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The map label for this gene is pme [H]

Identifier: 146301832

GI number: 146301832

Start: 4911589

End: 4912605

Strand: Reverse

Name: pme [H]

Synonym: Fjoh_4096

Alternate gene names: 146301832

Gene position: 4912605-4911589 (Counterclockwise)

Preceding gene: 146301833

Following gene: 146301831

Centisome position: 80.58

GC content: 34.91

Gene sequence:

>1017_bases
ATGACAAAAAACGAAATAAATAAAAAACATATGAAATACATTCTAACCTTATTTTTAATAACAACACTTTCAATATCAGC
CCAAACGCTTGATAACAAATTGACATTAACCGTTGCTCAAGACGGTTCTGGAGATTTTAAAACGATTCAGGAAGCGGTGA
ATAATGTGAAAGATAATTCGGAGAAACGTGTTGTAATCACTATAAAACCAGGAAAGTATGTTGAGAAATTGGAAATTCCA
GTTTCAAAACCCTTTATTACTTTAAAAGGTCTTGACAGAAATAAAACGATTATTTCATTTGATGATTATTCAGGAAAGCC
GCTTCGTGAACCAGATCCGTCGGGTAAAAAAGAATTTGGAACAGGAACTTCGTATTCTTTCATAATTAAAGGAAATGATT
GTACGCTTGAAAATCTTACTGTCGAAAATACGGCGGGAAGAGTAGGACAGGCGGTTGCACTTCACATTAAAGGCGATAGA
GTAATTGTGAAGAATTGCAATCTTTTAGCAAATCAGGATACCCTTTATTTATCAGAAGGAAACACCCGAACTTATTTTGA
AAACTGTTTCATTAACGGTACAACCGATTTTATCTTTGGCGCTGCAACGGCTTATTTTTATAAATGTACTATTGAAAGTT
TAGTCAATTCCTATATTACCGCGGCTTCAACTCCGCAAGGGCAAGCTTATGGTTTTGTTTTTGTTGATTGCAAGCTTACT
GCAAAAGACAAATCGGTAGACAAAGTATTTTTGGGAAGACCATGGAGACCTTATGCACAAACTGTTTTCATTAATACGGA
TATTGGTTCGCACATTATTCCAGAAGGATGGAATGCCTGGATAGATACTCGTTTTCCGGATAAAGATAAAACAGCTTATT
ACGCGGAATACGGAAGTAAAGGTGCAAGTACAAAAAATATCTCACAGCGAGTGTCATGGTCGTATCAATTAACTAAAGAT
GATATAAAAAAATACAATAAAGATTTAGTTTTGAATGGATGGAATCCGGCCAAATAA

Upstream 100 bases:

>100_bases
CAGTTTGTCATTTCGACGAAGGAGAAATCTTCGCAAGTAGCTCGACAAAGATTTGCGATTAAGATTGCGGAGTTTCTAGT
GTGATTCCTCGTTCCTCGGA

Downstream 100 bases:

>100_bases
ATTTAAAATTGAACTATCCTGCCTGAATTTATGTGTATAAACCACACTAAAAATTAAAATTTCTCTTCAAAAATCTCGTG
AAGGCCTATCATAAAAGGCA

Product: pectinesterase

Products: NA

Alternate protein names: PE; Pectin methylesterase [H]

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MTKNEINKKHMKYILTLFLITTLSISAQTLDNKLTLTVAQDGSGDFKTIQEAVNNVKDNSEKRVVITIKPGKYVEKLEIP
VSKPFITLKGLDRNKTIISFDDYSGKPLREPDPSGKKEFGTGTSYSFIIKGNDCTLENLTVENTAGRVGQAVALHIKGDR
VIVKNCNLLANQDTLYLSEGNTRTYFENCFINGTTDFIFGAATAYFYKCTIESLVNSYITAASTPQGQAYGFVFVDCKLT
AKDKSVDKVFLGRPWRPYAQTVFINTDIGSHIIPEGWNAWIDTRFPDKDKTAYYAEYGSKGASTKNISQRVSWSYQLTKD
DIKKYNKDLVLNGWNPAK

Sequences:

>Translated_338_residues
MTKNEINKKHMKYILTLFLITTLSISAQTLDNKLTLTVAQDGSGDFKTIQEAVNNVKDNSEKRVVITIKPGKYVEKLEIP
VSKPFITLKGLDRNKTIISFDDYSGKPLREPDPSGKKEFGTGTSYSFIIKGNDCTLENLTVENTAGRVGQAVALHIKGDR
VIVKNCNLLANQDTLYLSEGNTRTYFENCFINGTTDFIFGAATAYFYKCTIESLVNSYITAASTPQGQAYGFVFVDCKLT
AKDKSVDKVFLGRPWRPYAQTVFINTDIGSHIIPEGWNAWIDTRFPDKDKTAYYAEYGSKGASTKNISQRVSWSYQLTKD
DIKKYNKDLVLNGWNPAK
>Mature_337_residues
TKNEINKKHMKYILTLFLITTLSISAQTLDNKLTLTVAQDGSGDFKTIQEAVNNVKDNSEKRVVITIKPGKYVEKLEIPV
SKPFITLKGLDRNKTIISFDDYSGKPLREPDPSGKKEFGTGTSYSFIIKGNDCTLENLTVENTAGRVGQAVALHIKGDRV
IVKNCNLLANQDTLYLSEGNTRTYFENCFINGTTDFIFGAATAYFYKCTIESLVNSYITAASTPQGQAYGFVFVDCKLTA
KDKSVDKVFLGRPWRPYAQTVFINTDIGSHIIPEGWNAWIDTRFPDKDKTAYYAEYGSKGASTKNISQRVSWSYQLTKDD
IKKYNKDLVLNGWNPAK

Specific function: Involved in maceration and soft-rotting of plant tissue [H]

COG id: COG4677

COG function: function code G; Pectin methylesterase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pectinesterase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012334
- InterPro:   IPR011050
- InterPro:   IPR018040
- InterPro:   IPR000070 [H]

Pfam domain/function: PF01095 Pectinesterase [H]

EC number: =3.1.1.11 [H]

Molecular weight: Translated: 37873; Mature: 37742

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: PS00503 PECTINESTERASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKNEINKKHMKYILTLFLITTLSISAQTLDNKLTLTVAQDGSGDFKTIQEAVNNVKDNS
CCCCHHHHHHHHHHHHHHHHHHHCCCCEECCCEEEEEEEECCCCCHHHHHHHHHHCCCCC
EKRVVITIKPGKYVEKLEIPVSKPFITLKGLDRNKTIISFDDYSGKPLREPDPSGKKEFG
CCEEEEEECCCCCEEEEECCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCC
TGTSYSFIIKGNDCTLENLTVENTAGRVGQAVALHIKGDRVIVKNCNLLANQDTLYLSEG
CCCCEEEEEECCCCEEEEEEEECCCCCCCCEEEEEEECCEEEEECCCEEECCCEEEEECC
NTRTYFENCFINGTTDFIFGAATAYFYKCTIESLVNSYITAASTPQGQAYGFVFVDCKLT
CCEEEEEEEEECCCHHHHHHHHHHEEEEEEHHHHHHHHHCCCCCCCCCEEEEEEEEEEEE
AKDKSVDKVFLGRPWRPYAQTVFINTDIGSHIIPEGWNAWIDTRFPDKDKTAYYAEYGSK
CCCCCCCEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCEEECCCCCCCCEEEEEECCCC
GASTKNISQRVSWSYQLTKDDIKKYNKDLVLNGWNPAK
CCCCCCHHHHEEEEEEECHHHHHHCCCCEEEECCCCCC
>Mature Secondary Structure 
TKNEINKKHMKYILTLFLITTLSISAQTLDNKLTLTVAQDGSGDFKTIQEAVNNVKDNS
CCCHHHHHHHHHHHHHHHHHHHCCCCEECCCEEEEEEEECCCCCHHHHHHHHHHCCCCC
EKRVVITIKPGKYVEKLEIPVSKPFITLKGLDRNKTIISFDDYSGKPLREPDPSGKKEFG
CCEEEEEECCCCCEEEEECCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCC
TGTSYSFIIKGNDCTLENLTVENTAGRVGQAVALHIKGDRVIVKNCNLLANQDTLYLSEG
CCCCEEEEEECCCCEEEEEEEECCCCCCCCEEEEEEECCEEEEECCCEEECCCEEEEECC
NTRTYFENCFINGTTDFIFGAATAYFYKCTIESLVNSYITAASTPQGQAYGFVFVDCKLT
CCEEEEEEEEECCCHHHHHHHHHHEEEEEEHHHHHHHHHCCCCCCCCCEEEEEEEEEEEE
AKDKSVDKVFLGRPWRPYAQTVFINTDIGSHIIPEGWNAWIDTRFPDKDKTAYYAEYGSK
CCCCCCCEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCEEECCCCCCCCEEEEEECCCC
GASTKNISQRVSWSYQLTKDDIKKYNKDLVLNGWNPAK
CCCCCCHHHHEEEEEEECHHHHHHCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2045776 [H]