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The map label for this gene is nadB [H]

Identifier: 146301792

GI number: 146301792

Start: 4841170

End: 4842693

Strand: Direct

Name: nadB [H]

Synonym: Fjoh_4056

Alternate gene names: 146301792

Gene position: 4841170-4842693 (Clockwise)

Preceding gene: 146301791

Following gene: 146301793

Centisome position: 79.4

GC content: 35.7

Gene sequence:

>1524_bases
ATGCTTAAAACAGATATACTTATAGTAGGTTCTGGTATTTCGGGATTATTTCTTGCCATGAAAACAGCAAAAAAACGTCC
TGATTTATCGATTGTGATTATGACAAAGAAAACGGCAGGAAACACTAATACACAGCTTGCACAAGGCGGAATTGCCGTTG
TAACCAATCATTTACAGGATAGTTTTGAGCAGCATATAAAAGATACACTGAAATCAGGAGGCGGACATTGTGATGAGGAA
ATTGTAAAAATGGTAGTGCAGCAGGCACCGGAAAGGTTAAAAGAATTAATTGAGATTGGGACTTCTTTTGATAAAAATCA
AAACGGTCAATGGGATTTAGGTCTAGAAGGCGGACATTCTCAGCATAGAATTCTGCACCATAAAGACAGATCTGGTTTAG
AAATAGAAAAAAAGCTGCTCCATATTATAACAGAATTGCCTAACATAAAATTATTCGAAAATTATCAGGTTGTCGATTTA
AATACCGAAACAAAAGAAAATGAAACACATTGTACAGGTGCTTTTTTTTATGATAAAAACGAAAACAAAATAAAATATAT
CCGCGCCAGATCGATAATATTAAGCACAGGAGGATGCGGGACTCTTTTTGAAAACACAACAAATCCTGAAATTGCAACCG
GAGACGGAATTGCCATTGCAGCGCGTGCAGGTGTAGAAATTGAAGATATGCAGTACATTCAGTTTCATCCTACGGCTTTG
TACACTGGCAAAAAAAGCCAGCTCTTTTTAATTTCAGAAGCCGTTAGAGGTTTTGGTGCTTATGTAGTAAATGAAGACCA
AAAAAGGTTTTTATTTAAATACGATTCAAGAGGCGAACTTGCCACACGTGATATTGTTTCACACGCCATTCATAAAGAAA
TGCATGAAACACAAAAAAAACATGTTTATCTCGATTGCAGGCATTTAGATAAAAAAGCTTTCTACAAAAAGTTTCCCGCT
ATAACAGCACATTGCAATGAACTGGGATTGTTTCCTGAAAAAGATTTGATTCCTATAGTTCCTGCAGCACATTATCAATG
CGGCGGCATAAAGGTAGACCGTAATGGAGCAACAATAATTCCTAATTTATATGCGGTTGGCGAATGCGCCAGAACGGGAC
TTCATGGAAAAAACCGTCTGGCTTCTAATTCCCTGCTCGAAGCTTTGGTATTTGCACATCAGGCATCAGAGAATATAGAC
AAAACAATAGATTCGTTTCAGTTTTCATCAAAACTGCTTATTCCAAAATTTCCCGATTCGGAAATCAAAACTGATTATGT
TGCCTTTTTAATTTTAAAAAAAGAAATACAGGCACTTATAACCAATTTTTATTCCAGCGAAGAACGCAATACCGATTATG
CAATTCAAAAAATAAAATTGATTAAAAATGCAGCCGAAACCCTTATACACGGCCAGGAAATTACAATTCCGTTTATAGAA
TTTTCTAATATGCTTACCGTGGCCTTAATTATTATTAAACACTGCAAAAAAAGAAAAGCCCCATATATTTTGTCTTTAAA
CTAA

Upstream 100 bases:

>100_bases
ACGAAAGCCCGGAAATCCTGGTAAACGAACCGTTAAGGATCGAAGCACTGAAACCTATACAAAGAATGCTTGAATTGTCT
TAAACTTAAAAACAAAGAAA

Downstream 100 bases:

>100_bases
AAACCAATATTATGAAAATAACCAGTAAAACTACAATTGGCGAGATCGCAGCAAATGATTTTAGAACAGCAGCAATTTTT
ACAAAATACAATATCGATTT

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 507; Mature: 507

Protein sequence:

>507_residues
MLKTDILIVGSGISGLFLAMKTAKKRPDLSIVIMTKKTAGNTNTQLAQGGIAVVTNHLQDSFEQHIKDTLKSGGGHCDEE
IVKMVVQQAPERLKELIEIGTSFDKNQNGQWDLGLEGGHSQHRILHHKDRSGLEIEKKLLHIITELPNIKLFENYQVVDL
NTETKENETHCTGAFFYDKNENKIKYIRARSIILSTGGCGTLFENTTNPEIATGDGIAIAARAGVEIEDMQYIQFHPTAL
YTGKKSQLFLISEAVRGFGAYVVNEDQKRFLFKYDSRGELATRDIVSHAIHKEMHETQKKHVYLDCRHLDKKAFYKKFPA
ITAHCNELGLFPEKDLIPIVPAAHYQCGGIKVDRNGATIIPNLYAVGECARTGLHGKNRLASNSLLEALVFAHQASENID
KTIDSFQFSSKLLIPKFPDSEIKTDYVAFLILKKEIQALITNFYSSEERNTDYAIQKIKLIKNAAETLIHGQEITIPFIE
FSNMLTVALIIIKHCKKRKAPYILSLN

Sequences:

>Translated_507_residues
MLKTDILIVGSGISGLFLAMKTAKKRPDLSIVIMTKKTAGNTNTQLAQGGIAVVTNHLQDSFEQHIKDTLKSGGGHCDEE
IVKMVVQQAPERLKELIEIGTSFDKNQNGQWDLGLEGGHSQHRILHHKDRSGLEIEKKLLHIITELPNIKLFENYQVVDL
NTETKENETHCTGAFFYDKNENKIKYIRARSIILSTGGCGTLFENTTNPEIATGDGIAIAARAGVEIEDMQYIQFHPTAL
YTGKKSQLFLISEAVRGFGAYVVNEDQKRFLFKYDSRGELATRDIVSHAIHKEMHETQKKHVYLDCRHLDKKAFYKKFPA
ITAHCNELGLFPEKDLIPIVPAAHYQCGGIKVDRNGATIIPNLYAVGECARTGLHGKNRLASNSLLEALVFAHQASENID
KTIDSFQFSSKLLIPKFPDSEIKTDYVAFLILKKEIQALITNFYSSEERNTDYAIQKIKLIKNAAETLIHGQEITIPFIE
FSNMLTVALIIIKHCKKRKAPYILSLN
>Mature_507_residues
MLKTDILIVGSGISGLFLAMKTAKKRPDLSIVIMTKKTAGNTNTQLAQGGIAVVTNHLQDSFEQHIKDTLKSGGGHCDEE
IVKMVVQQAPERLKELIEIGTSFDKNQNGQWDLGLEGGHSQHRILHHKDRSGLEIEKKLLHIITELPNIKLFENYQVVDL
NTETKENETHCTGAFFYDKNENKIKYIRARSIILSTGGCGTLFENTTNPEIATGDGIAIAARAGVEIEDMQYIQFHPTAL
YTGKKSQLFLISEAVRGFGAYVVNEDQKRFLFKYDSRGELATRDIVSHAIHKEMHETQKKHVYLDCRHLDKKAFYKKFPA
ITAHCNELGLFPEKDLIPIVPAAHYQCGGIKVDRNGATIIPNLYAVGECARTGLHGKNRLASNSLLEALVFAHQASENID
KTIDSFQFSSKLLIPKFPDSEIKTDYVAFLILKKEIQALITNFYSSEERNTDYAIQKIKLIKNAAETLIHGQEITIPFIE
FSNMLTVALIIIKHCKKRKAPYILSLN

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=402, Percent_Identity=31.0945273631841, Blast_Score=186, Evalue=4e-47,
Organism=Escherichia coli, GI1788928, Length=506, Percent_Identity=35.7707509881423, Blast_Score=317, Evalue=9e-88,
Organism=Escherichia coli, GI1790597, Length=389, Percent_Identity=33.1619537275064, Blast_Score=211, Evalue=1e-55,
Organism=Escherichia coli, GI1786942, Length=405, Percent_Identity=30.8641975308642, Blast_Score=181, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI17550100, Length=419, Percent_Identity=30.7875894988067, Blast_Score=192, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI17505833, Length=422, Percent_Identity=30.8056872037915, Blast_Score=189, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI71986328, Length=433, Percent_Identity=27.7136258660508, Blast_Score=100, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6322701, Length=414, Percent_Identity=30.6763285024155, Blast_Score=187, Evalue=4e-48,
Organism=Saccharomyces cerevisiae, GI6322416, Length=392, Percent_Identity=30.1020408163265, Blast_Score=178, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6320788, Length=491, Percent_Identity=26.2729124236253, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6322511, Length=322, Percent_Identity=27.639751552795, Blast_Score=88, Evalue=4e-18,
Organism=Drosophila melanogaster, GI17137288, Length=397, Percent_Identity=31.7380352644836, Blast_Score=192, Evalue=4e-49,
Organism=Drosophila melanogaster, GI24655642, Length=397, Percent_Identity=31.7380352644836, Blast_Score=192, Evalue=4e-49,
Organism=Drosophila melanogaster, GI24655647, Length=397, Percent_Identity=31.7380352644836, Blast_Score=192, Evalue=4e-49,
Organism=Drosophila melanogaster, GI24663005, Length=514, Percent_Identity=25.4863813229572, Blast_Score=163, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 56893; Mature: 56893

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKTDILIVGSGISGLFLAMKTAKKRPDLSIVIMTKKTAGNTNTQLAQGGIAVVTNHLQD
CCCEEEEEEECCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCHHHCCCHHHHHHHHHH
SFEQHIKDTLKSGGGHCDEEIVKMVVQQAPERLKELIEIGTSFDKNQNGQWDLGLEGGHS
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCC
QHRILHHKDRSGLEIEKKLLHIITELPNIKLFENYQVVDLNTETKENETHCTGAFFYDKN
CCCEEECCCCCCCHHHHHHHHHHHHCCCCEEECCEEEEECCCCCCCCCCEEEEEEEEECC
ENKIKYIRARSIILSTGGCGTLFENTTNPEIATGDGIAIAARAGVEIEDMQYIQFHPTAL
CCEEEEEEEEEEEEECCCCCHHCCCCCCCCEECCCCEEEEEECCCEECCCEEEEEECEEE
YTGKKSQLFLISEAVRGFGAYVVNEDQKRFLFKYDSRGELATRDIVSHAIHKEMHETQKK
ECCCCCEEEEEHHHHHHCCEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHC
HVYLDCRHLDKKAFYKKFPAITAHCNELGLFPEKDLIPIVPAAHYQCGGIKVDRNGATII
EEEEEHHHCCHHHHHHHCCCCEECHHHCCCCCCCCCEECCCCCCEECCCEEECCCCCEEC
PNLYAVGECARTGLHGKNRLASNSLLEALVFAHQASENIDKTIDSFQFSSKLLIPKFPDS
CCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCC
EIKTDYVAFLILKKEIQALITNFYSSEERNTDYAIQKIKLIKNAAETLIHGQEITIPFIE
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEHHH
FSNMLTVALIIIKHCKKRKAPYILSLN
HHHHHHHHHHHHHHHHHCCCCEEEEEC
>Mature Secondary Structure
MLKTDILIVGSGISGLFLAMKTAKKRPDLSIVIMTKKTAGNTNTQLAQGGIAVVTNHLQD
CCCEEEEEEECCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCHHHCCCHHHHHHHHHH
SFEQHIKDTLKSGGGHCDEEIVKMVVQQAPERLKELIEIGTSFDKNQNGQWDLGLEGGHS
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCC
QHRILHHKDRSGLEIEKKLLHIITELPNIKLFENYQVVDLNTETKENETHCTGAFFYDKN
CCCEEECCCCCCCHHHHHHHHHHHHCCCCEEECCEEEEECCCCCCCCCCEEEEEEEEECC
ENKIKYIRARSIILSTGGCGTLFENTTNPEIATGDGIAIAARAGVEIEDMQYIQFHPTAL
CCEEEEEEEEEEEEECCCCCHHCCCCCCCCEECCCCEEEEEECCCEECCCEEEEEECEEE
YTGKKSQLFLISEAVRGFGAYVVNEDQKRFLFKYDSRGELATRDIVSHAIHKEMHETQKK
ECCCCCEEEEEHHHHHHCCEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHC
HVYLDCRHLDKKAFYKKFPAITAHCNELGLFPEKDLIPIVPAAHYQCGGIKVDRNGATII
EEEEEHHHCCHHHHHHHCCCCEECHHHCCCCCCCCCEECCCCCCEECCCEEECCCCCEEC
PNLYAVGECARTGLHGKNRLASNSLLEALVFAHQASENIDKTIDSFQFSSKLLIPKFPDS
CCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCC
EIKTDYVAFLILKKEIQALITNFYSSEERNTDYAIQKIKLIKNAAETLIHGQEITIPFIE
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEHHH
FSNMLTVALIIIKHCKKRKAPYILSLN
HHHHHHHHHHHHHHHHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]