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The map label for this gene is argG [H]

Identifier: 146301177

GI number: 146301177

Start: 4102912

End: 4104105

Strand: Reverse

Name: argG [H]

Synonym: Fjoh_3435

Alternate gene names: 146301177

Gene position: 4104105-4102912 (Counterclockwise)

Preceding gene: 146301178

Following gene: 146301176

Centisome position: 67.31

GC content: 34.59

Gene sequence:

>1194_bases
ATGAAAAAAGTAGTATTAGCTTATAGCGGAGGATTAGATACCTCGTATTGTTTGAAATATTTAAAAAACGAAAAAGGTTA
TGAAGTTCATACCGTTCTTGTTGATACAGGAGGGTTTAGTGCAGAAGAATTATCAGCTATAGAAAAAAGAGCTTACGAAT
TAGGAAGTGCGCAGCATGCTAACTTAACAATTTTAGACAAATATTACGATAAAGCTATAAAATACCTGATTTTTGGAAAT
GTATTAAAAAATAATACATACCCATTATCAGTAAGTGCAGAGCGTGTTTTTCAAGCAATTGAAGCTATAAAATATGCAAA
AAAAGTTGGAGCAACTGCAATCGCTCACGGAAGTACAGGCGCAGGAAACGACCAAATTCGTTTCGATTTGATTTTCCAAA
CTATTGCTCCAGAAATCGAAATTATTACTCCAATTAGAGATTTAAAACTTTCAAGACAAGAAGAAGTTGATTATTTGGCT
CAAAACGGAGTTCACTACTCTTGGGAAAAAGCACAATATTCTATCAATAAAGGACTTTGGGGAACAAGTGTTGGAGGAAA
AGAAACTTTAACTTCTGGTCAGCCATTGCCAAGTGAAGCGTATCCTTCGCAATTGCAAAAAGAAGGTGAAGAAAAAATAA
CACTTCATTTTGAGCAGGGAGAATTAGTTGGTTTAAACGGAAAAACGGATAAACCATCAAATAATATTGTGGCTCTTGAA
AAGCTGGCAAGTGCTTATGCAATTGGTAGAGATATTCATGTTGGAGATACAATTATAGGAATTAAAGGTAGAGTTGGTTT
TGAAGCAGCAGCTCCGTTAATTATTATCAAAGCGCACCATTTATTAGAGAAACACACTCTAGGAAAATGGCAGCAATACT
GGAAAGAACAATTAGGAAACTGGTACGGAATGTTGTTCCACGAAGGTCAGTTCTTAGATCCAGTAATGAGAAATATTGAA
ACTTTCTTGCAAGACACACAAAAAACAGTAAATGGAACAGTAACTGTTTCATTAAAACCATATCATTTCTCACTTGACGG
AATCGAATCTGAAAATGATTTAATGAACACAGGTTTCGGTCAATACGGCGAAATGAACAATGCCTGGACATCTGACGATG
CAAAAGGATTTATCAAGATTTTAGGAAATGCACAAAACATATTCTCATCTGTAAACAAATTAGATCATGATTAA

Upstream 100 bases:

>100_bases
TTCTTAAACAAAATATTGTCATTTATTTTTTTATTCAAAATTTAATCAAAAAGAAATCTCAAACTGCTATATACGAAAGT
ATTAGCCTAAATTATAAAAA

Downstream 100 bases:

>100_bases
TGTCGGAATTATTGGTGGTTCGGGCTACACGGCCGGAGAACTCATCAGAATTTTAATGTATCACCCAAAAGTAAACATCG
ATTTTGTTTACAGTACAACA

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase [H]

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MKKVVLAYSGGLDTSYCLKYLKNEKGYEVHTVLVDTGGFSAEELSAIEKRAYELGSAQHANLTILDKYYDKAIKYLIFGN
VLKNNTYPLSVSAERVFQAIEAIKYAKKVGATAIAHGSTGAGNDQIRFDLIFQTIAPEIEIITPIRDLKLSRQEEVDYLA
QNGVHYSWEKAQYSINKGLWGTSVGGKETLTSGQPLPSEAYPSQLQKEGEEKITLHFEQGELVGLNGKTDKPSNNIVALE
KLASAYAIGRDIHVGDTIIGIKGRVGFEAAAPLIIIKAHHLLEKHTLGKWQQYWKEQLGNWYGMLFHEGQFLDPVMRNIE
TFLQDTQKTVNGTVTVSLKPYHFSLDGIESENDLMNTGFGQYGEMNNAWTSDDAKGFIKILGNAQNIFSSVNKLDHD

Sequences:

>Translated_397_residues
MKKVVLAYSGGLDTSYCLKYLKNEKGYEVHTVLVDTGGFSAEELSAIEKRAYELGSAQHANLTILDKYYDKAIKYLIFGN
VLKNNTYPLSVSAERVFQAIEAIKYAKKVGATAIAHGSTGAGNDQIRFDLIFQTIAPEIEIITPIRDLKLSRQEEVDYLA
QNGVHYSWEKAQYSINKGLWGTSVGGKETLTSGQPLPSEAYPSQLQKEGEEKITLHFEQGELVGLNGKTDKPSNNIVALE
KLASAYAIGRDIHVGDTIIGIKGRVGFEAAAPLIIIKAHHLLEKHTLGKWQQYWKEQLGNWYGMLFHEGQFLDPVMRNIE
TFLQDTQKTVNGTVTVSLKPYHFSLDGIESENDLMNTGFGQYGEMNNAWTSDDAKGFIKILGNAQNIFSSVNKLDHD
>Mature_397_residues
MKKVVLAYSGGLDTSYCLKYLKNEKGYEVHTVLVDTGGFSAEELSAIEKRAYELGSAQHANLTILDKYYDKAIKYLIFGN
VLKNNTYPLSVSAERVFQAIEAIKYAKKVGATAIAHGSTGAGNDQIRFDLIFQTIAPEIEIITPIRDLKLSRQEEVDYLA
QNGVHYSWEKAQYSINKGLWGTSVGGKETLTSGQPLPSEAYPSQLQKEGEEKITLHFEQGELVGLNGKTDKPSNNIVALE
KLASAYAIGRDIHVGDTIIGIKGRVGFEAAAPLIIIKAHHLLEKHTLGKWQQYWKEQLGNWYGMLFHEGQFLDPVMRNIE
TFLQDTQKTVNGTVTVSLKPYHFSLDGIESENDLMNTGFGQYGEMNNAWTSDDAKGFIKILGNAQNIFSSVNKLDHD

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI53759107, Length=392, Percent_Identity=27.8061224489796, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI16950633, Length=392, Percent_Identity=27.8061224489796, Blast_Score=106, Evalue=4e-23,
Organism=Escherichia coli, GI1789563, Length=359, Percent_Identity=27.2980501392758, Blast_Score=76, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6324514, Length=362, Percent_Identity=31.767955801105, Blast_Score=139, Evalue=8e-34,
Organism=Drosophila melanogaster, GI21358151, Length=408, Percent_Identity=28.1862745098039, Blast_Score=127, Evalue=9e-30,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00764 Arginosuc_synth [H]

EC number: =6.3.4.5 [H]

Molecular weight: Translated: 44133; Mature: 44133

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVVLAYSGGLDTSYCLKYLKNEKGYEVHTVLVDTGGFSAEELSAIEKRAYELGSAQHA
CCEEEEEECCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCC
NLTILDKYYDKAIKYLIFGNVLKNNTYPLSVSAERVFQAIEAIKYAKKVGATAIAHGSTG
CEEEEHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHCCEEEECCCCC
AGNDQIRFDLIFQTIAPEIEIITPIRDLKLSRQEEVDYLAQNGVHYSWEKAQYSINKGLW
CCCCCEEEEEEEHHHCCCEEEECCHHHHCCCCHHHHHHHHHCCCCEEHHHHHEECCCCCC
GTSVGGKETLTSGQPLPSEAYPSQLQKEGEEKITLHFEQGELVGLNGKTDKPSNNIVALE
CCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEEEECCCEEECCCCCCCCCCCEEHHH
KLASAYAIGRDIHVGDTIIGIKGRVGFEAAAPLIIIKAHHLLEKHTLGKWQQYWKEQLGN
HHHHHHHCCCEEECCCEEEEECCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCC
WYGMLFHEGQFLDPVMRNIETFLQDTQKTVNGTVTVSLKPYHFSLDGIESENDLMNTGFG
EEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECEEEEECCCCCCCCHHHCCCC
QYGEMNNAWTSDDAKGFIKILGNAQNIFSSVNKLDHD
CCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MKKVVLAYSGGLDTSYCLKYLKNEKGYEVHTVLVDTGGFSAEELSAIEKRAYELGSAQHA
CCEEEEEECCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCC
NLTILDKYYDKAIKYLIFGNVLKNNTYPLSVSAERVFQAIEAIKYAKKVGATAIAHGSTG
CEEEEHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHCCEEEECCCCC
AGNDQIRFDLIFQTIAPEIEIITPIRDLKLSRQEEVDYLAQNGVHYSWEKAQYSINKGLW
CCCCCEEEEEEEHHHCCCEEEECCHHHHCCCCHHHHHHHHHCCCCEEHHHHHEECCCCCC
GTSVGGKETLTSGQPLPSEAYPSQLQKEGEEKITLHFEQGELVGLNGKTDKPSNNIVALE
CCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEEEECCCEEECCCCCCCCCCCEEHHH
KLASAYAIGRDIHVGDTIIGIKGRVGFEAAAPLIIIKAHHLLEKHTLGKWQQYWKEQLGN
HHHHHHHCCCEEECCCEEEEECCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCC
WYGMLFHEGQFLDPVMRNIETFLQDTQKTVNGTVTVSLKPYHFSLDGIESENDLMNTGFG
EEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECEEEEECCCCCCCCHHHCCCC
QYGEMNNAWTSDDAKGFIKILGNAQNIFSSVNKLDHD
CCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]