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The map label for this gene is priA [H]

Identifier: 146301226

GI number: 146301226

Start: 4160740

End: 4163187

Strand: Reverse

Name: priA [H]

Synonym: Fjoh_3484

Alternate gene names: 146301226

Gene position: 4163187-4160740 (Counterclockwise)

Preceding gene: 146301227

Following gene: 146301224

Centisome position: 68.28

GC content: 34.93

Gene sequence:

>2448_bases
ATGTTTTTTATCGAAGTTATTTTACCGCTTTCCTTAGCAAAAACCTTTACATATCGTGTTTCTGAGGCTGAATTCCATTT
TATTAAAAAAGGAATGCGGGTAGCGGTGCCATTTGGTAAAAGCAAAATTTATACAGCACTTGTATTAGATATACATGAAA
ATGCGCCAACCTTATATGAAGCCAAAGAAATTCATCAAATTTTAGATGAAAGGCCAATTGCAACCGAAATCCAGATTAAA
CACTGGCTTTGGGTAGCTAATTATTACATGTGCGGTATTGGCGATGTATATCGCGGCGCTTTTCCAACGGGATTACTGTT
GGAAAGTGAAACTATTGTTTCGCACAAACCAGATGTTGTTGTTAATGATTCTGAACTTTCTGATGATGAATTTTTAATTT
ATGAAGCGTTACAGCATCAGAGTTCGCTTAAAGTTCAGGAAATAGCTTCTATTTTAAATAGAAAAAATATCCTCCCAATT
CTTCAAAAATTAATAGCAAGAGATATAATTTTTCTGGAAGAAGAAATAAAAGAAAGTTATAAGCCTAAACTGGTTCGTTA
TGTTAAGCTGCACTCAAAATACGAAACAGATCAAGGTTTGGGAAGCCTTCTTGATATTCTGAAAAATGACAAGCAAAAAG
AAATTGTTTTAGCTTATTTTCAAATTAGTGCTTCAGAAAAAAAGCCTGTTACTGTTAAAAAATTAGTTGAGGTTTCTGGT
TCTAGTTCGTCTGTTGTAAAAACTTTAGTTAAGAATGAAATTCTTGAAGAATATCTTTTGCAGCAAGACCGTGTTTTGTT
TACCGGAGAAAAATCAGAAAAAGAATTATTGTTAAGTGAAGCGCAGCAAAATGCTTTTACAGCAATAAAACAAAGTCTTA
CTGAAAAAGAAGTCTGTTTGCTTCATGGAGTTACTTCGAGCGGGAAAACAGAAATTTACATAAAACTAATCGAAGAATAT
CTGCATACAGGAAGACAGGTTTTGTATCTGCTTCCTGAAATAGCCCTTACCACTCAGTTAGTTTCTCGTCTGCGTCTTCA
TTTTGGAGACAAAGTTGCTGTTTTTCATTCAAAATACAGTAATAATGAAAGAGTTGAGGTTTGGAAACAGACACTTGAAA
ATTCTGAAAAAGCTCAAATAGTCATAGGGGCAAGATCGGCTTTGTTTTTGCCTTTTAATGATCTTGGTCTGCTCATTGTT
GATGAAGAGCATGAGCAGACTTTTAAACAAACAGATCCGGCACCAAGATATCACGCCAGAGATGCTGCAATTGTATTAGC
AAATTTTCATAATGCTAAAGTTTTGCTTGGATCTGCAACACCAAGTATCGAGACTTATTTTAATACCCAGAATGATAAAT
ATGGTTTAGTAACACTTACTGAACGCTATAAAAATGTTCGACTGCCCGAAGTTGTTTTAGTCGATTTAAAAGACAAACAT
TTTAGGAAGAGAATGACAGGGCATTTTAGTGATCTTTTAATAGAAGAAATTACTGAGGCTTTGTCTTTGGGTGAACAAGT
GATTTTGTTTCAAAACAGAAGAGGATATTCACCTATAATAGAGTGTTTGACCTGCGGGCACGTACCTCATTGCCAGCAAT
GCGATGTGAGTTTAACGTATCATAAGTTTAAAAATCAGCTGCGTTGTCATTATTGTGGATACGCTATTGCAAAACCAACG
AATTGTCATAGCTGTTCAAGTATAGATTTAACCACAAAAGGGTTTGGAACAGAGCAGATAGAGCAGGAGCTGGCATCACT
TTTTCCGAAAGCAAAAACGGCACGTATGGATCAGGATACAACACGCGGTAAATTTGGTTTTGAAAAAATTATTGACACCT
TTAAAAATCGAGAAATTGATATTCTTGTCGGGACGCAAATGCTGGCAAAAGGGCTTGACTTTGATAATGTAAGTTTAGTG
GGAATCATGAATGCTGATAATATGCTGCATCATCCGGATTTTAGGGCTTTTGAACGCAGTTTTCAAATGATGACTCAGGT
TGCAGGAAGAGCAGGGAGATCTGAAAAACAGGGAAAAGTTGTTATTCAGACCTATAATCCAAATCATAATACAATTCAGC
AGGTTACTAACCATAATTACAATGGTATGTATAAGGAGCAGCTGTATGACAGGCAGATCTATAAATATCCTCCTTACTTC
AGAATTATAAAACTTACTATAAAACATAAAGATTTTGATAAGCTGAAAGAAGGCTCAATGTGGCTGTATCAGGTTTTAAG
CCAAAATCTAAATATACCTGTTTTGGGACCTGAAGAACCGGCAATCAGCAGAATAAGAAATGAATATATCCGAACAATTT
TAATTAAGATTCCGCAGAATATGCATTTGGGTAATACAAAAAAAACTATTCAGAAAATGTTGAATAGTTTTGAAGCTGTT
GCTCAGTACAGAGCTATAAAAGTGGTTGTAAATGTCGATTTTTATTAA

Upstream 100 bases:

>100_bases
TAGTAAAGAATCTTTAAATCATACTTTGAAATTGGTTGACGAGTATTTGGCTCAAAAATCTGCCGGAAAAAAGTAACTTT
GCAATCCTATATAATAGTAC

Downstream 100 bases:

>100_bases
AATTACGACGTTGACAAAGCTTTTACTAAATCTTCTTTTTTATTTCGGCTAAGCGGAATTTTAGTGATGCCGATTTCGGC
AAATTTACTATTGAAACGTT

Product: primosomal protein N'

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 815; Mature: 815

Protein sequence:

>815_residues
MFFIEVILPLSLAKTFTYRVSEAEFHFIKKGMRVAVPFGKSKIYTALVLDIHENAPTLYEAKEIHQILDERPIATEIQIK
HWLWVANYYMCGIGDVYRGAFPTGLLLESETIVSHKPDVVVNDSELSDDEFLIYEALQHQSSLKVQEIASILNRKNILPI
LQKLIARDIIFLEEEIKESYKPKLVRYVKLHSKYETDQGLGSLLDILKNDKQKEIVLAYFQISASEKKPVTVKKLVEVSG
SSSSVVKTLVKNEILEEYLLQQDRVLFTGEKSEKELLLSEAQQNAFTAIKQSLTEKEVCLLHGVTSSGKTEIYIKLIEEY
LHTGRQVLYLLPEIALTTQLVSRLRLHFGDKVAVFHSKYSNNERVEVWKQTLENSEKAQIVIGARSALFLPFNDLGLLIV
DEEHEQTFKQTDPAPRYHARDAAIVLANFHNAKVLLGSATPSIETYFNTQNDKYGLVTLTERYKNVRLPEVVLVDLKDKH
FRKRMTGHFSDLLIEEITEALSLGEQVILFQNRRGYSPIIECLTCGHVPHCQQCDVSLTYHKFKNQLRCHYCGYAIAKPT
NCHSCSSIDLTTKGFGTEQIEQELASLFPKAKTARMDQDTTRGKFGFEKIIDTFKNREIDILVGTQMLAKGLDFDNVSLV
GIMNADNMLHHPDFRAFERSFQMMTQVAGRAGRSEKQGKVVIQTYNPNHNTIQQVTNHNYNGMYKEQLYDRQIYKYPPYF
RIIKLTIKHKDFDKLKEGSMWLYQVLSQNLNIPVLGPEEPAISRIRNEYIRTILIKIPQNMHLGNTKKTIQKMLNSFEAV
AQYRAIKVVVNVDFY

Sequences:

>Translated_815_residues
MFFIEVILPLSLAKTFTYRVSEAEFHFIKKGMRVAVPFGKSKIYTALVLDIHENAPTLYEAKEIHQILDERPIATEIQIK
HWLWVANYYMCGIGDVYRGAFPTGLLLESETIVSHKPDVVVNDSELSDDEFLIYEALQHQSSLKVQEIASILNRKNILPI
LQKLIARDIIFLEEEIKESYKPKLVRYVKLHSKYETDQGLGSLLDILKNDKQKEIVLAYFQISASEKKPVTVKKLVEVSG
SSSSVVKTLVKNEILEEYLLQQDRVLFTGEKSEKELLLSEAQQNAFTAIKQSLTEKEVCLLHGVTSSGKTEIYIKLIEEY
LHTGRQVLYLLPEIALTTQLVSRLRLHFGDKVAVFHSKYSNNERVEVWKQTLENSEKAQIVIGARSALFLPFNDLGLLIV
DEEHEQTFKQTDPAPRYHARDAAIVLANFHNAKVLLGSATPSIETYFNTQNDKYGLVTLTERYKNVRLPEVVLVDLKDKH
FRKRMTGHFSDLLIEEITEALSLGEQVILFQNRRGYSPIIECLTCGHVPHCQQCDVSLTYHKFKNQLRCHYCGYAIAKPT
NCHSCSSIDLTTKGFGTEQIEQELASLFPKAKTARMDQDTTRGKFGFEKIIDTFKNREIDILVGTQMLAKGLDFDNVSLV
GIMNADNMLHHPDFRAFERSFQMMTQVAGRAGRSEKQGKVVIQTYNPNHNTIQQVTNHNYNGMYKEQLYDRQIYKYPPYF
RIIKLTIKHKDFDKLKEGSMWLYQVLSQNLNIPVLGPEEPAISRIRNEYIRTILIKIPQNMHLGNTKKTIQKMLNSFEAV
AQYRAIKVVVNVDFY
>Mature_815_residues
MFFIEVILPLSLAKTFTYRVSEAEFHFIKKGMRVAVPFGKSKIYTALVLDIHENAPTLYEAKEIHQILDERPIATEIQIK
HWLWVANYYMCGIGDVYRGAFPTGLLLESETIVSHKPDVVVNDSELSDDEFLIYEALQHQSSLKVQEIASILNRKNILPI
LQKLIARDIIFLEEEIKESYKPKLVRYVKLHSKYETDQGLGSLLDILKNDKQKEIVLAYFQISASEKKPVTVKKLVEVSG
SSSSVVKTLVKNEILEEYLLQQDRVLFTGEKSEKELLLSEAQQNAFTAIKQSLTEKEVCLLHGVTSSGKTEIYIKLIEEY
LHTGRQVLYLLPEIALTTQLVSRLRLHFGDKVAVFHSKYSNNERVEVWKQTLENSEKAQIVIGARSALFLPFNDLGLLIV
DEEHEQTFKQTDPAPRYHARDAAIVLANFHNAKVLLGSATPSIETYFNTQNDKYGLVTLTERYKNVRLPEVVLVDLKDKH
FRKRMTGHFSDLLIEEITEALSLGEQVILFQNRRGYSPIIECLTCGHVPHCQQCDVSLTYHKFKNQLRCHYCGYAIAKPT
NCHSCSSIDLTTKGFGTEQIEQELASLFPKAKTARMDQDTTRGKFGFEKIIDTFKNREIDILVGTQMLAKGLDFDNVSLV
GIMNADNMLHHPDFRAFERSFQMMTQVAGRAGRSEKQGKVVIQTYNPNHNTIQQVTNHNYNGMYKEQLYDRQIYKYPPYF
RIIKLTIKHKDFDKLKEGSMWLYQVLSQNLNIPVLGPEEPAISRIRNEYIRTILIKIPQNMHLGNTKKTIQKMLNSFEAV
AQYRAIKVVVNVDFY

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=550, Percent_Identity=38.3636363636364, Blast_Score=370, Evalue=1e-103,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 93607; Mature: 93607

Theoretical pI: Translated: 8.11; Mature: 8.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFFIEVILPLSLAKTFTYRVSEAEFHFIKKGMRVAVPFGKSKIYTALVLDIHENAPTLYE
CEEEEEHHHHHHHHHHHEEECHHHHHHHHCCCEEEEECCCCCEEEEEEEEECCCCCCHHH
AKEIHQILDERPIATEIQIKHWLWVANYYMCGIGDVYRGAFPTGLLLESETIVSHKPDVV
HHHHHHHHHCCCCCEEEEEHHEEEEEHHHEECCCHHHHCCCCCCEEEECCHHHCCCCCEE
VNDSELSDDEFLIYEALQHQSSLKVQEIASILNRKNILPILQKLIARDIIFLEEEIKESY
ECCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KPKLVRYVKLHSKYETDQGLGSLLDILKNDKQKEIVLAYFQISASEKKPVTVKKLVEVSG
CHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHCC
SSSSVVKTLVKNEILEEYLLQQDRVLFTGEKSEKELLLSEAQQNAFTAIKQSLTEKEVCL
CCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCHHHEEE
LHGVTSSGKTEIYIKLIEEYLHTGRQVLYLLPEIALTTQLVSRLRLHFGDKVAVFHSKYS
EECCCCCCCCHHHHHHHHHHHHCCHHEEHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCC
NNERVEVWKQTLENSEKAQIVIGARSALFLPFNDLGLLIVDEEHEQTFKQTDPAPRYHAR
CCHHHHHHHHHHCCCCCEEEEEEECCEEEEEECCCEEEEEECHHHHHHHHCCCCCCCCCC
DAAIVLANFHNAKVLLGSATPSIETYFNTQNDKYGLVTLTERYKNVRLPEVVLVDLKDKH
CCEEEEEECCCCEEEEECCCCCEEEEECCCCCCEEEEEEHHHHCCCCCCEEEEEECCHHH
FRKRMTGHFSDLLIEEITEALSLGEQVILFQNRRGYSPIIECLTCGHVPHCQQCDVSLTY
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCEEHH
HKFKNQLRCHYCGYAIAKPTNCHSCSSIDLTTKGFGTEQIEQELASLFPKAKTARMDQDT
HHHHCCCEEEECCEEECCCCCCCCCCCCCEECCCCCHHHHHHHHHHHCCCHHHHCCCCCC
TRGKFGFEKIIDTFKNREIDILVGTQMLAKGLDFDNVSLVGIMNADNMLHHPDFRAFERS
CCCCCCHHHHHHHHCCCEEEEEECHHHHHCCCCCCCEEEEEEECCCCCCCCCCHHHHHHH
FQMMTQVAGRAGRSEKQGKVVIQTYNPNHNTIQQVTNHNYNGMYKEQLYDRQIYKYPPYF
HHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHCCCCCCCCHHHHHHCHHHCCCCCE
RIIKLTIKHKDFDKLKEGSMWLYQVLSQNLNIPVLGPEEPAISRIRNEYIRTILIKIPQN
EEEEEEEECCCHHHHHCCCHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHEECCCC
MHLGNTKKTIQKMLNSFEAVAQYRAIKVVVNVDFY
CCCCCHHHHHHHHHHHHHHHHHHEEEEEEEEECCC
>Mature Secondary Structure
MFFIEVILPLSLAKTFTYRVSEAEFHFIKKGMRVAVPFGKSKIYTALVLDIHENAPTLYE
CEEEEEHHHHHHHHHHHEEECHHHHHHHHCCCEEEEECCCCCEEEEEEEEECCCCCCHHH
AKEIHQILDERPIATEIQIKHWLWVANYYMCGIGDVYRGAFPTGLLLESETIVSHKPDVV
HHHHHHHHHCCCCCEEEEEHHEEEEEHHHEECCCHHHHCCCCCCEEEECCHHHCCCCCEE
VNDSELSDDEFLIYEALQHQSSLKVQEIASILNRKNILPILQKLIARDIIFLEEEIKESY
ECCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KPKLVRYVKLHSKYETDQGLGSLLDILKNDKQKEIVLAYFQISASEKKPVTVKKLVEVSG
CHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHCC
SSSSVVKTLVKNEILEEYLLQQDRVLFTGEKSEKELLLSEAQQNAFTAIKQSLTEKEVCL
CCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCHHHEEE
LHGVTSSGKTEIYIKLIEEYLHTGRQVLYLLPEIALTTQLVSRLRLHFGDKVAVFHSKYS
EECCCCCCCCHHHHHHHHHHHHCCHHEEHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCC
NNERVEVWKQTLENSEKAQIVIGARSALFLPFNDLGLLIVDEEHEQTFKQTDPAPRYHAR
CCHHHHHHHHHHCCCCCEEEEEEECCEEEEEECCCEEEEEECHHHHHHHHCCCCCCCCCC
DAAIVLANFHNAKVLLGSATPSIETYFNTQNDKYGLVTLTERYKNVRLPEVVLVDLKDKH
CCEEEEEECCCCEEEEECCCCCEEEEECCCCCCEEEEEEHHHHCCCCCCEEEEEECCHHH
FRKRMTGHFSDLLIEEITEALSLGEQVILFQNRRGYSPIIECLTCGHVPHCQQCDVSLTY
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCEEHH
HKFKNQLRCHYCGYAIAKPTNCHSCSSIDLTTKGFGTEQIEQELASLFPKAKTARMDQDT
HHHHCCCEEEECCEEECCCCCCCCCCCCCEECCCCCHHHHHHHHHHHCCCHHHHCCCCCC
TRGKFGFEKIIDTFKNREIDILVGTQMLAKGLDFDNVSLVGIMNADNMLHHPDFRAFERS
CCCCCCHHHHHHHHCCCEEEEEECHHHHHCCCCCCCEEEEEEECCCCCCCCCCHHHHHHH
FQMMTQVAGRAGRSEKQGKVVIQTYNPNHNTIQQVTNHNYNGMYKEQLYDRQIYKYPPYF
HHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHCCCCCCCCHHHHHHCHHHCCCCCE
RIIKLTIKHKDFDKLKEGSMWLYQVLSQNLNIPVLGPEEPAISRIRNEYIRTILIKIPQN
EEEEEEEECCCHHHHHCCCHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHEECCCC
MHLGNTKKTIQKMLNSFEAVAQYRAIKVVVNVDFY
CCCCCHHHHHHHHHHHHHHHHHHEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377; 9086272 [H]