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The map label for this gene is insB1 [H]

Identifier: 146301353

GI number: 146301353

Start: 4309411

End: 4310070

Strand: Reverse

Name: insB1 [H]

Synonym: Fjoh_3611

Alternate gene names: 146301353

Gene position: 4310070-4309411 (Counterclockwise)

Preceding gene: 146301354

Following gene: 146301352

Centisome position: 70.69

GC content: 32.27

Gene sequence:

>660_bases
ATGAAAATAAAATGTAATTTTTGCAATGGTAAATGCATAAAAAATGGATTACAATCTAACGGAAATCAGCGTTATAAATG
TTGTGCTTGCAAAAAAAGACAGCAGATTGAATATAGCTACAACGCTTACAAAAAGGATATCAATCAGGAAATAGTGCTGT
TCACCAGAGAAGGATTAGGAATAAGAAGCACGGCAAGAATATTGAAAATATCAGCTACAACATTACTAAAAAGAATTGTT
TCAATTGCTAGAAACATTACCAAGCCAATTATCAGCAAAGGTAAAACTTATGAAGTCGATGAACTTTGCACTTATATCAG
ACACAAGAAAAATTATATCTGGCTGGTTTATGCATTGGAAAAGAATTCTAGAACTGTTGTAAGTTTTAGCGTTGGAAAAC
GAACCAATAAAACGCTGAATCGTGTTTTGGAAACTTTAAAATTATCAGAAGCTAAAAAGATATTTACAGACAGATTGAAA
AATTATCGATATCTGCTTGATCAGGAAATGCATTCTGTCAAGCGATTTGGGACAAATCATATTGAAAGAAAGAATCTGAC
GCTGAGAACTCATCTGAAAAGACTCAACCGCAGGACAATCTGCTTCAGTAAAAGTTTGGTTATTTTTACTGCTGTTATGA
AGATTTATTTTTGGTTTTGA

Upstream 100 bases:

>100_bases
GAATAGAAAAAGAGACGACCATCAGGTCGTCTCTTTCATTTTTTGTCTTTTAATAAACCTTAAAATGCTTTTACCTTACC
TTAGTGTTTCAAATTAGGTC

Downstream 100 bases:

>100_bases
GCAATAAAACAGTAAGTTTATCCTTTAAAATCATGTTCTTAAAAGGTTTGCATTTAACAGGTAAAAACTTGTAAAAATAG
TAATAAACCCTTATAAGTAT

Product: IS1 transposase

Products: NA

Alternate protein names: IS1a [H]

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MKIKCNFCNGKCIKNGLQSNGNQRYKCCACKKRQQIEYSYNAYKKDINQEIVLFTREGLGIRSTARILKISATTLLKRIV
SIARNITKPIISKGKTYEVDELCTYIRHKKNYIWLVYALEKNSRTVVSFSVGKRTNKTLNRVLETLKLSEAKKIFTDRLK
NYRYLLDQEMHSVKRFGTNHIERKNLTLRTHLKRLNRRTICFSKSLVIFTAVMKIYFWF

Sequences:

>Translated_219_residues
MKIKCNFCNGKCIKNGLQSNGNQRYKCCACKKRQQIEYSYNAYKKDINQEIVLFTREGLGIRSTARILKISATTLLKRIV
SIARNITKPIISKGKTYEVDELCTYIRHKKNYIWLVYALEKNSRTVVSFSVGKRTNKTLNRVLETLKLSEAKKIFTDRLK
NYRYLLDQEMHSVKRFGTNHIERKNLTLRTHLKRLNRRTICFSKSLVIFTAVMKIYFWF
>Mature_219_residues
MKIKCNFCNGKCIKNGLQSNGNQRYKCCACKKRQQIEYSYNAYKKDINQEIVLFTREGLGIRSTARILKISATTLLKRIV
SIARNITKPIISKGKTYEVDELCTYIRHKKNYIWLVYALEKNSRTVVSFSVGKRTNKTLNRVLETLKLSEAKKIFTDRLK
NYRYLLDQEMHSVKRFGTNHIERKNLTLRTHLKRLNRRTICFSKSLVIFTAVMKIYFWF

Specific function: Absolutely required for transposition of IS1 [H]

COG id: COG1662

COG function: function code L; Transposase and inactivated derivatives, IS1 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 27 family [H]

Homologues:

Organism=Escherichia coli, GI1787223, Length=120, Percent_Identity=35.8333333333333, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1789853, Length=120, Percent_Identity=35.8333333333333, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1788203, Length=120, Percent_Identity=35.8333333333333, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1786203, Length=120, Percent_Identity=35.8333333333333, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1786470, Length=120, Percent_Identity=35.8333333333333, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1786459, Length=120, Percent_Identity=35.8333333333333, Blast_Score=74, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005063 [H]

Pfam domain/function: PF03400 Transposase_27 [H]

EC number: NA

Molecular weight: Translated: 25883; Mature: 25883

Theoretical pI: Translated: 10.82; Mature: 10.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKCNFCNGKCIKNGLQSNGNQRYKCCACKKRQQIEYSYNAYKKDINQEIVLFTREGLG
CEEEEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
IRSTARILKISATTLLKRIVSIARNITKPIISKGKTYEVDELCTYIRHKKNYIWLVYALE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCEEEEEEEEE
KNSRTVVSFSVGKRTNKTLNRVLETLKLSEAKKIFTDRLKNYRYLLDQEMHSVKRFGTNH
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
IERKNLTLRTHLKRLNRRTICFSKSLVIFTAVMKIYFWF
HHHCCCHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKIKCNFCNGKCIKNGLQSNGNQRYKCCACKKRQQIEYSYNAYKKDINQEIVLFTREGLG
CEEEEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
IRSTARILKISATTLLKRIVSIARNITKPIISKGKTYEVDELCTYIRHKKNYIWLVYALE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCEEEEEEEEE
KNSRTVVSFSVGKRTNKTLNRVLETLKLSEAKKIFTDRLKNYRYLLDQEMHSVKRFGTNH
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
IERKNLTLRTHLKRLNRRTICFSKSLVIFTAVMKIYFWF
HHHCCCHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 375010; 1849492; 1630901; 9278503 [H]