| Definition | Flavobacterium johnsoniae UW101 chromosome, complete genome. |
|---|---|
| Accession | NC_009441 |
| Length | 6,096,872 |
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The map label for this gene is pemA [H]
Identifier: 146301984
GI number: 146301984
Start: 5126741
End: 5128741
Strand: Reverse
Name: pemA [H]
Synonym: Fjoh_4248
Alternate gene names: 146301984
Gene position: 5128741-5126741 (Counterclockwise)
Preceding gene: 146301985
Following gene: 146301983
Centisome position: 84.12
GC content: 34.83
Gene sequence:
>2001_bases ATGATTCAGTTAAAAAAAATCATATTACTTTTTAGTATTGCTTTGAGTTTTAGCATCCAGGCACAGAATACATATAAAAA CTGGTCGGATATAATTCGTAAAAGTGATGCATCATGGTTTGGCAGTGATGAAGCTAAAAAGATTGCCGAAAATGTACTGC TTTATCAAAGAGATATTGGCGGATGGCCAAAAAACATTCAAATGCAGAATGAACTGACTCCTGCGCAAAAAAAGGATCTG CTTGAAATTAAAAAGACCACAAAGGAAACCACAACAGACAACGGAGCAACGACTCAGGAAATGCTCTTTATGTCTAAAAT GTATGCTCAGGTAAAAGACGAGCGATATAAAGAATCATTTTTAAAAGGATTAAATTATTTGCTCGAAGCACAATATGCAA ATGGAGGCTGGCCTCAGTTTTATCCATTAAAAAAAGGATATTATACACACATTACTTATAATGATGATTCGATGATCAAT ATAATGAATGTGATAAAAGCTGTTGCAGACGAAACTGATTATTACAGTATAAAACCTTCAAAAGATATTGTAGAAAAGAG TAAAAAAGCTTTTGACAAAGGAATTGACTGTATTTTAAAAACACAATACAAGCAAAATGGTATTTTAACAGCGTGGTGTG CGCAGCATGATGAAGTTACTTTTGCCCCGGCAAATGCAAGAGCTTTTGAACTGGCGTCCTTAAGCGGTTACGAATCAACA AAAATTGTTTTGCTGTTAATGGCTGTCGAAAAACCTTCACGAGAAATAGTCATCGCTGTAAAAAGTGCTGTTGAATGGTT CGAAAAAACTAAAATTACCAATTTAGAAGAAAAAAGAGTTTTAAACGATGCAGGAAAAGTTGTAGACAAAAAAATGATTC CTGCTGTAAATGCGGGACCAATTTGGGCTCGGTTTATGGATTTAGAAACTAACGAACCTTTTTTCTGTGATCGTGACGGC ATTAAAAAGAAGTCAATAGATCAAATTGGCGCCGAAAGACGAAACGGTTATTCATGGTATACAGACGCGCCAAAAGAAGT CTTAAAGAAATTTCCAGACTGGGCAGTTAAAAATGGTACAAAAGTTTCAGCTTCTGAAAAGAAAAATGTCAGTCTAATTA CAGTTGCTCAGGATGGATCAGGAGATTTTACTAAAATACAAGATGCAGTGTATGCCTGTCCGGCATTTCCTTATGAGAAA GTCACAATTTATGTGAAAAACGGAGTTTACAATGAAAAAGTCCGAATTCCGGAATGGAATAATAATGTGATTTTAAAAGG CGAAAGCAAAGAGAATACTATCATTACTTTTGATGATAATTTTTCAAAAATCAATTTAGGACGAAACAGTACTTTTTATA CATCAACGCTTTTAGTAGAAGGAGATGATTTTTCAGCATCTAACTTAACTCTCAAAAATGCTTCAGGCGATAAAGGACAA GCAATAGCCTTATCTGTTACCGGAACTCGCGCTAAAATTTCCAACTGTACTATTTTAGGAAATCAGGATACCTTATACTT ATCAGGAAAAAATGCAAAGCAGTACTTTAAAGACTGCTATATAGAAGGTACAACCGATTTTATCTTTGGAGGTGCTACAG CCTTATTTGAAAATTGTATCATTCACAGTATTAAAAGTTCGTATGTTACAGCAGCTTCAACTCCTGAAGGAGTAGATTTC GGTTTTGTTTTTAAGAACTGTAAACTAACCGCTGAAACTGCAGCAAATGCAGTCTATCTAGGACGACCATGGCGCATTTA TGCTAAAACAGCTTTTATAAACTGCGAATTAGGTAAACAAATTAAACCTGAAGGCTGGGAAAACTGGTCAAAACCAGATG CAGAGAAAAATACCTTTTATGCCGAATATAATAACAGCGGAGAAGGATTTCAGCCAAAGAAAAGAGTAACATGGTCACAT CAGCTTACCAAAAAGGAAGCCGATAAATATTCAATAGAAAATATCTTAAAAGATACAGTGGCAAATTGGTATTCTAATTA A
Upstream 100 bases:
>100_bases TGGTGCTAAAATGGGACTTTTTGCTTTAAGCGAAAAAGTAACAAACGATTCTGGAAATGTGGCCGTTGATTGGTTTAGGG TAACGAAATAAAATTTTAAA
Downstream 100 bases:
>100_bases GGTGCTTAGGTTCTAATATGCTAAGTTACTAAGTATCTGAATTAGACTTAAAAACTTAATATCTTAGAACATCTGTACCT AAAACCTTAGAACCTTAGCA
Product: pectate lyase
Products: NA
Alternate protein names: PE A; Pectin methylesterase A [H]
Number of amino acids: Translated: 666; Mature: 666
Protein sequence:
>666_residues MIQLKKIILLFSIALSFSIQAQNTYKNWSDIIRKSDASWFGSDEAKKIAENVLLYQRDIGGWPKNIQMQNELTPAQKKDL LEIKKTTKETTTDNGATTQEMLFMSKMYAQVKDERYKESFLKGLNYLLEAQYANGGWPQFYPLKKGYYTHITYNDDSMIN IMNVIKAVADETDYYSIKPSKDIVEKSKKAFDKGIDCILKTQYKQNGILTAWCAQHDEVTFAPANARAFELASLSGYEST KIVLLLMAVEKPSREIVIAVKSAVEWFEKTKITNLEEKRVLNDAGKVVDKKMIPAVNAGPIWARFMDLETNEPFFCDRDG IKKKSIDQIGAERRNGYSWYTDAPKEVLKKFPDWAVKNGTKVSASEKKNVSLITVAQDGSGDFTKIQDAVYACPAFPYEK VTIYVKNGVYNEKVRIPEWNNNVILKGESKENTIITFDDNFSKINLGRNSTFYTSTLLVEGDDFSASNLTLKNASGDKGQ AIALSVTGTRAKISNCTILGNQDTLYLSGKNAKQYFKDCYIEGTTDFIFGGATALFENCIIHSIKSSYVTAASTPEGVDF GFVFKNCKLTAETAANAVYLGRPWRIYAKTAFINCELGKQIKPEGWENWSKPDAEKNTFYAEYNNSGEGFQPKKRVTWSH QLTKKEADKYSIENILKDTVANWYSN
Sequences:
>Translated_666_residues MIQLKKIILLFSIALSFSIQAQNTYKNWSDIIRKSDASWFGSDEAKKIAENVLLYQRDIGGWPKNIQMQNELTPAQKKDL LEIKKTTKETTTDNGATTQEMLFMSKMYAQVKDERYKESFLKGLNYLLEAQYANGGWPQFYPLKKGYYTHITYNDDSMIN IMNVIKAVADETDYYSIKPSKDIVEKSKKAFDKGIDCILKTQYKQNGILTAWCAQHDEVTFAPANARAFELASLSGYEST KIVLLLMAVEKPSREIVIAVKSAVEWFEKTKITNLEEKRVLNDAGKVVDKKMIPAVNAGPIWARFMDLETNEPFFCDRDG IKKKSIDQIGAERRNGYSWYTDAPKEVLKKFPDWAVKNGTKVSASEKKNVSLITVAQDGSGDFTKIQDAVYACPAFPYEK VTIYVKNGVYNEKVRIPEWNNNVILKGESKENTIITFDDNFSKINLGRNSTFYTSTLLVEGDDFSASNLTLKNASGDKGQ AIALSVTGTRAKISNCTILGNQDTLYLSGKNAKQYFKDCYIEGTTDFIFGGATALFENCIIHSIKSSYVTAASTPEGVDF GFVFKNCKLTAETAANAVYLGRPWRIYAKTAFINCELGKQIKPEGWENWSKPDAEKNTFYAEYNNSGEGFQPKKRVTWSH QLTKKEADKYSIENILKDTVANWYSN >Mature_666_residues MIQLKKIILLFSIALSFSIQAQNTYKNWSDIIRKSDASWFGSDEAKKIAENVLLYQRDIGGWPKNIQMQNELTPAQKKDL LEIKKTTKETTTDNGATTQEMLFMSKMYAQVKDERYKESFLKGLNYLLEAQYANGGWPQFYPLKKGYYTHITYNDDSMIN IMNVIKAVADETDYYSIKPSKDIVEKSKKAFDKGIDCILKTQYKQNGILTAWCAQHDEVTFAPANARAFELASLSGYEST KIVLLLMAVEKPSREIVIAVKSAVEWFEKTKITNLEEKRVLNDAGKVVDKKMIPAVNAGPIWARFMDLETNEPFFCDRDG IKKKSIDQIGAERRNGYSWYTDAPKEVLKKFPDWAVKNGTKVSASEKKNVSLITVAQDGSGDFTKIQDAVYACPAFPYEK VTIYVKNGVYNEKVRIPEWNNNVILKGESKENTIITFDDNFSKINLGRNSTFYTSTLLVEGDDFSASNLTLKNASGDKGQ AIALSVTGTRAKISNCTILGNQDTLYLSGKNAKQYFKDCYIEGTTDFIFGGATALFENCIIHSIKSSYVTAASTPEGVDF GFVFKNCKLTAETAANAVYLGRPWRIYAKTAFINCELGKQIKPEGWENWSKPDAEKNTFYAEYNNSGEGFQPKKRVTWSH QLTKKEADKYSIENILKDTVANWYSN
Specific function: Involved in maceration and soft-rotting of plant tissue [H]
COG id: COG4677
COG function: function code G; Pectin methylesterase
Gene ontology:
Cell location: Secreted [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the pectinesterase family [H]
Homologues:
None
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR012334 - InterPro: IPR011050 - InterPro: IPR018040 - InterPro: IPR000070 [H]
Pfam domain/function: PF01095 Pectinesterase [H]
EC number: =3.1.1.11 [H]
Molecular weight: Translated: 75246; Mature: 75246
Theoretical pI: Translated: 8.72; Mature: 8.72
Prosite motif: PS00012 PHOSPHOPANTETHEINE ; PS00800 PECTINESTERASE_1 ; PS00503 PECTINESTERASE_2
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.4 %Cys (Translated Protein) 1.5 %Met (Translated Protein) 2.9 %Cys+Met (Translated Protein) 1.4 %Cys (Mature Protein) 1.5 %Met (Mature Protein) 2.9 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MIQLKKIILLFSIALSFSIQAQNTYKNWSDIIRKSDASWFGSDEAKKIAENVLLYQRDIG CCHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHEEECCCC GWPKNIQMQNELTPAQKKDLLEIKKTTKETTTDNGATTQEMLFMSKMYAQVKDERYKESF CCCCCCEECCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH LKGLNYLLEAQYANGGWPQFYPLKKGYYTHITYNDDSMINIMNVIKAVADETDYYSIKPS HHHHHHHEEEEECCCCCCEEEECCCCEEEEEEECCCCHHHHHHHHHHHHCCCCEEEECCC KDIVEKSKKAFDKGIDCILKTQYKQNGILTAWCAQHDEVTFAPANARAFELASLSGYEST HHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEECCCCCEEEECCCCCEEEEEECCCCCCC KIVLLLMAVEKPSREIVIAVKSAVEWFEKTKITNLEEKRVLNDAGKVVDKKMIPAVNAGP EEEEEEEECCCCCCEEEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC IWARFMDLETNEPFFCDRDGIKKKSIDQIGAERRNGYSWYTDAPKEVLKKFPDWAVKNGT CEEEEEECCCCCCEEECCCCCCHHHHHHHHHHHCCCCCEECCCHHHHHHHCCCCHHCCCC KVSASEKKNVSLITVAQDGSGDFTKIQDAVYACPAFPYEKVTIYVKNGVYNEKVRIPEWN EECCCCCCCEEEEEEEECCCCCHHHHHHHHHCCCCCCCCEEEEEEECCCCCCEEECCCCC NNVILKGESKENTIITFDDNFSKINLGRNSTFYTSTLLVEGDDFSASNLTLKNASGDKGQ CCEEEECCCCCCEEEEECCCCCEEECCCCCEEEEEEEEEECCCCCCCCEEEECCCCCCCC AIALSVTGTRAKISNCTILGNQDTLYLSGKNAKQYFKDCYIEGTTDFIFGGATALFENCI EEEEEEECCCEEECEEEEECCCCEEEEECCCHHHHHHHHEECCCCCEEECCHHHHHHHHH IHSIKSSYVTAASTPEGVDFGFVFKNCKLTAETAANAVYLGRPWRIYAKTAFINCELGKQ HHHHHHCEEEECCCCCCCEEEEEEECCEEEHHHCCCEEEECCCEEEEEEEEEEEEECCCC IKPEGWENWSKPDAEKNTFYAEYNNSGEGFQPKKRVTWSHQLTKKEADKYSIENILKDTV CCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH ANWYSN HHHHCC >Mature Secondary Structure MIQLKKIILLFSIALSFSIQAQNTYKNWSDIIRKSDASWFGSDEAKKIAENVLLYQRDIG CCHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHEEECCCC GWPKNIQMQNELTPAQKKDLLEIKKTTKETTTDNGATTQEMLFMSKMYAQVKDERYKESF CCCCCCEECCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH LKGLNYLLEAQYANGGWPQFYPLKKGYYTHITYNDDSMINIMNVIKAVADETDYYSIKPS HHHHHHHEEEEECCCCCCEEEECCCCEEEEEEECCCCHHHHHHHHHHHHCCCCEEEECCC KDIVEKSKKAFDKGIDCILKTQYKQNGILTAWCAQHDEVTFAPANARAFELASLSGYEST HHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEECCCCCEEEECCCCCEEEEEECCCCCCC KIVLLLMAVEKPSREIVIAVKSAVEWFEKTKITNLEEKRVLNDAGKVVDKKMIPAVNAGP EEEEEEEECCCCCCEEEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC IWARFMDLETNEPFFCDRDGIKKKSIDQIGAERRNGYSWYTDAPKEVLKKFPDWAVKNGT CEEEEEECCCCCCEEECCCCCCHHHHHHHHHHHCCCCCEECCCHHHHHHHCCCCHHCCCC KVSASEKKNVSLITVAQDGSGDFTKIQDAVYACPAFPYEKVTIYVKNGVYNEKVRIPEWN EECCCCCCCEEEEEEEECCCCCHHHHHHHHHCCCCCCCCEEEEEEECCCCCCEEECCCCC NNVILKGESKENTIITFDDNFSKINLGRNSTFYTSTLLVEGDDFSASNLTLKNASGDKGQ CCEEEECCCCCCEEEEECCCCCEEECCCCCEEEEEEEEEECCCCCCCCEEEECCCCCCCC AIALSVTGTRAKISNCTILGNQDTLYLSGKNAKQYFKDCYIEGTTDFIFGGATALFENCI EEEEEEECCCEEECEEEEECCCCEEEEECCCHHHHHHHHEECCCCCEEECCHHHHHHHHH IHSIKSSYVTAASTPEGVDFGFVFKNCKLTAETAANAVYLGRPWRIYAKTAFINCELGKQ HHHHHHCEEEECCCCCCCEEEEEEECCEEEHHHCCCEEEECCCEEEEEEEEEEEEECCCC IKPEGWENWSKPDAEKNTFYAEYNNSGEGFQPKKRVTWSHQLTKKEADKYSIENILKDTV CCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH ANWYSN HHHHCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 8370537 [H]