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The map label for this gene is icaA [H]

Identifier: 146301659

GI number: 146301659

Start: 4670139

End: 4671572

Strand: Reverse

Name: icaA [H]

Synonym: Fjoh_3921

Alternate gene names: 146301659

Gene position: 4671572-4670139 (Counterclockwise)

Preceding gene: 146301660

Following gene: 146301655

Centisome position: 76.62

GC content: 34.24

Gene sequence:

>1434_bases
ATGACTTTTTTTAATCACGAAATAACAATCTTTACCCACATATATTTAATTCTCATATTGGCTTATGCGATATTGATTAT
GTCGTCTTATCTCATATTGGCTTATCTTTCCGGGAAGGAATTAAGAGGCTATCTAAAAAAGAATAGTTTTATAGATTATG
ATGTACTTCTAACAAGTGAATTTGCTCCTAAACTTTCCTTAATTGCACCTGCTTATAACGAAGGTTTTACTATTGAAGAA
AATGTAAAATCGCTGCTTTCACTAAACTATAACCAATATGAAGTAATTGTTGTTAACGATGGAAGCAAGGACAATTCTAT
GGAGGTTTTAATTAATACCTATGATCTTGTCCTTACAGAACTTGCTTTTCATCAGCAGATTGAAACAAAAAAAATCAGAG
GACTTTATGTTTCCAGAAATGCCGCTTTTAAAAAATTAATTGTAGTTGATAAAGAAAATGGAGGTAAAGCCGATGCTTTA
AATGCCGGACTTAATATTGCACAAAATCCGTATGTTGTTTGTATTGATGTTGATTGTATTCTTGATAAAGATTCACTTCT
AAAACTTGCAAAACCATTTCTGGAATCCAGTGGACAACGTATTATTGCAACGGGCGGTGTGGTGAGAATTGCAAACCAAT
GTATTATTAAAAACGGACGTTTGATAGAAGTAAATGTACCGGATCTTTTACTGCCAAGAATTCAGGTTTTAGAATATCTA
AGAGCTTTTCTTTTGGGAAGAATGGCGTGGGGCAGACTCGATGGATTATTACTAATAAGCGGTGCGTTTGGTGCATTTGA
TAAAGAAATAGCATTGCTTGCAGGCGGTTACAGCACCAAAACAGTAGGAGAGGATATGGAACTTGTGGTAAGAATGCGCC
GTTATATGCTCGAAAATAATTTGCCTTATTCTGTGCGATACATTCCAGATCCGCTTTGCTGGACCGAAGCTCCTGAAGAT
TTTAAAATATTTAAAAAACAGCGCAGCCGATGGATGAGAGGAACAATTGAAACATTAGATTTTCATAAAAAAATGTTCCT
GAATCCAAAATATAAATTGCTGGGAATGCTGAGTATTCCGTATTGGACTTTATTTGAATTTCTGGCTCCTGGTATTGAGT
TTATCGGACTTGTAATTACTATTGTATTTTTCATATTTGGACTGCTCAATTGGCATTTTTTCCTTCTGTTATTATTGTTT
GTATACACTTTTGCTGTTTTCTTTTCGGTTATTGCTTTGTACAGCGAAGAACAAACGTATCATAAATACCCAAAACAAAG
AGATTTTTTCAAGCTTTTACTAGCCGCTTTTATCGAGCCTTTTTATTTTCACCCTTTAACGGTTTATGCCGCTTTAATTG
GTTATAAAGAAAAAGTAATGGGAACCAAAGGCTGGGGCGAAATGACTAGAAAAGGTTTTACAAAAAAAGAATAA

Upstream 100 bases:

>100_bases
TTCTTATGTTGAGCAATTAACAGAAAGGACTTCAAATACATTAATAAAGTATGAAGCTCAAAATGTTCTGCATACACTAA
CAGTTTAACAAGCATTGCTG

Downstream 100 bases:

>100_bases
TTTTTACGGTTTTAAATTTTCCTTTAAATAAAAATCCCCTAGTTTCCAGGCATAGAAACTAGGGGAAATTAAAAAAAATA
AAACACAATCTTTAGAACGA

Product: glycosyl transferase family protein

Products: NA

Alternate protein names: PNAG synthase; Poly-beta-1,6-GlcNAc synthase; Biofilm polysaccharide intercellular adhesin synthesis protein IcaA; Biofilm PIA synthesis protein IcaA; Intercellular adhesion protein A; N-acetylglucosaminyltransferase IcaA [H]

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MTFFNHEITIFTHIYLILILAYAILIMSSYLILAYLSGKELRGYLKKNSFIDYDVLLTSEFAPKLSLIAPAYNEGFTIEE
NVKSLLSLNYNQYEVIVVNDGSKDNSMEVLINTYDLVLTELAFHQQIETKKIRGLYVSRNAAFKKLIVVDKENGGKADAL
NAGLNIAQNPYVVCIDVDCILDKDSLLKLAKPFLESSGQRIIATGGVVRIANQCIIKNGRLIEVNVPDLLLPRIQVLEYL
RAFLLGRMAWGRLDGLLLISGAFGAFDKEIALLAGGYSTKTVGEDMELVVRMRRYMLENNLPYSVRYIPDPLCWTEAPED
FKIFKKQRSRWMRGTIETLDFHKKMFLNPKYKLLGMLSIPYWTLFEFLAPGIEFIGLVITIVFFIFGLLNWHFFLLLLLF
VYTFAVFFSVIALYSEEQTYHKYPKQRDFFKLLLAAFIEPFYFHPLTVYAALIGYKEKVMGTKGWGEMTRKGFTKKE

Sequences:

>Translated_477_residues
MTFFNHEITIFTHIYLILILAYAILIMSSYLILAYLSGKELRGYLKKNSFIDYDVLLTSEFAPKLSLIAPAYNEGFTIEE
NVKSLLSLNYNQYEVIVVNDGSKDNSMEVLINTYDLVLTELAFHQQIETKKIRGLYVSRNAAFKKLIVVDKENGGKADAL
NAGLNIAQNPYVVCIDVDCILDKDSLLKLAKPFLESSGQRIIATGGVVRIANQCIIKNGRLIEVNVPDLLLPRIQVLEYL
RAFLLGRMAWGRLDGLLLISGAFGAFDKEIALLAGGYSTKTVGEDMELVVRMRRYMLENNLPYSVRYIPDPLCWTEAPED
FKIFKKQRSRWMRGTIETLDFHKKMFLNPKYKLLGMLSIPYWTLFEFLAPGIEFIGLVITIVFFIFGLLNWHFFLLLLLF
VYTFAVFFSVIALYSEEQTYHKYPKQRDFFKLLLAAFIEPFYFHPLTVYAALIGYKEKVMGTKGWGEMTRKGFTKKE
>Mature_476_residues
TFFNHEITIFTHIYLILILAYAILIMSSYLILAYLSGKELRGYLKKNSFIDYDVLLTSEFAPKLSLIAPAYNEGFTIEEN
VKSLLSLNYNQYEVIVVNDGSKDNSMEVLINTYDLVLTELAFHQQIETKKIRGLYVSRNAAFKKLIVVDKENGGKADALN
AGLNIAQNPYVVCIDVDCILDKDSLLKLAKPFLESSGQRIIATGGVVRIANQCIIKNGRLIEVNVPDLLLPRIQVLEYLR
AFLLGRMAWGRLDGLLLISGAFGAFDKEIALLAGGYSTKTVGEDMELVVRMRRYMLENNLPYSVRYIPDPLCWTEAPEDF
KIFKKQRSRWMRGTIETLDFHKKMFLNPKYKLLGMLSIPYWTLFEFLAPGIEFIGLVITIVFFIFGLLNWHFFLLLLLFV
YTFAVFFSVIALYSEEQTYHKYPKQRDFFKLLLAAFIEPFYFHPLTVYAALIGYKEKVMGTKGWGEMTRKGFTKKE

Specific function: N-acetylglucosaminyltransferase that catalyzes the polymerization of single monomer units of UDP-N-acetylglucosamine to produce the linear homopolymer poly-beta-1,6-N-acetyl-D- glucosamine (PNAG, also referred to as PIA), a biofilm adhesin polysaccharide.

COG id: COG1215

COG function: function code M; Glycosyltransferases, probably involved in cell wall biogenesis

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787259, Length=283, Percent_Identity=28.6219081272085, Blast_Score=104, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: NA

Molecular weight: Translated: 54810; Mature: 54679

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFFNHEITIFTHIYLILILAYAILIMSSYLILAYLSGKELRGYLKKNSFIDYDVLLTSE
CCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEEEEECC
FAPKLSLIAPAYNEGFTIEENVKSLLSLNYNQYEVIVVNDGSKDNSMEVLINTYDLVLTE
CCCCEEEECCCCCCCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEEEEHHHHHHHH
LAFHQQIETKKIRGLYVSRNAAFKKLIVVDKENGGKADALNAGLNIAQNPYVVCIDVDCI
HHHHHHHHHHHHCEEEEECCCCEEEEEEEECCCCCCCHHHHCCCCCCCCCEEEEEEEEEE
LDKDSLLKLAKPFLESSGQRIIATGGVVRIANQCIIKNGRLIEVNVPDLLLPRIQVLEYL
ECHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHH
RAFLLGRMAWGRLDGLLLISGAFGAFDKEIALLAGGYSTKTVGEDMELVVRMRRYMLENN
HHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCC
LPYSVRYIPDPLCWTEAPEDFKIFKKQRSRWMRGTIETLDFHKKMFLNPKYKLLGMLSIP
CCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEECCC
YWTLFEFLAPGIEFIGLVITIVFFIFGLLNWHFFLLLLLFVYTFAVFFSVIALYSEEQTY
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
HKYPKQRDFFKLLLAAFIEPFYFHPLTVYAALIGYKEKVMGTKGWGEMTRKGFTKKE
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCC
>Mature Secondary Structure 
TFFNHEITIFTHIYLILILAYAILIMSSYLILAYLSGKELRGYLKKNSFIDYDVLLTSE
CEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEEEEECC
FAPKLSLIAPAYNEGFTIEENVKSLLSLNYNQYEVIVVNDGSKDNSMEVLINTYDLVLTE
CCCCEEEECCCCCCCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEEEEHHHHHHHH
LAFHQQIETKKIRGLYVSRNAAFKKLIVVDKENGGKADALNAGLNIAQNPYVVCIDVDCI
HHHHHHHHHHHHCEEEEECCCCEEEEEEEECCCCCCCHHHHCCCCCCCCCEEEEEEEEEE
LDKDSLLKLAKPFLESSGQRIIATGGVVRIANQCIIKNGRLIEVNVPDLLLPRIQVLEYL
ECHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHH
RAFLLGRMAWGRLDGLLLISGAFGAFDKEIALLAGGYSTKTVGEDMELVVRMRRYMLENN
HHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCC
LPYSVRYIPDPLCWTEAPEDFKIFKKQRSRWMRGTIETLDFHKKMFLNPKYKLLGMLSIP
CCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEECCC
YWTLFEFLAPGIEFIGLVITIVFFIFGLLNWHFFLLLLLFVYTFAVFFSVIALYSEEQTY
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
HKYPKQRDFFKLLLAAFIEPFYFHPLTVYAALIGYKEKVMGTKGWGEMTRKGFTKKE
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10496925 [H]