Click here to switch to the map view.

The map label for this gene is dapA

Identifier: 146301212

GI number: 146301212

Start: 4136702

End: 4137583

Strand: Reverse

Name: dapA

Synonym: Fjoh_3470

Alternate gene names: 146301212

Gene position: 4137583-4136702 (Counterclockwise)

Preceding gene: 146301213

Following gene: 146301211

Centisome position: 67.86

GC content: 35.83

Gene sequence:

>882_bases
ATGCAATCATTAATAGGAACGGGTGTTGCGCTTGTAACTCCATTTAAAAAAGACTTTTCAGTAGATATCGAAGCATTGCA
GCGCGTTGTTAATTTTTCGATTGATGGAGGAGTTGAGTATCTTGTTGTTATGGGAACAACTGCAGAAAATGCAACCCTTA
CAGCAGAAGAGAAAGAGTTGGTTATTAACACCGTAATTGATGTTAATAAAGGAAGACTGCCTTTAGTTCTTGGAGTTGGA
GGTAATAATACCATGCAGATTGTAGAGGAATTAAAAACAAGAGATTTTTCTGCTTTTGAAGCTATACTTTCAGTTTCACC
ATACTATAATAAACCTACACAAGAAGGAATCTATCAGCATTTTAAAGCTATCGCCGAGGCATCTCCGGTTCCTGTAATCT
TATATAATGTTCCGGGAAGAACAGCCAGTAATATGCTGCCTTCAACCGTAATTCGTTTAGCTAATGATTTTGATAATGTT
GTAGCTATAAAAGAAGCAGCAGGAGATATGGCTCAGGCTTTAAAAATTATTAAAGATGCACCAAAAGATTTTCTTGTAAT
TTCTGGAGACGATATGATCGCTCTGCCTATTGTTTTAGCCGGTGGTGCAGGAGTAATTTCTGTAATTGGACAAGGTTATC
CTAAAGAATTTTCAGAAATGATTCGTTTAGGATTGAATAAAAAAGCAGTTGAAGCTTTTAAAACGCAATATTTTTTATCA
GACTGTATCGACATGATTTTCGAGCAGGGAAATCCAGCCGGAATCAAACAAGTGTTTCAGGCACTTGGTATTACAGAGAA
CACAGTTCGTCTTCCATTAGTTTCTGTAGATGAATCTTTAGCAGAAAGAATAAATGACTTTGTAAAAAACAGCATTAAAT
AA

Upstream 100 bases:

>100_bases
AACCGATGGATGATAGAAACGACAACAGCAGAGTATAAACTTTTCATTTCAGAATTGTTTAGATATTTAAAAAGTTTAAA
ATAAAATTATAAATCAAAAT

Downstream 100 bases:

>100_bases
TTGTTAAAGTGTTAGTATTTTAATATACTAAAAAATTATTTTGCAGTCGCTAAATTAATACCTAAATTTGCCACCGAAAC
TTCGGTGTGGTTTTGCTTTG

Product: dihydrodipicolinate synthase

Products: NA

Alternate protein names: DHDPS

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MQSLIGTGVALVTPFKKDFSVDIEALQRVVNFSIDGGVEYLVVMGTTAENATLTAEEKELVINTVIDVNKGRLPLVLGVG
GNNTMQIVEELKTRDFSAFEAILSVSPYYNKPTQEGIYQHFKAIAEASPVPVILYNVPGRTASNMLPSTVIRLANDFDNV
VAIKEAAGDMAQALKIIKDAPKDFLVISGDDMIALPIVLAGGAGVISVIGQGYPKEFSEMIRLGLNKKAVEAFKTQYFLS
DCIDMIFEQGNPAGIKQVFQALGITENTVRLPLVSVDESLAERINDFVKNSIK

Sequences:

>Translated_293_residues
MQSLIGTGVALVTPFKKDFSVDIEALQRVVNFSIDGGVEYLVVMGTTAENATLTAEEKELVINTVIDVNKGRLPLVLGVG
GNNTMQIVEELKTRDFSAFEAILSVSPYYNKPTQEGIYQHFKAIAEASPVPVILYNVPGRTASNMLPSTVIRLANDFDNV
VAIKEAAGDMAQALKIIKDAPKDFLVISGDDMIALPIVLAGGAGVISVIGQGYPKEFSEMIRLGLNKKAVEAFKTQYFLS
DCIDMIFEQGNPAGIKQVFQALGITENTVRLPLVSVDESLAERINDFVKNSIK
>Mature_293_residues
MQSLIGTGVALVTPFKKDFSVDIEALQRVVNFSIDGGVEYLVVMGTTAENATLTAEEKELVINTVIDVNKGRLPLVLGVG
GNNTMQIVEELKTRDFSAFEAILSVSPYYNKPTQEGIYQHFKAIAEASPVPVILYNVPGRTASNMLPSTVIRLANDFDNV
VAIKEAAGDMAQALKIIKDAPKDFLVISGDDMIALPIVLAGGAGVISVIGQGYPKEFSEMIRLGLNKKAVEAFKTQYFLS
DCIDMIFEQGNPAGIKQVFQALGITENTVRLPLVSVDESLAERINDFVKNSIK

Specific function: Biosynthesis of diaminopimelate and lysine from aspartate semialdehyde; first step. [C]

COG id: COG0329

COG function: function code EM; Dihydrodipicolinate synthase/N-acetylneuraminate lyase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHDPS family

Homologues:

Organism=Homo sapiens, GI31543060, Length=289, Percent_Identity=25.2595155709343, Blast_Score=83, Evalue=3e-16,
Organism=Escherichia coli, GI1788823, Length=287, Percent_Identity=35.8885017421603, Blast_Score=192, Evalue=2e-50,
Organism=Escherichia coli, GI87082415, Length=196, Percent_Identity=29.5918367346939, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1786463, Length=219, Percent_Identity=28.7671232876712, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1789620, Length=283, Percent_Identity=27.9151943462898, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: 840 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): DAPA_FLAJ1 (A5FE82)

Other databases:

- EMBL:   CP000685
- RefSeq:   YP_001195803.1
- ProteinModelPortal:   A5FE82
- SMR:   A5FE82
- STRING:   A5FE82
- GeneID:   5089671
- GenomeReviews:   CP000685_GR
- KEGG:   fjo:Fjoh_3470
- eggNOG:   COG0329
- HOGENOM:   HBG358848
- OMA:   HFETIAS
- ProtClustDB:   PRK03170
- BioCyc:   FJOH376686:FJOH_3470-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00418
- InterPro:   IPR013785
- InterPro:   IPR005263
- InterPro:   IPR002220
- InterPro:   IPR020625
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR12128
- PRINTS:   PR00146
- TIGRFAMs:   TIGR00674

Pfam domain/function: PF00701 DHDPS

EC number: =4.2.1.52

Molecular weight: Translated: 31717; Mature: 31717

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS00665 DHDPS_1; PS00666 DHDPS_2

Important sites: ACT_SITE 164-164 BINDING 108-108

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSLIGTGVALVTPFKKDFSVDIEALQRVVNFSIDGGVEYLVVMGTTAENATLTAEEKEL
CCCHHHCCCCEECCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEEECCCCCCEEECCHHHE
VINTVIDVNKGRLPLVLGVGGNNTMQIVEELKTRDFSAFEAILSVSPYYNKPTQEGIYQH
EEEHEEECCCCCEEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHH
FKAIAEASPVPVILYNVPGRTASNMLPSTVIRLANDFDNVVAIKEAAGDMAQALKIIKDA
HHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCHHHHEEHHHHHHHHHHHHHHHHHC
PKDFLVISGDDMIALPIVLAGGAGVISVIGQGYPKEFSEMIRLGLNKKAVEAFKTQYFLS
CCCEEEECCCCEEEEEEEEECCCHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHH
DCIDMIFEQGNPAGIKQVFQALGITENTVRLPLVSVDESLAERINDFVKNSIK
HHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQSLIGTGVALVTPFKKDFSVDIEALQRVVNFSIDGGVEYLVVMGTTAENATLTAEEKEL
CCCHHHCCCCEECCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEEECCCCCCEEECCHHHE
VINTVIDVNKGRLPLVLGVGGNNTMQIVEELKTRDFSAFEAILSVSPYYNKPTQEGIYQH
EEEHEEECCCCCEEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHH
FKAIAEASPVPVILYNVPGRTASNMLPSTVIRLANDFDNVVAIKEAAGDMAQALKIIKDA
HHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCHHHHEEHHHHHHHHHHHHHHHHHC
PKDFLVISGDDMIALPIVLAGGAGVISVIGQGYPKEFSEMIRLGLNKKAVEAFKTQYFLS
CCCEEEECCCCEEEEEEEEECCCHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHH
DCIDMIFEQGNPAGIKQVFQALGITENTVRLPLVSVDESLAERINDFVKNSIK
HHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA