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The map label for this gene is pcm

Identifier: 146301188

GI number: 146301188

Start: 4113295

End: 4113936

Strand: Reverse

Name: pcm

Synonym: Fjoh_3446

Alternate gene names: 146301188

Gene position: 4113936-4113295 (Counterclockwise)

Preceding gene: 146301197

Following gene: 146301184

Centisome position: 67.48

GC content: 34.89

Gene sequence:

>642_bases
TTGAAAGACACTGCCAAACATCAAGGACTTCGTAATCAATTAGTAACCACTTTAGAGCAAAAAGGAATTACTGACAGAGC
TGTTTTAGATGCGATAAAAAAAATCCCAAGACACCTTTTTTTGAATTCTAGTTTTGAAGATTTTGCTTATCAGGACAAAG
CGTTTCCTATTGGTGCCGGGCAGACTATTTCTCAGCCTTACACGGTTGCTTTTCAGTCACAATTACTTGAAGTTAAGAAG
GATCACAAAATCCTGGAAATAGGAACAGGTTCTGGTTATCAAACTGCCGTTTTATTTATGCTTGGTGCTAAAGTATATAC
GGTAGAGAGACAAAGTGAACTATTTAAAACAACATCTAATTTATTTCCGAAATTAAATATCCGTCCTAAACACGTTACGT
TTGGAGATGGTTATAAAGGATTGCCAAATTTTGCACCATTTGACAGTATTATAGTTACAGCTGGTGCGCCATTTATTCCG
CAGCCATTAATGGCACAATTAAAAATAGGAGGAAGGCTTGTTATTCCGTTAGGGGAAGATGTTCAGATTATGACTTTATT
GATTAGAAAGAACGAAACTCAATTTGAAAAACATGAGTTTGGAGAATTTAGATTTGTTCCTCTATTAGAAGATAAAAATT
AA

Upstream 100 bases:

>100_bases
TTCGATATTTAACTTTCGACTGTATTAATTTATTATTTAAAAATCAATATTTGAAATCCTGTTTTGTTTCTTATTTTTGC
GAAAATAAAACCTACCCATT

Downstream 100 bases:

>100_bases
TAGAAAGCCTGGTTTTTAACCGGGCTTTTTTATTTTTCGACTAATTGTATTGCTCTTTTTAGATTTTCTTTTTCAATTCT
AGTGATATAATTAATAAAAA

Product: protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MKDTAKHQGLRNQLVTTLEQKGITDRAVLDAIKKIPRHLFLNSSFEDFAYQDKAFPIGAGQTISQPYTVAFQSQLLEVKK
DHKILEIGTGSGYQTAVLFMLGAKVYTVERQSELFKTTSNLFPKLNIRPKHVTFGDGYKGLPNFAPFDSIIVTAGAPFIP
QPLMAQLKIGGRLVIPLGEDVQIMTLLIRKNETQFEKHEFGEFRFVPLLEDKN

Sequences:

>Translated_213_residues
MKDTAKHQGLRNQLVTTLEQKGITDRAVLDAIKKIPRHLFLNSSFEDFAYQDKAFPIGAGQTISQPYTVAFQSQLLEVKK
DHKILEIGTGSGYQTAVLFMLGAKVYTVERQSELFKTTSNLFPKLNIRPKHVTFGDGYKGLPNFAPFDSIIVTAGAPFIP
QPLMAQLKIGGRLVIPLGEDVQIMTLLIRKNETQFEKHEFGEFRFVPLLEDKN
>Mature_213_residues
MKDTAKHQGLRNQLVTTLEQKGITDRAVLDAIKKIPRHLFLNSSFEDFAYQDKAFPIGAGQTISQPYTVAFQSQLLEVKK
DHKILEIGTGSGYQTAVLFMLGAKVYTVERQSELFKTTSNLFPKLNIRPKHVTFGDGYKGLPNFAPFDSIIVTAGAPFIP
QPLMAQLKIGGRLVIPLGEDVQIMTLLIRKNETQFEKHEFGEFRFVPLLEDKN

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family

Homologues:

Organism=Homo sapiens, GI226530908, Length=211, Percent_Identity=31.2796208530806, Blast_Score=79, Evalue=4e-15,
Organism=Escherichia coli, GI1789100, Length=197, Percent_Identity=43.6548223350254, Blast_Score=157, Evalue=5e-40,
Organism=Drosophila melanogaster, GI17981723, Length=227, Percent_Identity=31.7180616740088, Blast_Score=89, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PIMT_FLAJ1 (A5FEA5)

Other databases:

- EMBL:   CP000685
- RefSeq:   YP_001195779.1
- ProteinModelPortal:   A5FEA5
- SMR:   A5FEA5
- STRING:   A5FEA5
- GeneID:   5089647
- GenomeReviews:   CP000685_GR
- KEGG:   fjo:Fjoh_3446
- eggNOG:   COG2518
- HOGENOM:   HBG699907
- OMA:   YSVERIR
- ProtClustDB:   CLSK847580
- BioCyc:   FJOH376686:FJOH_3446-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00090
- InterPro:   IPR000682
- PANTHER:   PTHR11579
- TIGRFAMs:   TIGR00080

Pfam domain/function: PF01135 PCMT

EC number: =2.1.1.77

Molecular weight: Translated: 23954; Mature: 23954

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: PS01279 PCMT

Important sites: ACT_SITE 64-64

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDTAKHQGLRNQLVTTLEQKGITDRAVLDAIKKIPRHLFLNSSFEDFAYQDKAFPIGAG
CCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCC
QTISQPYTVAFQSQLLEVKKDHKILEIGTGSGYQTAVLFMLGAKVYTVERQSELFKTTSN
CCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCEEEEEHHHHHHHHHHHH
LFPKLNIRPKHVTFGDGYKGLPNFAPFDSIIVTAGAPFIPQPLMAQLKIGGRLVIPLGED
CCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHCCCEEEEECCCC
VQIMTLLIRKNETQFEKHEFGEFRFVPLLEDKN
CEEEEEEECCCCCHHHHHHCCCEEEEEEECCCC
>Mature Secondary Structure
MKDTAKHQGLRNQLVTTLEQKGITDRAVLDAIKKIPRHLFLNSSFEDFAYQDKAFPIGAG
CCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCC
QTISQPYTVAFQSQLLEVKKDHKILEIGTGSGYQTAVLFMLGAKVYTVERQSELFKTTSN
CCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCEEEEEHHHHHHHHHHHH
LFPKLNIRPKHVTFGDGYKGLPNFAPFDSIIVTAGAPFIPQPLMAQLKIGGRLVIPLGED
CCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHCCCEEEEECCCC
VQIMTLLIRKNETQFEKHEFGEFRFVPLLEDKN
CEEEEEEECCCCCHHHHHHCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA