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The map label for this gene is blaB1 [H]

Identifier: 146301227

GI number: 146301227

Start: 4163212

End: 4163958

Strand: Reverse

Name: blaB1 [H]

Synonym: Fjoh_3485

Alternate gene names: 146301227

Gene position: 4163958-4163212 (Counterclockwise)

Preceding gene: 146301228

Following gene: 146301226

Centisome position: 68.3

GC content: 33.2

Gene sequence:

>747_bases
ATGCGAAAATTAGCTTCGATAATTTTATTCTTAGCCGCGGTTTCAAATAGTTTGGGACAATCTAAGAATTCGCCATTACA
AATAAGTCATCTTACAGGTGACTTTTATGTTTATAGAACTTTTAATGATTACAAAGGAACTAAGATTTCTGCCAATGCTA
TGTATGTTGTTACAGATAAAGGCGTTGTGCTTTTTGATGCGCCTTGGGATAAAACACAGTTTCAGCCGTTATTAGACAGC
ATAAAAGCAAAACACAATAAAGAGGTTGTGATGCTTTTTGGCACGCATTCTCATGAAGATCGTGCAGGAGGATTTGATTT
TTACAAGAAAAAAGGAATCAAAACGTACTCAATTAAACTGACTGATGATATTCTTAAAAAGAATAAGGAACCAAGAGCAG
AATTTATAATTTCAAATGATACAACATTTACTGTTGGAAATCATACTTTTGAAGTTTATTACCCAGGAAAAGGACATGCT
CCTGATAATATTGTAGCATGGTTTAAAAAAGAGAAAATTCTTTACGGAGGCTGTTTTGTAAAAAGTGCAGAAGCATTAGA
TTTAGGTTATCTGGGTGATGCTGATGTTAAAGAATGGCAGAAATCTATAAAAAAAGTGCAGGCAAAATTCAAAAAACCGG
ATTATATAATTTCGGGACATGATGACTGGACTAGTAAAGAATCTTTAAATCATACTTTGAAATTGGTTGACGAGTATTTG
GCTCAAAAATCTGCCGGAAAAAAGTAA

Upstream 100 bases:

>100_bases
GAATCTGCAGATAAATTAAAACTTCGCGGTTATGTTGGAATTTCTTTAATGGGAAGAACACAATATTGGACACGAGTGAA
AAATTAATTCAAAATAATAC

Downstream 100 bases:

>100_bases
CTTTGCAATCCTATATAATAGTACATGTTTTTTATCGAAGTTATTTTACCGCTTTCCTTAGCAAAAACCTTTACATATCG
TGTTTCTGAGGCTGAATTCC

Product: beta-lactamase

Products: NA

Alternate protein names: CHbetaL-1; Class B carbapenemase BlaB-1 [H]

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MRKLASIILFLAAVSNSLGQSKNSPLQISHLTGDFYVYRTFNDYKGTKISANAMYVVTDKGVVLFDAPWDKTQFQPLLDS
IKAKHNKEVVMLFGTHSHEDRAGGFDFYKKKGIKTYSIKLTDDILKKNKEPRAEFIISNDTTFTVGNHTFEVYYPGKGHA
PDNIVAWFKKEKILYGGCFVKSAEALDLGYLGDADVKEWQKSIKKVQAKFKKPDYIISGHDDWTSKESLNHTLKLVDEYL
AQKSAGKK

Sequences:

>Translated_248_residues
MRKLASIILFLAAVSNSLGQSKNSPLQISHLTGDFYVYRTFNDYKGTKISANAMYVVTDKGVVLFDAPWDKTQFQPLLDS
IKAKHNKEVVMLFGTHSHEDRAGGFDFYKKKGIKTYSIKLTDDILKKNKEPRAEFIISNDTTFTVGNHTFEVYYPGKGHA
PDNIVAWFKKEKILYGGCFVKSAEALDLGYLGDADVKEWQKSIKKVQAKFKKPDYIISGHDDWTSKESLNHTLKLVDEYL
AQKSAGKK
>Mature_248_residues
MRKLASIILFLAAVSNSLGQSKNSPLQISHLTGDFYVYRTFNDYKGTKISANAMYVVTDKGVVLFDAPWDKTQFQPLLDS
IKAKHNKEVVMLFGTHSHEDRAGGFDFYKKKGIKTYSIKLTDDILKKNKEPRAEFIISNDTTFTVGNHTFEVYYPGKGHA
PDNIVAWFKKEKILYGGCFVKSAEALDLGYLGDADVKEWQKSIKKVQAKFKKPDYIISGHDDWTSKESLNHTLKLVDEYL
AQKSAGKK

Specific function: Hydrolyzes penicillins, cephalosporins (including cefoxitin), carbapenems and 6-beta-iodopenicillanate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-B beta-lactamase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001018
- InterPro:   IPR001279 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.5.2.6 [H]

Molecular weight: Translated: 28075; Mature: 28075

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLASIILFLAAVSNSLGQSKNSPLQISHLTGDFYVYRTFNDYKGTKISANAMYVVTDK
CHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEEECCCCCCEEECCEEEEEECC
GVVLFDAPWDKTQFQPLLDSIKAKHNKEVVMLFGTHSHEDRAGGFDFYKKKGIKTYSIKL
CEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHCCCEEEEEEE
TDDILKKNKEPRAEFIISNDTTFTVGNHTFEVYYPGKGHAPDNIVAWFKKEKILYGGCFV
EHHHHHCCCCCCCEEEEECCCEEEECCEEEEEEECCCCCCCCHHHEEEECCEEEEEEEEE
KSAEALDLGYLGDADVKEWQKSIKKVQAKFKKPDYIISGHDDWTSKESLNHTLKLVDEYL
ECCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHH
AQKSAGKK
HHHCCCCC
>Mature Secondary Structure
MRKLASIILFLAAVSNSLGQSKNSPLQISHLTGDFYVYRTFNDYKGTKISANAMYVVTDK
CHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEEECCCCCCEEECCEEEEEECC
GVVLFDAPWDKTQFQPLLDSIKAKHNKEVVMLFGTHSHEDRAGGFDFYKKKGIKTYSIKL
CEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHCCCEEEEEEE
TDDILKKNKEPRAEFIISNDTTFTVGNHTFEVYYPGKGHAPDNIVAWFKKEKILYGGCFV
EHHHHHCCCCCCCEEEEECCCEEEECCEEEEEEECCCCCCCCHHHEEEECCEEEEEEEEE
KSAEALDLGYLGDADVKEWQKSIKKVQAKFKKPDYIISGHDDWTSKESLNHTLKLVDEYL
ECCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHH
AQKSAGKK
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9576862; 10858348 [H]