAGTAAGATGAAAAAATACATATTTA
ATCATGTTTTCTTTTTTTTG

Downstream 100 bases:

>100_bases
AAATAGTAATAGGAGCCGAGGAGATAACTAAGATAAAAAAGTTTCAAGACAAGAGTTTGAGGTTAATTACCGTTAACCTC
TTGTCTTTTTTATGTAGTTT

Product: glycoside hydrolase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 668; Mature: 668

Protein sequence:

>668_residues
MLCGSNYLYSQDPWKLSADKPDSNNYYGETVANGMIGIISSPEPLKVKEVVLAGTYDIYKRGRVSSFIPNYNLLNMKLAF
NGESVQTYNINNYKQELDMRNGAFTGSFQFKDLATVTYSYYALRHLPHCIMMVVNINTQKDTEINVENLLETPSSLNNQQ
NYFQNITNTHVNIPLLTSVAFTPTGRSKIAVSNTFLFDEGKKLQPEILHRMNDADMHAMSFDKKIKAGKTYSFALIGSLI
SSDHINDPYNEAERLTIYAALEGKSRLLNRHMQEWNSLWQSDIQVEGDPQAQQDIRSMLYHLYSFTRKSTSLSPSPMGLS
GLGYNGHVFWDTEIWMFPPMLLLHPEIAKSMIEYRYQRLDAARKKAAIYGYDGAMFPWESADSGAEETPVNALTGAFEHH
VTGDVAIAAWQYYLVTGDKEWLKEKGWPILKATAEFWASRVEKNDKGEYEIKNVVAADEWAENIDNNAYTNGTAIRNLQY
ASKCATVLGVIAPKEWTLIADKILISKMSNGVTREHDSYTDQNIKQADANLLAYPLKLITDKEQIERDLKYYQTKIPQSD
TPAMTQAIFSLLYSRLEDSDQAYHWFKDAYQPNLNPPFRVISECKGGTNPYFSTGAGGVLQAVIMGFGGLDIDAAGGIKQ
VKSVLPKNWKKLTITGIGIEKKTFVLTH

Sequences:

>Translated_668_residues
MLCGSNYLYSQDPWKLSADKPDSNNYYGETVANGMIGIISSPEPLKVKEVVLAGTYDIYKRGRVSSFIPNYNLLNMKLAF
NGESVQTYNINNYKQELDMRNGAFTGSFQFKDLATVTYSYYALRHLPHCIMMVVNINTQKDTEINVENLLETPSSLNNQQ
NYFQNITNTHVNIPLLTSVAFTPTGRSKIAVSNTFLFDEGKKLQPEILHRMNDADMHAMSFDKKIKAGKTYSFALIGSLI
SSDHINDPYNEAERLTIYAALEGKSRLLNRHMQEWNSLWQSDIQVEGDPQAQQDIRSMLYHLYSFTRKSTSLSPSPMGLS
GLGYNGHVFWDTEIWMFPPMLLLHPEIAKSMIEYRYQRLDAARKKAAIYGYDGAMFPWESADSGAEETPVNALTGAFEHH
VTGDVAIAAWQYYLVTGDKEWLKEKGWPILKATAEFWASRVEKNDKGEYEIKNVVAADEWAENIDNNAYTNGTAIRNLQY
ASKCATVLGVIAPKEWTLIADKILISKMSNGVTREHDSYTDQNIKQADANLLAYPLKLITDKEQIERDLKYYQTKIPQSD
TPAMTQAIFSLLYSRLEDSDQAYHWFKDAYQPNLNPPFRVISECKGGTNPYFSTGAGGVLQAVIMGFGGLDIDAAGGIKQ
VKSVLPKNWKKLTITGIGIEKKTFVLTH
>Mature_668_residues
MLCGSNYLYSQDPWKLSADKPDSNNYYGETVANGMIGIISSPEPLKVKEVVLAGTYDIYKRGRVSSFIPNYNLLNMKLAF
NGESVQTYNINNYKQELDMRNGAFTGSFQFKDLATVTYSYYALRHLPHCIMMVVNINTQKDTEINVENLLETPSSLNNQQ
NYFQNITNTHVNIPLLTSVAFTPTGRSKIAVSNTFLFDEGKKLQPEILHRMNDADMHAMSFDKKIKAGKTYSFALIGSLI
SSDHINDPYNEAERLTIYAALEGKSRLLNRHMQEWNSLWQSDIQVEGDPQAQQDIRSMLYHLYSFTRKSTSLSPSPMGLS
GLGYNGHVFWDTEIWMFPPMLLLHPEIAKSMIEYRYQRLDAARKKAAIYGYDGAMFPWESADSGAEETPVNALTGAFEHH
VTGDVAIAAWQYYLVTGDKEWLKEKGWPILKATAEFWASRVEKNDKGEYEIKNVVAADEWAENIDNNAYTNGTAIRNLQY
ASKCATVLGVIAPKEWTLIADKILISKMSNGVTREHDSYTDQNIKQADANLLAYPLKLITDKEQIERDLKYYQTKIPQSD
TPAMTQAIFSLLYSRLEDSDQAYHWFKDAYQPNLNPPFRVISECKGGTNPYFSTGAGGVLQAVIMGFGGLDIDAAGGIKQ
VKSVLPKNWKKLTITGIGIEKKTFVLTH

Specific function: Hydrolyzes kojibiose in the presence of an inorganic phosphoric acid to form D-glucose and beta-D-glucose-1-phosphoric acid. Can act with alpha-1,2-oligoglucans, such as selaginose, as substrate, but more slowly. Inactive when dissaccharides with linkages

COG id: COG1554

COG function: function code G; Trehalose and maltose hydrolases (possible phosphorylases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 65 family [H]

Homologues:

Organism=Homo sapiens, GI187829418, Length=418, Percent_Identity=34.688995215311, Blast_Score=228, Evalue=2e-59,
Organism=Escherichia coli, GI1787575, Length=698, Percent_Identity=25.9312320916905, Blast_Score=191, Evalue=9e-50,
Organism=Saccharomyces cerevisiae, GI6325283, Length=426, Percent_Identity=32.1596244131455, Blast_Score=186, Evalue=1e-47,
Organism=Drosophila melanogaster, GI24583760, Length=389, Percent_Identity=34.4473007712082, Blast_Score=194, Evalue=2e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008928
- InterPro:   IPR012341
- InterPro:   IPR011013
- InterPro:   IPR005194
- InterPro:   IPR005195
- InterPro:   IPR005196
- InterPro:   IPR017045 [H]

Pfam domain/function: PF03633 Glyco_hydro_65C; PF03632 Glyco_hydro_65m; PF03636 Glyco_hydro_65N [H]

EC number: =2.4.1.230 [H]

Molecular weight: Translated: 75370; Mature: 75370

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLCGSNYLYSQDPWKLSADKPDSNNYYGETVANGMIGIISSPEPLKVKEVVLAGTYDIYK
CCCCCCCCCCCCCCEECCCCCCCCCCCCHHHHCCEEEEECCCCCCEEEHEEEECCHHHHH
RGRVSSFIPNYNLLNMKLAFNGESVQTYNINNYKQELDMRNGAFTGSFQFKDLATVTYSY
CCCHHHHCCCCCEEEEEEEECCCEEEEEECCHHHHHHHCCCCCEECCEECCHHHHHHHHH
YALRHLPHCIMMVVNINTQKDTEINVENLLETPSSLNNQQNYFQNITNTHVNIPLLTSVA
HHHHHCCCEEEEEEECCCCCCCCCCHHHHHCCCHHCCCHHHHHHHHCCCEECCEEEEEEE
FTPTGRSKIAVSNTFLFDEGKKLQPEILHRMNDADMHAMSFDKKIKAGKTYSFALIGSLI
ECCCCCCEEEEEEEEEECCCCCCCHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHH
SSDHINDPYNEAERLTIYAALEGKSRLLNRHMQEWNSLWQSDIQVEGDPQAQQDIRSMLY
HCCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHH
HLYSFTRKSTSLSPSPMGLSGLGYNGHVFWDTEIWMFPPMLLLHPEIAKSMIEYRYQRLD
HHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCHHHCCCHHEECHHHHHHHHHHHHHHHH
AARKKAAIYGYDGAMFPWESADSGAEETPVNALTGAFEHHVTGDVAIAAWQYYLVTGDKE
HHHHCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEEEEEEECCHH
WLKEKGWPILKATAEFWASRVEKNDKGEYEIKNVVAADEWAENIDNNAYTNGTAIRNLQY
HHHHCCCCEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
ASKCATVLGVIAPKEWTLIADKILISKMSNGVTREHDSYTDQNIKQADANLLAYPLKLIT
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHC
DKEQIERDLKYYQTKIPQSDTPAMTQAIFSLLYSRLEDSDQAYHWFKDAYQPNLNPPFRV
CHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHH
ISECKGGTNPYFSTGAGGVLQAVIMGFGGLDIDAAGGIKQVKSVLPKNWKKLTITGIGIE
HHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCEEEEEEECCC
KKTFVLTH
CCEEEEEC
>Mature Secondary Structure
MLCGSNYLYSQDPWKLSADKPDSNNYYGETVANGMIGIISSPEPLKVKEVVLAGTYDIYK
CCCCCCCCCCCCCCEECCCCCCCCCCCCHHHHCCEEEEECCCCCCEEEHEEEECCHHHHH
RGRVSSFIPNYNLLNMKLAFNGESVQTYNINNYKQELDMRNGAFTGSFQFKDLATVTYSY
CCCHHHHCCCCCEEEEEEEECCCEEEEEECCHHHHHHHCCCCCEECCEECCHHHHHHHHH
YALRHLPHCIMMVVNINTQKDTEINVENLLETPSSLNNQQNYFQNITNTHVNIPLLTSVA
HHHHHCCCEEEEEEECCCCCCCCCCHHHHHCCCHHCCCHHHHHHHHCCCEECCEEEEEEE
FTPTGRSKIAVSNTFLFDEGKKLQPEILHRMNDADMHAMSFDKKIKAGKTYSFALIGSLI
ECCCCCCEEEEEEEEEECCCCCCCHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHH
SSDHINDPYNEAERLTIYAALEGKSRLLNRHMQEWNSLWQSDIQVEGDPQAQQDIRSMLY
HCCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHH
HLYSFTRKSTSLSPSPMGLSGLGYNGHVFWDTEIWMFPPMLLLHPEIAKSMIEYRYQRLD
HHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCHHHCCCHHEECHHHHHHHHHHHHHHHH
AARKKAAIYGYDGAMFPWESADSGAEETPVNALTGAFEHHVTGDVAIAAWQYYLVTGDKE
HHHHCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEEEEEEECCHH
WLKEKGWPILKATAEFWASRVEKNDKGEYEIKNVVAADEWAENIDNNAYTNGTAIRNLQY
HHHHCCCCEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
ASKCATVLGVIAPKEWTLIADKILISKMSNGVTREHDSYTDQNIKQADANLLAYPLKLIT
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHC
DKEQIERDLKYYQTKIPQSDTPAMTQAIFSLLYSRLEDSDQAYHWFKDAYQPNLNPPFRV
CHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHH
ISECKGGTNPYFSTGAGGVLQAVIMGFGGLDIDAAGGIKQVKSVLPKNWKKLTITGIGIE
HHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCEEEEEEECCC
KKTFVLTH
CCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA