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The map label for this gene is yafV [C]

Identifier: 146301158

GI number: 146301158

Start: 4082323

End: 4083213

Strand: Reverse

Name: yafV [C]

Synonym: Fjoh_3416

Alternate gene names: 146301158

Gene position: 4083213-4082323 (Counterclockwise)

Preceding gene: 146301159

Following gene: 146301155

Centisome position: 66.97

GC content: 36.14

Gene sequence:

>891_bases
ATGTCGAAAAGAAAGTATAAAATATCAGTTATTCAGTTAAACCTGAATGATGTAGCCGAAAACAATCTTAAAAAATGTAT
CAGCTGGGTAAGAGATGCTGCAAGCCAAGGAGCAGAGGTAATCTTACTTCCTGAATTATATAGCAGTCATTATTTCTGCC
AAAGTGAAGATGTGGATAATTTTGCATTAGCAGAACCACTTTACAGTACTTCATTTATTGCTTTCAGCGAGTTGGCAAAA
GAATTAGGAGTGGTAATTATTGTTCCTTTCTTCGAAAAAAGAATGGCAGGAATTTATCATAATAGTGCATACATTATCGA
TACAGATGGTACAGAAGCTGGTCTATACCGTAAAATGCACATTCCGGATGATCCGCATTTCTATGAAAAATTCTATTTTA
CCCCAGGTGACTTAGGTTTTCAGGCAATCGAAACTAAAAAAGGAACAATTGGAACATTAATATGCTGGGATCAATGGTAT
CCAGAAGCGGCACGTATTACTGCTTTAAAAGGTGCTGAGGTTTTATTTTATCCAACAGCAATTGGATGGCATCCAAAAGA
AAAAGAGCAATATGGAGAAAACCAATACGGAGCATGGATGAATGTAATGAAAGGCCACGCCGTTGCAAATGGTGTTTTTG
TTGCAGCAGCAAACAGAATTGGTCTTGAAAAATATATTGAAGGAACAGAAGGAATTCAATTTTGGGGAGCGTCATTTATT
GCAGGACCTCAAGGAGAAATTTTAGCTCAGGCTTCTCATGATAAAGAAGAAATCTTGATTGCTGAGGTTGATTTAGATTT
ACAGGAAAATGTTCGTCAAAATTGGCCATTCTTTAGAGATAGAAGAATTGATGCTTTTGGTGATATTACAAAAAGAGCGA
TCGATAAATAA

Upstream 100 bases:

>100_bases
TAAGAGGGCTTTTTTAAAGCTCTTTTTTTGATCATAGTTGAAACTGTAAACTTTGCCATTCCTTTAAAAAATACTATTTT
TGCATTCTAAATTTTATGTC

Downstream 100 bases:

>100_bases
TTCAGTTTATTTAAAATATAAAAAGGGTAAAATTGATATTTTGCCCTTTTTTTATAAGCTTCGAACAAGCGAATATTTAT
AACAAGAAATACACATTTGC

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: D-N-alpha-carbamilase [H]

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MSKRKYKISVIQLNLNDVAENNLKKCISWVRDAASQGAEVILLPELYSSHYFCQSEDVDNFALAEPLYSTSFIAFSELAK
ELGVVIIVPFFEKRMAGIYHNSAYIIDTDGTEAGLYRKMHIPDDPHFYEKFYFTPGDLGFQAIETKKGTIGTLICWDQWY
PEAARITALKGAEVLFYPTAIGWHPKEKEQYGENQYGAWMNVMKGHAVANGVFVAAANRIGLEKYIEGTEGIQFWGASFI
AGPQGEILAQASHDKEEILIAEVDLDLQENVRQNWPFFRDRRIDAFGDITKRAIDK

Sequences:

>Translated_296_residues
MSKRKYKISVIQLNLNDVAENNLKKCISWVRDAASQGAEVILLPELYSSHYFCQSEDVDNFALAEPLYSTSFIAFSELAK
ELGVVIIVPFFEKRMAGIYHNSAYIIDTDGTEAGLYRKMHIPDDPHFYEKFYFTPGDLGFQAIETKKGTIGTLICWDQWY
PEAARITALKGAEVLFYPTAIGWHPKEKEQYGENQYGAWMNVMKGHAVANGVFVAAANRIGLEKYIEGTEGIQFWGASFI
AGPQGEILAQASHDKEEILIAEVDLDLQENVRQNWPFFRDRRIDAFGDITKRAIDK
>Mature_295_residues
SKRKYKISVIQLNLNDVAENNLKKCISWVRDAASQGAEVILLPELYSSHYFCQSEDVDNFALAEPLYSTSFIAFSELAKE
LGVVIIVPFFEKRMAGIYHNSAYIIDTDGTEAGLYRKMHIPDDPHFYEKFYFTPGDLGFQAIETKKGTIGTLICWDQWYP
EAARITALKGAEVLFYPTAIGWHPKEKEQYGENQYGAWMNVMKGHAVANGVFVAAANRIGLEKYIEGTEGIQFWGASFIA
GPQGEILAQASHDKEEILIAEVDLDLQENVRQNWPFFRDRRIDAFGDITKRAIDK

Specific function: The enzyme catalyzes the hydrolysis of N-carbamoyl-D- amino acids to the corresponding which are useful intermediates in the preparation of beta-lactam antibiotics. Industrial production of beta-lactam antibiotics is now being developed using this enzyme

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=289, Percent_Identity=30.1038062283737, Blast_Score=129, Evalue=2e-30,
Organism=Homo sapiens, GI297632348, Length=293, Percent_Identity=26.6211604095563, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI297632350, Length=293, Percent_Identity=26.6211604095563, Blast_Score=111, Evalue=6e-25,
Organism=Homo sapiens, GI5031947, Length=293, Percent_Identity=26.6211604095563, Blast_Score=111, Evalue=6e-25,
Organism=Homo sapiens, GI7706509, Length=279, Percent_Identity=26.8817204301075, Blast_Score=101, Evalue=9e-22,
Organism=Homo sapiens, GI297632346, Length=186, Percent_Identity=29.5698924731183, Blast_Score=81, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17533173, Length=278, Percent_Identity=32.3741007194245, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17556280, Length=301, Percent_Identity=23.5880398671096, Blast_Score=95, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6323383, Length=301, Percent_Identity=27.5747508305648, Blast_Score=105, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6322335, Length=320, Percent_Identity=24.0625, Blast_Score=80, Evalue=3e-16,
Organism=Drosophila melanogaster, GI21358471, Length=283, Percent_Identity=28.2685512367491, Blast_Score=111, Evalue=5e-25,
Organism=Drosophila melanogaster, GI17933642, Length=296, Percent_Identity=28.7162162162162, Blast_Score=102, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.77 [H]

Molecular weight: Translated: 33541; Mature: 33410

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKRKYKISVIQLNLNDVAENNLKKCISWVRDAASQGAEVILLPELYSSHYFCQSEDVDN
CCCCEEEEEEEEEECHHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHCCCEEECCCCCCC
FALAEPLYSTSFIAFSELAKELGVVIIVPFFEKRMAGIYHNSAYIIDTDGTEAGLYRKMH
HHHHCCHHHHHHHHHHHHHHHCCEEEEEECHHHHHHHEEECCEEEEECCCCCCCCEEEEC
IPDDPHFYEKFYFTPGDLGFQAIETKKGTIGTLICWDQWYPEAARITALKGAEVLFYPTA
CCCCCCHHHEEEECCCCCCCEEEECCCCCEEEEEEECCCCCCHHEEEEECCCEEEEEECC
IGWHPKEKEQYGENQYGAWMNVMKGHAVANGVFVAAANRIGLEKYIEGTEGIQFWGASFI
CCCCCCCHHHCCCCCHHHHHHHHHCCHHHCCEEEEECCCCCHHHHHCCCCCEEEECCEEC
AGPQGEILAQASHDKEEILIAEVDLDLQENVRQNWPFFRDRRIDAFGDITKRAIDK
CCCCCCEEEECCCCCCEEEEEEECCCHHHHHHHCCCCHHCCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
SKRKYKISVIQLNLNDVAENNLKKCISWVRDAASQGAEVILLPELYSSHYFCQSEDVDN
CCCEEEEEEEEEECHHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHCCCEEECCCCCCC
FALAEPLYSTSFIAFSELAKELGVVIIVPFFEKRMAGIYHNSAYIIDTDGTEAGLYRKMH
HHHHCCHHHHHHHHHHHHHHHCCEEEEEECHHHHHHHEEECCEEEEECCCCCCCCEEEEC
IPDDPHFYEKFYFTPGDLGFQAIETKKGTIGTLICWDQWYPEAARITALKGAEVLFYPTA
CCCCCCHHHEEEECCCCCCCEEEECCCCCEEEEEEECCCCCCHHEEEEECCCEEEEEECC
IGWHPKEKEQYGENQYGAWMNVMKGHAVANGVFVAAANRIGLEKYIEGTEGIQFWGASFI
CCCCCCCHHHCCCCCHHHHHHHHHCCHHHCCEEEEECCCCCHHHHHCCCCCEEEECCEEC
AGPQGEILAQASHDKEEILIAEVDLDLQENVRQNWPFFRDRRIDAFGDITKRAIDK
CCCCCCEEEECCCCCCEEEEEEECCCHHHHHHHCCCCHHCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9648217; 10903946 [H]