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The map label for this gene is lacZ [H]

Identifier: 146301933

GI number: 146301933

Start: 5045116

End: 5047527

Strand: Direct

Name: lacZ [H]

Synonym: Fjoh_4197

Alternate gene names: 146301933

Gene position: 5045116-5047527 (Clockwise)

Preceding gene: 146301932

Following gene: 146301934

Centisome position: 82.75

GC content: 36.48

Gene sequence:

>2412_bases
ATGAATAAAACGAATAAAACTTTTTCAATTTTTGCGCTTTTTGTTTTTTTTCAGATTTGTCTGAGCAGCAGTTTTGCTCA
GACAAAGATGAATATTAACGACAACTGGCTTTATTTAGAAAATCATACTCCAAACCTTACCGAAGCGCAAAAAGCTTCTA
ACTGGGTTTCTCTGAACTTACCGCATACCTGGAATGCCGAAGATGCAACCGATTTAGTTCCGGGGTACAGGCGCGATGCA
AGCTGGTATCAAAAAAAATTAAATATCACACAGATCGACAAAAACAAGGTTTATTCCTTATACTTTGAAGGATCAAACGT
AACGACCAAAGTTTATGTAAACGGTAAAGAAGCCGGATCACATATTGGCGGATATATTGGTTTTTCAATCGATATTACAA
ATTTGATTAAGGAAGGAAATAATGAGATTTTTGTTCGTGTGGACAATAGTTATGATATCGAAATTATCCCGTCTCAAAAA
AGTGATTTCTTTATTTACGGCGGTATCACGCGCGATGTCTGGCTGGTTTCAAAATACAAAAATCATATCGAGAATATAAA
GATTACAACTCCGGAAGTTTCTGCTAAAAAAGCTTCGGTGCAAATTGTTTCTTCTTTTGTAAATCCAGATAATTCAAAAG
ACTTATCATTGACGGTTACTTTAAAAAATCCGAAAGGCAAAAAAATAGCAAGCAAAACAATTCCTGTTACTGATAAAAAT
TCGACCATCACTTTTGAAAATATCAAAAACCCAGAACTTTGGGATACAGAAAATCCGAATTTATACAAACTGACCGCTGT
TTTATCTGAAAAAAATCAAATTAAAGACAGCGTTGCGGAAAAAGTAGGTTTTAGATGGTTTGAATTTAAAGATCATGGTC
CATTTTACCTTAACGGAAAACGTCTGCTTATTCGCGGTACACACCGTCATGAAGAACAAGCCGGAGTTGGCGCTGCCATG
AGCAACGAACAGCATTGGGCAGATATGAAATCAATAAAAGAAATGGGTGCAAATTTTGTCCGTTTAGCCCATTATCCGCA
AGATCCCGAAGTTTATAAAGCCTGCGACGAGTTAGGTTTATTGGTTTGGGATGAACTTCCCTGGTGTCGCGGCGGTATTG
GTAATGAGGTTTGGAAAACCAATACTAAAAATATGCTGGCCGAGATTATCGACCAAAATTACAATCATCCAAGTATTATT
ATCTGGTCTTTAGGAAACGAAATGAACTGGCTTCCTGATTTTCCTGATGGTGATAATCCAGAAAAAACAAATGTGTTTTT
AACTGAATTAAATGACATCGCTCATAAACTCGATCCTACAAGAAAAACGGCTATTAGAAAATATTATGAAGGTTCTCATA
TTGTAGATGTTTTCTCGCCGTCAATCTGGTCAGGATGGTATTCTGGAAGTTACAAAAGTTATCAAAAAGCAATTAACGTT
TATAAAAAAGAATACAAGCATTTTATTCATGCTGAATATGGCGGAGACAGCCATGTAGGCCGTCACAGTGAAAATCCGGT
TACGGGTGAAAATGTGATTAAAGCTGAAGGCTGGGAAGAAGCGATTGTTCAGACTAAAGTTGCAAACATTGCTCAAATTG
GCGATTGGAGCGAAAATTATATAGTCGATTTATTTGACTGGCATTTGCATATATCCGAGAATGACCCGGCGTTTGTGGGT
AATGTACAATGGGCATTTAAGGATTTTGCAACGCCGTTACGTCCGGAAGATGATATTCCGTACATGAATCAAAAAGGACT
TACTGATCGAAACGGAATTCCGAAAGATGCGTATTATGTTTTTAAAAGTTATTGGGCAAAAGAACCTTTCACTTATATTG
AATCGCATACCTGGACAGAGCGTCAGGGACCTGAAAATACAGCGAGAACGATCAGTGTTTTCAGTAACTGTGATAAAGTA
ACTTTATCTCATAACGGAAAATCATTAGGAGAAAAACAGCGAAATCTTTCTCTCTATCCGGCAAATGGCTTAACTTGGGA
TGTCAATTTTACAAAAGGTGAAAATGTTCTAATTGCTGTTGGAAAAACGAAAGAAGGAAAAACGGTTTCTGATACGCTGA
AAGTTAATTATCGTTTTAATAAAAATGATACGGCGGTTTCATTACAATTATCATCAGAAAAGCTGAAAAACGGCAATTAT
CTGGTAACCGCAATTGCAGTTGACAATAATAATTTGCGCTGTCTGGATTATGAAGCGAGTGTTTATTTTCAATGTTTAAA
GGGAGGAAAAACACTAAAAAATCAAGGAACACCAACCGGAAGCGAATCCATAAAAATGGCAAATGGAAAAGCTTCTATAG
AAGTTATTCCTGATGGTTCTAATAATCCAATCGAAATGACGGCTTTGAACCAAAGCTTTAAAGGAGAATATTTGAAGATT
CCAGTTGAGTAA

Upstream 100 bases:

>100_bases
GTGCAAAGGCTCAAAGGTTCAAAGTAACAAAGGTTTTTAATCTTTGAACCTTTGAGCCAGAAAAACCTTTGAACCTTTGT
AACTATGAACCTTTGTAACT

Downstream 100 bases:

>100_bases
AAAAGGTTCTAAGCTGCTAAGGGACTAAGGTGCTAAGTTTTATGTTTCACGCAGATTTTGCAGATTGAGCAGATTTAATT
TTGATCTGCTTAAATCTTTT

Product: glycoside hydrolase family protein

Products: NA

Alternate protein names: Beta-gal; Lactase [H]

Number of amino acids: Translated: 803; Mature: 803

Protein sequence:

>803_residues
MNKTNKTFSIFALFVFFQICLSSSFAQTKMNINDNWLYLENHTPNLTEAQKASNWVSLNLPHTWNAEDATDLVPGYRRDA
SWYQKKLNITQIDKNKVYSLYFEGSNVTTKVYVNGKEAGSHIGGYIGFSIDITNLIKEGNNEIFVRVDNSYDIEIIPSQK
SDFFIYGGITRDVWLVSKYKNHIENIKITTPEVSAKKASVQIVSSFVNPDNSKDLSLTVTLKNPKGKKIASKTIPVTDKN
STITFENIKNPELWDTENPNLYKLTAVLSEKNQIKDSVAEKVGFRWFEFKDHGPFYLNGKRLLIRGTHRHEEQAGVGAAM
SNEQHWADMKSIKEMGANFVRLAHYPQDPEVYKACDELGLLVWDELPWCRGGIGNEVWKTNTKNMLAEIIDQNYNHPSII
IWSLGNEMNWLPDFPDGDNPEKTNVFLTELNDIAHKLDPTRKTAIRKYYEGSHIVDVFSPSIWSGWYSGSYKSYQKAINV
YKKEYKHFIHAEYGGDSHVGRHSENPVTGENVIKAEGWEEAIVQTKVANIAQIGDWSENYIVDLFDWHLHISENDPAFVG
NVQWAFKDFATPLRPEDDIPYMNQKGLTDRNGIPKDAYYVFKSYWAKEPFTYIESHTWTERQGPENTARTISVFSNCDKV
TLSHNGKSLGEKQRNLSLYPANGLTWDVNFTKGENVLIAVGKTKEGKTVSDTLKVNYRFNKNDTAVSLQLSSEKLKNGNY
LVTAIAVDNNNLRCLDYEASVYFQCLKGGKTLKNQGTPTGSESIKMANGKASIEVIPDGSNNPIEMTALNQSFKGEYLKI
PVE

Sequences:

>Translated_803_residues
MNKTNKTFSIFALFVFFQICLSSSFAQTKMNINDNWLYLENHTPNLTEAQKASNWVSLNLPHTWNAEDATDLVPGYRRDA
SWYQKKLNITQIDKNKVYSLYFEGSNVTTKVYVNGKEAGSHIGGYIGFSIDITNLIKEGNNEIFVRVDNSYDIEIIPSQK
SDFFIYGGITRDVWLVSKYKNHIENIKITTPEVSAKKASVQIVSSFVNPDNSKDLSLTVTLKNPKGKKIASKTIPVTDKN
STITFENIKNPELWDTENPNLYKLTAVLSEKNQIKDSVAEKVGFRWFEFKDHGPFYLNGKRLLIRGTHRHEEQAGVGAAM
SNEQHWADMKSIKEMGANFVRLAHYPQDPEVYKACDELGLLVWDELPWCRGGIGNEVWKTNTKNMLAEIIDQNYNHPSII
IWSLGNEMNWLPDFPDGDNPEKTNVFLTELNDIAHKLDPTRKTAIRKYYEGSHIVDVFSPSIWSGWYSGSYKSYQKAINV
YKKEYKHFIHAEYGGDSHVGRHSENPVTGENVIKAEGWEEAIVQTKVANIAQIGDWSENYIVDLFDWHLHISENDPAFVG
NVQWAFKDFATPLRPEDDIPYMNQKGLTDRNGIPKDAYYVFKSYWAKEPFTYIESHTWTERQGPENTARTISVFSNCDKV
TLSHNGKSLGEKQRNLSLYPANGLTWDVNFTKGENVLIAVGKTKEGKTVSDTLKVNYRFNKNDTAVSLQLSSEKLKNGNY
LVTAIAVDNNNLRCLDYEASVYFQCLKGGKTLKNQGTPTGSESIKMANGKASIEVIPDGSNNPIEMTALNQSFKGEYLKI
PVE
>Mature_803_residues
MNKTNKTFSIFALFVFFQICLSSSFAQTKMNINDNWLYLENHTPNLTEAQKASNWVSLNLPHTWNAEDATDLVPGYRRDA
SWYQKKLNITQIDKNKVYSLYFEGSNVTTKVYVNGKEAGSHIGGYIGFSIDITNLIKEGNNEIFVRVDNSYDIEIIPSQK
SDFFIYGGITRDVWLVSKYKNHIENIKITTPEVSAKKASVQIVSSFVNPDNSKDLSLTVTLKNPKGKKIASKTIPVTDKN
STITFENIKNPELWDTENPNLYKLTAVLSEKNQIKDSVAEKVGFRWFEFKDHGPFYLNGKRLLIRGTHRHEEQAGVGAAM
SNEQHWADMKSIKEMGANFVRLAHYPQDPEVYKACDELGLLVWDELPWCRGGIGNEVWKTNTKNMLAEIIDQNYNHPSII
IWSLGNEMNWLPDFPDGDNPEKTNVFLTELNDIAHKLDPTRKTAIRKYYEGSHIVDVFSPSIWSGWYSGSYKSYQKAINV
YKKEYKHFIHAEYGGDSHVGRHSENPVTGENVIKAEGWEEAIVQTKVANIAQIGDWSENYIVDLFDWHLHISENDPAFVG
NVQWAFKDFATPLRPEDDIPYMNQKGLTDRNGIPKDAYYVFKSYWAKEPFTYIESHTWTERQGPENTARTISVFSNCDKV
TLSHNGKSLGEKQRNLSLYPANGLTWDVNFTKGENVLIAVGKTKEGKTVSDTLKVNYRFNKNDTAVSLQLSSEKLKNGNY
LVTAIAVDNNNLRCLDYEASVYFQCLKGGKTLKNQGTPTGSESIKMANGKASIEVIPDGSNNPIEMTALNQSFKGEYLKI
PVE

Specific function: The Wild-Type Enzyme Is An Ineffective Lactase. Two Classes Of Point Mutations Dramatically Improve Activity Of The Enzyme. [C]

COG id: COG3250

COG function: function code G; Beta-galactosidase/beta-glucuronidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 2 family [H]

Homologues:

Organism=Homo sapiens, GI268834192, Length=576, Percent_Identity=24.1319444444444, Blast_Score=129, Evalue=8e-30,
Organism=Escherichia coli, GI48994920, Length=396, Percent_Identity=27.7777777777778, Blast_Score=140, Evalue=3e-34,
Organism=Escherichia coli, GI1787903, Length=609, Percent_Identity=26.1083743842365, Blast_Score=137, Evalue=3e-33,
Organism=Escherichia coli, GI1786539, Length=330, Percent_Identity=29.6969696969697, Blast_Score=111, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17510825, Length=598, Percent_Identity=25.5852842809365, Blast_Score=133, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24651745, Length=602, Percent_Identity=25.7475083056478, Blast_Score=134, Evalue=4e-31,
Organism=Drosophila melanogaster, GI62471735, Length=612, Percent_Identity=24.8366013071895, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI19922582, Length=612, Percent_Identity=24.8366013071895, Blast_Score=124, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24655438, Length=612, Percent_Identity=24.8366013071895, Blast_Score=124, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003344
- InterPro:   IPR008979
- InterPro:   IPR006101
- InterPro:   IPR013812
- InterPro:   IPR006104
- InterPro:   IPR006102
- InterPro:   IPR006103
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00703 Glyco_hydro_2; PF02836 Glyco_hydro_2_C; PF02837 Glyco_hydro_2_N [H]

EC number: =3.2.1.23 [H]

Molecular weight: Translated: 91172; Mature: 91172

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKTNKTFSIFALFVFFQICLSSSFAQTKMNINDNWLYLENHTPNLTEAQKASNWVSLNL
CCCCCCHHHHHHHHHHHHHHHCCCHHEEEECCCCCEEEEECCCCCCCHHHHCCCEEEEEC
PHTWNAEDATDLVPGYRRDASWYQKKLNITQIDKNKVYSLYFEGSNVTTKVYVNGKEAGS
CCCCCCCCCHHCCCCCCCCHHHHHHCCCEEEECCCCEEEEEEECCCEEEEEEECCHHCCC
HIGGYIGFSIDITNLIKEGNNEIFVRVDNSYDIEIIPSQKSDFFIYGGITRDVWLVSKYK
CCCEEEEEEEEHHHHHHCCCCEEEEEECCCCEEEEEECCCCCEEEECCCCHHHHHHHHHH
NHIENIKITTPEVSAKKASVQIVSSFVNPDNSKDLSLTVTLKNPKGKKIASKTIPVTDKN
HHHCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCEEECCCCCCCCCC
STITFENIKNPELWDTENPNLYKLTAVLSEKNQIKDSVAEKVGFRWFEFKDHGPFYLNGK
CEEEEECCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCEEEEEECCCCEEECCC
RLLIRGTHRHEEQAGVGAAMSNEQHWADMKSIKEMGANFVRLAHYPQDPEVYKACDELGL
EEEEECCCCCCHHCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHCCE
LVWDELPWCRGGIGNEVWKTNTKNMLAEIIDQNYNHPSIIIWSLGNEMNWLPDFPDGDNP
EEECCCCCCCCCCCCCHHCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCC
EKTNVFLTELNDIAHKLDPTRKTAIRKYYEGSHIVDVFSPSIWSGWYSGSYKSYQKAINV
CCCEEEEEEHHHHHHHCCCHHHHHHHHHCCCCEEEEECCCCHHCCCCCCCHHHHHHHHHH
YKKEYKHFIHAEYGGDSHVGRHSENPVTGENVIKAEGWEEAIVQTKVANIAQIGDWSENY
HHHHHHHEEEEECCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHCCCCCCCE
IVDLFDWHLHISENDPAFVGNVQWAFKDFATPLRPEDDIPYMNQKGLTDRNGIPKDAYYV
EEEEEEEEEEECCCCCEEEECEEEHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
FKSYWAKEPFTYIESHTWTERQGPENTARTISVFSNCDKVTLSHNGKSLGEKQRNLSLYP
HHHHHCCCCCHHHHCCCCCCCCCCCCHHHHHHHHCCCCEEEEECCCCHHCHHHHCEEEEE
ANGLTWDVNFTKGENVLIAVGKTKEGKTVSDTLKVNYRFNKNDTAVSLQLSSEKLKNGNY
CCCCEEEEEECCCCEEEEEEECCCCCCEEEEEEEEEEEECCCCEEEEEEECHHHCCCCCE
LVTAIAVDNNNLRCLDYEASVYFQCLKGGKTLKNQGTPTGSESIKMANGKASIEVIPDGS
EEEEEEECCCCEEEEEECHHHHHHHHCCCCEECCCCCCCCCCCEEEECCCEEEEEEECCC
NNPIEMTALNQSFKGEYLKIPVE
CCCEEEEEECCCCCCCEEEEECC
>Mature Secondary Structure
MNKTNKTFSIFALFVFFQICLSSSFAQTKMNINDNWLYLENHTPNLTEAQKASNWVSLNL
CCCCCCHHHHHHHHHHHHHHHCCCHHEEEECCCCCEEEEECCCCCCCHHHHCCCEEEEEC
PHTWNAEDATDLVPGYRRDASWYQKKLNITQIDKNKVYSLYFEGSNVTTKVYVNGKEAGS
CCCCCCCCCHHCCCCCCCCHHHHHHCCCEEEECCCCEEEEEEECCCEEEEEEECCHHCCC
HIGGYIGFSIDITNLIKEGNNEIFVRVDNSYDIEIIPSQKSDFFIYGGITRDVWLVSKYK
CCCEEEEEEEEHHHHHHCCCCEEEEEECCCCEEEEEECCCCCEEEECCCCHHHHHHHHHH
NHIENIKITTPEVSAKKASVQIVSSFVNPDNSKDLSLTVTLKNPKGKKIASKTIPVTDKN
HHHCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCEEECCCCCCCCCC
STITFENIKNPELWDTENPNLYKLTAVLSEKNQIKDSVAEKVGFRWFEFKDHGPFYLNGK
CEEEEECCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCEEEEEECCCCEEECCC
RLLIRGTHRHEEQAGVGAAMSNEQHWADMKSIKEMGANFVRLAHYPQDPEVYKACDELGL
EEEEECCCCCCHHCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHCCE
LVWDELPWCRGGIGNEVWKTNTKNMLAEIIDQNYNHPSIIIWSLGNEMNWLPDFPDGDNP
EEECCCCCCCCCCCCCHHCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCC
EKTNVFLTELNDIAHKLDPTRKTAIRKYYEGSHIVDVFSPSIWSGWYSGSYKSYQKAINV
CCCEEEEEEHHHHHHHCCCHHHHHHHHHCCCCEEEEECCCCHHCCCCCCCHHHHHHHHHH
YKKEYKHFIHAEYGGDSHVGRHSENPVTGENVIKAEGWEEAIVQTKVANIAQIGDWSENY
HHHHHHHEEEEECCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHCCCCCCCE
IVDLFDWHLHISENDPAFVGNVQWAFKDFATPLRPEDDIPYMNQKGLTDRNGIPKDAYYV
EEEEEEEEEEECCCCCEEEECEEEHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
FKSYWAKEPFTYIESHTWTERQGPENTARTISVFSNCDKVTLSHNGKSLGEKQRNLSLYP
HHHHHCCCCCHHHHCCCCCCCCCCCCHHHHHHHHCCCCEEEEECCCCHHCHHHHCEEEEE
ANGLTWDVNFTKGENVLIAVGKTKEGKTVSDTLKVNYRFNKNDTAVSLQLSSEKLKNGNY
CCCCEEEEEECCCCEEEEEEECCCCCCEEEEEEEEEEEECCCCEEEEEEECHHHCCCCCE
LVTAIAVDNNNLRCLDYEASVYFQCLKGGKTLKNQGTPTGSESIKMANGKASIEVIPDGS
EEEEEEECCCCEEEEEECHHHHHHHHCCCCEECCCCCCCCCCCEEEECCCEEEEEEECCC
NNPIEMTALNQSFKGEYLKIPVE
CCCEEEEEECCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA