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The map label for this gene is rlmH

Identifier: 146301237

GI number: 146301237

Start: 4171624

End: 4172097

Strand: Direct

Name: rlmH

Synonym: Fjoh_3495

Alternate gene names: 146301237

Gene position: 4171624-4172097 (Clockwise)

Preceding gene: 146301236

Following gene: 146301240

Centisome position: 68.42

GC content: 33.76

Gene sequence:

>474_bases
ATGAACATCAAACTCATCGCTATAGGCAAAACAGATAATAAATCGCTTCAAACTTTGATTGACGATTATACCAAACGTTT
ATCCTTTTATATCAAATTTGATCTGGAAATTATTCCTGACATTAAAAATGTAAAAAACTTATCTGAAAGCCAGCAAAAGG
AAAAAGAAGGCGAACTGATTCTGTCTAAACTTTCGGCAACTGATCAGCTTATTTTATTGGATGAAAACGGAAAAACCTTC
TCTAGTGTTGGTTTCTCAGAAGAACTCCAAAAGAAAATGAATTCTGGTGTAAAAACACTGGTTTTTGTAATTGGCGGACC
TTACGGATTCTCTGAAACGATTTATAAAAAAGCGCAGGGAAAAGTTTCGCTTTCGCTAATGACATTTTCGCATCAAATGG
TTCGCTTATTTTTTATTGAACAATTGTACCGCGGATTTACAATTTTACGCAACGAACCTTATCATCATCAATAA

Upstream 100 bases:

>100_bases
GATTTTATTTGCCACAGATTTCACAGATTTACACAGATTAAATTATCCTCAATTTAAAAATCCTTCTAATCTGTGAAATC
TGTGGCTAAAAGAAAAAACA

Downstream 100 bases:

>100_bases
TTTCTTGTTTCAGGTTTTCTTTGTTTCAGGTTTAAAAGATGAATTCTACCGGAAGTTTATCAAGATTATAAGTTACATCT
TTTTGCAGTAGAATTTTATA

Product: rRNA large subunit methyltransferase

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MNIKLIAIGKTDNKSLQTLIDDYTKRLSFYIKFDLEIIPDIKNVKNLSESQQKEKEGELILSKLSATDQLILLDENGKTF
SSVGFSEELQKKMNSGVKTLVFVIGGPYGFSETIYKKAQGKVSLSLMTFSHQMVRLFFIEQLYRGFTILRNEPYHHQ

Sequences:

>Translated_157_residues
MNIKLIAIGKTDNKSLQTLIDDYTKRLSFYIKFDLEIIPDIKNVKNLSESQQKEKEGELILSKLSATDQLILLDENGKTF
SSVGFSEELQKKMNSGVKTLVFVIGGPYGFSETIYKKAQGKVSLSLMTFSHQMVRLFFIEQLYRGFTILRNEPYHHQ
>Mature_157_residues
MNIKLIAIGKTDNKSLQTLIDDYTKRLSFYIKFDLEIIPDIKNVKNLSESQQKEKEGELILSKLSATDQLILLDENGKTF
SSVGFSEELQKKMNSGVKTLVFVIGGPYGFSETIYKKAQGKVSLSLMTFSHQMVRLFFIEQLYRGFTILRNEPYHHQ

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family

Homologues:

Organism=Escherichia coli, GI1786855, Length=157, Percent_Identity=31.8471337579618, Blast_Score=91, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMH_FLAJ1 (A5FE53)

Other databases:

- EMBL:   CP000685
- RefSeq:   YP_001195828.1
- ProteinModelPortal:   A5FE53
- SMR:   A5FE53
- STRING:   A5FE53
- GeneID:   5091173
- GenomeReviews:   CP000685_GR
- KEGG:   fjo:Fjoh_3495
- eggNOG:   COG1576
- HOGENOM:   HBG622807
- OMA:   TANHPYH
- ProtClustDB:   PRK00103
- BioCyc:   FJOH376686:FJOH_3495-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00658
- InterPro:   IPR003742
- PIRSF:   PIRSF004505

Pfam domain/function: PF02590 SPOUT_MTase

EC number: NA

Molecular weight: Translated: 18019; Mature: 18019

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIKLIAIGKTDNKSLQTLIDDYTKRLSFYIKFDLEIIPDIKNVKNLSESQQKEKEGELI
CCEEEEEEECCCCHHHHHHHHHHHHHEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHH
LSKLSATDQLILLDENGKTFSSVGFSEELQKKMNSGVKTLVFVIGGPYGFSETIYKKAQG
HHHHCCCCEEEEEECCCCEEHHCCCHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHCCC
KVSLSLMTFSHQMVRLFFIEQLYRGFTILRNEPYHHQ
CEEEEEEHHHHHHHHHHHHHHHHCCEEEEECCCCCCC
>Mature Secondary Structure
MNIKLIAIGKTDNKSLQTLIDDYTKRLSFYIKFDLEIIPDIKNVKNLSESQQKEKEGELI
CCEEEEEEECCCCHHHHHHHHHHHHHEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHH
LSKLSATDQLILLDENGKTFSSVGFSEELQKKMNSGVKTLVFVIGGPYGFSETIYKKAQG
HHHHCCCCEEEEEECCCCEEHHCCCHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHCCC
KVSLSLMTFSHQMVRLFFIEQLYRGFTILRNEPYHHQ
CEEEEEEHHHHHHHHHHHHHHHHCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA